FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329468
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT68570.2943590553722996Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA34330.14737270484076193No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32490.139473905629955No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC32190.13818605793254082No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG27440.11779513605681641No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26960.11573457974095372No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC26500.11375987993825198No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT25530.10959583904994617No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT24350.1045303047734504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC17600.086.766799
CGGGAGA25400.085.710894
AGAGCGG19950.081.298818
TCGGGAG26900.078.9919363
AAGCGGC8800.078.1440059
TCGGAGA4200.077.921424
GAGAGCG24600.077.114357
GAACCGG3600.076.4288251
AAGCGTC3500.075.880529
CGGAAAG10000.073.991475
ATTCGGA2600.072.9761662
ACCGGAA3900.071.753723
TCGGGAA9950.070.073093
GGATCGG7050.069.980321
ATCGGGA38200.069.910022
GATCGGG40550.068.905861
AACCGGA3950.068.449792
GTTCGGA1750.067.763572
GGAAAGC12200.063.370166
GGGAGAG50150.061.19145
Produced by FastQC (version 0.11.2)