FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859530
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT142940.49987235664602225Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC33840.11834112598923599No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG32460.11351515808541963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC37800.090.211739
CGGGAGA48650.087.9448554
GAGAGCG46450.085.253357
ATTCGGA9200.084.045982
GATCGGG64500.082.599091
ATCGGGA63400.082.453582
TCGGGAG50350.081.7724463
AAGCGGC14200.080.826349
AGAGCGG45250.078.500778
CGGAAAG18800.078.2150965
AAGCGTC6550.077.476229
TCGGAGA11500.076.306344
GTTCGGA6300.074.543852
TCGGGAA19700.074.160263
ATCCGGA9350.074.072742
GAACCGG4950.072.8389361
TTCGGAG9050.072.3297963
GATCCGG9950.071.519331
AAAGCGG17750.071.071268
CGGAGAG19850.070.971295
Produced by FastQC (version 0.11.2)