FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3086871
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA45830.1484674934585864No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC41200.13346848637341827No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT34400.11143970706906767Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC12800.073.358219
AGAGCGG15750.062.928178
GATCGGG27350.050.127371
CGGGAGA23400.048.0392724
TCGGGAG23950.045.153693
ATCGGGA28250.045.0025022
GAGAGCG23350.044.8832977
GGATCGG7100.043.4299551
TTGTGTT267050.043.1310921
GGCGCCG14800.038.35472556-57
GAGGGGC23400.036.6822669
TCTCGGG14150.036.26426348-49
TCGGGAA6450.036.0329863
CGCCGGA8450.035.97937458-59
ATTGGGA18150.035.5453342
AAGGGGC17700.033.759099
GAATCGG3800.033.706571
CGGAGAG10350.033.4538765
GATTGGG22600.033.3751371
GATCTCG22100.033.32406646-47
Produced by FastQC (version 0.11.2)