FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3793900
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT82650.21784970610717203Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA10150.062.6163672
CGAGTCG407.611934E-659.2916451
GAGCGGC9000.055.8541079
GATCGGG13100.051.778351
TCGGGAG13350.043.6956833
AGCGGCG7250.039.2468710-11
CGACCGG500.001627477137.9466551
GGCGCCG6350.037.0278956-57
AGAGCGG14350.035.6909338
AAGCGGC3750.035.4093979
CGCCGTA13100.032.6332358-59
CGGGAGA18250.032.4834254
TTGTGTT321450.032.2124521
CGCCGGA3350.031.90263758-59
CGACCCG600.003979358431.6222111
TCGCCGT12600.031.47845556-57
GATCCGG3650.031.189031
GTCGCCG14000.031.0449256-57
GTGTTTG712800.030.9900321
TGTTTGA723700.030.4749832
Produced by FastQC (version 0.11.2)