##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2329468 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.067199463568507 33.0 33.0 33.0 27.0 33.0 2 30.676036760324674 33.0 33.0 33.0 27.0 33.0 3 30.48117338379407 33.0 33.0 33.0 27.0 33.0 4 33.94728410091918 37.0 37.0 37.0 27.0 37.0 5 34.03048764782345 37.0 37.0 37.0 27.0 37.0 6 34.54165071166464 37.0 37.0 37.0 27.0 37.0 7 33.9105336497432 37.0 37.0 37.0 27.0 37.0 8 34.22225031638125 37.0 37.0 37.0 27.0 37.0 9 33.695737395834584 37.0 37.0 37.0 22.0 37.0 10-11 33.707798304162154 37.0 37.0 37.0 22.0 37.0 12-13 33.67566199664473 37.0 37.0 37.0 22.0 37.0 14-15 35.12747932145881 38.5 37.0 40.0 22.0 40.0 16-17 35.04328413182752 37.0 37.0 40.0 18.5 40.0 18-19 35.08838155321301 38.5 37.0 40.0 18.5 40.0 20-21 35.105821372090105 38.5 37.0 40.0 18.5 40.0 22-23 35.730512932566576 37.0 37.0 40.0 27.0 40.0 24-25 35.072714027408836 37.0 37.0 40.0 22.0 40.0 26-27 34.959050736047885 37.0 37.0 40.0 21.0 40.0 28-29 34.63700488695273 37.0 37.0 40.0 15.0 40.0 30-31 34.688981346813954 37.0 37.0 40.0 18.5 40.0 32-33 34.47733667086219 37.0 37.0 40.0 15.0 40.0 34-35 34.561123612773386 37.0 37.0 40.0 18.5 40.0 36-37 34.414255100306164 37.0 37.0 40.0 15.0 40.0 38-39 34.552680697910425 37.0 35.0 40.0 18.5 40.0 40-41 34.17562035623584 37.0 33.0 40.0 15.0 40.0 42-43 34.01171340409054 37.0 33.0 40.0 15.0 40.0 44-45 33.895368384541015 37.0 33.0 40.0 15.0 40.0 46-47 33.804276341207526 37.0 33.0 40.0 15.0 40.0 48-49 33.68882981006822 37.0 33.0 40.0 15.0 40.0 50-51 32.706538789114084 37.0 33.0 38.5 15.0 38.5 52-53 33.19857924642021 37.0 33.0 38.5 15.0 40.0 54-55 33.79504032680423 37.0 33.0 40.0 15.0 40.0 56-57 34.02145425479122 37.0 33.0 40.0 18.5 40.0 58-59 33.51408282921251 37.0 33.0 40.0 15.0 40.0 60-61 33.359605927190245 37.0 33.0 40.0 15.0 40.0 62-63 32.99888751423072 37.0 33.0 40.0 15.0 40.0 64-65 32.75829567094289 37.0 33.0 37.0 15.0 40.0 66-67 32.61554247579276 37.0 33.0 37.0 15.0 40.0 68-69 32.65683623900393 37.0 33.0 37.0 15.0 40.0 70-71 32.252126021907145 37.0 33.0 37.0 15.0 38.5 72-73 32.45994858053427 37.0 33.0 37.0 22.0 37.0 74-75 32.45230692158037 37.0 33.0 37.0 22.0 37.0 76-77 32.11839119489943 37.0 33.0 37.0 22.0 37.0 78-79 31.66824098892966 33.0 33.0 37.0 18.5 37.0 80-81 31.20392939503784 33.0 33.0 37.0 15.0 37.0 82-83 30.894980527742817 33.0 33.0 37.0 15.0 37.0 84-85 30.677930111081157 33.0 27.0 37.0 15.0 37.0 86-87 30.525733987331012 33.0 27.0 37.0 15.0 37.0 88-89 30.392094246411627 33.0 27.0 37.0 15.0 37.0 90-91 30.25503312344278 33.0 27.0 37.0 15.0 37.0 92-93 30.085903090319334 33.0 27.0 37.0 15.0 37.0 94-95 29.925677236175815 33.0 27.0 37.0 15.0 37.0 96-97 29.782926402079788 33.0 27.0 37.0 15.0 37.0 98-99 29.609813914593374 33.0 27.0 37.0 15.0 37.0 100-101 28.36414559032363 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14758507551047728 2306 2 0.021138494184491208 2306 3 0.12933028469400298 2306 4 0.163805803424232 2306 5 0.16745964774468547 2306 6 -0.016439481086038654 2306 7 0.42917945042575667 2306 8 0.06832954860018958 2306 9 0.21315103324550932 2306 10-11 0.057260775574270895 2306 12-13 0.17656065783683061 2306 14-15 0.556050688749373 2306 16-17 0.4921170341111889 2306 18-19 0.40766888980720495 2306 20-21 0.3651647785621819 2306 22-23 0.42250692937574286 2306 24-25 0.6931706646210358 2306 26-27 0.3650622668689465 2306 28-29 0.3920815394589283 2306 30-31 0.49729062725502615 2306 32-33 0.6279077608608361 2306 34-35 0.44184185010926313 2306 36-37 0.37456994281471623 2306 38-39 0.3620303974984864 2306 40-41 0.5415510077586418 2306 42-43 0.3918299735794619 2306 44-45 0.5194775053933611 2306 46-47 0.41397638970724415 2306 48-49 0.5153723606671932 2306 50-51 0.3863179933424874 2306 52-53 0.529367920437636 2306 54-55 0.6666589660867857 2306 56-57 0.6066757350961964 2306 58-59 0.7999701427202197 2306 60-61 0.6958227558953922 2306 62-63 0.6193272926178963 2306 64-65 0.7020732826201765 2306 66-67 0.6700890390410805 2306 68-69 0.6589354456193917 2306 70-71 0.6448924842316544 2306 72-73 0.4154795853790816 2306 74-75 0.5623286041885223 2306 76-77 0.7027768104875847 2306 78-79 0.6227135930390482 2306 80-81 0.6444786620841398 2306 82-83 0.5901950084691805 2306 84-85 0.6383217698115047 2306 86-87 0.6360260001248541 2306 88-89 0.6551168720162259 2306 90-91 0.6369278921987451 2306 92-93 0.5711094606397786 2306 94-95 0.6502442427813229 2306 96-97 0.6608454150644505 2306 98-99 0.6819180993634433 2306 100-101 0.3112298747770552 2307 1 0.14300517370756793 2307 2 -0.056084907483676005 2307 3 0.14179848894920966 2307 4 0.16711486135000087 2307 5 0.15629646573827927 2307 6 0.2089494622107253 2307 7 0.5459853501385226 2307 8 0.020826870198945358 2307 9 0.11885619220780086 2307 10-11 0.16224013709599916 2307 12-13 0.16304114001478354 2307 14-15 0.4541763008380073 2307 16-17 0.522674798845685 2307 18-19 0.5741420433535325 2307 20-21 0.43922503766053467 2307 22-23 0.31615221703274443 2307 24-25 0.5112023847790397 2307 26-27 0.17808406215365125 2307 28-29 0.3209395874795433 2307 30-31 0.4894874420937043 2307 32-33 0.449530674829937 2307 34-35 0.30656845599977345 2307 36-37 0.3168029078541821 2307 38-39 0.22195132715019383 2307 40-41 0.3548127089213722 2307 42-43 0.07796850772167829 2307 44-45 0.20571134461041396 2307 46-47 0.17245640521896632 2307 48-49 0.22866774724430172 2307 50-51 0.370289815904151 2307 52-53 0.3084800057784065 2307 54-55 0.42807273471235163 2307 56-57 0.3350304737302423 2307 58-59 0.3021731688206657 2307 60-61 0.25957922185176585 2307 62-63 0.2734742136067041 2307 64-65 0.2780360281950607 2307 66-67 0.41220106522697364 2307 68-69 0.2530646530098082 2307 70-71 0.2683102556703645 2307 72-73 -0.014004354072618241 2307 74-75 0.2032184249492559 2307 76-77 0.28477861142486205 2307 78-79 0.34074629911162546 2307 80-81 0.3282020341748684 2307 82-83 0.4536200428118704 2307 84-85 0.4659669785953433 2307 86-87 0.14953631607761153 2307 88-89 0.13541238347583828 2307 90-91 0.21155373762048768 2307 92-93 0.23743193357642056 2307 94-95 0.14590490413281287 2307 96-97 0.19848976938900265 2307 98-99 0.2001128240632326 2307 100-101 0.06820390578735669 2201 1 0.008553295073305378 2201 2 -0.14523306115397006 2201 3 -0.03536218894950238 2201 4 0.030021153283804836 2201 5 0.005910364152875047 2201 6 0.08053511898722832 2201 7 0.2564451930886733 2201 8 -0.05958849186058757 2201 9 0.08805690290806467 2201 10-11 -0.011820079841527331 2201 12-13 -0.07284021528565177 2201 14-15 0.06313336062628139 2201 16-17 0.09334785187485295 2201 18-19 0.12299585280634773 2201 20-21 0.04781261407324422 2201 22-23 0.04572036101167498 2201 24-25 0.23644327116640085 2201 26-27 0.09297661843812932 2201 28-29 0.08377288353838708 2201 30-31 0.19685521084277013 2201 32-33 -0.07567262644733574 2201 34-35 0.044547588498154767 2201 36-37 0.040148386458625396 2201 38-39 -0.03266573959599839 2201 40-41 0.05909741147094394 2201 42-43 -0.0473020429467752 2201 44-45 -0.04698360096883647 2201 46-47 -0.20015600842846482 2201 48-49 -0.07034603189192268 2201 50-51 -0.04600674614544431 2201 52-53 -0.13699409466391899 2201 54-55 0.05775906988456114 2201 56-57 0.16624879041430773 2201 58-59 0.20511474727104684 2201 60-61 0.03061383742752355 2201 62-63 0.03595961443171092 2201 64-65 0.06862126873976848 2201 66-67 -0.004102330479007321 2201 68-69 0.018087204075861507 2201 70-71 0.12846165222123318 2201 72-73 -0.11318183034119045 2201 74-75 0.11105631819511785 2201 76-77 0.23749436859399253 2201 78-79 0.06941038141370015 2201 80-81 0.14191361038877304 2201 82-83 -0.031455096346224565 2201 84-85 -0.03709838222171413 2201 86-87 0.017338456875780395 2201 88-89 0.04721488459407297 2201 90-91 0.13709452833665736 2201 92-93 -0.09706265541020898 2201 94-95 -0.04177401341601694 2201 96-97 -0.032301133150635764 2201 98-99 0.011826487648541217 2201 100-101 -0.007435662325562475 1101 1 0.3756883998120806 1101 2 0.17857166566044214 1101 3 0.2410133986823979 1101 4 0.3350210842859269 1101 5 0.313076540934631 1101 6 0.2939765949897648 1101 7 0.38085583624086894 1101 8 0.31776836054835655 1101 9 0.453846261595821 1101 10-11 0.4021814001628883 1101 12-13 0.40098112851696044 1101 14-15 0.49340065839351865 1101 16-17 0.5387297091136531 1101 18-19 0.49331607194359606 1101 20-21 0.581231303527602 1101 22-23 0.5381842118750626 1101 24-25 0.7140073184437483 1101 26-27 0.7055341454162942 1101 28-29 0.6353381578279382 1101 30-31 0.7414886423830751 1101 32-33 0.6896775320177824 1101 34-35 0.6257582309603649 1101 36-37 0.7058403143972498 1101 38-39 0.5144299098580021 1101 40-41 0.38949201031729785 1101 42-43 0.41863514368787946 1101 44-45 0.41941098407794897 1101 46-47 0.5356046988917527 1101 48-49 0.37708649968610075 1101 50-51 0.46979707334691767 1101 52-53 0.5341041042615515 1101 54-55 0.8562792379633848 1101 56-57 0.8313926164844574 1101 58-59 0.6200305928629959 1101 60-61 0.591253194735323 1101 62-63 0.7752635063718358 1101 64-65 0.6717496611352871 1101 66-67 0.6478279713177031 1101 68-69 0.6791084071228113 1101 70-71 0.7013765939410277 1101 72-73 0.556914030706281 1101 74-75 0.7349238905738886 1101 76-77 0.6796485627448092 1101 78-79 0.6521043344399438 1101 80-81 0.5353586110327555 1101 82-83 0.5801801590379085 1101 84-85 0.6098485480628355 1101 86-87 0.7044295007216306 1101 88-89 0.669000877335499 1101 90-91 0.7403501355639541 1101 92-93 0.5667203313238005 1101 94-95 0.709121234010361 1101 96-97 0.6595592450497172 1101 98-99 0.5351392883642809 1101 100-101 0.7051102787594488 2304 1 0.11937887357885657 2304 2 -0.017193260708470604 2304 3 0.007931380756506456 2304 4 -0.11759095805435038 2304 5 -0.05870610553656519 2304 6 0.0772145799691657 2304 7 -0.10583757968796448 2304 8 -0.03450726176541963 2304 9 -0.1111052535323509 2304 10-11 0.02004863805762369 2304 12-13 0.004666066815197212 2304 14-15 0.07273600048932138 2304 16-17 0.12853567058216697 2304 18-19 0.08416763719912268 2304 20-21 0.11389634550307193 2304 22-23 0.13909096123994402 2304 24-25 0.25827098282486816 2304 26-27 0.17313294610146102 2304 28-29 0.039255684849109684 2304 30-31 0.1344682541409412 2304 32-33 0.2189351794656602 2304 34-35 0.2310969317539886 2304 36-37 0.16108486820601797 2304 38-39 0.11470360191097484 2304 40-41 0.2093466244824853 2304 42-43 0.15368318836340222 2304 44-45 0.07990793269218699 2304 46-47 -0.017420797828513912 2304 48-49 0.14526867052519066 2304 50-51 0.08692670730717822 2304 52-53 0.05367083004291828 2304 54-55 0.2346847754128092 2304 56-57 0.5081728391247466 2304 58-59 0.33079933518363447 2304 60-61 0.3320867944791317 2304 62-63 0.327919100634098 2304 64-65 0.37533247389063007 2304 66-67 0.4066737160775702 2304 68-69 0.481531935395239 2304 70-71 0.37243440217060453 2304 72-73 0.5745264224523794 2304 74-75 0.6901987078786647 2304 76-77 0.5373996768541502 2304 78-79 0.4101901903273806 2304 80-81 0.5258348264009989 2304 82-83 0.42964966746519195 2304 84-85 0.3891723625178898 2304 86-87 0.3714432805167327 2304 88-89 0.5518272237446809 2304 90-91 0.4814888463720344 2304 92-93 0.5828473356613877 2304 94-95 0.5106539150094527 2304 96-97 0.38387021402633437 2304 98-99 0.5313306273919203 2304 100-101 0.49820384633591175 2202 1 0.16397936618086462 2202 2 -0.02649575298791973 2202 3 0.025225713444054776 2202 4 -0.07228959274635827 2202 5 -0.13086911419194536 2202 6 0.2630603362048447 2202 7 0.002377564339383298 2202 8 -0.029999873730822912 2202 9 -0.10862283010568063 2202 10-11 -0.06207193992342752 2202 12-13 0.06259309640914523 2202 14-15 -0.011744298416964227 2202 16-17 -0.0659679542438596 2202 18-19 -0.07133276998403204 2202 20-21 0.045570216689831966 2202 22-23 0.07211157326906914 2202 24-25 -0.0028690603026930717 2202 26-27 -0.047742009845109123 2202 28-29 -0.11287251910484741 2202 30-31 0.11039417269236651 2202 32-33 0.03323527245076008 2202 34-35 0.1466840818900792 2202 36-37 -0.04673085802354393 2202 38-39 0.09770644323299393 2202 40-41 0.07297312304589809 2202 42-43 0.05771664109730068 2202 44-45 -0.12890210691110582 2202 46-47 0.11595856346141176 2202 48-49 -0.12348216325448647 2202 50-51 -0.005803281482840816 2202 52-53 0.1569076871863473 2202 54-55 0.29914838877001415 2202 56-57 0.4293224196962342 2202 58-59 0.23754966521105558 2202 60-61 0.19784851567914075 2202 62-63 0.20236308159107352 2202 64-65 0.01790854944152187 2202 66-67 0.1289200529225738 2202 68-69 0.2835826849507228 2202 70-71 0.36135160361775576 2202 72-73 0.5320502013509412 2202 74-75 0.5036553144999232 2202 76-77 0.46043683505426003 2202 78-79 0.4069715518920809 2202 80-81 0.19362191043205357 2202 82-83 0.1971794495774084 2202 84-85 0.1671876084903623 2202 86-87 0.291013408605199 2202 88-89 0.2776013197283973 2202 90-91 0.1975669558475559 2202 92-93 0.3329195136034073 2202 94-95 0.24684453247432359 2202 96-97 0.5256037987712006 2202 98-99 0.2904148436191143 2202 100-101 0.42882740402151853 1102 1 0.09650331643930343 1102 2 -0.18603618533790822 1102 3 0.05330077427723978 1102 4 -0.05047673385624307 1102 5 -0.01822950598933204 1102 6 0.015674472784439786 1102 7 0.09744209501873513 1102 8 0.045136064794917274 1102 9 0.061086779697468785 1102 10-11 0.061614786502275365 1102 12-13 0.1496132246402766 1102 14-15 0.012308989142773896 1102 16-17 0.02621261696755539 1102 18-19 0.18408925783447927 1102 20-21 0.07853996314619138 1102 22-23 0.15015447361049183 1102 24-25 -0.01721899469785626 1102 26-27 0.31540001242716187 1102 28-29 0.062397239590893605 1102 30-31 0.19708927240058927 1102 32-33 0.09532770294515558 1102 34-35 0.22074030091179964 1102 36-37 0.15518760197033998 1102 38-39 0.053350213683685865 1102 40-41 0.06166281516964034 1102 42-43 -0.009415229329512442 1102 44-45 -0.048440643439690234 1102 46-47 0.13467776706235668 1102 48-49 0.013253634561074534 1102 50-51 -0.01609175018124631 1102 52-53 0.17249129633387383 1102 54-55 0.36814398430185946 1102 56-57 0.43159002323248075 1102 58-59 0.3697739002278624 1102 60-61 0.3186141579175228 1102 62-63 0.27522542527139393 1102 64-65 0.20177187826426035 1102 66-67 0.30610638862932404 1102 68-69 0.14890276208454623 1102 70-71 0.3352437249605842 1102 72-73 0.42815544383206117 1102 74-75 0.31896604091192415 1102 76-77 0.37187982903127903 1102 78-79 0.16468493450820532 1102 80-81 0.2709126874962955 1102 82-83 0.2084824822925242 1102 84-85 0.26579396578294023 1102 86-87 0.28730639303892147 1102 88-89 0.23518263677221896 1102 90-91 0.14958844496177548 1102 92-93 0.19423418657635594 1102 94-95 0.3349219128000662 1102 96-97 0.3053061419722205 1102 98-99 0.3233149411062719 1102 100-101 0.3628615921434921 2305 1 0.15443989732700558 2305 2 0.1483098028848886 2305 3 0.0671366419626871 2305 4 0.1778042233838164 2305 5 0.09976030230436095 2305 6 0.23773406001994601 2305 7 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-0.44331243491546246 1213 50-51 -0.4515347939462586 1213 52-53 -0.29157492770072224 1213 54-55 -0.6849209919017412 1213 56-57 -0.7390934199826162 1213 58-59 -1.1445284718708493 1213 60-61 -0.7716383164667349 1213 62-63 -0.8931522613702683 1213 64-65 -0.7728873940126917 1213 66-67 -0.4461812091061219 1213 68-69 -0.48460482678494543 1213 70-71 -0.565906076026863 1213 72-73 -0.3566500583790173 1213 74-75 -0.41162528016156585 1213 76-77 -0.6111537864432641 1213 78-79 -0.7649149833195885 1213 80-81 -0.7479255821300121 1213 82-83 -0.6798768431302094 1213 84-85 -0.7083547104654926 1213 86-87 -0.7547505846817089 1213 88-89 -0.6143337559455055 1213 90-91 -0.5239970162131051 1213 92-93 -0.5516511039054457 1213 94-95 -0.47283254866009017 1213 96-97 -0.4355372986257855 1213 98-99 -0.8324736112850246 1213 100-101 -0.9638048139230548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1073.0 3 135.0 4 216.0 5 204.0 6 392.0 7 2588.0 8 13196.0 9 39045.0 10 49709.0 11 35407.0 12 21768.0 13 14884.0 14 11211.0 15 9238.0 16 8238.0 17 7987.0 18 8047.0 19 8548.0 20 9268.0 21 10141.0 22 11049.0 23 11973.0 24 13625.0 25 16097.0 26 19081.0 27 22966.0 28 28199.0 29 34207.0 30 41640.0 31 54271.0 32 74445.0 33 105177.0 34 158319.0 35 262196.0 36 472161.0 37 629698.0 38 122990.0 39 79.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.413021081472486 23.43673408311848 22.169773189705733 13.980471645703298 2 31.88313262958033 25.72795956343924 29.85193153714653 12.5369762698339 3 36.26374004231078 19.851336331134696 28.254822285280547 15.630101341273983 4 30.073803275503863 17.421647413575986 32.322327596803405 20.182221714116746 5 34.587153912680165 15.892463837389037 31.943453419737317 17.57692883019348 6 31.504348076566735 16.341949233244954 36.114895671528146 16.038807018660165 7 35.91162120771225 18.68163372849964 27.81965939847782 17.587085665310283 8 29.453173115644947 30.138119635491723 23.144762804442763 17.263944444420567 9 31.832330635453605 26.6146343895218 23.43777464245179 18.115260332572806 10-11 26.723219994637294 29.164475176866116 27.54176292033634 16.570541908160248 12-13 28.37424944504226 24.398261186140637 27.370049410527315 19.857439958289785 14-15 25.593663406849355 24.6333403965335 29.19176932304599 20.58122687357115 16-17 26.989749049952565 25.46286139138301 29.964920498999188 17.582469059665236 18-19 26.509506696933155 25.586298598212377 29.969989935046236 17.934204769808233 20-21 25.07283411569532 27.940754638575406 28.869533305157276 18.116877940572003 22-23 27.895708278186042 25.502793950377 28.355217111100657 18.246280660336307 24-25 24.27641915468188 28.699780814714078 28.500035561034586 18.523764469569453 26-27 22.67082644080115 29.979092054377883 28.897506288314368 18.4525752165066 28-29 23.528432056950297 29.12221733846989 28.287332695572736 19.062017909007075 30-31 25.745994400000516 27.115772256362703 28.152871338753506 18.985362004883275 32-33 22.661550664304198 26.96333512512486 29.771849993910205 20.603264216660737 34-35 20.398570468105838 26.84833670357142 33.927321655006416 18.825771173316323 36-37 21.57214459262575 27.631700708673453 30.179051414872028 20.617103283828772 38-39 20.308911407684967 27.25363866444473 28.086520335150173 24.35092959272013 40-41 22.272223333065728 26.48152145909306 29.445586735940253 21.800668471900956 42-43 24.200285082935274 26.59874609163097 30.54578156720672 18.655187258227038 44-45 21.804643009807496 28.705555141821915 30.900586693436427 18.58921515493416 46-47 23.435321857301034 29.268381950061457 28.855280956255168 18.441015236382338 48-49 20.665958360648325 28.844589263243332 30.57836738692131 19.91108498918703 50-51 19.766976479126466 27.55811647860546 30.987654507042528 21.68725253522555 52-53 24.6841709904297 26.874202986168477 28.108109039135304 20.33351698426652 54-55 26.216595120080093 26.851484526198576 28.1279146632869 18.804005690434426 56-57 26.571311275483307 26.765965957173815 27.304678493628515 19.358044273714363 58-59 24.685600765877545 26.776952803678867 27.629372618069382 20.90807381237421 60-61 20.988811270690455 27.08006536229064 27.435115529054023 24.496007837964882 62-63 23.674222246614733 27.187496973355845 27.726427957260018 21.411852822769404 64-65 21.322106738604347 28.579149709860772 29.884178926113563 20.214564625421314 66-67 20.71301237796066 28.38872125975981 28.500584824524662 22.397681537754867 68-69 20.812724118485228 26.868884497996113 32.94641819379334 19.37197318972531 70-71 20.296847647974626 31.271495632283337 29.688014090310975 18.74364262943106 72-73 20.095300308286557 34.326464447210014 26.84653491762673 18.7317003268767 74-75 20.821217669656768 33.77934899158013 26.463342285636944 18.93609105312616 76-77 20.83220545915489 33.05124644262388 26.714286267592147 19.40226183062909 78-79 20.729203760703253 32.733696464900206 27.25246824391855 19.284631530477984 80-81 19.823834901545432 32.80000636953914 27.464032546623518 19.912126182291907 82-83 19.27932396364931 32.34594723062158 27.81896730254969 20.555761503179415 84-85 19.439047268639133 31.682812983391102 28.149209921636952 20.728929826332816 86-87 19.38159411791551 31.70386022558094 28.904075146574222 20.01047050992933 88-89 19.80449045907154 31.856046800354697 28.692428762997856 19.647033977575905 90-91 20.572858431465306 31.161770811429275 27.841364863268097 20.424005893837318 92-93 19.963915953955826 31.777223194070753 27.635455587980793 20.623405263992627 94-95 19.812320458524535 31.838993132588993 27.84032973556476 20.50835667332171 96-97 20.259432937186048 32.398563731200504 27.275917581511997 20.06608575010145 98-99 20.739914174173915 31.456736294338683 27.17172912783916 20.631620403648242 100-101 20.78993627206654 31.522235048727808 26.735408989056147 20.9524196901495 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 911.0 1 523.5 2 111.0 3 101.5 4 99.5 5 93.5 6 147.5 7 233.5 8 329.5 9 444.5 10 579.0 11 685.5 12 733.0 13 700.5 14 646.0 15 624.5 16 555.5 17 477.5 18 461.5 19 499.5 20 560.5 21 620.0 22 700.5 23 871.5 24 1109.0 25 1389.0 26 1905.5 27 2673.5 28 3418.5 29 4218.5 30 5424.0 31 7124.5 32 11096.5 33 19260.5 34 33399.5 35 53770.5 36 81555.0 37 115846.0 38 144680.0 39 160253.0 40 164284.5 41 152047.5 42 141444.5 43 144932.5 44 141759.0 45 132292.0 46 127021.0 47 114782.0 48 94602.5 49 79673.5 50 69170.5 51 59046.5 52 49133.0 53 41896.0 54 34933.0 55 26854.0 56 20981.5 57 16871.5 58 13544.5 59 10674.5 60 8218.5 61 6391.0 62 4927.5 63 3744.0 64 2874.5 65 2184.0 66 1656.0 67 1226.5 68 962.0 69 766.5 70 537.0 71 404.5 72 287.0 73 190.0 74 153.0 75 121.0 76 88.0 77 67.0 78 56.5 79 42.5 80 36.0 81 31.5 82 26.0 83 24.5 84 21.0 85 16.0 86 15.0 87 15.0 88 14.5 89 15.0 90 8.5 91 5.0 92 5.0 93 3.5 94 4.5 95 4.0 96 1.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16415765316372666 2 0.15677399303188538 3 0.12337580941227783 4 0.11770927954365545 5 0.13015847395199245 6 0.15012011326191216 7 0.1260802895768476 8 0.11663607312914365 9 0.11071197372103844 10-11 0.09804813802979909 12-13 0.12618761021829877 14-15 0.12256017253724884 16-17 0.11206421380332333 18-19 0.11084075849077987 20-21 0.1044659123885797 22-23 0.11781660018510665 24-25 0.4083765048500344 26-27 0.29646253994474275 28-29 0.3798292142240203 30-31 0.2147915318003939 32-33 0.2536630681340117 34-35 0.2657259082331245 36-37 0.2624204324764281 38-39 0.28199571747712354 40-41 0.2751057322959577 42-43 0.330010972462382 44-45 0.27684432668746684 46-47 0.301935892658753 48-49 0.3015924666061092 50-51 0.3022793187113968 52-53 0.3059711487773174 54-55 0.2842923792041788 56-57 0.29592593673748685 58-59 0.29635521930329156 60-61 0.2754276942203112 62-63 0.2726158934142903 64-65 0.28038590785535583 66-67 0.28113715234551406 68-69 0.27808924612830055 70-71 0.2526542541043706 72-73 0.2428022192191522 74-75 0.25145226292011735 76-77 0.2479106817522284 78-79 0.2447125266369832 80-81 0.2536630681340117 82-83 0.29496005096442623 84-85 0.3008626862442412 86-87 0.2899374449445109 88-89 0.2971279279217401 90-91 0.27317396074983646 92-93 0.25771978838086634 94-95 0.24325296591324713 96-97 0.24336028655469835 98-99 0.2643522040225494 100-101 0.2641804909962275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2329468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.75316352555086 #Duplication Level Percentage of deduplicated Percentage of total 1 87.51926277681041 37.417253531326324 2 5.444523006942607 4.655411648688823 3 1.6746920131061074 2.147951444737781 4 1.1695475923123198 2.000074378601716 5 0.7438945737590886 1.5901923178846138 6 0.4552279892250228 1.1677461998847065 7 0.358089653024463 1.0716625844793846 8 0.2306603631604448 0.7889168180049156 9 0.22256081272523798 0.8563660938739441 >10 1.6890746312182146 14.644783285498722 >50 0.24841417096256724 7.4758544941899165 >100 0.2263226307254586 19.277965230117307 >500 0.013865109861566352 3.9024934617593052 >1k 0.003763486448310962 2.706682308935936 >5k 1.0118971832934121E-4 0.2966462020166935 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 6857 0.2943590553722996 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3433 0.14737270484076193 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3249 0.139473905629955 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3219 0.13818605793254082 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2744 0.11779513605681641 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2696 0.11573457974095372 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2650 0.11375987993825198 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2553 0.10959583904994617 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2435 0.1045303047734504 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005065534276495749 0.0 0.0 2 0.005494816842300474 0.0 0.0 3 0.006353381973909923 0.0 0.0 4 0.006739736283134175 0.0 0.0 5 0.007984655723967875 0.0 0.0 6 0.009487144704284411 0.0 0.0 7 0.010732064145118113 0.0 0.0 8 0.011891127072790868 0.0 0.0 9 0.01382289861891213 0.0 0.0 10-11 0.020519706645465832 0.0 0.0 12-13 0.0267228397213441 0.0 0.0 14-15 0.03320500646499544 0.0 0.0 16-17 0.039107641744810406 0.0 0.0 18-19 0.04546102371872032 0.0 0.0 20-21 0.05175001330775954 0.0 0.0 22-23 0.05863999848892537 0.0 0.0 24-25 0.06527241413060836 0.0 0.0 26-27 0.07164726023280851 0.0 0.0 28-29 0.08016851916403231 0.0 0.0 30-31 0.13749920582725325 0.0 0.0 32-33 0.14934740464346366 0.0 0.0 34-35 0.1593067601701333 0.0 0.0 36-37 0.16956661349286617 0.0 0.0 38-39 0.18152213295052777 0.0 0.0 40-41 0.1964182379839517 0.0 0.0 42-43 0.21408321556681612 0.0 0.0 44-45 0.23194137030429265 0.0 0.0 46-47 0.2514737270484076 0.0 2.1464128290236226E-5 48-49 0.27459059321699203 0.0 4.292825658047245E-5 50-51 0.29963923093169775 0.0 4.292825658047245E-5 52-53 0.32790748788993884 0.0 4.292825658047245E-5 54-55 0.35945975647658607 0.0 4.292825658047245E-5 56-57 0.3940384671521566 0.0 8.58565131609449E-5 58-59 0.4317080123015212 0.0 8.58565131609449E-5 60-61 0.47025758671078544 0.0 8.58565131609449E-5 62-63 0.5118121390806828 0.0 1.0732064145118113E-4 64-65 0.5563716694112133 0.0 1.2878476974141737E-4 66-67 0.6030132201858964 0.0 1.2878476974141737E-4 68-69 0.6534753857962419 0.0 1.2878476974141737E-4 70-71 0.7082947694495052 0.0 1.2878476974141737E-4 72-73 0.7659903462936601 0.0 1.2878476974141737E-4 74-75 0.8268196858681897 0.0 1.2878476974141737E-4 76-77 0.8928648086172465 0.0 1.2878476974141737E-4 78-79 0.9647267101329574 0.0 1.2878476974141737E-4 80-81 1.0404306906126206 0.0 1.2878476974141737E-4 82-83 1.1214792390365527 0.0 1.2878476974141737E-4 84-85 1.20939630851336 0.0 1.5024889803165359E-4 86-87 1.3066502737964205 0.0 1.717130263218898E-4 88-89 1.4118674306751584 4.292825658047245E-5 1.717130263218898E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1760 0.0 86.76679 9 CGGGAGA 2540 0.0 85.71089 4 AGAGCGG 1995 0.0 81.29881 8 TCGGGAG 2690 0.0 78.991936 3 AAGCGGC 880 0.0 78.144005 9 TCGGAGA 420 0.0 77.92142 4 GAGAGCG 2460 0.0 77.11435 7 GAACCGG 360 0.0 76.428825 1 AAGCGTC 350 0.0 75.88052 9 CGGAAAG 1000 0.0 73.99147 5 ATTCGGA 260 0.0 72.976166 2 ACCGGAA 390 0.0 71.75372 3 TCGGGAA 995 0.0 70.07309 3 GGATCGG 705 0.0 69.98032 1 ATCGGGA 3820 0.0 69.91002 2 GATCGGG 4055 0.0 68.90586 1 AACCGGA 395 0.0 68.44979 2 GTTCGGA 175 0.0 67.76357 2 GGAAAGC 1220 0.0 63.37016 6 GGGAGAG 5015 0.0 61.1914 5 >>END_MODULE