##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_30423-1.34200000035748.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2641641 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.912175045738614 33.0 33.0 33.0 27.0 33.0 2 30.350922778681888 33.0 33.0 33.0 22.0 33.0 3 30.161921320875926 33.0 33.0 33.0 22.0 33.0 4 33.44747980516656 37.0 37.0 37.0 15.0 37.0 5 33.56169895909399 37.0 37.0 37.0 15.0 37.0 6 34.332997178647666 37.0 37.0 37.0 27.0 37.0 7 33.50346167401248 37.0 37.0 37.0 15.0 37.0 8 33.926676637741465 37.0 37.0 37.0 22.0 37.0 9 33.22705810517024 37.0 37.0 37.0 15.0 37.0 10-11 33.26209579575726 37.0 37.0 37.0 15.0 37.0 12-13 33.21438265078412 37.0 37.0 37.0 15.0 37.0 14-15 34.60737265207498 40.0 37.0 40.0 15.0 40.0 16-17 34.48481530987746 37.0 37.0 40.0 15.0 40.0 18-19 34.63817282514921 38.5 37.0 40.0 15.0 40.0 20-21 34.602796897837365 40.0 37.0 40.0 15.0 40.0 22-23 35.54902956154905 37.0 37.0 40.0 24.5 40.0 24-25 34.66163362091972 37.0 37.0 40.0 15.0 40.0 26-27 34.54845018683462 37.0 37.0 40.0 18.5 40.0 28-29 34.1567442358746 37.0 37.0 40.0 15.0 40.0 30-31 34.19724822562945 37.0 37.0 40.0 15.0 40.0 32-33 33.980358231871776 37.0 37.0 40.0 15.0 40.0 34-35 34.32437905074914 37.0 35.0 40.0 18.5 40.0 36-37 33.80331354639029 37.0 33.0 40.0 15.0 40.0 38-39 33.76047521218818 37.0 33.0 40.0 15.0 40.0 40-41 34.42864473257343 37.0 33.0 40.0 18.5 40.0 42-43 33.89770563070455 37.0 33.0 40.0 15.0 40.0 44-45 33.32333083867187 37.0 33.0 40.0 10.5 40.0 46-47 33.13657060137997 37.0 33.0 40.0 6.0 40.0 48-49 33.02832822476635 37.0 33.0 40.0 10.5 40.0 50-51 32.10924137685628 37.0 33.0 38.5 15.0 38.5 52-53 32.61055438645902 37.0 33.0 38.5 15.0 40.0 54-55 33.19265221882913 37.0 33.0 40.0 15.0 40.0 56-57 33.53390430418062 37.0 33.0 40.0 18.5 40.0 58-59 32.898759521070424 37.0 33.0 40.0 10.5 40.0 60-61 32.74645381412539 37.0 33.0 40.0 10.5 40.0 62-63 32.36002715736166 37.0 33.0 38.5 6.0 40.0 64-65 32.11053110547573 37.0 33.0 37.0 6.0 40.0 66-67 31.96570294752391 37.0 33.0 37.0 10.5 40.0 68-69 32.13113515424693 37.0 33.0 37.0 15.0 38.5 70-71 31.70084996409429 37.0 33.0 37.0 15.0 37.0 72-73 32.08240464923129 37.0 33.0 37.0 18.5 37.0 74-75 32.22601632848672 37.0 33.0 37.0 22.0 37.0 76-77 31.86545768331124 35.0 33.0 37.0 22.0 37.0 78-79 31.412931583057652 33.0 33.0 37.0 18.5 37.0 80-81 30.894321749246018 33.0 27.0 37.0 15.0 37.0 82-83 30.388777468247955 33.0 27.0 37.0 15.0 37.0 84-85 30.150693640808875 33.0 27.0 37.0 15.0 37.0 86-87 30.011120360412335 33.0 27.0 37.0 6.0 37.0 88-89 29.936911374407046 33.0 27.0 37.0 6.0 37.0 90-91 29.83868663455784 33.0 27.0 37.0 6.0 37.0 92-93 29.637268084497478 33.0 27.0 37.0 6.0 37.0 94-95 29.420459668819497 33.0 27.0 37.0 6.0 37.0 96-97 29.298315706032728 33.0 27.0 37.0 6.0 37.0 98-99 29.152959656516536 33.0 27.0 37.0 6.0 37.0 100-101 27.887348432281296 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.0623818568907879 2306 2 -0.33579844252795965 2306 3 -0.33150076390121797 2306 4 -0.19004778367511221 2306 5 -0.19713118116362693 2306 6 -0.3585874591745366 2306 7 0.1675235505723407 2306 8 -0.3830934892251747 2306 9 -0.13204124970307163 2306 10-11 -0.15784438424871183 2306 12-13 -0.16757514996506018 2306 14-15 0.08673701594711503 2306 16-17 0.05610188527589344 2306 18-19 0.054084567564054 2306 20-21 0.011649040462643256 2306 22-23 0.08583444979055344 2306 24-25 0.45602671839237274 2306 26-27 0.04189958050289988 2306 28-29 0.07753677627385969 2306 30-31 0.1769895292544561 2306 32-33 0.12915744009104202 2306 34-35 -0.09090436401861979 2306 36-37 0.08633405334358457 2306 38-39 -0.061134343416782144 2306 40-41 -0.28511288108173716 2306 42-43 0.08282062852084948 2306 44-45 0.12168244970100517 2306 46-47 -0.01042205049739664 2306 48-49 0.08004732551307825 2306 50-51 -0.0319596554416961 2306 52-53 -0.04065662249244184 2306 54-55 0.14698655060209376 2306 56-57 0.21418073891013023 2306 58-59 0.4102354898810674 2306 60-61 0.22332799874499187 2306 62-63 0.23482057763254005 2306 64-65 0.27548772997384674 2306 66-67 0.1410702377962494 2306 68-69 0.07845555608343346 2306 70-71 0.12730099409869666 2306 72-73 -0.27584147507235457 2306 74-75 0.0029369261201068753 2306 76-77 0.11439792713408181 2306 78-79 0.17320806015835544 2306 80-81 0.16497823768630937 2306 82-83 0.13543012964484547 2306 84-85 0.18703651714524483 2306 86-87 0.19743888558439693 2306 88-89 0.11228649576943539 2306 90-91 -0.0078382221870541 2306 92-93 0.06280946615078165 2306 94-95 0.04129324788145894 2306 96-97 -0.04763806747682153 2306 98-99 -0.04453968709119849 2306 100-101 0.049929895058479445 2307 1 -0.18648248020485525 2307 2 -0.3628095728749017 2307 3 -0.24328773176925012 2307 4 -0.1974177496728302 2307 5 -0.2651789234344193 2307 6 -0.27838307950563035 2307 7 0.34925492160709126 2307 8 -0.35583530250912077 2307 9 -0.2115600936988713 2307 10-11 -0.14169348587855524 2307 12-13 -0.24356694403351753 2307 14-15 0.15046236978673022 2307 16-17 0.021885493177308035 2307 18-19 0.0978062247083713 2307 20-21 0.01873077402973422 2307 22-23 0.032571475671090866 2307 24-25 0.36224723175720897 2307 26-27 -0.011858238373669394 2307 28-29 0.12196775970461005 2307 30-31 0.4139010318329923 2307 32-33 0.1338105180473903 2307 34-35 -0.12612947812205988 2307 36-37 0.3133557979641637 2307 38-39 -0.037702968355937116 2307 40-41 -0.23617668750666354 2307 42-43 0.2457323433848586 2307 44-45 0.07626891394004787 2307 46-47 0.03885745992837997 2307 48-49 -0.08987621511579391 2307 50-51 -0.06707514971914463 2307 52-53 0.044081150047340145 2307 54-55 0.11475813875363627 2307 56-57 0.10055933175025444 2307 58-59 0.24218701559139788 2307 60-61 -0.11169837223484791 2307 62-63 0.08444651598922803 2307 64-65 0.1538336967328604 2307 66-67 0.14183739357353176 2307 68-69 0.11937431296897927 2307 70-71 0.018880581020443543 2307 72-73 -0.14611601875009228 2307 74-75 0.09094243090201815 2307 76-77 0.25802259177794085 2307 78-79 0.36720953151628777 2307 80-81 0.313446122188374 2307 82-83 0.40750214566141807 2307 84-85 0.30461192423923933 2307 86-87 0.23838234810686032 2307 88-89 0.12302301655694237 2307 90-91 0.2312389582226544 2307 92-93 0.41327356611527577 2307 94-95 0.3723233448373584 2307 96-97 0.650093040031134 2307 98-99 0.35273043153245354 2307 100-101 0.4405873212069231 2201 1 -0.12319870121587684 2201 2 -0.3137759372970592 2201 3 -0.2318829903704014 2201 4 -0.24945434413114498 2201 5 -0.23921892849875093 2201 6 -0.14745676078746328 2201 7 0.12930584623769903 2201 8 -0.3286789382588964 2201 9 -0.17347822352385833 2201 10-11 -0.17384352753420984 2201 12-13 -0.18486513134799765 2201 14-15 -0.03373598930850363 2201 16-17 -0.08125251444731418 2201 18-19 0.002752541269508413 2201 20-21 0.05031404861790634 2201 22-23 0.110472405537962 2201 24-25 0.30883904849105903 2201 26-27 0.06864472441446168 2201 28-29 0.0811547475080232 2201 30-31 0.17911566398928613 2201 32-33 -0.038524652928380476 2201 34-35 -0.042541182971781666 2201 36-37 0.2700004618975598 2201 38-39 0.03410841981698809 2201 40-41 -0.13048744654626176 2201 42-43 0.34859900306984315 2201 44-45 0.27334645286425285 2201 46-47 0.03675326294478509 2201 48-49 0.07077833363539554 2201 50-51 0.10165403221996883 2201 52-53 0.09568290005397984 2201 54-55 0.31657895220349275 2201 56-57 0.23911277838489298 2201 58-59 0.35867280968269455 2201 60-61 0.2776898288189713 2201 62-63 0.36302378372180755 2201 64-65 0.33418832473141435 2201 66-67 0.31934099252877246 2201 68-69 0.18527137894123769 2201 70-71 0.22972121256089295 2201 72-73 0.08319768666545713 2201 74-75 0.2329894822596188 2201 76-77 0.3295205855850831 2201 78-79 0.5044781394044442 2201 80-81 0.6828827469701331 2201 82-83 0.38307004595042926 2201 84-85 0.37052993527134603 2201 86-87 0.35831751220515073 2201 88-89 0.38430982476428 2201 90-91 0.3745505284925521 2201 92-93 0.2870256547597734 2201 94-95 0.21136837515298978 2201 96-97 0.17114704546121473 2201 98-99 0.19885207856443188 2201 100-101 0.006953306429714701 1101 1 0.06435510685325241 1101 2 -0.11553266179706512 1101 3 -0.08194132435189161 1101 4 -0.02926488608736122 1101 5 -0.07828097171685755 1101 6 -0.10511353168644888 1101 7 0.14728665905132488 1101 8 0.004541928946139251 1101 9 0.07847442269328297 1101 10-11 0.01640088514316318 1101 12-13 0.1292614815530655 1101 14-15 0.17118739872913835 1101 16-17 0.19285618254586723 1101 18-19 0.1746609073011598 1101 20-21 0.15951123893151475 1101 22-23 0.005464667904092835 1101 24-25 0.24360435814392645 1101 26-27 0.15089134707808682 1101 28-29 0.18474463413894426 1101 30-31 0.11704441875323113 1101 32-33 0.18039245381341829 1101 34-35 0.17546859568093254 1101 36-37 0.3366662792572086 1101 38-39 0.15367859894924152 1101 40-41 0.014981008850369903 1101 42-43 0.20318029918385605 1101 44-45 0.10102666463672705 1101 46-47 0.10220975652268294 1101 48-49 0.14735918453374097 1101 50-51 -1.2374615381816056E-4 1101 52-53 0.07142628953992158 1101 54-55 0.2524104997570831 1101 56-57 0.3070667709771513 1101 58-59 0.3663527800731785 1101 60-61 0.24241654417327396 1101 62-63 0.2889156231330503 1101 64-65 0.4357072879689312 1101 66-67 0.29265192203718726 1101 68-69 0.038162330603149996 1101 70-71 0.23762888663083004 1101 72-73 0.11467564178450118 1101 74-75 0.26245592077560076 1101 76-77 0.33858541810620224 1101 78-79 0.6444994078399979 1101 80-81 0.35370422353059894 1101 82-83 0.3337256662619197 1101 84-85 0.3984910888627091 1101 86-87 0.47218249384671296 1101 88-89 0.627539998586137 1101 90-91 0.3655259263034836 1101 92-93 0.22845297330086112 1101 94-95 0.24000767804179546 1101 96-97 0.3193632325376221 1101 98-99 0.2816989377824086 1101 100-101 0.31079946027587013 2304 1 0.18473781100379227 2304 2 -2.6265715803930334E-4 2304 3 0.23936671617940775 2304 4 0.05359160139910557 2304 5 0.006474841722329927 2304 6 0.15046898484585824 2304 7 0.15772909450870287 2304 8 0.1667103183609342 2304 9 0.14033700674347216 2304 10-11 0.1744761834139723 2304 12-13 0.15224059976797832 2304 14-15 0.2904795205122994 2304 16-17 0.2389825964970953 2304 18-19 0.2399287004314985 2304 20-21 0.20408599666845362 2304 22-23 0.30662895008437374 2304 24-25 0.38684377607484066 2304 26-27 0.298404891283063 2304 28-29 0.3185751111973687 2304 30-31 0.22005392206894925 2304 32-33 0.25010769378268805 2304 34-35 0.28976167921322116 2304 36-37 0.22940476475434224 2304 38-39 0.18718269846918645 2304 40-41 0.3638909922177689 2304 42-43 0.38843375350899834 2304 44-45 0.2058619483835784 2304 46-47 0.19040189445499323 2304 48-49 0.3220069117713962 2304 50-51 0.2480862175671561 2304 52-53 0.24311753824001414 2304 54-55 0.46486628244086603 2304 56-57 0.5746253785289923 2304 58-59 0.5699771856659268 2304 60-61 0.3708902843076274 2304 62-63 0.4738625520675015 2304 64-65 0.3820458010327741 2304 66-67 0.429654114002318 2304 68-69 0.46491227685834957 2304 70-71 0.5735964011110752 2304 72-73 0.6513828287763062 2304 74-75 0.7278404095622264 2304 76-77 0.7418301127997147 2304 78-79 0.7826256119550443 2304 80-81 0.6497696924167293 2304 82-83 0.4583122941431803 2304 84-85 0.495563462798998 2304 86-87 0.6643929430709861 2304 88-89 0.7263741220623814 2304 90-91 0.6015306764184345 2304 92-93 0.5455802337970432 2304 94-95 0.7064215469785324 2304 96-97 0.693710194593276 2304 98-99 0.6668650632294693 2304 100-101 0.5557129902860432 2202 1 0.09471351416549467 2202 2 -0.03511464288125765 2202 3 0.08148193216883115 2202 4 -0.09605572334878332 2202 5 0.004764463412513464 2202 6 0.01583463515182615 2202 7 0.06687394090797483 2202 8 0.1292699882759223 2202 9 -0.014957655196262465 2202 10-11 -0.01195383018104934 2202 12-13 0.10927348338536547 2202 14-15 0.06987660604720958 2202 16-17 0.17076178992383717 2202 18-19 0.025744871095120914 2202 20-21 0.029244534982751702 2202 22-23 0.20654693103355726 2202 24-25 0.2462873220355064 2202 26-27 0.17575241162338529 2202 28-29 0.2050616114971362 2202 30-31 0.3680023125089562 2202 32-33 0.23288224872211316 2202 34-35 0.28875449593541447 2202 36-37 0.2484173629338784 2202 38-39 0.21979999282270057 2202 40-41 0.2773541160880839 2202 42-43 0.22120933063113313 2202 44-45 0.166447583516792 2202 46-47 0.2137407697962601 2202 48-49 0.34065096864564737 2202 50-51 0.4414072980409287 2202 52-53 0.40324901315631934 2202 54-55 0.6013077844805608 2202 56-57 0.5331010952457262 2202 58-59 0.3287360692113168 2202 60-61 0.3302210790724516 2202 62-63 0.4477054523642465 2202 64-65 0.3815725971077768 2202 66-67 0.2997801357961478 2202 68-69 0.42914410433879624 2202 70-71 0.3338217835073962 2202 72-73 0.4156357221088669 2202 74-75 0.4491940727761303 2202 76-77 0.5063518846245465 2202 78-79 0.4907950071828786 2202 80-81 0.4669223180707611 2202 82-83 0.40054874988911493 2202 84-85 0.3623190593603738 2202 86-87 0.33196601913426704 2202 88-89 0.22895791104475904 2202 90-91 0.2913326707413191 2202 92-93 0.3084787350549938 2202 94-95 0.3225922772658514 2202 96-97 0.2543069626293608 2202 98-99 0.19099035260716946 2202 100-101 0.3906378176371632 1102 1 0.18950441904697968 1102 2 -0.023939306120386306 1102 3 0.07636734217347652 1102 4 0.0971209293715134 1102 5 -0.01770745084044023 1102 6 0.2474534439745426 1102 7 0.14039004556489232 1102 8 0.287352977003664 1102 9 0.17226806759104107 1102 10-11 0.23960198505323405 1102 12-13 0.24849598389621974 1102 14-15 0.08349140581851344 1102 16-17 0.23865190086279142 1102 18-19 0.244730598407763 1102 20-21 0.22951856114605818 1102 22-23 0.3862474369464337 1102 24-25 0.2365673346902355 1102 26-27 0.28764553773456214 1102 28-29 0.18453332620038054 1102 30-31 0.15461447980864307 1102 32-33 0.06932315888254692 1102 34-35 0.1811385143433668 1102 36-37 0.2702675038624065 1102 38-39 0.25349441348270574 1102 40-41 0.3647415975956534 1102 42-43 0.24838420601390254 1102 44-45 0.2330481758275127 1102 46-47 0.20627635754387086 1102 48-49 0.16166500916989435 1102 50-51 0.20638370676886097 1102 52-53 0.2952354061874445 1102 54-55 0.590808116050006 1102 56-57 0.5778755920920844 1102 58-59 0.48416687087972576 1102 60-61 0.41218690724338813 1102 62-63 0.6000352183088324 1102 64-65 0.629433702399453 1102 66-67 0.5172547761229822 1102 68-69 0.5404565463095352 1102 70-71 0.5885789620918587 1102 72-73 0.7199804518866841 1102 74-75 0.6909234925765873 1102 76-77 0.6560086425331484 1102 78-79 0.48255809984599196 1102 80-81 0.4196598066445567 1102 82-83 0.33290452915654 1102 84-85 0.44070939149917265 1102 86-87 0.6242246326156824 1102 88-89 0.5386710440508189 1102 90-91 0.6020738625008555 1102 92-93 0.6454861416107249 1102 94-95 0.6005068040244019 1102 96-97 0.545737896225365 1102 98-99 0.5949639162315599 1102 100-101 0.6407235429414122 2305 1 0.12421168028642171 2305 2 0.061161374531813806 2305 3 -0.033867902564317376 2305 4 -0.040950501815352425 2305 5 0.08519491577871463 2305 6 0.21755641652197255 2305 7 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-0.5543082449027459 1213 98-99 -0.6983150791083084 1213 100-101 -1.1168388593962604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1197.0 3 154.0 4 251.0 5 214.0 6 496.0 7 2891.0 8 14202.0 9 44882.0 10 71666.0 11 58428.0 12 35920.0 13 23307.0 14 16440.0 15 12855.0 16 11348.0 17 10411.0 18 10001.0 19 9985.0 20 10554.0 21 10777.0 22 11642.0 23 12294.0 24 14073.0 25 16077.0 26 19143.0 27 23669.0 28 29470.0 29 35198.0 30 43550.0 31 56601.0 32 77995.0 33 113006.0 34 171652.0 35 290517.0 36 532494.0 37 714686.0 38 133544.0 39 51.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.48457796177866 20.403559984788206 24.387670255897127 11.724191797536006 2 33.79862724520931 24.610519499426754 31.167804562908323 10.42304869245561 3 39.457843654489885 16.398556129626936 30.2857268471353 13.857873368747878 4 31.494806368186957 13.885764355252906 35.089266494253046 19.53016278230709 5 36.40138135504243 12.70195456962664 34.771834214652806 16.124829860678116 6 31.887231504937148 12.980455371569342 40.70176263326658 14.43055049022693 7 39.29320256760895 16.003855355199047 28.61627163046807 16.086670446723932 8 30.560600249522857 32.473672003262294 21.55465930124504 15.41106844596981 9 36.77761929087131 27.637215386737523 19.389323506808285 16.195841815582884 10-11 31.489403221329777 28.38870043224513 24.592483690158964 15.529412656266134 12-13 33.3554744241974 21.629426362553573 24.611935532471126 20.4031636807779 14-15 28.67148729478094 22.50517524583839 26.270578755833757 22.55275870354691 16-17 30.107089030025325 24.853424306079532 27.559508537979692 17.479978125915448 18-19 29.59787919474278 25.020719574626284 27.52480380353409 17.856597427096847 20-21 27.441199797345867 28.491075525799943 26.52777835670538 17.539946320148818 22-23 32.13416633032234 24.015648064456837 25.633454796270083 18.216730808950736 24-25 28.077705554409054 28.02387057714212 25.914767050181496 17.98365681826733 26-27 24.026521433679047 29.750907405618143 27.229192027928406 18.993379132774404 28-29 25.369603515106633 29.00895779968429 25.326059842912212 20.295378842296863 30-31 27.058785371162724 26.33750632093627 26.746294973485263 19.85741333441575 32-33 23.186394469532274 27.21707594336481 29.37010723222162 20.226422354881294 34-35 29.58256910766591 24.250350365642248 26.051092182457715 20.115988344234125 36-37 23.655492619002935 28.367295857187557 29.184631859120124 18.79257966468938 38-39 23.047863005825768 30.86662230372721 24.779251354518472 21.306263335928552 40-41 29.927753566155868 24.98198441502442 23.80133492780196 21.288927091017754 42-43 30.873194098369872 25.495508531189248 24.647725516244524 18.98357185419636 44-45 24.674146487371633 29.313283982408578 25.643792707896022 20.368776822323763 46-47 25.72930791849315 29.59840960765855 25.522307845784947 19.149974628063354 48-49 22.118494320403794 27.555438614875616 28.98731649235671 21.338750572363878 50-51 19.637347759032707 26.197102457345196 29.33767633147013 24.827873452151973 52-53 26.750923547066208 26.796163542107465 25.1433185197504 21.30959439107593 54-55 28.223082291636416 26.47573808969308 25.86302878942225 19.438150829248258 56-57 29.37921166112974 25.840595311698277 24.10128924595712 20.678903781214863 58-59 26.290315508008195 24.672389485255746 24.955879145400356 24.081415861335703 60-61 21.05218906979622 24.69293960367402 26.6027341831347 27.652137143395063 62-63 25.260516896495446 24.72006817051739 26.913177482473422 23.10623745051375 64-65 22.739242218855587 28.31540164192368 28.269564890604855 20.675791248615884 66-67 20.7416932463172 27.978279498667142 26.653507327632433 24.626519927383224 68-69 19.768262825257644 23.960940613790356 34.67437225986449 21.596424301087513 70-71 19.43777695891568 31.315080720111204 29.583263285204737 19.663879035768378 72-73 21.240326932464832 35.07116998511201 25.07864718307334 18.60985589934982 74-75 22.354065809042435 33.983698587041374 24.96132038560834 18.700915218307856 76-77 21.488975847881804 32.7819676124975 24.99007670724275 20.73897983237794 78-79 21.665599733462756 33.78871838617467 24.97604617955063 19.569635700811943 80-81 21.03615033024016 34.265035376910966 24.688734795366468 20.0100794974824 82-83 20.667728934224453 33.1414949498631 25.63506363734383 20.55571247856862 84-85 21.304782631145724 31.794219443372928 25.902286526563255 20.99871139891809 86-87 19.664119645156973 32.11145267541657 27.003356905383104 21.221070774043348 88-89 19.530395618276113 32.167203473275116 27.01458138481108 21.287819523637683 90-91 20.957973825235747 30.968258273059206 25.98188578322646 22.091882118478583 92-93 21.25435467340732 31.20928182305856 24.943333979137797 22.593029524396325 94-95 21.55658879194445 32.34905614197391 24.78437946936612 21.309975596715518 96-97 20.904625181690218 33.396715811170814 23.743365852427605 21.955293154711367 98-99 21.314060626325656 30.95818834175687 25.345460597366877 22.382290434550594 100-101 22.7102901637627 31.612012048028156 24.808978938722966 20.868718849486182 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1044.0 1 638.0 2 187.0 3 184.0 4 194.5 5 180.5 6 246.0 7 332.0 8 441.5 9 579.0 10 708.0 11 850.0 12 936.5 13 887.5 14 786.5 15 746.5 16 716.5 17 667.5 18 624.0 19 544.5 20 541.0 21 626.0 22 695.5 23 786.5 24 900.0 25 1055.5 26 1306.5 27 1632.5 28 2013.5 29 2453.0 30 3164.5 31 4411.0 32 6789.5 33 10819.5 34 17401.0 35 28570.0 36 45073.5 37 65909.0 38 90245.0 39 108280.5 40 117093.0 41 120124.5 42 148701.5 43 185528.0 44 179485.5 45 160797.0 46 161236.0 47 155615.5 48 133802.0 49 117220.0 50 104424.0 51 98167.5 52 97393.5 53 98822.0 54 85712.5 55 57976.5 56 41662.5 57 34555.5 58 29773.0 59 24588.0 60 19199.5 61 15110.5 62 11720.5 63 9069.0 64 7222.5 65 5521.0 66 4156.0 67 3256.5 68 2524.0 69 1884.0 70 1432.5 71 1131.5 72 796.0 73 532.0 74 397.0 75 288.5 76 206.5 77 161.0 78 128.0 79 99.0 80 84.0 81 77.0 82 58.0 83 42.0 84 37.5 85 36.5 86 27.0 87 19.0 88 20.0 89 16.5 90 13.5 91 14.0 92 12.5 93 7.5 94 4.0 95 4.5 96 3.0 97 1.5 98 1.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15952205466223457 2 0.15236741101459284 3 0.12026615274369228 4 0.11451215361966292 5 0.1272693753617543 6 0.14699196446451276 7 0.12238604715780835 8 0.11375504847176433 9 0.10588115493361891 10-11 0.09514918946215628 12-13 0.12335135622137906 14-15 0.11807054781478633 16-17 0.10985595696008654 18-19 0.10720608894244146 20-21 0.10077069518530338 22-23 0.11265724600731136 24-25 0.3956631502918072 26-27 0.28847598897806326 28-29 0.37100045009901045 30-31 0.2104752311158102 32-33 0.24853869242641222 34-35 0.2609552168519492 36-37 0.2574346779142207 38-39 0.27672193155693753 40-41 0.2693969392510186 42-43 0.3232081876379114 44-45 0.271611471808622 46-47 0.2962363167440239 48-49 0.2962930996301163 50-51 0.29614167860053653 52-53 0.299416158365198 54-55 0.2785200562831967 56-57 0.2899523440164655 58-59 0.2908230149365489 60-61 0.27040010357198424 62-63 0.2676745250395493 64-65 0.2760405369238288 66-67 0.2749616620880733 68-69 0.2722171559269409 70-71 0.24787622542200094 72-73 0.23831777292978115 74-75 0.2472705413036821 76-77 0.24329573927721443 78-79 0.240835147546544 80-81 0.2494282909751931 82-83 0.28970628484339844 84-85 0.29440033676036975 86-87 0.2846147527237804 88-89 0.2915044095696576 90-91 0.26803414998480113 92-93 0.25304346805640887 94-95 0.24000233188385553 96-97 0.2391505885924696 98-99 0.25974384861531147 100-101 0.25942207892745456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2641641.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.28226136439254 #Duplication Level Percentage of deduplicated Percentage of total 1 87.10360589262203 36.82937430132887 2 5.6992069802658945 4.819507182187459 3 2.185678684294045 2.7724631216370734 4 1.0579596518423546 1.7893170604872068 5 0.6408816518751508 1.3548962754114382 6 0.4733195275338997 1.2007811983235483 7 0.3711093830710692 1.0983940750852597 8 0.23237226845745762 0.786017999100401 9 0.2041757117794776 0.7769709728748678 >10 1.6137553385157826 13.583234722285509 >50 0.21314794498354583 6.475807078915348 >100 0.18200948385323376 15.339470238424271 >500 0.015566953725815028 4.428238844785802 >1k 0.006485188804181898 4.51930772725048 >5k 1.8133459459195177E-4 0.41479750660015063 >10k+ 5.440037815126543E-4 3.8114216953020814 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 29931 1.1330457090876467 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 23446 0.8875543648815263 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11636 0.4404837750474042 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11566 0.43783390702975916 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 11437 0.4329505788258132 Illumina Single End PCR Primer 1 (97% over 34bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 11405 0.4317392105891754 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5799 0.21952263763319846 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5021 0.190071247379943 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4772 0.18064528828860546 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3513 0.13298551922838872 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3305 0.12511162569024328 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004731907174366236 0.0 0.0 2 0.0051861702631053955 0.0 0.0 3 0.005526867579659764 0.0 0.0 4 0.005829709638819204 0.0 0.0 5 0.006813946331087381 0.0 0.0 6 0.00741963044940626 0.0 0.0 7 0.008479577656464296 0.0 0.0 8 0.009350248576547683 0.0 0.0 9 0.01071303784276516 0.0 0.0 10-11 0.017034865827718453 0.0 0.0 12-13 0.022467095263890895 0.0 0.0 14-15 0.027785758927878542 0.0 0.0 16-17 0.033918310625857186 0.0 0.0 18-19 0.04054298066996992 0.0 0.0 20-21 0.04529381547303362 0.0 0.0 22-23 0.05002572264739985 0.0 0.0 24-25 0.0557797217714292 0.0 0.0 26-27 0.06134444460848389 0.0 0.0 28-29 0.06800696990999155 0.0 0.0 30-31 0.11949011996709621 0.0 0.0 32-33 0.1291053553454084 0.0 0.0 34-35 0.13720638042792338 0.0 0.0 36-37 0.14500456345127896 0.0 0.0 38-39 0.15382483842429762 0.0 0.0 40-41 0.16404575792092868 0.0 0.0 42-43 0.17487236153587865 0.0 0.0 44-45 0.1870428267883486 0.0 0.0 46-47 0.20002718007480957 0.0 0.0 48-49 0.2143175397413956 0.0 0.0 50-51 0.22989497815940924 0.0 0.0 52-53 0.2476301662489339 0.0 0.0 54-55 0.2678827289552214 0.0 0.0 56-57 0.29038767947650723 0.0 0.0 58-59 0.31455826132316994 0.0 0.0 60-61 0.34016734295084006 0.0 0.0 62-63 0.36994050289195235 0.0 0.0 64-65 0.40207961642024787 0.0 0.0 66-67 0.43679288745139855 0.0 0.0 68-69 0.4740424607280096 0.0 0.0 70-71 0.5169892502425576 0.0 0.0 72-73 0.5625101972599608 0.0 0.0 74-75 0.6110027819828661 0.0 0.0 76-77 0.6715711938147537 0.0 0.0 78-79 0.7375150521967216 0.0 0.0 80-81 0.8081908177530557 0.0 0.0 82-83 0.8877625687971984 0.0 0.0 84-85 0.9812650545626753 0.0 0.0 86-87 1.0845531243647415 0.0 0.0 88-89 1.1988760016974296 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 5770 0.0 88.44035 2 GAGCGGC 3390 0.0 88.40102 9 CGGGAGA 4805 0.0 88.23348 4 AGAGCGG 3660 0.0 85.37604 8 ATTCGGA 790 0.0 83.44527 2 TCGGGAG 5250 0.0 83.19354 3 TCGGAGA 1050 0.0 79.94167 4 GTTCGGA 540 0.0 79.921135 2 CGGAGAG 1640 0.0 79.80948 5 AAGCGGC 1045 0.0 79.407524 9 ATCCGGA 840 0.0 78.47828 2 TCGGGAA 1410 0.0 75.33871 3 AAGGGGC 2290 0.0 72.265305 9 AAGCGTC 420 0.0 72.25544 9 GAGAGCG 5240 0.0 72.121864 7 TTGGGAG 2475 0.0 69.937 3 CCGGAGA 900 0.0 69.55378 4 CGGAAAG 1595 0.0 69.276276 5 GATCCGG 1025 0.0 68.019295 1 TCCGGAG 780 0.0 67.48662 3 >>END_MODULE