FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8483560
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT197670.2330035975463131Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC48900.086.694479
CGGGAGA74400.081.079844
ATTCGGA10850.080.870372
AGAGCGG57050.077.132018
GATCGGG95450.075.586071
AAGCGGC16200.073.471959
ATCGGGA95700.073.151272
GAGAGCG76550.072.16447
CGGAAAG22750.072.126265
TTCGGAG9900.068.501413
GATCCGG14200.066.140161
TCGGGAA20700.065.293993
AAAGCGG19950.063.461548
GAACCGG6350.057.5182151
AAGCGTC8350.055.0868649
TCGGAAA20750.055.0804674
AAGGGGC44700.053.5730869
TCGGGAG115200.053.557893
GTTCGGA8850.052.5206642
TTCGGAA13300.052.4160583
Produced by FastQC (version 0.11.2)