FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641641
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA299311.1330457090876467No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC234460.8875543648815263No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC116360.4404837750474042No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT115660.43783390702975916No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT114370.4329505788258132Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT114050.4317392105891754No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT57990.21952263763319846No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC50210.190071247379943No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT47720.18064528828860546No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC35130.13298551922838872No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33050.12511162569024328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA57700.088.440352
GAGCGGC33900.088.401029
CGGGAGA48050.088.233484
AGAGCGG36600.085.376048
ATTCGGA7900.083.445272
TCGGGAG52500.083.193543
TCGGAGA10500.079.941674
GTTCGGA5400.079.9211352
CGGAGAG16400.079.809485
AAGCGGC10450.079.4075249
ATCCGGA8400.078.478282
TCGGGAA14100.075.338713
AAGGGGC22900.072.2653059
AAGCGTC4200.072.255449
GAGAGCG52400.072.1218647
TTGGGAG24750.069.9373
CCGGAGA9000.069.553784
CGGAAAG15950.069.2762765
GATCCGG10250.068.0192951
TCCGGAG7800.067.486623