##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_30484-1.34200000035803.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8483560 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61075043967391 33.0 33.0 33.0 27.0 33.0 2 31.379912324543 33.0 33.0 33.0 27.0 33.0 3 31.268710777079434 33.0 33.0 33.0 27.0 33.0 4 34.84510370646285 37.0 37.0 37.0 33.0 37.0 5 34.87118674235816 37.0 37.0 37.0 33.0 37.0 6 35.19319648826672 37.0 37.0 37.0 33.0 37.0 7 34.79842353917459 37.0 37.0 37.0 33.0 37.0 8 34.95049554668088 37.0 37.0 37.0 33.0 37.0 9 34.64136577097351 37.0 37.0 37.0 33.0 37.0 10-11 34.611542736775604 37.0 37.0 37.0 33.0 37.0 12-13 34.58514314745225 37.0 37.0 37.0 30.0 37.0 14-15 36.17842250187421 40.0 37.0 40.0 30.0 40.0 16-17 36.1264608254082 40.0 37.0 40.0 27.0 40.0 18-19 36.14669696448189 40.0 37.0 40.0 27.0 40.0 20-21 36.10763105347283 40.0 37.0 40.0 27.0 40.0 22-23 36.489256514953624 40.0 37.0 40.0 33.0 40.0 24-25 35.98825345727501 40.0 37.0 40.0 27.0 40.0 26-27 35.90606231346274 40.0 37.0 40.0 27.0 40.0 28-29 35.6629575319795 40.0 37.0 40.0 27.0 40.0 30-31 35.68273389944787 40.0 37.0 40.0 27.0 40.0 32-33 35.51948285861124 37.0 37.0 40.0 27.0 40.0 34-35 35.64137920872841 37.0 37.0 40.0 27.0 40.0 36-37 35.3907123306725 37.0 37.0 40.0 27.0 40.0 38-39 35.33600929326839 37.0 37.0 40.0 27.0 40.0 40-41 35.56174418522413 37.0 37.0 40.0 27.0 40.0 42-43 35.24491687451966 37.0 37.0 40.0 24.5 40.0 44-45 34.9350290444106 37.0 37.0 40.0 22.0 40.0 46-47 34.77512276685731 37.0 37.0 40.0 22.0 40.0 48-49 34.64168503552754 37.0 35.0 40.0 22.0 40.0 50-51 33.60543686848446 37.0 33.0 38.5 22.0 38.5 52-53 34.05024871634078 37.0 33.0 38.5 22.0 40.0 54-55 34.68310090339433 37.0 33.0 40.0 22.0 40.0 56-57 34.8488120553164 37.0 33.0 40.0 22.0 40.0 58-59 34.47229747888858 37.0 33.0 40.0 22.0 40.0 60-61 34.26305631126555 37.0 33.0 40.0 22.0 40.0 62-63 33.95479274031185 37.0 33.0 40.0 22.0 40.0 64-65 33.699782756295704 37.0 33.0 38.5 22.0 40.0 66-67 33.50203434643004 37.0 33.0 37.0 22.0 40.0 68-69 33.411917520474894 37.0 33.0 37.0 22.0 40.0 70-71 33.06049470976807 37.0 33.0 37.0 22.0 40.0 72-73 33.0671994422153 37.0 33.0 37.0 22.0 37.0 74-75 32.96810389742042 37.0 33.0 37.0 22.0 37.0 76-77 32.68636450971054 37.0 33.0 37.0 22.0 37.0 78-79 32.3290586145439 37.0 33.0 37.0 22.0 37.0 80-81 31.931644557237767 37.0 33.0 37.0 22.0 37.0 82-83 31.630226284720095 33.0 33.0 37.0 15.0 37.0 84-85 31.39490508701536 33.0 33.0 37.0 15.0 37.0 86-87 31.233055934065415 33.0 33.0 37.0 15.0 37.0 88-89 31.069691851062522 33.0 33.0 37.0 15.0 37.0 90-91 30.915051110618656 33.0 33.0 37.0 15.0 37.0 92-93 30.76092454111246 33.0 30.0 37.0 15.0 37.0 94-95 30.59586317536506 33.0 27.0 37.0 15.0 37.0 96-97 30.456754063152733 33.0 27.0 37.0 15.0 37.0 98-99 30.28985331629646 33.0 27.0 37.0 15.0 37.0 100-101 28.971278567016675 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3160902080265089 2306 2 0.2659696554060744 2306 3 0.301951792031268 2306 4 0.4243919390901496 2306 5 0.4083136479458318 2306 6 0.2765780929287729 2306 7 0.6624234026738307 2306 8 0.23390920584992614 2306 9 0.3786811368441363 2306 10-11 0.36596739840652504 2306 12-13 0.4144071184347524 2306 14-15 0.6940707497501677 2306 16-17 0.6257460725963426 2306 18-19 0.6056519413703896 2306 20-21 0.5922184405493383 2306 22-23 0.5722995335182048 2306 24-25 0.8354311696198451 2306 26-27 0.6011409111521218 2306 28-29 0.6581348120993127 2306 30-31 0.6981438712492078 2306 32-33 0.7099330278155946 2306 34-35 0.6167077224569582 2306 36-37 0.6438786953471549 2306 38-39 0.6184001858173289 2306 40-41 0.38713212727947877 2306 42-43 0.6299387570079134 2306 44-45 0.6994260118353566 2306 46-47 0.5921147662091926 2306 48-49 0.6628292030733647 2306 50-51 0.5459689033323585 2306 52-53 0.6430393235771135 2306 54-55 0.7251031530998944 2306 56-57 0.6587955449799452 2306 58-59 0.8720471302521773 2306 60-61 0.8660499497807663 2306 62-63 0.6996040380442352 2306 64-65 0.6805656119546271 2306 66-67 0.5960241582104828 2306 68-69 0.5518613507302632 2306 70-71 0.6299362575441307 2306 72-73 0.3854792828459992 2306 74-75 0.48656714586559957 2306 76-77 0.49562552933581827 2306 78-79 0.49934311209757 2306 80-81 0.6244711304334558 2306 82-83 0.5690660662782783 2306 84-85 0.6235012924101824 2306 86-87 0.6547946029231433 2306 88-89 0.6202554886277412 2306 90-91 0.530986015372946 2306 92-93 0.3824828447356978 2306 94-95 0.4309227785033052 2306 96-97 0.4664076735691225 2306 98-99 0.5947186697420364 2306 100-101 0.5142264069175795 2307 1 0.31164750486152215 2307 2 0.2314660089876348 2307 3 0.2543743753188821 2307 4 0.3236931825003069 2307 5 0.3329140804215811 2307 6 0.24578127650741521 2307 7 0.5391694109151928 2307 8 0.26874996760910363 2307 9 0.40101154319313537 2307 10-11 0.4172821466367793 2307 12-13 0.41150638863950917 2307 14-15 0.6657478496527887 2307 16-17 0.6025340955635414 2307 18-19 0.5942731795921006 2307 20-21 0.6317941637558917 2307 22-23 0.5223701266343497 2307 24-25 0.7318844534234827 2307 26-27 0.5381896728846982 2307 28-29 0.5134251266291372 2307 30-31 0.6553971568688652 2307 32-33 0.5427996569745588 2307 34-35 0.4495983627711624 2307 36-37 0.6854122549011663 2307 38-39 0.5231240177265803 2307 40-41 0.32598441132383726 2307 42-43 0.6053003542951885 2307 44-45 0.4991987946889225 2307 46-47 0.3566600350540483 2307 48-49 0.43871071061198563 2307 50-51 0.35024929300622887 2307 52-53 0.42319831770986127 2307 54-55 0.5096803117003574 2307 56-57 0.5035652967049202 2307 58-59 0.46450966094033674 2307 60-61 0.41755050470701605 2307 62-63 0.4161718055013992 2307 64-65 0.5198430756885912 2307 66-67 0.4679985807989553 2307 68-69 0.31745366260773267 2307 70-71 0.3659963351810589 2307 72-73 0.21023182007535723 2307 74-75 0.3994216473129626 2307 76-77 0.4467437249988464 2307 78-79 0.5032203381690579 2307 80-81 0.4509705831165931 2307 82-83 0.4456027522381696 2307 84-85 0.5424637713512652 2307 86-87 0.42229591354535856 2307 88-89 0.5149789141697738 2307 90-91 0.4475746351276584 2307 92-93 0.5043478503121328 2307 94-95 0.5633865669795419 2307 96-97 0.5876083918319779 2307 98-99 0.3918841001113016 2307 100-101 0.4180047076689064 2201 1 0.28334729011422866 2201 2 0.28765229623650157 2201 3 0.373474671051202 2201 4 0.4688964248120442 2201 5 0.3874767862460402 2201 6 0.39593562032823115 2201 7 0.5870681162819338 2201 8 0.45917413025375 2201 9 0.5133319825097971 2201 10-11 0.4414163980096788 2201 12-13 0.4359694026950294 2201 14-15 0.7063535651467987 2201 16-17 0.6593306241081152 2201 18-19 0.6255615506359291 2201 20-21 0.6535865838370469 2201 22-23 0.5797286279148324 2201 24-25 0.7514056258857806 2201 26-27 0.6284378115203282 2201 28-29 0.5592142690710418 2201 30-31 0.6583941735779533 2201 32-33 0.5493137580857024 2201 34-35 0.5163688479807647 2201 36-37 0.619998711197006 2201 38-39 0.5252558957645022 2201 40-41 0.4617839593999875 2201 42-43 0.6421355148091976 2201 44-45 0.7220726702119649 2201 46-47 0.6199714494483999 2201 48-49 0.6089989365505133 2201 50-51 0.5755836094981603 2201 52-53 0.6631572230879357 2201 54-55 0.8000888366442709 2201 56-57 0.7597474890449334 2201 58-59 0.8652955124737787 2201 60-61 0.697053802250089 2201 62-63 0.6793832869980534 2201 64-65 0.692526195518326 2201 66-67 0.7005931992326282 2201 68-69 0.6229704179902882 2201 70-71 0.645798118763544 2201 72-73 0.5310117846422742 2201 74-75 0.5698579320292652 2201 76-77 0.5782122863004204 2201 78-79 0.7641777364209119 2201 80-81 0.7823964403686432 2201 82-83 0.6398285883165435 2201 84-85 0.63580634843008 2201 86-87 0.6302376111747172 2201 88-89 0.6267517628694534 2201 90-91 0.6747649726266189 2201 92-93 0.6569520768002626 2201 94-95 0.5489177273097745 2201 96-97 0.6974775216838225 2201 98-99 0.5188347070923669 2201 100-101 0.5699977654429347 1101 1 0.2553839314070956 1101 2 0.05739077102203893 1101 3 0.19626721944223746 1101 4 0.2824537237575342 1101 5 0.2718740810283933 1101 6 0.2706346771505963 1101 7 0.35398072469114084 1101 8 0.24050388812393209 1101 9 0.2966215295418877 1101 10-11 0.2731944846351553 1101 12-13 0.2597215960304382 1101 14-15 0.37892476339652603 1101 16-17 0.40240371101598527 1101 18-19 0.43700576012111725 1101 20-21 0.3444553871619931 1101 22-23 0.3277357176033462 1101 24-25 0.43876644340503645 1101 26-27 0.39084303608095183 1101 28-29 0.36692390008895615 1101 30-31 0.449463968054431 1101 32-33 0.4239414008466085 1101 34-35 0.3266186891291838 1101 36-37 0.4156604259655623 1101 38-39 0.3419674679944009 1101 40-41 0.2786824612485077 1101 42-43 0.31732811471201927 1101 44-45 0.2574317133793329 1101 46-47 0.23376276420687248 1101 48-49 0.3329653463401172 1101 50-51 0.23778160334325804 1101 52-53 0.27384450270105987 1101 54-55 0.5107668405344086 1101 56-57 0.5111317138061153 1101 58-59 0.5006735316347743 1101 60-61 0.49692920394614504 1101 62-63 0.43086282914013196 1101 64-65 0.534714550845159 1101 66-67 0.4934281213390719 1101 68-69 0.395156847414583 1101 70-71 0.4368913662816496 1101 72-73 0.4172887834608616 1101 74-75 0.420936212312796 1101 76-77 0.47328696744904875 1101 78-79 0.49097267565849734 1101 80-81 0.5213551845000737 1101 82-83 0.42931186107464825 1101 84-85 0.4028413294843176 1101 86-87 0.5078537349285561 1101 88-89 0.526880259292767 1101 90-91 0.3507265569818614 1101 92-93 0.2685044044213676 1101 94-95 0.3138906418126979 1101 96-97 0.30106666041686125 1101 98-99 0.2847221744327513 1101 100-101 0.4910044219641989 2304 1 0.2769429379130237 2304 2 0.14868053670485892 2304 3 0.2186299839894872 2304 4 0.23998752215410235 2304 5 0.2060552361968888 2304 6 0.24035504791594775 2304 7 0.2295218384655513 2304 8 0.2987050671685054 2304 9 0.19647916379241082 2304 10-11 0.26085043211993764 2304 12-13 0.22093126489549775 2304 14-15 0.35215063322168305 2304 16-17 0.3443375983476642 2304 18-19 0.29737061105836204 2304 20-21 0.26702628235484127 2304 22-23 0.34939246663277856 2304 24-25 0.5514652391349344 2304 26-27 0.4451641276040732 2304 28-29 0.47554503146067617 2304 30-31 0.43632746775631404 2304 32-33 0.34664636516711766 2304 34-35 0.45671277175035385 2304 36-37 0.36280070496020045 2304 38-39 0.33359775515408074 2304 40-41 0.460215428864025 2304 42-43 0.4004523481227622 2304 44-45 0.3360474710405299 2304 46-47 0.4023241788735703 2304 48-49 0.38881474565229723 2304 50-51 0.31193266690036126 2304 52-53 0.3513350477506805 2304 54-55 0.5468880966230145 2304 56-57 0.7073440347443594 2304 58-59 0.6651514322289813 2304 60-61 0.5297985175100237 2304 62-63 0.6434940941142102 2304 64-65 0.617320494704586 2304 66-67 0.5761684383653716 2304 68-69 0.5944227435388854 2304 70-71 0.6007356978197222 2304 72-73 0.6166473020884666 2304 74-75 0.7668464800305088 2304 76-77 0.6153703861261874 2304 78-79 0.6247925519855642 2304 80-81 0.6473898937616198 2304 82-83 0.6446261017675035 2304 84-85 0.47156467299907234 2304 86-87 0.5782408417788041 2304 88-89 0.5818023390308724 2304 90-91 0.5615906488061739 2304 92-93 0.5973991302143666 2304 94-95 0.6524517590074304 2304 96-97 0.5759638532082931 2304 98-99 0.5734643396768924 2304 100-101 0.522603751608834 2202 1 0.2246271034922671 2202 2 0.1785718241619385 2202 3 0.1931036045082024 2202 4 0.2753355468033192 2202 5 0.27938050247436763 2202 6 0.25062986544202204 2202 7 0.27894102614452976 2202 8 0.2306848855773751 2202 9 0.2422692732400904 2202 10-11 0.3133233928179848 2202 12-13 0.3333567885348643 2202 14-15 0.45153591255318304 2202 16-17 0.3292041257532716 2202 18-19 0.33953629874439883 2202 20-21 0.47199042958178694 2202 22-23 0.31146867224277486 2202 24-25 0.4539198030139673 2202 26-27 0.33526731432629475 2202 28-29 0.45110878206824623 2202 30-31 0.4141410567370727 2202 32-33 0.4097360874223597 2202 34-35 0.34612983358425 2202 36-37 0.45000430480484255 2202 38-39 0.4813134363221394 2202 40-41 0.3950251656216466 2202 42-43 0.4329783908541813 2202 44-45 0.4033281342749504 2202 46-47 0.4340699349423929 2202 48-49 0.4105374346673756 2202 50-51 0.47846965859427115 2202 52-53 0.5219916708388865 2202 54-55 0.6430400546276331 2202 56-57 0.6000688353877663 2202 58-59 0.6063752892436582 2202 60-61 0.540403352912854 2202 62-63 0.5698588983787047 2202 64-65 0.5416119870377258 2202 66-67 0.5760201626066177 2202 68-69 0.599922023442204 2202 70-71 0.5449731725239602 2202 72-73 0.508387705690609 2202 74-75 0.4407562117531185 2202 76-77 0.6008185958491126 2202 78-79 0.6251528853079193 2202 80-81 0.6087693172975577 2202 82-83 0.612912618367087 2202 84-85 0.6762183960852894 2202 86-87 0.5927585957361536 2202 88-89 0.5803205062848491 2202 90-91 0.5264864489523049 2202 92-93 0.5813320730939431 2202 94-95 0.5722646523782977 2202 96-97 0.5880528442149817 2202 98-99 0.5764830870108497 2202 100-101 0.6326505075109523 1102 1 0.060521789312588226 1102 2 0.02811931717814531 1102 3 0.1082817183245588 1102 4 0.019809757991794186 1102 5 0.08510573781566677 1102 6 0.06189523025032173 1102 7 0.05117486465710641 1102 8 0.1498761186135269 1102 9 0.05818744395039488 1102 10-11 0.11900299144927828 1102 12-13 0.07606464609269636 1102 14-15 0.059115664208974295 1102 16-17 0.05896353951302302 1102 18-19 0.055153409137552956 1102 20-21 0.1283975433232385 1102 22-23 0.09015580572042126 1102 24-25 0.06317794149768474 1102 26-27 0.2121543774592567 1102 28-29 0.09964791674662621 1102 30-31 0.13330986048487858 1102 32-33 -0.010458668023822781 1102 34-35 0.11241271340064429 1102 36-37 0.19338156367373216 1102 38-39 0.054650533089144915 1102 40-41 0.10034522712760463 1102 42-43 0.08616663844620831 1102 44-45 0.14377222591770078 1102 46-47 0.18931515547866695 1102 48-49 0.22315720070551492 1102 50-51 0.18858973336983098 1102 52-53 0.21220209066571272 1102 54-55 0.32057082809286186 1102 56-57 0.4408439176255001 1102 58-59 0.45825835029143747 1102 60-61 0.4311596269675917 1102 62-63 0.38847761399077996 1102 64-65 0.42045256912313533 1102 66-67 0.3951569299051485 1102 68-69 0.35294478382357397 1102 70-71 0.28621268533559174 1102 72-73 0.379184222356173 1102 74-75 0.3256982215927451 1102 76-77 0.3812537438108876 1102 78-79 0.3697613110144857 1102 80-81 0.34069034226553896 1102 82-83 0.2101211019538276 1102 84-85 0.2897394022343107 1102 86-87 0.34952102347758185 1102 88-89 0.32235150467194273 1102 90-91 0.2984964258793035 1102 92-93 0.25846176645981345 1102 94-95 0.27863152782190426 1102 96-97 0.3216479789732958 1102 98-99 0.4042421105178775 1102 100-101 0.32876982550244094 2305 1 0.2708272531814835 2305 2 0.29019314452563805 2305 3 0.31546578287398575 2305 4 0.29921837241239047 2305 5 0.3178165732927667 2305 6 0.3530450989242411 2305 7 0.39288347631480747 2305 8 0.3754910941896128 2305 9 0.33369440778403003 2305 10-11 0.33341370422331806 2305 12-13 0.3839189994207217 2305 14-15 0.3175997238507833 2305 16-17 0.3549932627069907 2305 18-19 0.39373713631034235 2305 20-21 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-1.3252919444907576 1213 60-61 -0.9400231246012751 1213 62-63 -0.9267201800004585 1213 64-65 -0.7529911092885513 1213 66-67 -0.6838393658987485 1213 68-69 -0.5196024280785423 1213 70-71 -0.6568974108916947 1213 72-73 -0.5382600430388749 1213 74-75 -0.5700634940089486 1213 76-77 -0.816002044086833 1213 78-79 -0.7951724378660501 1213 80-81 -0.8463551352274052 1213 82-83 -0.7978343709922626 1213 84-85 -0.7408003099652873 1213 86-87 -0.8982324990312627 1213 88-89 -0.6452071745895438 1213 90-91 -0.6591739539115835 1213 92-93 -0.6314300851853325 1213 94-95 -0.635771139916784 1213 96-97 -0.5766803075369182 1213 98-99 -0.7557066729120478 1213 100-101 -1.2465708512913096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4022.0 3 390.0 4 514.0 5 603.0 6 1312.0 7 6868.0 8 25963.0 9 67785.0 10 108747.0 11 92175.0 12 61889.0 13 44416.0 14 34128.0 15 28878.0 16 26572.0 17 26757.0 18 27820.0 19 29771.0 20 32755.0 21 36831.0 22 40726.0 23 44647.0 24 50907.0 25 59106.0 26 71111.0 27 85239.0 28 104018.0 29 125409.0 30 153195.0 31 196624.0 32 265673.0 33 375859.0 34 561513.0 35 910277.0 36 1627513.0 37 2472620.0 38 679758.0 39 1169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.57487170374708 27.864606055382424 17.286489237164915 17.274033003705583 2 31.969711351608147 27.404218725428663 25.301172245968118 15.32489767699507 3 34.244781708432825 22.5322227792932 24.577582047585192 18.645413464688783 4 30.55175410603755 20.964902301042702 26.366928600831592 22.11641499208816 5 33.70365596667368 19.83431499814337 25.750542175941433 20.711486859241518 6 32.180799697781765 19.748072768484302 27.653830261961822 20.41729727177211 7 33.42497727392363 21.04956350117779 23.166647113546347 22.35881211135223 8 28.96291183988251 26.716133754448347 22.47580598906363 21.845148416605515 9 28.943850551355272 24.522800727115843 25.286357326173576 21.246991395355312 10-11 24.66180575180072 29.67684689888066 28.208118266660453 17.45322908265817 12-13 25.11637447938354 26.9183435922276 28.67489006978692 19.29039185860194 14-15 23.759509810088822 26.994494604660634 29.657471573396833 19.588524011853707 16-17 25.22598437351012 26.850854443853866 29.72157408185403 18.201587100781985 18-19 24.982460943515683 26.620269543878322 30.150974546093938 18.246294966512057 20-21 24.307547148437575 28.410916168388223 29.068240153533324 18.21329652964088 22-23 26.07071414144162 27.19828322016829 28.134792802322195 18.596209836067896 24-25 23.74966326276015 28.95368578734493 28.43638281946481 18.86026813043011 26-27 23.01767776407363 29.819669723056936 28.658519248224344 18.50413326464509 28-29 23.51796591858101 29.384445304981753 28.550511682780044 18.547077093657194 30-31 24.60230412797529 28.03862517859603 29.044220931466146 18.314849761962538 32-33 22.280149656925417 29.500472885392732 29.84522864400224 18.37414881367961 34-35 24.893959676013345 28.30746227198449 28.71622208629203 18.082355965710132 36-37 22.76639293641442 28.782359569194277 30.218093003514323 18.23315449087698 38-39 22.765036721786288 29.671232876712327 28.96492145823174 18.598808943269642 40-41 25.309642570460817 27.415040053034044 28.186418028596705 19.088899347908438 42-43 25.06555890940301 27.332856641620346 28.555493414658333 19.046091034318312 44-45 23.20669354413045 28.300711569712018 29.39391061069145 19.098684275466084 46-47 23.611923463740098 28.823397221940244 28.524949393050647 19.039729921269014 48-49 21.56017027503739 28.9922681908697 29.634122216952512 19.813439317140393 50-51 21.248611000667783 28.148287632459322 30.029569907571496 20.573531459301396 52-53 24.301705176403452 27.81999505958474 27.922817895560474 19.955481868451333 54-55 24.947126506899725 27.77826347482723 28.01132712512015 19.263282893152894 56-57 25.097045358854707 27.434954395686834 28.117360829725257 19.350639415733202 58-59 24.259798305938247 27.68122888822335 28.10002670951224 19.958946096326162 60-61 21.96782752451266 27.858862414082314 27.87425947806022 22.29905058334481 62-63 23.378480628556076 27.87214543214176 28.101091263543864 20.648282675758303 64-65 21.7849945220273 28.538047749243322 29.49802145733875 20.17893627139062 66-67 21.316214092453926 28.176431652937868 28.848108300515158 21.659245954093052 68-69 20.97990986356118 27.76383302581717 31.420956367381407 19.83530074324024 70-71 20.689630313039046 30.280662565015188 29.60432557847923 19.425381543466536 72-73 20.734279524554715 31.949655954189073 27.7351472095375 19.58091731171871 74-75 20.915624831958173 31.876738401937732 27.55963379734052 19.648002968763574 76-77 21.049431446190063 31.554145562065845 27.645601064546398 19.750821927197695 78-79 20.934899373353158 31.296233772940585 27.98478567122779 19.78408118247847 80-81 20.601823687012303 31.538934690088617 27.74274722853219 20.116494394366892 82-83 20.564272173835832 31.256749005968153 27.695750879639412 20.483227940556606 84-85 20.80734319854379 30.831422409208713 27.95506554075118 20.40616885149632 86-87 20.865979632528106 30.471692753853137 28.526543679472926 20.13578393414583 88-89 21.160177540588247 30.752903999842037 28.15004896802966 19.93686949154005 90-91 21.414362332877424 30.352900540526544 27.734284565804746 20.498452560791282 92-93 21.2338149529317 30.420838407658007 27.591805879660974 20.753540759749324 94-95 20.903273297259457 30.402440109575906 27.584344225573883 21.109942367590754 96-97 20.867608316174056 30.78549961939281 27.642900820614276 20.703991243818862 98-99 21.306895514431723 30.41077472517406 27.518224708064853 20.764105052329366 100-101 21.55976928116727 30.368982763224317 27.064056874303294 21.007191081305116 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3243.0 1 1887.0 2 436.5 3 312.0 4 282.5 5 327.5 6 492.0 7 770.0 8 1106.0 9 1569.5 10 2068.5 11 2430.5 12 2690.0 13 2736.0 14 2552.0 15 2332.5 16 2061.0 17 1802.5 18 1786.0 19 1975.5 20 2277.5 21 2840.5 22 3780.5 23 5205.5 24 7298.5 25 9896.5 26 13519.5 27 18409.0 28 24157.0 29 30655.5 30 38471.0 31 47940.0 32 64333.5 33 93063.5 34 135140.5 35 196372.0 36 268107.5 37 348863.0 38 434275.5 39 484687.0 40 521862.5 41 528873.5 42 498120.5 43 494001.5 44 489473.0 45 485280.5 46 506151.5 47 485464.5 48 408810.5 49 334935.5 50 277752.5 51 233125.5 52 194870.0 53 160464.0 54 134226.5 55 111144.0 56 86486.0 57 64184.5 58 48158.5 59 37219.0 60 29049.0 61 22089.5 62 16465.0 63 12323.5 64 9426.5 65 7289.5 66 5606.0 67 4284.5 68 3283.5 69 2540.0 70 1929.0 71 1501.5 72 1174.0 73 944.0 74 771.0 75 600.5 76 484.0 77 407.5 78 325.0 79 262.0 80 247.0 81 212.5 82 171.0 83 146.5 84 143.0 85 129.0 86 98.5 87 88.5 88 84.5 89 65.5 90 51.0 91 47.0 92 39.5 93 33.0 94 31.5 95 29.0 96 19.5 97 14.0 98 11.5 99 4.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16390524732541525 2 0.15705670732569818 3 0.12579624591562977 4 0.12004394381603949 5 0.13223222326476147 6 0.15158730532936646 7 0.12864882195682 8 0.12002036880743461 9 0.11235849101084922 10-11 0.09989909896317112 12-13 0.12862524694821514 14-15 0.12493575810155172 16-17 0.1148574419229663 18-19 0.11333685386795166 20-21 0.1066533389284687 22-23 0.11812847436689314 24-25 0.4106294998797675 26-27 0.2953064515368548 28-29 0.3862470472301722 30-31 0.22309030642796182 32-33 0.26177100179641566 34-35 0.2750614128974157 36-37 0.2715958866324986 38-39 0.29097454370570847 40-41 0.2839432973893036 42-43 0.33900272998599645 44-45 0.2864893983186304 46-47 0.3120211326377134 48-49 0.3114140761661378 50-51 0.31216258268934266 52-53 0.31568115272362074 54-55 0.29382122599474747 56-57 0.30549674900631335 58-59 0.3052845739288695 60-61 0.28472716642541573 62-63 0.28192174040143525 64-65 0.29094507494495236 66-67 0.2897250682496499 68-69 0.2874265049106743 70-71 0.26134665164152787 72-73 0.2521052482684156 74-75 0.2607985326914644 76-77 0.25626034353502536 78-79 0.2538674801616303 80-81 0.26279651467072784 82-83 0.30450659864490853 84-85 0.30959290675141093 86-87 0.29959710310294263 88-89 0.3064868993677183 90-91 0.2821751717439377 92-93 0.2671519975104791 94-95 0.2532073799206937 96-97 0.2525295984233034 98-99 0.27352903733809864 100-101 0.27369406239833277 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8483560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.829934871781255 #Duplication Level Percentage of deduplicated Percentage of total 1 89.43450851210868 45.45950242960251 2 5.109550918003926 5.1943628077237936 3 1.6773646768977475 2.5578101184881676 4 0.8982279271085974 1.8262746813978035 5 0.5495142038550355 1.396588559653509 6 0.30162287310016733 0.919888259731263 7 0.23901984750093028 0.8504554297074763 8 0.22644718433529407 0.9208236505328994 9 0.14785497534641753 0.6763912890594505 >10 1.118638636587433 11.761889701028082 >50 0.1683110475298862 6.0605817502163015 >100 0.10910629255615988 10.627568811092928 >500 0.011364373174178958 4.052207140226004 >1k 0.008352012937279692 7.194489772329902 >5k 9.327732520996273E-5 0.2676440555214268 >10k+ 2.3241632906391107E-5 0.23352154368855815 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 19767 0.2330035975463131 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0030765386229365975 0.0 2.357500860487814E-5 2 0.0033122887089853787 0.0 2.357500860487814E-5 3 0.0036423388294536726 0.0 4.715001720975628E-5 4 0.003830938898292698 0.0 4.715001720975628E-5 5 0.004231714044575626 0.0 5.8937521512195355E-5 6 0.0046560641994634324 0.0 5.8937521512195355E-5 7 0.0052925894317951425 0.0 5.8937521512195355E-5 8 0.005999839689941487 0.0 5.8937521512195355E-5 9 0.006624577417970757 0.0 5.8937521512195355E-5 10-11 0.01015493495655126 0.0 7.072502581463442E-5 12-13 0.01367939874298054 0.0 7.661877796585396E-5 14-15 0.018370825455351292 0.0 8.251253011707349E-5 16-17 0.022349108157424476 0.0 8.251253011707349E-5 18-19 0.027706528862883037 0.0 8.251253011707349E-5 20-21 0.03402463116899038 0.0 8.251253011707349E-5 22-23 0.041132496263361136 0.0 9.430003441951256E-5 24-25 0.05001438075524897 0.0 1.001937865707321E-4 26-27 0.05942080918859535 0.0 1.0608753872195164E-4 28-29 0.07063661953236613 0.0 1.0608753872195164E-4 30-31 0.10467303820565894 0.0 1.0608753872195164E-4 32-33 0.11863533705189802 0.0 1.0608753872195164E-4 34-35 0.13199647317871271 0.0 1.0608753872195164E-4 36-37 0.14692534737775179 0.0 1.0608753872195164E-4 38-39 0.16293277822046404 0.0 1.0608753872195164E-4 40-41 0.17967692808207875 0.0 1.0608753872195164E-4 42-43 0.19742890956155199 0.0 1.0608753872195164E-4 44-45 0.2168016728826106 0.0 1.0608753872195164E-4 46-47 0.23690526147042043 0.0 1.0608753872195164E-4 48-49 0.25786933787230837 0.0 1.1198129087317118E-4 50-51 0.279257764429084 0.0 1.1787504302439071E-4 52-53 0.3016363413472646 0.0 1.1787504302439071E-4 54-55 0.3248105748058598 0.0 1.1787504302439071E-4 56-57 0.3492166024640599 0.0 1.2376879517561024E-4 58-59 0.3749133618433771 0.0 1.2966254732682978E-4 60-61 0.4004627774189138 0.0 1.2966254732682978E-4 62-63 0.4291712441475041 0.0 1.2966254732682978E-4 64-65 0.4586341111514506 0.0 1.2966254732682978E-4 66-67 0.48945254115017756 1.178750430243907E-5 1.2966254732682978E-4 68-69 0.5228936908561972 1.178750430243907E-5 1.2966254732682978E-4 70-71 0.5588573664829388 1.178750430243907E-5 1.2966254732682978E-4 72-73 0.5973553555347048 1.178750430243907E-5 1.2966254732682978E-4 74-75 0.6385998330889391 1.178750430243907E-5 1.2966254732682978E-4 76-77 0.6821075114692416 1.178750430243907E-5 1.4145005162926884E-4 78-79 0.7305011103829053 1.178750430243907E-5 1.6502506023414697E-4 80-81 0.7835213047352763 1.178750430243907E-5 1.7091881238536652E-4 82-83 0.840885194423096 1.178750430243907E-5 1.7681256453658606E-4 84-85 0.9023923918732231 1.178750430243907E-5 1.7681256453658606E-4 86-87 0.9693513100632283 1.178750430243907E-5 1.7681256453658606E-4 88-89 1.0425458180292235 1.178750430243907E-5 1.8270631668780557E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4890 0.0 86.69447 9 CGGGAGA 7440 0.0 81.07984 4 ATTCGGA 1085 0.0 80.87037 2 AGAGCGG 5705 0.0 77.13201 8 GATCGGG 9545 0.0 75.58607 1 AAGCGGC 1620 0.0 73.47195 9 ATCGGGA 9570 0.0 73.15127 2 GAGAGCG 7655 0.0 72.1644 7 CGGAAAG 2275 0.0 72.12626 5 TTCGGAG 990 0.0 68.50141 3 GATCCGG 1420 0.0 66.14016 1 TCGGGAA 2070 0.0 65.29399 3 AAAGCGG 1995 0.0 63.46154 8 GAACCGG 635 0.0 57.518215 1 AAGCGTC 835 0.0 55.086864 9 TCGGAAA 2075 0.0 55.080467 4 AAGGGGC 4470 0.0 53.573086 9 TCGGGAG 11520 0.0 53.55789 3 GTTCGGA 885 0.0 52.520664 2 TTCGGAA 1330 0.0 52.416058 3 >>END_MODULE