##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34042-1.34200000035847.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5403765 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.980933663843636 33.0 33.0 33.0 27.0 33.0 2 30.456854248843168 33.0 33.0 33.0 22.0 33.0 3 30.272963017451723 33.0 33.0 33.0 22.0 33.0 4 33.568954793555974 37.0 37.0 37.0 15.0 37.0 5 33.6692141497641 37.0 37.0 37.0 22.0 37.0 6 34.4073363664038 37.0 37.0 37.0 27.0 37.0 7 33.57839524849804 37.0 37.0 37.0 22.0 37.0 8 34.07343713133343 37.0 37.0 37.0 27.0 37.0 9 33.34310633419477 37.0 37.0 37.0 15.0 37.0 10-11 33.41125594839894 37.0 37.0 37.0 15.0 37.0 12-13 33.37045633923755 37.0 37.0 37.0 15.0 37.0 14-15 34.84953833114504 40.0 37.0 40.0 15.0 40.0 16-17 34.77264074214922 40.0 37.0 40.0 15.0 40.0 18-19 34.870784813921404 40.0 37.0 40.0 15.0 40.0 20-21 34.85404546644793 40.0 37.0 40.0 15.0 40.0 22-23 35.78162540376941 40.0 37.0 40.0 27.0 40.0 24-25 34.94063759249339 40.0 37.0 40.0 18.5 40.0 26-27 34.86900429607876 37.0 37.0 40.0 18.5 40.0 28-29 34.45580072412476 37.0 37.0 40.0 15.0 40.0 30-31 34.43845504014331 37.0 37.0 40.0 15.0 40.0 32-33 34.2213908265811 37.0 37.0 40.0 15.0 40.0 34-35 34.551175615519924 37.0 37.0 40.0 18.5 40.0 36-37 34.199177702953406 37.0 37.0 40.0 15.0 40.0 38-39 34.16759055954506 37.0 37.0 40.0 15.0 40.0 40-41 33.95881547402598 37.0 35.0 40.0 15.0 40.0 42-43 33.825681353648804 37.0 33.0 40.0 15.0 40.0 44-45 33.6844043921229 37.0 33.0 40.0 15.0 40.0 46-47 33.59417711540009 37.0 33.0 40.0 15.0 40.0 48-49 33.47818021324021 37.0 33.0 40.0 15.0 40.0 50-51 32.561346857237496 37.0 33.0 38.5 15.0 38.5 52-53 33.02998927599553 37.0 33.0 38.5 15.0 40.0 54-55 33.56483803422243 37.0 33.0 40.0 15.0 40.0 56-57 33.924304535819005 37.0 33.0 40.0 18.5 40.0 58-59 33.25935953913614 37.0 33.0 40.0 15.0 40.0 60-61 33.119640102780195 37.0 33.0 40.0 10.5 40.0 62-63 32.68998096697395 37.0 33.0 40.0 6.0 40.0 64-65 32.45903161962077 37.0 33.0 37.0 6.0 40.0 66-67 32.33203673734887 37.0 33.0 37.0 10.5 40.0 68-69 32.49698737824461 37.0 33.0 37.0 15.0 40.0 70-71 32.04508430696005 37.0 33.0 37.0 15.0 38.5 72-73 32.45331690034634 37.0 33.0 37.0 18.5 37.0 74-75 32.58226301476841 37.0 33.0 37.0 22.0 37.0 76-77 32.26006756400399 37.0 33.0 37.0 22.0 37.0 78-79 31.765518763306694 37.0 33.0 37.0 22.0 37.0 80-81 31.204550993612788 33.0 33.0 37.0 15.0 37.0 82-83 30.824029819949608 33.0 33.0 37.0 15.0 37.0 84-85 30.577432493826066 33.0 27.0 37.0 15.0 37.0 86-87 30.424423619457915 33.0 27.0 37.0 15.0 37.0 88-89 30.293139320455275 33.0 27.0 37.0 15.0 37.0 90-91 30.160384380149765 33.0 27.0 37.0 10.5 37.0 92-93 29.995420415210507 33.0 27.0 37.0 6.0 37.0 94-95 29.85391333634975 33.0 27.0 37.0 6.0 37.0 96-97 29.729511183406384 33.0 27.0 37.0 6.0 37.0 98-99 29.576730390755337 33.0 27.0 37.0 6.0 37.0 100-101 28.378642576055768 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.03111843534600567 2306 2 -0.19324398871587078 2306 3 -0.19035201658534362 2306 4 -0.04038082897986328 2306 5 -0.049716208705554266 2306 6 -0.31222852309719684 2306 7 0.3338336179536654 2306 8 -0.2814491056841675 2306 9 0.06882296261876064 2306 10-11 0.004863882764361449 2306 12-13 -0.006349962106007467 2306 14-15 0.2846048272608286 2306 16-17 0.2562086283872418 2306 18-19 0.2178228903353414 2306 20-21 0.20904677208957878 2306 22-23 0.09142499694942785 2306 24-25 0.4935667421006471 2306 26-27 0.07865246589423691 2306 28-29 0.22876488906216963 2306 30-31 0.35790911261523917 2306 32-33 0.3980439862109648 2306 34-35 0.21827057277828743 2306 36-37 0.46390283554485023 2306 38-39 0.2345730183575867 2306 40-41 0.21122376194556125 2306 42-43 0.19476748164012037 2306 44-45 0.38186099492201464 2306 46-47 0.19835170332114416 2306 48-49 0.3592129872552903 2306 50-51 0.36389760063359944 2306 52-53 0.30109573562170056 2306 54-55 0.47695390830286044 2306 56-57 0.3834215562846026 2306 58-59 0.6586022075297393 2306 60-61 0.5502588997763453 2306 62-63 0.38717932279777045 2306 64-65 0.4102043445645478 2306 66-67 0.3133981527405183 2306 68-69 0.19189604599542776 2306 70-71 0.3796642408016524 2306 72-73 3.7343219450036713E-4 2306 74-75 0.28848317585311634 2306 76-77 0.39986484526989585 2306 78-79 0.5220937691078937 2306 80-81 0.6734218875873701 2306 82-83 0.5148311225348969 2306 84-85 0.48698899102076965 2306 86-87 0.622821734518233 2306 88-89 0.48652607796315905 2306 90-91 0.5213343434566227 2306 92-93 0.3590137304359722 2306 94-95 0.5213277807884786 2306 96-97 0.5461552715367262 2306 98-99 0.5101450207123648 2306 100-101 0.6216044049181981 2307 1 -0.08230623101558265 2307 2 -0.2546179262548627 2307 3 -0.33690446057057954 2307 4 -0.12834309699296398 2307 5 -0.2396338761000223 2307 6 -0.28489232729245373 2307 7 0.25916121009860404 2307 8 -0.3839580732449903 2307 9 -0.13006697681771584 2307 10-11 -0.2301582193488585 2307 12-13 -0.18712006763730216 2307 14-15 0.07708755008521706 2307 16-17 -0.013680836678261699 2307 18-19 -0.017651082619018155 2307 20-21 0.010018014161737199 2307 22-23 -0.10626350052925204 2307 24-25 0.18100935458505063 2307 26-27 -0.04905819055721139 2307 28-29 0.05439217156632736 2307 30-31 0.09981345213488879 2307 32-33 0.008222248973495994 2307 34-35 -0.049220069766938934 2307 36-37 0.3098398563002007 2307 38-39 -0.07174127353002291 2307 40-41 -0.061168619201772856 2307 42-43 0.1521441126964831 2307 44-45 0.10977419315709369 2307 46-47 -0.08369108104151479 2307 48-49 -0.00833784516828473 2307 50-51 0.0367940009377179 2307 52-53 -9.573403166456274E-4 2307 54-55 0.09375586004698278 2307 56-57 0.18096867659288307 2307 58-59 0.2032746722443335 2307 60-61 -0.04004947384603241 2307 62-63 0.11093373347306823 2307 64-65 0.11393437062245937 2307 66-67 0.15525525823282038 2307 68-69 0.03219089517144624 2307 70-71 0.061276760946171294 2307 72-73 -0.23792414461460254 2307 74-75 0.08898873834012022 2307 76-77 0.26708556748344847 2307 78-79 0.2356507878487939 2307 80-81 0.23410469748807472 2307 82-83 0.19397094337862697 2307 84-85 0.2902584142376554 2307 86-87 0.3276443165259266 2307 88-89 0.32469925423421486 2307 90-91 0.25182173526105345 2307 92-93 0.2948079444097722 2307 94-95 0.3503323977826547 2307 96-97 0.352525488837621 2307 98-99 0.30426899399163077 2307 100-101 0.3821154584116506 2201 1 0.051913769153930645 2201 2 -0.062363271265322595 2201 3 -0.020905726480727083 2201 4 0.06703138174991352 2201 5 0.023713272665915497 2201 6 0.016254059990082226 2201 7 0.40692057638071333 2201 8 0.08296782954086268 2201 9 0.12475132178936121 2201 10-11 0.11607096820441143 2201 12-13 0.09118249881238683 2201 14-15 0.32834555207822547 2201 16-17 0.36256721390486746 2201 18-19 0.22646188275885493 2201 20-21 0.2691674841867311 2201 22-23 0.2698991117135634 2201 24-25 0.5143866179064673 2201 26-27 0.3025505475525634 2201 28-29 0.2714400264207413 2201 30-31 0.3762863213374956 2201 32-33 0.18723343148250393 2201 34-35 0.11760400323085918 2201 36-37 0.3842756075764058 2201 38-39 0.20403079695653759 2201 40-41 0.17840196270522313 2201 42-43 0.26601260939336413 2201 44-45 0.37440673867173757 2201 46-47 0.3209582176527448 2201 48-49 0.36760243063182685 2201 50-51 0.4242647823875032 2201 52-53 0.3312512566722958 2201 54-55 0.5424184283976601 2201 56-57 0.5857586950336398 2201 58-59 0.6207315111256335 2201 60-61 0.4417548083326679 2201 62-63 0.46264913707501165 2201 64-65 0.47956547768541213 2201 66-67 0.3838768676399056 2201 68-69 0.3940718720714642 2201 70-71 0.40759407334203956 2201 72-73 0.27034748240988193 2201 74-75 0.4453457746452827 2201 76-77 0.4830584808809064 2201 78-79 0.583291675596282 2201 80-81 0.542903945993924 2201 82-83 0.4322051606695929 2201 84-85 0.44161050435156923 2201 86-87 0.41819601663538464 2201 88-89 0.41616402457837864 2201 90-91 0.4994587105531032 2201 92-93 0.43841639979355307 2201 94-95 0.4137822087739771 2201 96-97 0.5918951128060073 2201 98-99 0.4679258699845015 2201 100-101 0.5731678468711046 1101 1 0.228468892318606 1101 2 0.08008980718769365 1101 3 0.17927786008000268 1101 4 0.16874113064670837 1101 5 0.20944271923570312 1101 6 0.22646331335885606 1101 7 0.3607716193667443 1101 8 0.2418393657972615 1101 9 0.25866768077546 1101 10-11 0.2657934474932304 1101 12-13 0.2911803231879162 1101 14-15 0.40394996210378764 1101 16-17 0.38241811898549827 1101 18-19 0.413340622069974 1101 20-21 0.357916384731773 1101 22-23 0.31582244326330056 1101 24-25 0.4763856525686876 1101 26-27 0.4511453165728412 1101 28-29 0.4518874321241029 1101 30-31 0.4712635898847637 1101 32-33 0.47764426584213027 1101 34-35 0.26873500821505303 1101 36-37 0.5552825407980748 1101 38-39 0.39564631559552055 1101 40-41 0.48047261503251093 1101 42-43 0.37737573613134856 1101 44-45 0.34749933811480815 1101 46-47 0.33157901854112737 1101 48-49 0.3390871532299684 1101 50-51 0.2223655456354976 1101 52-53 0.30280593984646487 1101 54-55 0.5661539005436182 1101 56-57 0.5815164010885567 1101 58-59 0.5990088739128936 1101 60-61 0.5267463428596884 1101 62-63 0.6180220884604424 1101 64-65 0.599356438403646 1101 66-67 0.5161109406438555 1101 68-69 0.49241826540740874 1101 70-71 0.5882725609480062 1101 72-73 0.5632102038422246 1101 74-75 0.5433139012602339 1101 76-77 0.6229583284759812 1101 78-79 0.6446808125156274 1101 80-81 0.5385418536854445 1101 82-83 0.5540999780540723 1101 84-85 0.662153035585682 1101 86-87 0.6636763479475398 1101 88-89 0.6882328502152149 1101 90-91 0.6327153618124548 1101 92-93 0.5192361222672837 1101 94-95 0.48857180225472163 1101 96-97 0.4450066798019634 1101 98-99 0.5404762732257211 1101 100-101 0.5735257127683902 2304 1 0.3077972539320619 2304 2 0.22592883187902046 2304 3 0.25710410571588227 2304 4 0.24573453716934068 2304 5 0.253289880444477 2304 6 0.3421805854786726 2304 7 0.2537430046947122 2304 8 0.24346573004596195 2304 9 0.1985599631598518 2304 10-11 0.29321924068292304 2304 12-13 0.29292738000668095 2304 14-15 0.3648147086024238 2304 16-17 0.3920210612274957 2304 18-19 0.46461141621232827 2304 20-21 0.3600072040458073 2304 22-23 0.43155642046924925 2304 24-25 0.4772306347365074 2304 26-27 0.46724083477936773 2304 28-29 0.4717250238008077 2304 30-31 0.42048999009301014 2304 32-33 0.3462244811163515 2304 34-35 0.450620992312345 2304 36-37 0.26948233362817575 2304 38-39 0.35129055495726647 2304 40-41 0.36686098882926643 2304 42-43 0.37457364242721525 2304 44-45 0.34548365814400483 2304 46-47 0.3500551792427089 2304 48-49 0.3836185520830213 2304 50-51 0.17047525444370137 2304 52-53 0.29440643469270356 2304 54-55 0.5711745504452637 2304 56-57 0.6397656275558603 2304 58-59 0.5456069401482253 2304 60-61 0.4462485581285378 2304 62-63 0.5771636617172717 2304 64-65 0.6119451632084747 2304 66-67 0.53669677258398 2304 68-69 0.5658251100648641 2304 70-71 0.5315603365819257 2304 72-73 0.6521127266456759 2304 74-75 0.6671254249519336 2304 76-77 0.6549253557220496 2304 78-79 0.6237663801262805 2304 80-81 0.46823296389607805 2304 82-83 0.4750674105158801 2304 84-85 0.5468888134523162 2304 86-87 0.5152299209574878 2304 88-89 0.5954711841612905 2304 90-91 0.5448905518747402 2304 92-93 0.4559826826296671 2304 94-95 0.5319689324685193 2304 96-97 0.47955662197150417 2304 98-99 0.46850445584653144 2304 100-101 0.6188505696681936 2202 1 0.03408154652190376 2202 2 -0.1279966619403119 2202 3 -0.07864624000294285 2202 4 -0.08596591634129425 2202 5 -0.19885568982551405 2202 6 -0.04635664647601345 2202 7 -0.03882117219254866 2202 8 -0.08925497141602534 2202 9 -0.26503116469874755 2202 10-11 -0.19301012941436113 2202 12-13 -0.1526819909360313 2202 14-15 -0.08933482309198837 2202 16-17 -0.09847574728956943 2202 18-19 -0.19701319051364408 2202 20-21 -0.07686106651465252 2202 22-23 -0.02298095037684078 2202 24-25 3.5771433336151404E-4 2202 26-27 -0.21107217813753465 2202 28-29 -0.12450316873890444 2202 30-31 -0.02919191839567503 2202 32-33 -0.0669243078109858 2202 34-35 -0.02839085572838229 2202 36-37 -0.006525232934023961 2202 38-39 -0.01929843518144736 2202 40-41 -0.00786832389982095 2202 42-43 0.04818714387953804 2202 44-45 -0.0835565720533964 2202 46-47 -0.01249833923890975 2202 48-49 0.01455314027001009 2202 50-51 -0.011605942679430825 2202 52-53 -2.1906400434090756E-5 2202 54-55 0.1826448669365064 2202 56-57 0.25349655263300264 2202 58-59 0.19290006743918298 2202 60-61 0.1985250184508871 2202 62-63 0.20905871416387356 2202 64-65 0.15841203760837175 2202 66-67 0.19810322922268853 2202 68-69 0.259355488030657 2202 70-71 0.2814250684309272 2202 72-73 0.28097511545707476 2202 74-75 0.2719008737825277 2202 76-77 0.3426354289721658 2202 78-79 0.3381562845327615 2202 80-81 0.18902606401262645 2202 82-83 0.27647066661350905 2202 84-85 0.11055919999313346 2202 86-87 0.17271873416062533 2202 88-89 0.1900975964755247 2202 90-91 0.08589567274045606 2202 92-93 0.14073859608220474 2202 94-95 0.14688345386685242 2202 96-97 0.17156538420753265 2202 98-99 0.1589158159557087 2202 100-101 0.11461347600103622 1102 1 0.11601165576172079 1102 2 0.03469706399445016 1102 3 0.04133059315380194 1102 4 -0.04424830978739891 1102 5 -0.09031977010430836 1102 6 0.089003909133055 1102 7 0.011375548390127221 1102 8 0.2022160243383695 1102 9 0.023799601264272496 1102 10-11 0.06545737649467043 1102 12-13 0.01862471287661549 1102 14-15 -0.07923946362309664 1102 16-17 0.02819884747591317 1102 18-19 0.07394749253776212 1102 20-21 -0.014852096154875483 1102 22-23 0.09152956979040994 1102 24-25 0.12442196404130357 1102 26-27 0.26305739811669326 1102 28-29 0.052050034731671246 1102 30-31 0.04968463789506217 1102 32-33 -0.05398965164595637 1102 34-35 0.11636956008928934 1102 36-37 -0.009408492296458348 1102 38-39 0.0020419815747487746 1102 40-41 -0.0019260780003307332 1102 42-43 0.03970840032265954 1102 44-45 0.014132547680645757 1102 46-47 0.012169656186998168 1102 48-49 -0.026399441795696532 1102 50-51 0.03572184345453877 1102 52-53 0.04343667113563754 1102 54-55 0.29710031028023565 1102 56-57 0.4482311589078094 1102 58-59 0.3071392786797844 1102 60-61 0.21632742849413233 1102 62-63 0.25666213139674454 1102 64-65 0.304575801010202 1102 66-67 0.24955510862666586 1102 68-69 0.22891467585743897 1102 70-71 0.21443868450842274 1102 72-73 0.36844427367079646 1102 74-75 0.4553985731519816 1102 76-77 0.4010656407092057 1102 78-79 0.2569645258038271 1102 80-81 0.2242743666338356 1102 82-83 0.07848652775076559 1102 84-85 0.20227828843465545 1102 86-87 0.2594699178183717 1102 88-89 0.3112794512554764 1102 90-91 0.2536870921171257 1102 92-93 0.20727448616566235 1102 94-95 0.27911401582333895 1102 96-97 0.28313213460085507 1102 98-99 0.16946525049208816 1102 100-101 0.09697259170929584 2305 1 0.12852512930770033 2305 2 0.16151368871510385 2305 3 0.10930530153487794 2305 4 0.06286249478608852 2305 5 0.16775964333670856 2305 6 0.27488201909078214 2305 7 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-0.22476519726306066 1213 52-53 -0.06181461608966288 1213 54-55 -0.42707000116352134 1213 56-57 -0.5890106370548338 1213 58-59 -0.9314458562967118 1213 60-61 -0.4599908642727115 1213 62-63 -0.6371529635462352 1213 64-65 -0.3542889765791273 1213 66-67 -0.2272310176752228 1213 68-69 -0.12417646111061487 1213 70-71 -0.2958898311750815 1213 72-73 -0.1319622345079381 1213 74-75 -0.4030278698154319 1213 76-77 -0.7017314179000245 1213 78-79 -0.6550960517069733 1213 80-81 -0.6498056771237266 1213 82-83 -0.5230028890714102 1213 84-85 -0.4860958508852171 1213 86-87 -0.6638411555575203 1213 88-89 -0.5131974363713745 1213 90-91 -0.4653514710075122 1213 92-93 -0.2749285867811544 1213 94-95 -0.382037787708839 1213 96-97 -0.44278827702789414 1213 98-99 -0.6020421895082322 1213 100-101 -0.9401390902650952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2665.0 3 332.0 4 493.0 5 503.0 6 1032.0 7 6130.0 8 27766.0 9 96316.0 10 146188.0 11 107886.0 12 66599.0 13 43487.0 14 31187.0 15 24112.0 16 21137.0 17 19567.0 18 18856.0 19 19379.0 20 19947.0 21 21176.0 22 22240.0 23 23718.0 24 26581.0 25 30297.0 26 35940.0 27 43646.0 28 55164.0 29 68945.0 30 81810.0 31 105150.0 32 144557.0 33 208215.0 34 317064.0 35 535962.0 36 1047180.0 37 1639843.0 38 342636.0 39 59.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.60642461140103 23.850506901268584 22.46610076332255 14.076967724007835 2 30.216292474833967 27.46048978742958 29.606917385049613 12.716300352686838 3 34.32826832429251 20.00315381773157 29.66384231356064 16.004735544415276 4 27.371623252924294 17.5833485116678 33.34164667542594 21.703381559981967 5 34.54815709262126 16.51167031363968 30.91938188254968 18.020790711189388 6 31.38778388080815 16.862676626752922 35.59830024611067 16.151239246328267 7 35.011167462648345 20.035148831247625 27.66719645635739 17.286487249746642 8 27.054758922034992 31.973669557992697 23.847871768983463 17.123699750988848 9 32.51813020695727 26.651394963376447 23.107115978853308 17.72335885081297 10-11 26.80911121195036 29.933204341842828 26.47928721410522 16.778397232101597 12-13 28.963769345282074 23.869770775669917 26.16830526281361 20.998154616234395 14-15 25.24091708405368 24.213867069878276 28.54081313106045 22.004402715007586 16-17 27.404499265920556 25.238121883126848 29.56931103253591 17.788067818416685 18-19 26.907324366800577 25.424979283594507 29.842953666120557 17.824742683484356 20-21 25.226646005936438 28.653090647564383 28.277012371705286 17.84325097479389 22-23 29.079643735210524 25.665717435084733 27.303405949641995 17.951232880062744 24-25 24.350085326491726 30.18794129686046 27.21144290280121 18.250530473846606 26-27 22.82620468835983 31.752175848809124 26.87314935790266 18.548470104928384 28-29 24.150060195747653 30.04196981294729 26.784859654729082 19.023110336575975 30-31 26.943149572972168 26.607366056151744 27.552397085152762 18.89708728572332 32-33 22.47986328744705 29.37423576977814 29.588266619166102 18.557634323608706 34-35 27.878806604752214 26.691633606904492 27.73490431690162 17.694655471441674 36-37 23.665413035034945 27.09672163606629 29.866705066751386 19.371160262147384 38-39 20.250532750749674 29.927882953432995 31.274239196293653 18.547345099523678 40-41 22.076504010065598 30.888839955284215 28.035039554096702 18.999616480553485 42-43 25.113730453579276 27.04561158007109 29.75414093236039 18.086517033989246 44-45 22.13412025630675 29.545533423346924 30.09101580805761 18.229330512288712 46-47 24.138881329136836 30.3467754181863 27.226570781370608 18.287772471306255 48-49 20.907553793028807 29.264629195811693 29.74298231332626 20.084834697833244 50-51 19.7602943996602 26.926911159374097 31.524863780008914 21.787930660956793 52-53 26.095143609124317 26.462959184677487 27.29835769997425 20.143539506223952 54-55 27.62479130685757 26.584355647452202 27.168427314463795 18.622425731226432 56-57 28.129962120354286 26.110317646242052 26.800406232562384 18.959314000841275 58-59 25.935050346205283 25.95695473004286 27.25237627758943 20.85561864616243 60-61 21.42179802767103 26.512126455623342 26.4421123312365 25.623963185469133 62-63 24.867862290236026 26.65565580947524 26.922909895073012 21.553572005215727 64-65 21.731694275181145 28.441889414615108 29.996263844238868 19.830152465964883 66-67 20.438853052834922 28.25649320127534 28.218370639093944 23.08628310679579 68-69 19.975623786722423 26.41404169364386 34.17735708878897 19.432977430844744 70-71 19.549644362325523 31.472366319978534 30.351941580940924 18.626047736755016 72-73 19.430754204343366 35.916216194404015 26.287935704536824 18.36509389671579 74-75 20.328216645399962 35.51369155777106 25.629758220120685 18.52833357670829 76-77 20.76205328111041 34.489588039441784 26.090813707218285 18.657544972229516 78-79 20.43996314932455 34.15106940151791 26.845221689874904 18.56374575928264 80-81 19.724517425848138 34.46925888864005 26.60298153089623 19.203242154615587 82-83 19.020726205645623 34.01625585848439 27.049345337543464 19.913672598326524 84-85 19.257216836264917 33.00514512382752 27.460631894569886 20.27700614533768 86-87 19.23493311987378 32.724166173621505 28.101680993979052 19.93921971252567 88-89 19.691133057871642 33.05088269575505 27.625862782358595 19.632121464014716 90-91 20.279197391015074 32.03515392834756 27.3991855505514 20.28646313008596 92-93 19.964170515613166 32.57759080906646 26.83659739954653 20.62164127577384 94-95 19.631592354513508 32.88206328170528 26.813248263093612 20.6730961006876 96-97 20.37983536181893 33.080420832103805 26.537278101678485 20.002465704398777 98-99 20.816225262035097 31.855139064820214 26.614632026309472 20.714003646835224 100-101 20.84265400530565 31.8349772263072 26.115309698376265 21.207059070010885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2114.0 1 1248.0 2 302.5 3 221.0 4 200.0 5 209.0 6 326.5 7 526.5 8 772.5 9 1070.0 10 1342.5 11 1616.0 12 1794.0 13 1680.0 14 1488.5 15 1377.5 16 1290.0 17 1214.5 18 1146.5 19 1144.0 20 1209.0 21 1374.5 22 1631.0 23 1837.5 24 2159.0 25 2699.0 26 3374.0 27 4249.5 28 5133.0 29 6135.5 30 8177.5 31 12041.5 32 21367.0 33 40013.0 34 70397.0 35 112510.5 36 170327.5 37 249058.5 38 323885.5 39 352567.0 40 356762.5 41 340101.5 42 322076.5 43 347175.5 44 356334.0 45 343787.5 46 353855.0 47 330934.5 48 263742.5 49 213221.0 50 177632.0 51 147222.0 52 116592.5 53 85943.5 54 64305.5 55 48010.0 56 36194.5 57 26101.0 58 17640.0 59 12454.5 60 8939.0 61 6554.5 62 4698.0 63 3204.5 64 2348.5 65 1750.0 66 1323.5 67 1025.5 68 812.5 69 595.5 70 420.0 71 317.5 72 251.0 73 214.5 74 186.0 75 148.5 76 110.5 77 97.0 78 88.0 79 69.5 80 49.0 81 48.5 82 44.0 83 33.5 84 30.5 85 27.5 86 25.0 87 19.5 88 19.5 89 15.5 90 9.5 91 7.5 92 4.5 93 3.0 94 3.5 95 2.0 96 2.0 97 4.5 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17250935227568184 2 0.16458894863118584 3 0.13196354763761933 4 0.12617128983218184 5 0.1389586704825247 6 0.159721971625339 7 0.13479490688436674 8 0.12617128983218184 9 0.1187875490514484 10-11 0.10612045490505231 12-13 0.1355813955640188 14-15 0.13129734546191404 16-17 0.12082316681054782 18-19 0.11977759950701039 20-21 0.11261592611817872 22-23 0.12524600903259117 24-25 0.42493520721200867 26-27 0.307461556895979 28-29 0.3946322610254147 30-31 0.22569449263615277 32-33 0.26564811756247725 34-35 0.27830595890087745 36-37 0.2755116108861137 38-39 0.2947296930936116 40-41 0.28704986245700914 42-43 0.3413175813530011 44-45 0.2903531149115478 46-47 0.31553925827640544 48-49 0.31559477512438083 50-51 0.31596488744421714 52-53 0.3189257860029072 54-55 0.29738524898843677 56-57 0.30909930391125445 58-59 0.30933062411115214 60-61 0.28806767133655886 62-63 0.28585625022553723 64-65 0.2937581482540414 66-67 0.2938599291419964 68-69 0.29101006427925713 70-71 0.2644637581390012 72-73 0.25488710186323793 74-75 0.264186173899124 76-77 0.26000390468497425 78-79 0.25731133755816543 80-81 0.26622179165822346 82-83 0.3071839726561018 84-85 0.31305950573350244 86-87 0.30215044510632866 88-89 0.3088217196713773 90-91 0.2861430872734103 92-93 0.2707001507282422 94-95 0.25659887134248066 96-97 0.2558308882788204 98-99 0.2772696444053359 100-101 0.2772418859813482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5403765.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.55748500335321 #Duplication Level Percentage of deduplicated Percentage of total 1 85.83176223371474 27.94466311735545 2 5.5979224969548 3.6450855548907883 3 2.4937417277159786 2.435698767070472 4 1.2347160844945109 1.607970016173161 5 0.708009019061401 1.1525496510165185 6 0.5798976684756608 1.1328005786925488 7 0.3514463056746961 0.8009545478541461 8 0.3037945423849148 0.7912629006237931 9 0.22404969137444036 0.656504502023633 >10 2.077263502430185 13.863958133558354 >50 0.2804006350682719 6.301991326719729 >100 0.25475507155718474 18.053027980194592 >500 0.04150595867578426 9.34955470146901 >1k 0.02027771916170868 10.734321904031331 >5k 4.0017723883471366E-4 0.9237113788897485 >10k+ 5.71660169963156E-5 0.6059449394365719 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 32557 0.6024873398454595 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 8926 0.16518112834292387 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 8892 0.16455193739920224 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8140 0.15063571417335875 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8050 0.14897020873409558 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 5435 0.10057802291550427 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005755246573453879 0.0 0.0 2 0.006254898205232833 0.0 0.0 3 0.006884089148954479 0.0 0.0 4 0.007217190236807115 0.0 0.0 5 0.007994426108463265 0.0 0.0 6 0.008938212524045735 0.0 0.0 7 0.010030043867562708 0.0 0.0 8 0.010973830283145178 0.0 0.0 9 0.0124172683305066 0.0 0.0 10-11 0.019181070975514296 0.0 0.0 12-13 0.02529717706080853 0.0 0.0 14-15 0.03094138993831153 0.0 0.0 16-17 0.03547526585630574 0.0 9.252807995906558E-6 18-19 0.04113798434980055 0.0 1.8505615991813116E-5 20-21 0.04697650619521759 0.0 1.8505615991813116E-5 22-23 0.05335169090439722 0.0 1.8505615991813116E-5 24-25 0.06008773512541719 0.0 1.8505615991813116E-5 26-27 0.06750848713813425 0.0 4.626403997953279E-5 28-29 0.07616911542230279 0.0 5.5516847975439346E-5 30-31 0.11660388636441443 0.0 5.5516847975439346E-5 32-33 0.12816064355130175 0.0 5.5516847975439346E-5 34-35 0.13779281667504045 0.0 5.5516847975439346E-5 36-37 0.1488776806541365 0.0 5.5516847975439346E-5 38-39 0.16130420179263902 0.0 5.5516847975439346E-5 40-41 0.17471152057870765 9.252807995906558E-6 5.5516847975439346E-5 42-43 0.18934946282823178 2.7758423987719676E-5 5.5516847975439346E-5 44-45 0.20450556232552675 3.701123198362623E-5 5.5516847975439346E-5 46-47 0.22035562242251466 3.701123198362623E-5 5.5516847975439346E-5 48-49 0.23745481159895 3.701123198362623E-5 6.47696559713459E-5 50-51 0.2552479613750783 3.701123198362623E-5 7.402246396725247E-5 52-53 0.27478989186243297 3.701123198362623E-5 7.402246396725247E-5 54-55 0.29660801311678064 3.701123198362623E-5 7.402246396725247E-5 56-57 0.31940693201869436 3.701123198362623E-5 7.402246396725247E-5 58-59 0.34546283933516725 3.701123198362623E-5 7.402246396725247E-5 60-61 0.3728048869630711 3.701123198362623E-5 7.402246396725247E-5 62-63 0.4016829007182955 3.701123198362623E-5 7.402246396725247E-5 64-65 0.43156947054507366 3.701123198362623E-5 7.402246396725247E-5 66-67 0.462631146987332 3.701123198362623E-5 7.402246396725247E-5 68-69 0.495700682764702 3.701123198362623E-5 7.402246396725247E-5 70-71 0.5322770327725206 3.701123198362623E-5 7.402246396725247E-5 72-73 0.5737481182101738 4.626403997953279E-5 7.402246396725247E-5 74-75 0.6181338381665376 5.5516847975439346E-5 7.402246396725247E-5 76-77 0.6649530466258248 5.5516847975439346E-5 7.402246396725247E-5 78-79 0.7154918838994664 5.5516847975439346E-5 7.402246396725247E-5 80-81 0.772165332874394 5.5516847975439346E-5 7.402246396725247E-5 82-83 0.8328637533275411 5.5516847975439346E-5 7.402246396725247E-5 84-85 0.8985401844824858 5.5516847975439346E-5 7.402246396725247E-5 86-87 0.9701569183708025 5.5516847975439346E-5 7.402246396725247E-5 88-89 1.0459189102412854 5.5516847975439346E-5 7.402246396725247E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 6010 0.0 90.349686 9 CGGGAGA 8095 0.0 89.93889 4 AGAGCGG 6345 0.0 88.790886 8 GAGAGCG 7230 0.0 88.02333 7 ATTCGGA 1440 0.0 87.95186 2 CGGAGAG 2550 0.0 87.04858 5 CGGAAAG 3080 0.0 85.774956 5 AAGCGGC 1985 0.0 84.09647 9 GTCCGGA 430 0.0 83.8381 2 CCGGAGA 1150 0.0 83.724815 4 TCGGGAG 8780 0.0 83.40821 3 GTTCGGA 1035 0.0 82.95345 2 TCGGAGA 1825 0.0 80.04678 4 AAGCGTC 880 0.0 79.75808 9 TTCGGAG 1310 0.0 79.65396 3 AAGGGGC 4955 0.0 78.86407 9 GATCCGG 1600 0.0 77.381454 1 TCGGGAA 2525 0.0 77.20341 3 GGAAAGC 2410 0.0 77.14806 6 GATCCGA 335 0.0 76.46547 1 >>END_MODULE