##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2757354 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21927869979698 33.0 33.0 33.0 27.0 33.0 2 30.77753527475979 33.0 33.0 33.0 27.0 33.0 3 30.618949543656708 33.0 33.0 33.0 27.0 33.0 4 34.039945904660776 37.0 37.0 37.0 27.0 37.0 5 34.11130707192475 37.0 37.0 37.0 27.0 37.0 6 34.70058070164368 37.0 37.0 37.0 27.0 37.0 7 34.07089477810974 37.0 37.0 37.0 27.0 37.0 8 34.362934537966474 37.0 37.0 37.0 27.0 37.0 9 33.83401224507263 37.0 37.0 37.0 27.0 37.0 10-11 33.85403016805242 37.0 37.0 37.0 24.5 37.0 12-13 33.835908991010946 37.0 37.0 37.0 24.5 37.0 14-15 35.37794276686998 40.0 37.0 40.0 22.0 40.0 16-17 35.303230198226274 40.0 37.0 40.0 22.0 40.0 18-19 35.366365725982234 40.0 37.0 40.0 22.0 40.0 20-21 35.33470856480524 40.0 37.0 40.0 24.5 40.0 22-23 36.090756391816214 40.0 37.0 40.0 27.0 40.0 24-25 35.3748368907293 40.0 37.0 40.0 22.0 40.0 26-27 35.25629589091571 40.0 37.0 40.0 24.5 40.0 28-29 34.91497754731529 38.5 37.0 40.0 18.5 40.0 30-31 34.948474878452316 40.0 37.0 40.0 18.5 40.0 32-33 34.74073550222424 37.0 37.0 40.0 15.0 40.0 34-35 34.996857131873526 37.0 37.0 40.0 22.0 40.0 36-37 34.63463958563173 37.0 37.0 40.0 15.0 40.0 38-39 34.587497289067706 37.0 37.0 40.0 15.0 40.0 40-41 35.07064435687256 37.0 37.0 40.0 22.0 40.0 42-43 34.674833372863986 37.0 37.0 40.0 18.5 40.0 44-45 34.23128604451949 37.0 35.0 40.0 15.0 40.0 46-47 34.07092125276624 37.0 33.0 40.0 15.0 40.0 48-49 33.96171692136737 37.0 33.0 40.0 15.0 40.0 50-51 33.00375523055799 37.0 33.0 38.5 15.0 38.5 52-53 33.46922448115113 37.0 33.0 38.5 15.0 40.0 54-55 34.07808881268056 37.0 33.0 40.0 15.0 40.0 56-57 34.3698467806455 37.0 33.0 40.0 18.5 40.0 58-59 33.846746373516055 37.0 33.0 40.0 15.0 40.0 60-61 33.65462178595857 37.0 33.0 40.0 15.0 40.0 62-63 33.29810880285956 37.0 33.0 40.0 15.0 40.0 64-65 33.05029405002042 37.0 33.0 38.5 15.0 40.0 66-67 32.92690166006976 37.0 33.0 37.0 15.0 40.0 68-69 32.992939064044734 37.0 33.0 37.0 15.0 40.0 70-71 32.59107336236116 37.0 33.0 37.0 15.0 40.0 72-73 32.80498514155237 37.0 33.0 37.0 22.0 38.5 74-75 32.85732553745366 37.0 33.0 37.0 22.0 37.0 76-77 32.56612136127606 37.0 33.0 37.0 22.0 37.0 78-79 32.12909785975975 37.0 33.0 37.0 22.0 37.0 80-81 31.6333918314442 37.0 33.0 37.0 15.0 37.0 82-83 31.26904144335475 35.0 33.0 37.0 15.0 37.0 84-85 30.994474594121755 33.0 33.0 37.0 15.0 37.0 86-87 30.822841390695572 33.0 33.0 37.0 15.0 37.0 88-89 30.672595539056644 33.0 33.0 37.0 15.0 37.0 90-91 30.531483443910357 33.0 27.0 37.0 15.0 37.0 92-93 30.3726743102264 33.0 27.0 37.0 15.0 37.0 94-95 30.218556268074394 33.0 27.0 37.0 15.0 37.0 96-97 30.08983920816841 33.0 27.0 37.0 15.0 37.0 98-99 29.930418800052514 33.0 27.0 37.0 15.0 37.0 100-101 28.68388716138733 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.15907358730405008 2306 2 0.0029512478876121406 2306 3 0.05376057889035479 2306 4 0.2980556368660032 2306 5 0.27705654603360585 2306 6 0.1101252096441101 2306 7 0.6207238004657896 2306 8 0.16474550630623952 2306 9 0.30114379625746324 2306 10-11 0.2028275599700109 2306 12-13 0.32830148215557386 2306 14-15 0.6692293607023743 2306 16-17 0.4469871389140039 2306 18-19 0.406934330328788 2306 20-21 0.4149079115424783 2306 22-23 0.4795159008028662 2306 24-25 0.743353383977194 2306 26-27 0.40483346876997217 2306 28-29 0.5155916623027608 2306 30-31 0.5550022657923321 2306 32-33 0.6536036539968748 2306 34-35 0.5560141636668519 2306 36-37 0.6369574149631987 2306 38-39 0.5838318589938822 2306 40-41 0.33776542901962614 2306 42-43 0.5016560002575261 2306 44-45 0.6760002625291861 2306 46-47 0.39886142953074 2306 48-49 0.6796933553765783 2306 50-51 0.5514571682220222 2306 52-53 0.5343485950907407 2306 54-55 0.7575679452869792 2306 56-57 0.8240026219070771 2306 58-59 0.9894287149623935 2306 60-61 0.9002201252514865 2306 62-63 0.8504955996350603 2306 64-65 0.7820529280852782 2306 66-67 0.6615390926432312 2306 68-69 0.578179886500962 2306 70-71 0.6986233069419967 2306 72-73 0.3975117327727773 2306 74-75 0.5236981055679735 2306 76-77 0.6359850307352986 2306 78-79 0.7716393918294671 2306 80-81 0.8809357819268655 2306 82-83 0.7276637511336936 2306 84-85 0.7019773769806683 2306 86-87 0.9474449590964618 2306 88-89 0.8166076632130022 2306 90-91 0.8114312153990362 2306 92-93 0.7081707722487032 2306 94-95 0.6605076757349728 2306 96-97 0.5931925805835547 2306 98-99 0.6215943318900479 2306 100-101 0.7085295503352427 2307 1 0.01331215401786423 2307 2 0.04059386929261066 2307 3 0.04137962650940352 2307 4 0.21926732946383254 2307 5 0.22680194914534724 2307 6 0.00966316343949103 2307 7 0.45568843978686147 2307 8 -0.015749543198708693 2307 9 0.19112022247151828 2307 10-11 0.09599116204450553 2307 12-13 0.18925857786514655 2307 14-15 0.5147314823431159 2307 16-17 0.4712516367923669 2307 18-19 0.4104845424928598 2307 20-21 0.33280748721433184 2307 22-23 0.2615998234808501 2307 24-25 0.571679645174747 2307 26-27 0.3022403523154722 2307 28-29 0.4605657311382103 2307 30-31 0.5009805779092602 2307 32-33 0.5112910655951737 2307 34-35 0.3785271292491217 2307 36-37 0.568763166966967 2307 38-39 0.41894737054503395 2307 40-41 0.20722558932136792 2307 42-43 0.5413566131759637 2307 44-45 0.521787626979922 2307 46-47 0.2214899066641678 2307 48-49 0.25329778724833574 2307 50-51 0.19175419792498616 2307 52-53 0.24888419150752128 2307 54-55 0.4392511136038024 2307 56-57 0.4314637251602633 2307 58-59 0.5348011807803985 2307 60-61 0.4561159291175798 2307 62-63 0.5602763634257215 2307 64-65 0.580812946944306 2307 66-67 0.6538375367733522 2307 68-69 0.37854763756178755 2307 70-71 0.48466526828098466 2307 72-73 0.22927269458325128 2307 74-75 0.2766778321120569 2307 76-77 0.41751025468626324 2307 78-79 0.43653330979269356 2307 80-81 0.519707587678873 2307 82-83 0.627654321619314 2307 84-85 0.6114540389325747 2307 86-87 0.5641611307136252 2307 88-89 0.5472535849480344 2307 90-91 0.5086400791425554 2307 92-93 0.4758864723901475 2307 94-95 0.5376741066637827 2307 96-97 0.5431878658263649 2307 98-99 0.44179235169202613 2307 100-101 0.3517662311461791 2201 1 0.26407423746287506 2201 2 0.18525392468437474 2201 3 0.20723149821493436 2201 4 0.34541134804687346 2201 5 0.37989495370176485 2201 6 0.1546100052156021 2201 7 0.6277575901486969 2201 8 0.13376265192324155 2201 9 0.3304733896295602 2201 10-11 0.31300626076691884 2201 12-13 0.39317665166127114 2201 14-15 0.5989086466422293 2201 16-17 0.45146069602581207 2201 18-19 0.5554979437257757 2201 20-21 0.5039908160805808 2201 22-23 0.358890540893519 2201 24-25 0.7048820931332855 2201 26-27 0.49661406803065233 2201 28-29 0.6254996183891777 2201 30-31 0.7605258294057293 2201 32-33 0.540986857608047 2201 34-35 0.4903425496011309 2201 36-37 0.7693255720415735 2201 38-39 0.5976872079434088 2201 40-41 0.40029491836630626 2201 42-43 0.8066371456516137 2201 44-45 0.8168139827379832 2201 46-47 0.7613040762344383 2201 48-49 0.7468120372260003 2201 50-51 0.7466134849793988 2201 52-53 0.6463723537517794 2201 54-55 0.843519406286731 2201 56-57 0.7464396029410167 2201 58-59 1.024088681897176 2201 60-61 0.9603203425902933 2201 62-63 0.8318067842244332 2201 64-65 0.9837670253861859 2201 66-67 0.7352058026538231 2201 68-69 0.762223595749937 2201 70-71 0.8425085631974554 2201 72-73 0.5911689690404955 2201 74-75 0.48948628382294856 2201 76-77 0.6162308836507933 2201 78-79 0.7434912299213394 2201 80-81 0.7979100356374147 2201 82-83 0.8132042188765318 2201 84-85 0.8337134868017806 2201 86-87 0.81824493309011 2201 88-89 0.8981738029414181 2201 90-91 0.9581920312554821 2201 92-93 0.90169054636495 2201 94-95 0.90256645009271 2201 96-97 0.8776138463499059 2201 98-99 0.8926460102286136 2201 100-101 0.7572236598962903 1101 1 0.03584801173444774 1101 2 -0.15205800805805936 1101 3 -0.1388543251703318 1101 4 -0.07859905671580947 1101 5 -0.0153807499048213 1101 6 0.02491528302896029 1101 7 0.15672942914110877 1101 8 -0.008663420931860344 1101 9 -0.08195493530243425 1101 10-11 0.004240030534333528 1101 12-13 -0.03889403257661428 1101 14-15 0.20199673382523997 1101 16-17 0.10047137287956787 1101 18-19 0.06955511047909368 1101 20-21 0.03637540969791786 1101 22-23 0.12629829992631159 1101 24-25 0.2895058707118139 1101 26-27 0.20753613863535492 1101 28-29 0.02385507900587669 1101 30-31 0.28770270451090596 1101 32-33 0.15319773991149077 1101 34-35 0.11343979578829533 1101 36-37 0.2642948173781363 1101 38-39 -0.0035782876432790545 1101 40-41 0.14091112511533765 1101 42-43 0.1405033588889708 1101 44-45 0.09582632144030612 1101 46-47 0.05304692840191194 1101 48-49 0.1057326058879724 1101 50-51 0.04761318944662918 1101 52-53 0.048042092954581506 1101 54-55 0.3198624478295926 1101 56-57 0.4395659754311865 1101 58-59 0.44614369368918005 1101 60-61 0.2666064626804072 1101 62-63 0.24358324173260115 1101 64-65 0.3031234588331273 1101 66-67 0.3244195473553617 1101 68-69 0.13330412382428847 1101 70-71 0.2771405662984563 1101 72-73 0.35393611015027915 1101 74-75 0.4738592562211963 1101 76-77 0.6045055374044352 1101 78-79 0.5303702448344438 1101 80-81 0.3634017293248206 1101 82-83 0.25483702066948766 1101 84-85 0.39451485361989924 1101 86-87 0.25582556623348296 1101 88-89 0.4114022430725939 1101 90-91 0.16952265707332614 1101 92-93 0.2305065399620183 1101 94-95 0.2684725384863107 1101 96-97 0.19581870664997325 1101 98-99 0.19836754133950407 1101 100-101 0.40504697316186267 2304 1 0.08095592773146976 2304 2 -0.17164256332878125 2304 3 -0.13473539246147936 2304 4 -0.08500985858238153 2304 5 -0.13998920061805364 2304 6 0.07277268298589235 2304 7 -0.06266301180541234 2304 8 0.03399083280845616 2304 9 -0.16598128240975285 2304 10-11 -0.0805893896031904 2304 12-13 -0.22642032395520317 2304 14-15 -0.05230905992571877 2304 16-17 0.06942147948324617 2304 18-19 0.01891656704842859 2304 20-21 -0.03329559502125079 2304 22-23 0.08386076904081108 2304 24-25 0.061402968702900296 2304 26-27 0.18573461841940997 2304 28-29 -0.05593168967433826 2304 30-31 0.002085152236752208 2304 32-33 -0.03547578438563903 2304 34-35 0.16084477430177202 2304 36-37 0.0335862474973041 2304 38-39 -0.14295355455195846 2304 40-41 -0.049340680875161524 2304 42-43 0.0875247079877397 2304 44-45 -0.03590751732668451 2304 46-47 -0.04688477893074605 2304 48-49 0.005593264078278537 2304 50-51 0.11700934564208154 2304 52-53 -0.03045502493106511 2304 54-55 0.2610058136933304 2304 56-57 0.41067462169434776 2304 58-59 0.13034963113637588 2304 60-61 0.005986543433138536 2304 62-63 0.10398975388485354 2304 64-65 0.2938297436361239 2304 66-67 0.2349271901817218 2304 68-69 0.3582451692154507 2304 70-71 0.22106207946741563 2304 72-73 0.3734426651677225 2304 74-75 0.48927044838865186 2304 76-77 0.47777350233089066 2304 78-79 0.43790688697647795 2304 80-81 0.34769411622045965 2304 82-83 0.2655988584313569 2304 84-85 0.31107995017442036 2304 86-87 0.22671559206844805 2304 88-89 0.1669896978354437 2304 90-91 0.10631518678632901 2304 92-93 0.09534867983355255 2304 94-95 0.09761932557497843 2304 96-97 0.35377830791188103 2304 98-99 0.2490394421127995 2304 100-101 0.18473570720876253 2202 1 0.1734673281037935 2202 2 0.03781140396432292 2202 3 0.13003349131398068 2202 4 0.10626277122506167 2202 5 0.09863203352125538 2202 6 0.23175413424736746 2202 7 0.14272073539349606 2202 8 0.16730544661799485 2202 9 0.033086084172609276 2202 10-11 0.15163077022993576 2202 12-13 0.24413298383935 2202 14-15 0.3175042492910336 2202 16-17 0.22538896585840718 2202 18-19 0.2807027560511912 2202 20-21 0.24562971590213323 2202 22-23 0.33330237415488995 2202 24-25 0.4480175367468462 2202 26-27 0.3491877870938609 2202 28-29 0.3969501749361015 2202 30-31 0.3754767470490137 2202 32-33 0.21742723085557714 2202 34-35 0.27735120062920515 2202 36-37 0.31350809956322934 2202 38-39 0.2638625097167946 2202 40-41 0.3673191786919432 2202 42-43 0.358270103623056 2202 44-45 0.1008182738618828 2202 46-47 0.30180752862130333 2202 48-49 0.28939370423019994 2202 50-51 0.4023040960919886 2202 52-53 0.4607908930883582 2202 54-55 0.6024894028095105 2202 56-57 0.5419670711014888 2202 58-59 0.38120696101510276 2202 60-61 0.45311379547390374 2202 62-63 0.4302167183864398 2202 64-65 0.45477328056858823 2202 66-67 0.5358625585947152 2202 68-69 0.5218455718067645 2202 70-71 0.5202466861842012 2202 72-73 0.5830827033117885 2202 74-75 0.5373028927269345 2202 76-77 0.4999401838880857 2202 78-79 0.49781349600159785 2202 80-81 0.4656381472754134 2202 82-83 0.4782308903323411 2202 84-85 0.4444261075971063 2202 86-87 0.48327857809991315 2202 88-89 0.5038533327901433 2202 90-91 0.5011475953873408 2202 92-93 0.41611777876198275 2202 94-95 0.6156835140927406 2202 96-97 0.5688071074130221 2202 98-99 0.59563145556168 2202 100-101 0.5652464949421265 1102 1 0.02775738937532779 1102 2 -0.1253538574408566 1102 3 -0.08953187032390275 1102 4 -0.17100462293537078 1102 5 -0.1283905164955712 1102 6 -0.03346191641230689 1102 7 -0.1635816691766152 1102 8 -0.002671735609595771 1102 9 -0.12405818925346779 1102 10-11 -0.03292340109773306 1102 12-13 -0.10026869950804951 1102 14-15 -0.24024469900715673 1102 16-17 -0.12022128660466791 1102 18-19 -0.09884251220790219 1102 20-21 -0.09800179932128827 1102 22-23 -0.016892665887453973 1102 24-25 -0.10338505759406047 1102 26-27 -0.0359456886781615 1102 28-29 -0.10461998132231543 1102 30-31 6.259446799603552E-4 1102 32-33 -0.0771166439815687 1102 34-35 -0.1351065380928631 1102 36-37 -0.04820675400257102 1102 38-39 -0.17506821652536786 1102 40-41 0.04502286614700779 1102 42-43 0.039281470355319925 1102 44-45 -0.1396240614976918 1102 46-47 -0.11439238722694256 1102 48-49 -0.06328373458998726 1102 50-51 -0.01828029805048459 1102 52-53 -0.06933059613233894 1102 54-55 0.21102190701124357 1102 56-57 0.20608454320768743 1102 58-59 0.13270057664453105 1102 60-61 0.19160580847293573 1102 62-63 0.12377715738070805 1102 64-65 0.1402517941581607 1102 66-67 0.06836855577351741 1102 68-69 0.09898705058265023 1102 70-71 0.07207003931167577 1102 72-73 0.21493523633432687 1102 74-75 0.1882421059626509 1102 76-77 0.2888359770027904 1102 78-79 0.09455663564276762 1102 80-81 0.10736040119983414 1102 82-83 -0.045849392629893515 1102 84-85 0.04445381991051178 1102 86-87 0.07418285040188621 1102 88-89 0.02000906506265565 1102 90-91 0.028342117872956152 1102 92-93 0.02675571621646 1102 94-95 0.11304772180550415 1102 96-97 0.18596360548213653 1102 98-99 0.1394792048924849 1102 100-101 0.23300708241194457 2305 1 0.2733469124467831 2305 2 0.16296786998778146 2305 3 0.17054890005823964 2305 4 0.1370705967561534 2305 5 0.106687246329912 2305 6 0.2551132850939908 2305 7 0.11701273592867523 2305 8 0.293082573589615 2305 9 0.10025126174853938 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pass #Quality Count 2 1380.0 3 124.0 4 231.0 5 236.0 6 437.0 7 2739.0 8 13294.0 9 39246.0 10 60423.0 11 46575.0 12 28274.0 13 18497.0 14 13616.0 15 10991.0 16 9688.0 17 9001.0 18 8940.0 19 9181.0 20 9714.0 21 10632.0 22 11751.0 23 12730.0 24 14403.0 25 16961.0 26 20262.0 27 24661.0 28 30879.0 29 36237.0 30 43464.0 31 55672.0 32 75508.0 33 107008.0 34 161493.0 35 269611.0 36 505187.0 37 822206.0 38 255269.0 39 833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.44457477984558 23.786921114952555 19.917594443619 14.85090966158286 2 32.21612326362212 26.66806880962059 27.746629135731432 13.369178791025849 3 36.76511066325327 20.302521923852062 26.549013199702237 16.38335421319244 4 30.560328425064416 18.515957925803704 29.932803000153957 20.99091064897792 5 35.83560758337555 16.73259722078474 28.519839994480062 18.91195520135965 6 33.66731370520614 17.018251717364265 31.821619320493298 17.492815256936293 7 36.506861502594624 19.14771386136097 24.989596081008944 19.35582855503546 8 29.54356885646571 30.459582926197815 21.526385104994667 18.470463112341807 9 32.78008961647764 25.85258557807203 22.2614635712032 19.105861234247133 10-11 27.31216120821209 30.207389629411267 26.287147862109578 16.19330130026707 12-13 29.027950748696007 25.230172115923917 26.20841836118652 19.53345877419356 14-15 26.18759928093775 25.448801130193587 27.954243936112327 20.409355652756336 16-17 27.82505909736301 25.883964204453548 28.552381930993743 17.738594767189703 18-19 27.257160845391677 26.044785033942965 28.90756192680287 17.79049219386249 20-21 25.574044632526117 28.35156927619094 27.956867641436716 18.117518449846227 22-23 28.55354587537885 26.03689681303124 27.11252736415596 18.297029947433952 24-25 24.85629345360212 29.48001254934293 27.278943014657347 18.3847509823976 26-27 23.286075979756045 30.98810130270393 27.41449090115131 18.311331816388712 28-29 24.52026801206666 29.938323293560153 27.02756574880349 18.513842945569696 30-31 26.52053817631193 27.47764701520706 27.47377605379655 18.528038754684452 32-33 22.708125470872105 30.01340621972965 29.085842711990622 18.192625597407623 34-35 27.270966872692977 27.647909017501238 27.348242648846327 17.73288146095946 36-37 23.68481388382569 29.247035186843455 29.525620803920056 17.542530125410803 38-39 23.130124236479215 31.45547410848924 27.428427087175034 17.98597456785651 40-41 27.635868759772908 26.90160619821689 26.88296732341576 18.57955771859445 42-43 27.427278704893403 26.77354301781321 27.2904401503714 18.50873812692199 44-45 24.05943707064776 28.726323287314514 28.62314327346868 18.591096368569048 46-47 24.637104372645844 29.207122802090463 27.536849794569534 18.618923030694155 48-49 21.285352551935535 29.477626125260088 29.167970993113702 20.069050329690675 50-51 20.10191150462438 27.87969393080138 30.541619957833362 21.47677460674088 52-53 25.562989462632263 27.227260184342544 27.067150548714746 20.142599804310446 54-55 26.77572692186965 27.019364821066084 27.35165949107558 18.85324876598868 56-57 27.25081361063786 26.902997677528813 26.725151931885332 19.121036779947993 58-59 25.646110893862367 27.282945771682687 26.778039486727113 20.292903847727832 60-61 21.907012952155743 27.36489539734498 26.683564762324387 24.044526888174893 62-63 24.62478849830844 27.3717965619667 26.791311360493435 21.21210357923142 64-65 22.13339850992206 28.77109942030298 29.024604344099654 20.0708977256753 66-67 20.871130107951988 28.707533071003322 27.831580203116026 22.589756617928664 68-69 20.69866422746412 27.311406575481467 32.52320167155689 19.466727525497515 70-71 20.11292900377006 31.22418959768179 29.667897899934314 18.99498349861384 72-73 19.889721608140505 34.547018562305276 26.448780918769664 19.114478910784555 74-75 20.211602427704804 34.41062855331075 26.184964141467766 19.19280487751668 76-77 20.47790523193898 33.830390018459426 26.10242961186493 19.589275137736664 78-79 20.192441936562876 33.63194616134146 26.656334986402175 19.51927691569349 80-81 19.64711854139373 33.581516456880934 26.80961330993129 19.96175169179405 82-83 19.612835918513444 32.98986528884163 26.864023321366847 20.533275471278074 84-85 19.729131217299805 32.426951189234366 27.35798350814362 20.48593408532221 86-87 20.0085988479217 32.00431906599925 27.937307705808095 20.049774380270954 88-89 20.529084149974473 32.17844067392151 27.53590423040693 19.756570945697085 90-91 20.92507420461544 31.665800196060566 26.913849909834326 20.495275689489667 92-93 20.749557287216057 31.888629099411293 26.75583344545088 20.60598016792178 94-95 20.386877033863964 31.870045034276988 27.111151023619502 20.63192690823955 96-97 20.687158422263526 32.23664883699961 26.734756798105924 20.341435942630937 98-99 21.030079751323946 31.669286740028717 26.595599222232767 20.705034286414563 100-101 21.21646131090102 31.63379428907025 26.269313184740184 20.880431215288542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1164.0 1 679.5 2 150.5 3 108.5 4 102.5 5 91.0 6 125.5 7 212.5 8 328.5 9 472.5 10 634.5 11 774.0 12 830.0 13 831.5 14 782.5 15 729.5 16 651.0 17 569.5 18 546.0 19 580.0 20 667.0 21 783.5 22 995.0 23 1365.5 24 1866.0 25 2547.0 26 3485.5 27 4666.5 28 6227.0 29 8256.0 30 10670.0 31 13920.5 32 19643.0 33 28745.5 34 41758.0 35 60419.5 36 86666.5 37 118665.0 38 149615.0 39 169473.5 40 176197.5 41 169625.0 42 159152.5 43 156025.0 44 149391.0 45 142221.5 46 143676.0 47 137907.0 48 119144.5 49 100272.0 50 85470.0 51 75709.0 52 68473.0 53 59628.5 54 50892.0 55 43221.0 56 35918.5 57 29088.0 58 23321.0 59 18828.5 60 15367.0 61 12379.0 62 9843.0 63 7801.5 64 6096.5 65 4803.5 66 3784.5 67 2927.5 68 2258.5 69 1717.0 70 1339.0 71 1074.5 72 805.5 73 589.5 74 474.5 75 383.5 76 276.0 77 199.0 78 158.0 79 129.5 80 101.0 81 77.0 82 59.0 83 60.5 84 50.5 85 36.0 86 36.5 87 28.0 88 25.0 89 23.0 90 13.5 91 13.0 92 12.5 93 7.0 94 9.0 95 7.5 96 3.0 97 2.0 98 2.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1678058022292386 2 0.16047993837570365 3 0.12707835120191316 4 0.12192848651279452 5 0.13415034848626617 6 0.1558740734776891 7 0.12997968342113492 8 0.12174715324909315 9 0.11507408914488311 10-11 0.10170982761009287 12-13 0.1305418165386091 14-15 0.1265524847371792 16-17 0.11650662192812386 18-19 0.11514662245036364 20-21 0.10861862495711468 22-23 0.11949862077919628 24-25 0.41320410799628915 26-27 0.2969332193109771 28-29 0.3848617188797666 30-31 0.22120844838928916 32-33 0.25887136726006166 34-35 0.2706398960742799 36-37 0.2680649637297206 38-39 0.2872318897029544 40-41 0.28014175909223116 42-43 0.33455987152900935 44-45 0.2825353581730891 46-47 0.30797641507039 48-49 0.3078857484385393 50-51 0.3082121483132017 52-53 0.3123465467255927 54-55 0.2896617554365526 56-57 0.3025545504857193 58-59 0.30206495067372563 60-61 0.2805044256196339 62-63 0.2782196264969968 64-65 0.286524689974519 66-67 0.28618015677348646 68-69 0.28365962440803755 70-71 0.25859936736450956 72-73 0.24880737112463616 74-75 0.2575657677614118 76-77 0.2537577692236833 78-79 0.2507476370462407 80-81 0.2599231001895295 82-83 0.3005417512586342 84-85 0.30541961605220075 86-87 0.2955188198541065 88-89 0.30244575052749845 90-91 0.2790174928572827 92-93 0.2634409655053359 94-95 0.25000417066506514 96-97 0.249587104158552 98-99 0.26949749651296134 100-101 0.2702409628941369 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2757354.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.01761023115165 #Duplication Level Percentage of deduplicated Percentage of total 1 91.31121017787018 54.802806221901804 2 4.584320246933246 5.5027989151043295 3 1.1535197785049565 2.0769450138070455 4 0.6680268849432389 1.603735088178148 5 0.39174858341656194 1.1755906894050507 6 0.23632438506878492 0.8510174898704956 7 0.21955659245175935 0.9224083392614654 8 0.18267068321044586 0.8770766292466173 9 0.12142912978553681 0.6559097563958647 >10 0.9209954521824162 10.808046151606117 >50 0.10292652381473479 4.385215179879183 >100 0.09900842664474307 11.714900848031657 >500 0.006044985742426356 2.3547967080236227 >1k 0.0021576356767313686 1.8665210499478633 >5k 0.0 0.0 >10k+ 6.051375430591737E-5 0.40223191934085173 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 11075 0.40165317909851256 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2778 0.10074876131247565 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00467839820349509 0.0 0.0 2 0.005113598036378354 0.0 0.0 3 0.00576639778570325 0.0 0.0 4 0.006274130924067058 0.0 0.0 5 0.008051196908340387 0.0 0.0 6 0.009646929628912356 0.0 0.0 7 0.012294395278952213 0.0 0.0 8 0.014687994359810165 0.0 0.0 9 0.016646393607784856 0.0 0.0 10-11 0.025948790035664627 0.0 0.0 12-13 0.035740786275538075 0.0 1.8133326370136006E-5 14-15 0.045605315820892056 0.0 3.626665274027201E-5 16-17 0.05467197900596006 0.0 3.626665274027201E-5 18-19 0.06339410898999548 0.0 3.626665274027201E-5 20-21 0.0701034397469458 0.0 3.626665274027201E-5 22-23 0.07643197065012328 0.0 3.626665274027201E-5 24-25 0.08504530067593788 0.0 3.626665274027201E-5 26-27 0.09318716421612894 0.0 3.626665274027201E-5 28-29 0.10292476047689197 0.0 3.626665274027201E-5 30-31 0.2024404555961984 0.0 3.626665274027201E-5 32-33 0.21517005070803386 0.0 3.626665274027201E-5 34-35 0.2248532469896865 0.0 3.626665274027201E-5 36-37 0.23611404266554095 0.0 3.626665274027201E-5 38-39 0.24976843742225335 0.0 3.626665274027201E-5 40-41 0.2658889645653043 0.0 3.626665274027201E-5 42-43 0.2836233577552973 0.0 3.626665274027201E-5 44-45 0.3038238833316288 0.0 3.626665274027201E-5 46-47 0.32616414141963634 0.0 3.626665274027201E-5 48-49 0.3499187989645145 0.0 3.626665274027201E-5 50-51 0.37802545483822536 0.0 3.626665274027201E-5 52-53 0.4102302424715869 0.0 5.439997911040802E-5 54-55 0.444375296026553 0.0 9.066663185068003E-5 56-57 0.48289048123672185 0.0 1.0879995822081605E-4 58-59 0.5245064652561839 0.0 1.0879995822081605E-4 60-61 0.5696221812650824 0.0 1.2693328459095204E-4 62-63 0.6181469626315663 0.0 1.8133326370136008E-4 64-65 0.6704616092094087 0.0 1.8133326370136008E-4 66-67 0.7252061215208494 0.0 1.8133326370136008E-4 68-69 0.7822716996076673 0.0 1.994665900714961E-4 70-71 0.8449767421955977 0.0 2.175999164416321E-4 72-73 0.9127772494935362 0.0 2.357332428117681E-4 74-75 0.9844582886346838 0.0 2.538665691819041E-4 76-77 1.061669992318723 0.0 2.538665691819041E-4 78-79 1.1439952940391405 0.0 2.538665691819041E-4 80-81 1.2342992593624178 0.0 2.538665691819041E-4 82-83 1.3338149544817242 0.0 2.538665691819041E-4 84-85 1.4411642465929293 0.0 2.538665691819041E-4 86-87 1.552321537241863 0.0 2.901332219221761E-4 88-89 1.6695534922247923 0.0 3.082665482923121E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2625 0.0 87.77098 9 AGAGCGG 2775 0.0 84.90581 8 CGGGAGA 3935 0.0 82.40983 4 GTTCGGA 540 0.0 81.65605 2 ATCGGGA 4610 0.0 80.73612 2 GAGAGCG 3645 0.0 80.50764 7 TCGGGAG 4005 0.0 79.54894 3 AAGCGGC 800 0.0 77.036644 9 TCGGAGA 910 0.0 76.58498 4 GATCGGG 5040 0.0 75.45677 1 CGGAAAG 1410 0.0 73.63641 5 GAACCGG 330 0.0 71.8473 1 TTCGGAG 660 0.0 71.83295 3 GATCCGG 810 0.0 70.83612 1 TCGGGAA 1085 0.0 69.912994 3 CGGAGAG 1625 0.0 68.270035 5 AAAGCGG 1000 0.0 67.79225 8 AAGCGTC 330 0.0 67.519295 9 ATTCGGA 635 0.0 67.1998 2 ATCCGGA 840 0.0 66.60425 2 >>END_MODULE