FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403765
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT325570.6024873398454595Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC89260.16518112834292387No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG88920.16455193739920224No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG81400.15063571417335875No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC80500.14897020873409558No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA54350.10057802291550427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC60100.090.3496869
CGGGAGA80950.089.938894
AGAGCGG63450.088.7908868
GAGAGCG72300.088.023337
ATTCGGA14400.087.951862
CGGAGAG25500.087.048585
CGGAAAG30800.085.7749565
AAGCGGC19850.084.096479
GTCCGGA4300.083.83812
CCGGAGA11500.083.7248154
TCGGGAG87800.083.408213
GTTCGGA10350.082.953452
TCGGAGA18250.080.046784
AAGCGTC8800.079.758089
TTCGGAG13100.079.653963
AAGGGGC49550.078.864079
GATCCGG16000.077.3814541
TCGGGAA25250.077.203413
GGAAAGC24100.077.148066
GATCCGA3350.076.465471
Produced by FastQC (version 0.11.2)