FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5005159
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT349140.6975602573264905Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC185800.090.373239
ATCGGGA297650.088.9096152
TCGGGAG267850.086.56113
GAGAGCG225300.086.43827
AGAGCGG204450.085.0251858
GATCGGG316800.084.7122041
GAGCGTC378600.082.965849
TCCGGAG36950.082.140983
CGGGAGA276100.081.346654
GGAGAGC247650.080.10216
AGAGCGT399400.079.805478
ATTGGGA121650.079.102532
AAGAGCG385200.078.968277
CGGAAGA401250.078.773384
GAGGGGC203800.078.761539
GGATCGG55250.078.466121
TCGGAAG411100.078.258723
GAAGAGC394850.077.503966
TCCGGAA27100.076.472723
ATCCGGA52300.076.444522
Produced by FastQC (version 0.11.2)