FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2757354
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT110750.40165317909851256Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC27780.10074876131247565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC26250.087.770989
AGAGCGG27750.084.905818
CGGGAGA39350.082.409834
GTTCGGA5400.081.656052
ATCGGGA46100.080.736122
GAGAGCG36450.080.507647
TCGGGAG40050.079.548943
AAGCGGC8000.077.0366449
TCGGAGA9100.076.584984
GATCGGG50400.075.456771
CGGAAAG14100.073.636415
GAACCGG3300.071.84731
TTCGGAG6600.071.832953
GATCCGG8100.070.836121
TCGGGAA10850.069.9129943
CGGAGAG16250.068.2700355
AAAGCGG10000.067.792258
AAGCGTC3300.067.5192959
ATTCGGA6350.067.19982
ATCCGGA8400.066.604252
Produced by FastQC (version 0.11.2)