##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2859530 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.73100229758037 33.0 33.0 33.0 22.0 33.0 2 30.110554531688773 33.0 33.0 33.0 22.0 33.0 3 29.897657307319733 33.0 33.0 33.0 15.0 33.0 4 33.120472944854576 37.0 37.0 37.0 15.0 37.0 5 33.25050550265254 37.0 37.0 37.0 15.0 37.0 6 34.07648529653475 37.0 37.0 37.0 27.0 37.0 7 33.14515672155914 37.0 37.0 37.0 15.0 37.0 8 33.722685196518306 37.0 37.0 37.0 22.0 37.0 9 32.884965011732696 37.0 37.0 37.0 15.0 37.0 10-11 32.98323448258979 37.0 37.0 37.0 15.0 37.0 12-13 32.93403024273219 37.0 37.0 37.0 15.0 37.0 14-15 34.37411759974542 40.0 37.0 40.0 15.0 40.0 16-17 34.3018146338734 40.0 37.0 40.0 15.0 40.0 18-19 34.38935506883998 40.0 37.0 40.0 15.0 40.0 20-21 34.36881130815204 40.0 37.0 40.0 15.0 40.0 22-23 35.41485244078572 38.5 37.0 40.0 24.5 40.0 24-25 34.49583148279612 37.0 37.0 40.0 15.0 40.0 26-27 34.43911446986043 37.0 37.0 40.0 18.5 40.0 28-29 33.94047920462454 37.0 37.0 40.0 15.0 40.0 30-31 33.93669344262868 37.0 37.0 40.0 10.5 40.0 32-33 33.80377649473864 37.0 33.0 40.0 10.5 40.0 34-35 33.91554276402066 37.0 33.0 40.0 15.0 40.0 36-37 33.78012610463957 37.0 33.0 40.0 10.5 40.0 38-39 33.59307456120412 37.0 33.0 40.0 15.0 40.0 40-41 33.62390183002102 37.0 33.0 40.0 15.0 40.0 42-43 33.44262903344256 37.0 33.0 40.0 15.0 40.0 44-45 33.23711588967418 37.0 33.0 40.0 6.0 40.0 46-47 33.136356848852785 37.0 33.0 40.0 6.0 40.0 48-49 33.03358296643155 37.0 33.0 40.0 10.5 40.0 50-51 32.18983626679909 37.0 33.0 38.5 15.0 38.5 52-53 32.6295247820446 37.0 33.0 38.5 10.5 40.0 54-55 33.131635968148615 37.0 33.0 40.0 10.5 40.0 56-57 33.53421418904505 37.0 33.0 40.0 18.5 40.0 58-59 32.851280804887516 37.0 33.0 40.0 6.0 40.0 60-61 32.76188167286232 37.0 33.0 40.0 10.5 40.0 62-63 32.32969264179778 37.0 33.0 40.0 6.0 40.0 64-65 32.166465642955316 37.0 33.0 40.0 6.0 40.0 66-67 32.118169419450055 37.0 33.0 37.0 10.5 40.0 68-69 32.342634279059844 37.0 33.0 37.0 15.0 40.0 70-71 31.861222298769377 37.0 33.0 37.0 15.0 40.0 72-73 32.37473343521488 37.0 33.0 37.0 18.5 40.0 74-75 32.551125884323646 37.0 33.0 37.0 22.0 37.0 76-77 32.18270624892902 37.0 33.0 37.0 22.0 37.0 78-79 31.59307665945103 37.0 30.0 37.0 18.5 37.0 80-81 30.95061111441391 37.0 27.0 37.0 15.0 37.0 82-83 30.534464929551362 33.0 27.0 37.0 15.0 37.0 84-85 30.25293107608593 33.0 27.0 37.0 6.0 37.0 86-87 30.071974065668137 33.0 27.0 37.0 6.0 37.0 88-89 29.910537570859546 33.0 27.0 37.0 6.0 37.0 90-91 29.759860886229554 33.0 27.0 37.0 6.0 37.0 92-93 29.5927797575126 33.0 27.0 37.0 6.0 37.0 94-95 29.44656656863191 33.0 27.0 37.0 6.0 37.0 96-97 29.32750924102912 33.0 27.0 37.0 6.0 37.0 98-99 29.17222130909625 33.0 27.0 37.0 6.0 37.0 100-101 28.00641731333471 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.01576289124839292 2306 2 -0.18086010820250564 2306 3 -0.12553890601760642 2306 4 0.10567521199314456 2306 5 0.0678555062056958 2306 6 -0.32533267582391545 2306 7 0.4542797436716768 2306 8 -0.27453098260109954 2306 9 0.040363000264619586 2306 10-11 0.0886449617058318 2306 12-13 -0.07547963804677948 2306 14-15 0.33794373352422724 2306 16-17 0.3156219074519626 2306 18-19 0.17085642217021046 2306 20-21 0.08811700100880415 2306 22-23 0.02712305720034891 2306 24-25 0.26584425143091295 2306 26-27 0.09304130916112996 2306 28-29 0.41212180034553825 2306 30-31 0.43332310126670137 2306 32-33 0.41581121714221325 2306 34-35 0.3704500937075679 2306 36-37 0.23763295537744966 2306 38-39 0.2590350477313095 2306 40-41 0.2916256751866655 2306 42-43 0.32468701970306313 2306 44-45 0.4422717434541781 2306 46-47 0.2623809057139681 2306 48-49 0.4303210398858113 2306 50-51 0.3679739374461377 2306 52-53 0.293375167782564 2306 54-55 0.5567055974826332 2306 56-57 0.36640409126242446 2306 58-59 0.7225610847409101 2306 60-61 0.5627906232014013 2306 62-63 0.5581387643918418 2306 64-65 0.6305933723495585 2306 66-67 0.3106370886815242 2306 68-69 0.3143810865736185 2306 70-71 0.42600706636457275 2306 72-73 0.0830397602234001 2306 74-75 0.146454332563124 2306 76-77 0.3619978448441188 2306 78-79 0.5483417502598691 2306 80-81 0.4490434267969725 2306 82-83 0.5293233717973109 2306 84-85 0.587386120054024 2306 86-87 0.4327691898874235 2306 88-89 0.4418454192335126 2306 90-91 0.573366127216552 2306 92-93 0.3776008814272096 2306 94-95 0.31201090075979465 2306 96-97 0.3646826402239718 2306 98-99 0.4648294543713689 2306 100-101 0.42804004534922413 2307 1 -0.12560487208701332 2307 2 -0.2736974023835863 2307 3 -0.1973857808879984 2307 4 -0.022689434848544465 2307 5 -0.17509169631597388 2307 6 -0.2809214112558749 2307 7 0.22329803407854598 2307 8 -0.3610931326570892 2307 9 -0.05596440216580589 2307 10-11 -0.07416869181330554 2307 12-13 -0.18333704752793523 2307 14-15 0.16188923557723456 2307 16-17 0.06744991126765143 2307 18-19 0.08986330699455891 2307 20-21 0.09170975950078031 2307 22-23 0.03229600730611537 2307 24-25 0.23249743877278917 2307 26-27 0.01787748352138152 2307 28-29 0.13464575206881335 2307 30-31 0.23849397763018487 2307 32-33 0.2622526753977823 2307 34-35 0.08477965282996536 2307 36-37 0.06411775484657056 2307 38-39 0.06193310256105633 2307 40-41 0.020402996741147206 2307 42-43 0.047202676457246184 2307 44-45 0.09154697052895955 2307 46-47 6.974417348786233E-4 2307 48-49 0.0695956448379107 2307 50-51 0.13271617128131652 2307 52-53 0.0019366127661868404 2307 54-55 0.2885168039858925 2307 56-57 0.2643241277602897 2307 58-59 0.31054685886889644 2307 60-61 0.1461716526070731 2307 62-63 0.03690882784835736 2307 64-65 0.32029516821037163 2307 66-67 0.2734362260047405 2307 68-69 0.024667885130298828 2307 70-71 0.16005791452516505 2307 72-73 -0.15696480200960394 2307 74-75 0.08413837719005812 2307 76-77 0.1844668423971072 2307 78-79 0.20288531958507505 2307 80-81 0.23153689450880677 2307 82-83 0.33463215202210606 2307 84-85 0.29507348266860234 2307 86-87 0.34755600918152396 2307 88-89 0.3728136495018539 2307 90-91 0.4015617640628051 2307 92-93 0.3756401581059059 2307 94-95 0.3319862232267212 2307 96-97 0.37073485705446885 2307 98-99 0.2090882988821754 2307 100-101 0.4806311863222241 2201 1 0.07980372800243885 2201 2 0.020520219493612046 2201 3 0.05649576022758751 2201 4 0.17745752061755837 2201 5 0.026464114814302775 2201 6 0.0507091554810728 2201 7 0.42812200698762837 2201 8 0.005554365905304337 2201 9 0.3008945844804174 2201 10-11 0.19452979390644742 2201 12-13 0.17557931564375195 2201 14-15 0.42310257657780426 2201 16-17 0.3316023615903063 2201 18-19 0.36021024968192705 2201 20-21 0.21000731237280235 2201 22-23 0.2316315130246025 2201 24-25 0.46656075741058345 2201 26-27 0.19682930768597373 2201 28-29 0.3441195876064782 2201 30-31 0.43639986223819704 2201 32-33 0.2512150420197159 2201 34-35 0.2859089736401401 2201 36-37 0.4537174609356427 2201 38-39 0.4265328086501299 2201 40-41 0.3102311227394807 2201 42-43 0.2330150846100736 2201 44-45 0.2508658638904038 2201 46-47 0.24868958281211917 2201 48-49 0.2840787449593094 2201 50-51 0.4019737267090804 2201 52-53 0.39275349347668254 2201 54-55 0.5195803143047186 2201 56-57 0.551660400202941 2201 58-59 0.7542756940870987 2201 60-61 0.3521009861959783 2201 62-63 0.4479720186987777 2201 64-65 0.5660670038758226 2201 66-67 0.47979045257173425 2201 68-69 0.37077037059447804 2201 70-71 0.42707919112090664 2201 72-73 0.2543795210368458 2201 74-75 0.32507505855227237 2201 76-77 0.4249370998886448 2201 78-79 0.5830079954524301 2201 80-81 0.5001379423651748 2201 82-83 0.45760033296900815 2201 84-85 0.3568411013511117 2201 86-87 0.4162065746405972 2201 88-89 0.5161671093446749 2201 90-91 0.4830613487538784 2201 92-93 0.5301270918481613 2201 94-95 0.41403529357366153 2201 96-97 0.37945794184138393 2201 98-99 0.2596282004859063 2201 100-101 0.42606438547882775 1101 1 0.08312068131938943 1101 2 -0.10244690347351337 1101 3 0.08901009274191907 1101 4 0.12587216903220622 1101 5 0.06819220654239189 1101 6 0.16316381793573953 1101 7 0.29152437038999324 1101 8 0.1657743261252662 1101 9 0.18818837177420278 1101 10-11 0.18653868591534462 1101 12-13 0.06751800758244286 1101 14-15 0.23511848859371298 1101 16-17 0.25195453194248074 1101 18-19 0.26377875325043476 1101 20-21 0.3402606176261074 1101 22-23 0.30287873427182177 1101 24-25 0.2254205912704137 1101 26-27 0.31679830265496634 1101 28-29 0.2972873657742596 1101 30-31 0.3572054080437468 1101 32-33 0.4925219333266142 1101 34-35 0.2820333447645069 1101 36-37 0.4043989866171671 1101 38-39 0.36520134731865994 1101 40-41 0.25815437066273006 1101 42-43 0.13251783411282503 1101 44-45 0.13294729597183874 1101 46-47 0.33183864096118043 1101 48-49 0.16866690454746447 1101 50-51 0.046585871321227046 1101 52-53 0.16990044562363238 1101 54-55 0.3539461736705789 1101 56-57 0.48467198321453253 1101 58-59 0.4024153922267928 1101 60-61 0.2975087316037204 1101 62-63 0.4701669408936979 1101 64-65 0.6274364902453087 1101 66-67 0.431808704589983 1101 68-69 0.2576312574553654 1101 70-71 0.3099796240213486 1101 72-73 0.34132815798547966 1101 74-75 0.4764966099738217 1101 76-77 0.3775696025211417 1101 78-79 0.5209335833780209 1101 80-81 0.42849584572307364 1101 82-83 0.3600719604406386 1101 84-85 0.4057793252893376 1101 86-87 0.4650424234764472 1101 88-89 0.4470054151829821 1101 90-91 0.45716337285590214 1101 92-93 0.36942450614557387 1101 94-95 0.35396743350580095 1101 96-97 0.31447315185659264 1101 98-99 0.3754817163394186 1101 100-101 0.48342661784106156 2304 1 0.1509873900006582 2304 2 -0.0846923587692281 2304 3 0.0200982708838211 2304 4 -0.04505485310798463 2304 5 -0.07191598105279695 2304 6 0.01256060832039907 2304 7 0.008578546716265123 2304 8 -0.03191186982783023 2304 9 -0.01798139722052383 2304 10-11 0.10610098381386024 2304 12-13 -0.005263659046711666 2304 14-15 0.1579215376532659 2304 16-17 0.1370144027475888 2304 18-19 0.06897881027025221 2304 20-21 0.12686602550534332 2304 22-23 0.14732155315137874 2304 24-25 0.2512036758923202 2304 26-27 0.19224283212896154 2304 28-29 0.16472398750031658 2304 30-31 0.19243371237950413 2304 32-33 0.11241697185250388 2304 34-35 0.12864516737286635 2304 36-37 0.16282366419262218 2304 38-39 0.03394923721040044 2304 40-41 0.11099579555787642 2304 42-43 0.05463514487831844 2304 44-45 0.04341118217229223 2304 46-47 0.028564128067948502 2304 48-49 0.08354476210296724 2304 50-51 0.05787954587313493 2304 52-53 0.03802805746875748 2304 54-55 0.3201911938150843 2304 56-57 0.4918808633003593 2304 58-59 0.4410455662122601 2304 60-61 0.2332558412243131 2304 62-63 0.28329256454792073 2304 64-65 0.3376691085801795 2304 66-67 0.3617049060199804 2304 68-69 0.30063788976009675 2304 70-71 0.3645575273427504 2304 72-73 0.4888534230150796 2304 74-75 0.5905043526644036 2304 76-77 0.5986427379356982 2304 78-79 0.5037595529024337 2304 80-81 0.48630005768673357 2304 82-83 0.36676860002289047 2304 84-85 0.3455478323455381 2304 86-87 0.2257643993630687 2304 88-89 0.28706933739595186 2304 90-91 0.31530407675050753 2304 92-93 0.4080222313550834 2304 94-95 0.41127872417931144 2304 96-97 0.33092092995948263 2304 98-99 0.377111845309237 2304 100-101 0.36432066602280244 2202 1 0.060738163020886304 2202 2 -0.005232721311838162 2202 3 0.06424434895633624 2202 4 0.07052152978425141 2202 5 -0.12716156454066407 2202 6 0.17863760902213244 2202 7 0.031152692282020666 2202 8 0.10116759874140513 2202 9 -0.09298727871299661 2202 10-11 -0.026470552998212327 2202 12-13 0.08828864107186263 2202 14-15 0.0774010407362482 2202 16-17 0.09366097796631578 2202 18-19 0.13350805156782997 2202 20-21 0.18766270989401335 2202 22-23 0.1776899401798815 2202 24-25 0.2222271297338949 2202 26-27 0.15085524143768936 2202 28-29 0.2617152754763836 2202 30-31 0.3003194118928647 2202 32-33 0.44189329319971904 2202 34-35 0.1983494479534258 2202 36-37 0.3392638883432113 2202 38-39 0.2638392516158419 2202 40-41 0.3182593376521794 2202 42-43 0.09121044449738491 2202 44-45 0.17981243005984737 2202 46-47 0.32307303861330894 2202 48-49 0.3093820237339955 2202 50-51 0.2813828324309071 2202 52-53 0.36600004808263265 2202 54-55 0.46588604243658693 2202 56-57 0.5661494516814898 2202 58-59 0.5403280241300905 2202 60-61 0.3789303826869812 2202 62-63 0.47306111012392904 2202 64-65 0.43974561247275545 2202 66-67 0.48620135589045077 2202 68-69 0.574301965199588 2202 70-71 0.4889811747848576 2202 72-73 0.3925826599984461 2202 74-75 0.4191461424716678 2202 76-77 0.48807059511081974 2202 78-79 0.46014592684940325 2202 80-81 0.5844539094407679 2202 82-83 0.4428266619818011 2202 84-85 0.3972667954232456 2202 86-87 0.4315360815103624 2202 88-89 0.4089995859286084 2202 90-91 0.2936740034155392 2202 92-93 0.27611324719837427 2202 94-95 0.39828214252296235 2202 96-97 0.33111178354772974 2202 98-99 0.3263821189317717 2202 100-101 0.41224733038406214 1102 1 0.021515372956024237 1102 2 -0.06340342256267206 1102 3 -0.015313618621437541 1102 4 -0.12100971282558959 1102 5 -0.12580332491145185 1102 6 0.05819324201295473 1102 7 0.025899295205817907 1102 8 0.14892344821473102 1102 9 -0.11864885670066627 1102 10-11 0.012943687607489096 1102 12-13 -0.034324586034877314 1102 14-15 -0.07243080415379666 1102 16-17 0.03282286701607262 1102 18-19 0.06698853593410092 1102 20-21 0.028335004644553408 1102 22-23 0.08068605107506244 1102 24-25 -0.016773326951664558 1102 26-27 0.08956527262460412 1102 28-29 -0.036634128317665215 1102 30-31 0.03505009639231815 1102 32-33 -0.09495571191316543 1102 34-35 0.09108231111247278 1102 36-37 0.13841591448366586 1102 38-39 0.06372292577724892 1102 40-41 -0.08359541962355621 1102 42-43 0.0875035671645108 1102 44-45 -0.01634922077558798 1102 46-47 -0.01727270218498944 1102 48-49 -0.059254371410126794 1102 50-51 -0.0801456101157001 1102 52-53 0.02866527595201518 1102 54-55 0.35035706178209836 1102 56-57 0.3635680470124072 1102 58-59 0.16748879603677125 1102 60-61 0.16385108382973357 1102 62-63 0.2097107674512344 1102 64-65 0.2904843723372821 1102 66-67 0.37790570379924304 1102 68-69 0.2256063291910948 1102 70-71 0.1337668532179883 1102 72-73 0.2763127362082969 1102 74-75 0.270212897391481 1102 76-77 0.24694458347448744 1102 78-79 0.22416638244260767 1102 80-81 0.19270200513116365 1102 82-83 0.09018989733886329 1102 84-85 0.1529184773562413 1102 86-87 0.2172064444622528 1102 88-89 0.30149409064238597 1102 90-91 0.27931571723870263 1102 92-93 0.24410015160669474 1102 94-95 0.1870047617450581 1102 96-97 0.3184537828101739 1102 98-99 0.23616171807166708 1102 100-101 0.27515555705054595 2305 1 0.1058959356217386 2305 2 0.034776136212581577 2305 3 -0.02432665261452982 2305 4 -0.02969262741928702 2305 5 0.006031542903897957 2305 6 0.222814512069192 2305 7 -0.01658131702604493 2305 8 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74-75 -0.35892413366610043 1213 76-77 -0.6634936721037761 1213 78-79 -0.7237470784259301 1213 80-81 -0.8493580355554649 1213 82-83 -0.7225823715287625 1213 84-85 -0.626617045463199 1213 86-87 -0.7973456450760068 1213 88-89 -0.6381929480975757 1213 90-91 -0.66821763882648 1213 92-93 -0.583807421743888 1213 94-95 -0.5764749551557671 1213 96-97 -0.5198742359154558 1213 98-99 -0.74983406377088 1213 100-101 -1.0750297659577868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1286.0 3 197.0 4 324.0 5 297.0 6 575.0 7 3821.0 8 18232.0 9 60862.0 10 88477.0 11 67208.0 12 39959.0 13 25874.0 14 17884.0 15 14100.0 16 12184.0 17 11360.0 18 10918.0 19 10949.0 20 11449.0 21 11979.0 22 12522.0 23 13007.0 24 14762.0 25 16327.0 26 19261.0 27 23168.0 28 28791.0 29 35534.0 30 42103.0 31 53810.0 32 72629.0 33 104543.0 34 157181.0 35 262031.0 36 501460.0 37 830054.0 38 264000.0 39 412.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.42142327974682 21.928098679981854 23.41568924842857 13.234788791842753 2 30.13815029286405 27.598535802336816 30.81557463033422 11.44773927446491 3 35.78202910242149 19.516458214506674 30.392244396196006 14.30926828687583 4 26.938133295800803 17.449510519086797 35.13450965604867 20.47784652906373 5 35.774238052551965 16.065816012101518 31.607933217547203 16.552012717799318 6 31.811100783186667 16.210934353392933 37.324648070332 14.6533167930884 7 35.79638085100423 19.685390347068545 28.464732905683633 16.053495896243593 8 26.776449317604683 33.66510409500212 24.186984824883787 15.371461762509414 9 34.10381554639015 27.178823082117905 22.476493955838713 16.240867415653238 10-11 27.08367754419668 30.922760482445945 26.498664928576833 15.49489704478055 12-13 29.578886829829383 23.62887207988256 26.471471326891212 20.320769763396846 14-15 25.16416481168468 24.13219668090254 29.2550631598389 21.448575347573875 16-17 27.627075831422243 25.030252711891077 30.36185446804503 16.980816988641646 18-19 27.460743821544447 25.205700537127623 30.80079015613198 16.53276548519595 20-21 25.407516421781075 29.127657205909742 28.948246359622654 16.516580012686536 22-23 29.8645191490643 25.725431404066317 27.821925448419698 16.588123998449685 24-25 23.98253453582936 31.557655571650557 27.689144091483893 16.770665801036195 26-27 22.590501369881753 33.16937658849903 27.59983354134819 16.64028850027103 28-29 24.57584458985088 30.955832795079896 27.489153023112134 16.97916959195709 30-31 28.046839269286373 26.826074385596005 28.076067814430278 17.051018530687344 32-33 27.480744075093288 26.567671923706254 29.09952496968171 16.85205903151875 34-35 22.454377765618123 26.594816772074058 28.10512324976774 22.84568221254008 36-37 18.971470527750267 26.85307275331874 29.426947324203777 24.748509394727215 38-39 19.117569181292602 29.252959526686155 31.611532641155087 20.017938650866157 40-41 21.761050157261984 26.620583943602405 33.9635385050766 17.65482739405901 42-43 23.451242353492674 26.214721855554785 32.963437817763825 17.370597973188715 44-45 20.914000554088506 29.217823414096994 32.54268760980948 17.325488422005026 46-47 24.032706331603155 30.71516292304902 27.938958798048102 17.313171947299725 48-49 19.881165481197893 29.25987390779547 31.242372677426562 19.616587933580075 50-51 19.07440695793462 26.922586505452788 32.83091677969095 21.17208975692164 52-53 26.444185364096267 26.32568738387645 27.728790466420044 19.501336785607233 54-55 27.983758328416 26.594851660159417 27.743903454818536 17.67748655660605 56-57 28.736103891366398 26.318231848860286 27.309874015162517 17.635790244610796 58-59 26.024919141867308 26.35885682857936 27.65242911124276 19.963794918310573 60-61 20.774284363659827 26.518978197411407 27.23375536718488 25.472982071743882 62-63 24.752358935110546 26.5865553629775 27.726296022576342 20.934789679335612 64-65 20.594553581558188 28.931518129390003 31.046647239011538 19.427281050040268 66-67 19.012003636774914 28.935261373469956 28.90438210334999 23.148352886405142 68-69 18.68794060112881 27.022740997551466 35.68845471614609 18.60086368517364 70-71 18.186586282105356 32.78221456521411 31.08552464354068 17.945674509139852 72-73 18.11767489757017 36.839560921101636 26.866054643850923 18.17670953747727 74-75 18.5371110872317 36.98606507848331 26.392926817339514 18.08389701694548 76-77 18.76095782763576 36.543235499207164 26.56194947926545 18.13385719389163 78-79 19.01884645378553 36.04552779062713 26.804299176914842 18.131326578672493 80-81 18.555412470080313 36.07535045520699 26.721054729668374 18.648182345044333 82-83 18.427094809525464 35.27482005506117 26.93272667134571 19.365358464067658 84-85 18.692405896050737 34.09153530550032 27.547619135313006 19.668439663135935 86-87 18.852878606160225 33.72060457805468 27.858188927923884 19.568327887861212 88-89 19.319272972169536 33.935612069426654 27.352497305696666 19.392617652707152 90-91 19.834320074427268 33.369148943258324 26.92531989453098 19.87121108778343 92-93 19.6153704557527 33.27792377182387 27.231210741577684 19.875495030845748 94-95 19.177693925832806 33.257043916867026 27.359668110363682 20.205594046936483 96-97 19.51808246819854 33.87585528977312 26.87956917023483 19.72649307179351 98-99 20.00997924380404 32.96930365671261 26.84502490340278 20.17569219608057 100-101 20.23730941795235 32.878161260512115 26.315246896924616 20.569282424610922 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1115.0 1 679.0 2 209.0 3 240.0 4 297.0 5 286.0 6 340.5 7 459.5 8 633.5 9 836.5 10 971.5 11 1090.0 12 1138.0 13 1107.5 14 1052.5 15 1005.0 16 1019.5 17 989.0 18 996.0 19 1091.0 20 1259.5 21 1543.5 22 1962.0 23 2676.0 24 3759.0 25 5385.0 26 8218.5 27 12190.5 28 16571.5 29 20850.0 30 25383.0 31 30107.5 32 36705.0 33 47708.0 34 63877.0 35 87167.5 36 112199.5 37 139732.0 38 171300.0 39 186946.5 40 183112.0 41 169031.5 42 155271.5 43 152757.5 44 147657.5 45 140979.5 46 143329.0 47 135556.0 48 113047.5 49 93934.5 50 80885.5 51 69482.5 52 60021.0 53 50934.0 54 42096.5 55 33735.5 56 25440.5 57 18988.0 58 14066.0 59 10352.0 60 7758.5 61 5580.5 62 3941.0 63 2835.0 64 2023.5 65 1475.0 66 1112.5 67 805.0 68 568.0 69 435.5 70 325.0 71 242.0 72 205.0 73 160.0 74 117.0 75 102.5 76 88.0 77 71.5 78 61.5 79 56.0 80 47.0 81 43.0 82 35.5 83 23.0 84 24.0 85 24.5 86 21.0 87 21.0 88 19.0 89 19.0 90 20.0 91 15.5 92 10.0 93 6.5 94 5.5 95 5.5 96 3.5 97 1.5 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1624392819799058 2 0.15565495028903353 3 0.1216633502708487 4 0.11683738236703235 5 0.1290421852542201 6 0.14939518032683693 7 0.12624452270128306 8 0.11683738236703235 9 0.10970334285704295 10-11 0.09630953338485695 12-13 0.12545768010826955 14-15 0.12178574800753972 16-17 0.11101474717873218 18-19 0.10954597433844022 20-21 0.10344357289484636 22-23 0.11655761611173864 24-25 0.40249621441285804 26-27 0.2927579007739034 28-29 0.37803415246561495 30-31 0.21524516266659208 32-33 0.25497197091829776 34-35 0.26782373327085224 36-37 0.26523589540938547 38-39 0.2848370186709005 40-41 0.2769336219588534 42-43 0.3334813763100929 44-45 0.2797662552937021 46-47 0.3065888450199858 48-49 0.3063090787646921 50-51 0.30569709008123713 52-53 0.3094389637457904 54-55 0.2875472542690582 56-57 0.29907012690896756 58-59 0.2989652145632324 60-61 0.2777379499428228 62-63 0.27530748059995874 64-65 0.2831409357481824 66-67 0.2833507604396527 68-69 0.28074543718723005 70-71 0.2532234318227121 72-73 0.2436589229698587 74-75 0.25303109252219774 76-77 0.24888705486565973 78-79 0.2458620822302966 80-81 0.25567138655653204 82-83 0.297916091105881 84-85 0.3044731127143272 86-87 0.29321252093875566 88-89 0.30101100530506764 90-91 0.2758145569376786 92-93 0.2600077635135844 94-95 0.24533752050162091 96-97 0.24437582399904878 98-99 0.26614513573909 100-101 0.2672292299783531 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2859530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.90590019903686 #Duplication Level Percentage of deduplicated Percentage of total 1 92.28840959587151 56.20908664373986 2 3.6453039917886203 4.440410422380568 3 1.169547364606345 2.1369700520028188 4 0.5698395389090014 1.3882636034502727 5 0.4489593007695429 1.3672135183049572 6 0.25403702138590356 0.928341208283426 7 0.19627080225946564 0.8367834926079938 8 0.16483254777606363 0.8031419763521523 9 0.13823467695957276 0.7577376695051254 >10 0.9353853267505227 11.035335208401516 >50 0.10068769063340435 4.23970070712749 >100 0.07799391392867756 9.935167901449317 >500 0.008257898805599016 3.345765307694063 >1k 0.0021824929464082255 2.072563167223657 >5k 0.0 0.0 >10k+ 5.783660919866799E-5 0.5035191214769198 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 14294 0.49987235664602225 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3384 0.11834112598923599 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3246 0.11351515808541963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004825967903816362 0.0 0.0 2 0.005420471196315478 0.0 0.0 3 0.005910062143079457 0.0 0.0 4 0.006399653089843436 0.0 0.0 5 0.007693572020576808 0.0 0.0 6 0.008672753914104766 0.0 0.0 7 0.010141526754396701 0.0 0.0 8 0.01164527037660035 0.0 0.0 9 0.012869247743510297 0.0 0.0 10-11 0.020352995072616828 0.0 0.0 12-13 0.0252139337583449 0.0 0.0 14-15 0.030512007217969386 0.0 0.0 16-17 0.035355460512741604 0.0 0.0 18-19 0.04046119467185167 0.0 0.0 20-21 0.045462016485226596 0.0 0.0 22-23 0.05131962245543848 0.0 0.0 24-25 0.05812143953726662 0.0 1.7485390955856382E-5 26-27 0.06523799365630016 0.0 3.4970781911712765E-5 28-29 0.07373589366084637 0.0 3.4970781911712765E-5 30-31 0.1399355838197186 0.0 3.4970781911712765E-5 32-33 0.1509338947309523 0.0 3.4970781911712765E-5 34-35 0.15997384185513003 0.0 6.994156382342553E-5 36-37 0.16894384741548435 0.0 6.994156382342553E-5 38-39 0.17969736285333604 0.0 6.994156382342553E-5 40-41 0.1905732760278787 0.0 6.994156382342553E-5 42-43 0.20211363405874394 0.0 6.994156382342553E-5 44-45 0.21620685916916416 0.0 6.994156382342553E-5 46-47 0.23143663469171505 0.0 6.994156382342553E-5 48-49 0.24650904169566326 0.0 6.994156382342553E-5 50-51 0.2621060104282872 0.0 6.994156382342553E-5 52-53 0.28015093389473095 0.0 6.994156382342553E-5 54-55 0.29919252464565854 0.0 6.994156382342553E-5 56-57 0.3199826544921718 0.0 6.994156382342553E-5 58-59 0.34119243372162555 0.0 6.994156382342553E-5 60-61 0.3651998755040164 0.0 6.994156382342553E-5 62-63 0.3913230495920658 0.0 6.994156382342553E-5 64-65 0.4182155808821729 0.0 6.994156382342553E-5 66-67 0.446576885012572 0.0 6.994156382342553E-5 68-69 0.4794669054005378 0.0 6.994156382342553E-5 70-71 0.5136508447192372 0.0 6.994156382342553E-5 72-73 0.5509471836280787 0.0 6.994156382342553E-5 74-75 0.5921427647200763 0.0 6.994156382342553E-5 76-77 0.6366605700936867 0.0 6.994156382342553E-5 78-79 0.6846579682675125 0.0 6.994156382342553E-5 80-81 0.7391424464859611 0.0 6.994156382342553E-5 82-83 0.7968267512493312 0.0 6.994156382342553E-5 84-85 0.8623095403790133 0.0 6.994156382342553E-5 86-87 0.9310795830083964 0.0 6.994156382342553E-5 88-89 1.0098162984826178 0.0 6.994156382342553E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 3780 0.0 90.21173 9 CGGGAGA 4865 0.0 87.944855 4 GAGAGCG 4645 0.0 85.25335 7 ATTCGGA 920 0.0 84.04598 2 GATCGGG 6450 0.0 82.59909 1 ATCGGGA 6340 0.0 82.45358 2 TCGGGAG 5035 0.0 81.772446 3 AAGCGGC 1420 0.0 80.82634 9 AGAGCGG 4525 0.0 78.50077 8 CGGAAAG 1880 0.0 78.215096 5 AAGCGTC 655 0.0 77.47622 9 TCGGAGA 1150 0.0 76.30634 4 GTTCGGA 630 0.0 74.54385 2 TCGGGAA 1970 0.0 74.16026 3 ATCCGGA 935 0.0 74.07274 2 GAACCGG 495 0.0 72.838936 1 TTCGGAG 905 0.0 72.329796 3 GATCCGG 995 0.0 71.51933 1 AAAGCGG 1775 0.0 71.07126 8 CGGAGAG 1985 0.0 70.97129 5 >>END_MODULE