##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_30245-1.3420000003577e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2205418 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.905357170386747 33.0 33.0 33.0 27.0 33.0 2 31.82767575126348 33.0 33.0 33.0 33.0 33.0 3 31.752804230309174 33.0 33.0 33.0 33.0 33.0 4 35.47296702937946 37.0 37.0 37.0 33.0 37.0 5 35.47453725325539 37.0 37.0 37.0 33.0 37.0 6 35.60383972562118 37.0 37.0 37.0 33.0 37.0 7 35.44308516571462 37.0 37.0 37.0 33.0 37.0 8 35.47610112912835 37.0 37.0 37.0 33.0 37.0 9 35.3599403831836 37.0 37.0 37.0 33.0 37.0 10-11 35.30896773310094 37.0 37.0 37.0 33.0 37.0 12-13 35.273483530106304 37.0 37.0 37.0 33.0 37.0 14-15 37.01615317368408 40.0 37.0 40.0 33.0 40.0 16-17 36.990835977578854 40.0 37.0 40.0 33.0 40.0 18-19 37.03767403730268 40.0 37.0 40.0 33.0 40.0 20-21 37.02427362976089 40.0 37.0 40.0 33.0 40.0 22-23 37.1404463915684 40.0 37.0 40.0 33.0 40.0 24-25 36.83185069678402 40.0 37.0 40.0 33.0 40.0 26-27 36.75875321594364 40.0 37.0 40.0 33.0 40.0 28-29 36.627532739825284 40.0 37.0 40.0 33.0 40.0 30-31 36.6810362026609 40.0 37.0 40.0 33.0 40.0 32-33 36.6321835588537 40.0 37.0 40.0 33.0 40.0 34-35 36.5675753984052 40.0 37.0 40.0 33.0 40.0 36-37 36.47431235257896 40.0 37.0 40.0 33.0 40.0 38-39 36.46827789561888 40.0 37.0 40.0 33.0 40.0 40-41 36.310945816167276 40.0 37.0 40.0 33.0 40.0 42-43 36.20042663114204 40.0 37.0 40.0 33.0 40.0 44-45 36.11103291983651 40.0 37.0 40.0 33.0 40.0 46-47 36.00370111244217 40.0 37.0 40.0 27.0 40.0 48-49 35.89454198705189 37.0 37.0 40.0 27.0 40.0 50-51 34.820490492051846 37.0 35.0 38.5 27.0 38.5 52-53 35.233096401679866 37.0 37.0 38.5 27.0 40.0 54-55 35.95964143758689 37.0 37.0 40.0 27.0 40.0 56-57 36.05905864557195 37.0 37.0 40.0 27.0 40.0 58-59 35.857217996769776 37.0 37.0 40.0 27.0 40.0 60-61 35.6916917790641 37.0 37.0 40.0 27.0 40.0 62-63 35.49529227565931 37.0 37.0 40.0 27.0 40.0 64-65 35.26779685302287 37.0 37.0 40.0 27.0 40.0 66-67 35.054267943763946 37.0 37.0 40.0 27.0 40.0 68-69 34.87074309722692 37.0 33.0 40.0 27.0 40.0 70-71 34.586717347913186 37.0 33.0 37.0 27.0 40.0 72-73 34.398148559592784 37.0 33.0 37.0 27.0 40.0 74-75 34.20343354411726 37.0 33.0 37.0 27.0 40.0 76-77 33.94364174954589 37.0 33.0 37.0 27.0 37.0 78-79 33.67576259919888 37.0 33.0 37.0 27.0 37.0 80-81 33.38685931646518 37.0 33.0 37.0 27.0 37.0 82-83 33.140034904947726 37.0 33.0 37.0 27.0 37.0 84-85 32.935890384498535 37.0 33.0 37.0 27.0 37.0 86-87 32.77290064740562 37.0 33.0 37.0 24.5 37.0 88-89 32.606545335170026 37.0 33.0 37.0 22.0 37.0 90-91 32.455066794594046 37.0 33.0 37.0 22.0 37.0 92-93 32.30757933416704 33.0 33.0 37.0 22.0 37.0 94-95 32.160393630595195 33.0 33.0 37.0 22.0 37.0 96-97 32.03356075809665 33.0 33.0 37.0 22.0 37.0 98-99 31.879934778803836 33.0 33.0 37.0 22.0 37.0 100-101 30.63461053641532 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19423497284724434 2306 2 0.17788871186001742 2306 3 0.22116399954762045 2306 4 0.24496539318202082 2306 5 0.258910782681113 2306 6 0.22478520638811972 2306 7 0.2063232203559764 2306 8 0.22350270027553165 2306 9 0.28085854408040944 2306 10-11 0.18000919480348188 2306 12-13 0.2272999927265218 2306 14-15 0.38102286004978225 2306 16-17 0.46142297382985475 2306 18-19 0.3841038676801318 2306 20-21 0.42353889102512454 2306 22-23 0.3098186633030693 2306 24-25 0.5309227383910908 2306 26-27 0.4270160007383481 2306 28-29 0.3859929844101231 2306 30-31 0.4267605467568103 2306 32-33 0.3472260237005784 2306 34-35 0.3848520489509326 2306 36-37 0.3231857896536994 2306 38-39 0.331957214699095 2306 40-41 0.27040308501435817 2306 42-43 0.2480815468262918 2306 44-45 0.34309047865181697 2306 46-47 0.2592772498577176 2306 48-49 0.36384109959509914 2306 50-51 0.3118806377148502 2306 52-53 0.4440401804764562 2306 54-55 0.7051777447369645 2306 56-57 0.3825008440824007 2306 58-59 0.6741932665827477 2306 60-61 0.41328508369300465 2306 62-63 0.4037988280500713 2306 64-65 0.4857034324664866 2306 66-67 0.32403120775270366 2306 68-69 0.3560449461087245 2306 70-71 0.5137233582235723 2306 72-73 0.5394425162272682 2306 74-75 0.33917921145874885 2306 76-77 0.3701194068911562 2306 78-79 0.42262451246389077 2306 80-81 0.21464351616049981 2306 82-83 0.19242532434623882 2306 84-85 0.1347621117707405 2306 86-87 0.24909370194634306 2306 88-89 0.3328251295458102 2306 90-91 0.34831056982370256 2306 92-93 0.2003681800463113 2306 94-95 0.15771627334382998 2306 96-97 0.2279417017860439 2306 98-99 0.3386954064942529 2306 100-101 0.2567584219809085 2307 1 0.22326817117812325 2307 2 0.05734178670612877 2307 3 0.09633924311848219 2307 4 0.17862419571969212 2307 5 0.1542897808307302 2307 6 0.12097433727441853 2307 7 0.3717743928947783 2307 8 0.19245567599396907 2307 9 0.216086278116137 2307 10-11 0.24118129697107804 2307 12-13 0.2599430906080258 2307 14-15 0.47818420922466487 2307 16-17 0.41040496799547554 2307 18-19 0.3836970311747123 2307 20-21 0.41293488485088403 2307 22-23 0.3690082119417184 2307 24-25 0.497730602992128 2307 26-27 0.4682829114479148 2307 28-29 0.43638878706107675 2307 30-31 0.4665316916342661 2307 32-33 0.4344144205700289 2307 34-35 0.35106013210489095 2307 36-37 0.3780821658290776 2307 38-39 0.3293368217758683 2307 40-41 0.3964996849350584 2307 42-43 0.5271079596316994 2307 44-45 0.3513080450201542 2307 46-47 0.19724604116090916 2307 48-49 0.2305432870124946 2307 50-51 0.3779769662145327 2307 52-53 0.3681007097238407 2307 54-55 0.38408402423925025 2307 56-57 0.453815536692197 2307 58-59 0.30804395197982615 2307 60-61 0.23349816099371168 2307 62-63 0.09456886128144504 2307 64-65 0.3084578365675341 2307 66-67 0.27666735489594174 2307 68-69 0.2029233683737388 2307 70-71 0.2927990398443612 2307 72-73 0.160692185801679 2307 74-75 0.19494254177104864 2307 76-77 0.3906568323093822 2307 78-79 0.2797667795610508 2307 80-81 0.007156308351120799 2307 82-83 0.1153154979612836 2307 84-85 0.3260608259431592 2307 86-87 0.3872974494445458 2307 88-89 0.1778419576489796 2307 90-91 0.32115076656853603 2307 92-93 0.2437011357503991 2307 94-95 0.25990304168716705 2307 96-97 0.23827900730697849 2307 98-99 0.2890793492267818 2307 100-101 0.18915836203226277 2201 1 0.06796527660892693 2201 2 0.06663815157314801 2201 3 0.04337549491016901 2201 4 0.10000797498390313 2201 5 0.002861408478445071 2201 6 0.11757560738112005 2201 7 0.1607555204628639 2201 8 0.06373991711517135 2201 9 0.1272330570618294 2201 10-11 0.02404257066115889 2201 12-13 0.004312715548302037 2201 14-15 0.16163176257710887 2201 16-17 0.1361462783155929 2201 18-19 0.18720699494281945 2201 20-21 0.2589386772541289 2201 22-23 0.3006989170699157 2201 24-25 0.42661283941512806 2201 26-27 0.34511068059905625 2201 28-29 0.25960605891965827 2201 30-31 0.321033552997541 2201 32-33 0.13965784109877433 2201 34-35 0.08883784352390478 2201 36-37 0.1273095009939098 2201 38-39 0.20401182973891707 2201 40-41 0.22817297088823096 2201 42-43 0.15655308425345282 2201 44-45 0.04794514885834644 2201 46-47 0.1542398399346041 2201 48-49 0.1493943182276567 2201 50-51 0.18765555755072683 2201 52-53 0.2601729943233053 2201 54-55 0.21944868222619363 2201 56-57 0.2779530386829592 2201 58-59 0.39858384686754533 2201 60-61 0.25233276841526475 2201 62-63 0.2527041137101733 2201 64-65 0.318958612965055 2201 66-67 0.25927928553776525 2201 68-69 0.43622285873844646 2201 70-71 0.3800785628575767 2201 72-73 0.25900600965898946 2201 74-75 0.34452348618895456 2201 76-77 0.22024180802207383 2201 78-79 0.18436295331483166 2201 80-81 0.37202033924776146 2201 82-83 0.29250807440657667 2201 84-85 0.39579028127044324 2201 86-87 0.33459175387200446 2201 88-89 0.4660113516607609 2201 90-91 0.5239268853772643 2201 92-93 0.4791048624584775 2201 94-95 0.4434625754505035 2201 96-97 0.5257419293487047 2201 98-99 0.40381970398071587 2201 100-101 0.39430970977872093 1101 1 0.2293605214845833 1101 2 0.1022866829814717 1101 3 0.19528284283559572 1101 4 0.2909385521553389 1101 5 0.26763549040168755 1101 6 0.13477236886328825 1101 7 0.24589037592165397 1101 8 0.23693646335841834 1101 9 0.18042356005861393 1101 10-11 0.23342036462783966 1101 12-13 0.15330854322471765 1101 14-15 0.3735644838592691 1101 16-17 0.3010410182076413 1101 18-19 0.4094035080723941 1101 20-21 0.21063865076319388 1101 22-23 0.3071012399945161 1101 24-25 0.4034465094041977 1101 26-27 0.28095603143848535 1101 28-29 0.3776900600455235 1101 30-31 0.29438780313827095 1101 32-33 0.3462816117534331 1101 34-35 0.3608727205065847 1101 36-37 0.39044735324067403 1101 38-39 0.4105706147980257 1101 40-41 0.4168431255291196 1101 42-43 0.3230068575572034 1101 44-45 0.25009546840535535 1101 46-47 0.37621159396618964 1101 48-49 0.26277307249103643 1101 50-51 0.3176354409905784 1101 52-53 0.3941718436229493 1101 54-55 0.47231260156127064 1101 56-57 0.5479559775220011 1101 58-59 0.6575400623051451 1101 60-61 0.5447458036151076 1101 62-63 0.5586188377076624 1101 64-65 0.6069240671191594 1101 66-67 0.34221353004917177 1101 68-69 0.2623145753515814 1101 70-71 0.3161739219754338 1101 72-73 0.39883162474558276 1101 74-75 0.4208236120209321 1101 76-77 0.3435203106214999 1101 78-79 0.35282341558179553 1101 80-81 0.42513426686125655 1101 82-83 0.23818280701361516 1101 84-85 0.2468971059997358 1101 86-87 0.2509857155925914 1101 88-89 0.34181973935571364 1101 90-91 0.4316676218254898 1101 92-93 0.1803613196259306 1101 94-95 0.22819261618033693 1101 96-97 0.28276347807548774 1101 98-99 0.1395810288590269 1101 100-101 0.1939725711317415 2304 1 0.15416737102199463 2304 2 0.11443243854063567 2304 3 0.14851283796625836 2304 4 0.30577583506395456 2304 5 0.06948786826242781 2304 6 0.12914422408159965 2304 7 0.24128844441703023 2304 8 0.20742542618913262 2304 9 0.24305300333081448 2304 10-11 0.22785308658855996 2304 12-13 0.186054039085775 2304 14-15 0.30167781773364766 2304 16-17 0.36757343989243907 2304 18-19 0.22448425795066385 2304 20-21 0.1530907689258285 2304 22-23 0.1792865589362478 2304 24-25 0.3304518656775386 2304 26-27 0.2937208017679822 2304 28-29 0.3360521360072113 2304 30-31 0.2607122634531578 2304 32-33 0.2733910501562917 2304 34-35 0.14651973397842255 2304 36-37 0.16221492344030963 2304 38-39 0.19795557665486996 2304 40-41 0.3559617750989972 2304 42-43 0.1699858382421553 2304 44-45 0.2663748873308478 2304 46-47 0.16210430618824034 2304 48-49 0.20002757662998505 2304 50-51 0.07923623631601373 2304 52-53 0.21847005008293507 2304 54-55 0.44213852267797193 2304 56-57 0.37094717213326334 2304 58-59 0.4436411416753785 2304 60-61 0.3902175208688021 2304 62-63 0.4639667925851185 2304 64-65 0.5030641012045365 2304 66-67 0.3809899056375414 2304 68-69 0.3467738757898218 2304 70-71 0.4820940242315572 2304 72-73 0.48830147541917057 2304 74-75 0.519432198289401 2304 76-77 0.43384452746941093 2304 78-79 0.39947452741429146 2304 80-81 0.44024666744558516 2304 82-83 0.4380401759472292 2304 84-85 0.43600520533426135 2304 86-87 0.528539886993336 2304 88-89 0.41387581791438777 2304 90-91 0.4295807041173205 2304 92-93 0.3634669437129361 2304 94-95 0.4520398000790493 2304 96-97 0.6342687126153308 2304 98-99 0.507243137282984 2304 100-101 0.3542888753031441 2202 1 -0.00926652169310671 2202 2 -0.02540759532209691 2202 3 -0.11875936020127398 2202 4 -0.09580089841693962 2202 5 -0.18375598750349553 2202 6 -0.04671094218971206 2202 7 -0.009733522266984096 2202 8 -0.125576452134311 2202 9 -0.21794083259366914 2202 10-11 -0.13562719101529552 2202 12-13 -0.10838929815705711 2202 14-15 0.08048715467664636 2202 16-17 -0.15074241827210244 2202 18-19 -0.14851814947797237 2202 20-21 -0.07378812771347754 2202 22-23 -0.18343233629209976 2202 24-25 -0.03958684718589467 2202 26-27 -0.07001432817144604 2202 28-29 0.0255952536713977 2202 30-31 -0.02042866371551355 2202 32-33 -0.014272509487092577 2202 34-35 -0.04103094989004319 2202 36-37 -0.03837097237887832 2202 38-39 -0.11974334485237392 2202 40-41 -0.16744178225160056 2202 42-43 -0.04784853419499058 2202 44-45 0.04701898472148258 2202 46-47 -0.13309088953658943 2202 48-49 -0.1241304847285889 2202 50-51 -0.08908041550631651 2202 52-53 -0.04856475622047185 2202 54-55 0.07641713183678434 2202 56-57 0.04558874745578834 2202 58-59 -0.05432444885011023 2202 60-61 -0.09782276167337045 2202 62-63 0.07638912435171363 2202 64-65 0.016304076865701234 2202 66-67 0.11035630216661474 2202 68-69 0.12261105248450122 2202 70-71 0.04932336487830469 2202 72-73 0.14665013810579097 2202 74-75 0.07328135039832517 2202 76-77 0.18441375647496017 2202 78-79 0.010746937223899522 2202 80-81 0.100448432893252 2202 82-83 0.14463053388689673 2202 84-85 8.163588717735593E-4 2202 86-87 -0.11323667188284503 2202 88-89 0.07426964854781914 2202 90-91 -0.07470257880404318 2202 92-93 0.03252769380772946 2202 94-95 0.13371212523182407 2202 96-97 0.17118244201329702 2202 98-99 0.03538412618058828 2202 100-101 0.09207615737794228 1102 1 0.16326539982666688 1102 2 0.08244962842581316 1102 3 0.17621742414797836 1102 4 0.18204018762252616 1102 5 0.12742574574301102 1102 6 0.18057947993877121 1102 7 0.15535594292369836 1102 8 0.21168042054766545 1102 9 0.24082661453410026 1102 10-11 0.197416536512236 1102 12-13 0.2553974717531915 1102 14-15 0.1810275555713048 1102 16-17 0.2986396773650597 1102 18-19 0.2326237349533855 1102 20-21 0.2905330268404711 1102 22-23 0.2045450709160761 1102 24-25 0.17980989979182738 1102 26-27 0.28609818279937116 1102 28-29 0.23394156323227833 1102 30-31 0.36838217119856154 1102 32-33 0.3814198548287564 1102 34-35 0.4398939956098076 1102 36-37 0.2922892580789309 1102 38-39 0.4018709214481291 1102 40-41 0.2994918408055085 1102 42-43 0.32295424602074974 1102 44-45 0.29917952660873937 1102 46-47 0.3562267260462022 1102 48-49 0.33452017635122644 1102 50-51 0.25419093864560693 1102 52-53 0.3522166486095202 1102 54-55 0.4658313613319862 1102 56-57 0.5052391678855699 1102 58-59 0.6647891804355837 1102 60-61 0.6502732718477588 1102 62-63 0.6221197095559816 1102 64-65 0.5904579088636623 1102 66-67 0.6071163740785082 1102 68-69 0.5812369407662601 1102 70-71 0.5562491314194489 1102 72-73 0.5730572330351507 1102 74-75 0.5351259329412912 1102 76-77 0.590255890049896 1102 78-79 0.5304023829927118 1102 80-81 0.43116705637503117 1102 82-83 0.43063956534127357 1102 84-85 0.45060897516236054 1102 86-87 0.43638501210119074 1102 88-89 0.5870771851407852 1102 90-91 0.5527092300348926 1102 92-93 0.43991281880418853 1102 94-95 0.43363816073478745 1102 96-97 0.42573532839816863 1102 98-99 0.4069265513667375 1102 100-101 0.5460677629383746 2305 1 0.30026478817024227 2305 2 0.34534174388324246 2305 3 0.30967964222776345 2305 4 0.3394905026741313 2305 5 0.31392707099859507 2305 6 0.33658220812059625 2305 7 0.2878458214662061 2305 8 0.3401753573917077 2305 9 0.2960419564306278 2305 10-11 0.3406454756281505 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6363.0 9 11109.0 10 13854.0 11 12957.0 12 10204.0 13 7508.0 14 6012.0 15 5078.0 16 4736.0 17 4824.0 18 5016.0 19 5493.0 20 6209.0 21 6896.0 22 7753.0 23 8656.0 24 9804.0 25 11333.0 26 13454.0 27 16070.0 28 19680.0 29 24191.0 30 29944.0 31 39154.0 32 53619.0 33 76696.0 34 116585.0 35 197693.0 36 389186.0 37 748698.0 38 332042.0 39 1121.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.41143151998445 24.88633070519931 26.18263760003125 14.519600174784994 2 29.50835307887361 23.68915273805582 34.61675938781785 12.185734795252714 3 37.93308228824541 20.05388358639126 26.708515586195126 15.30451853916821 4 28.32662782282924 19.519845496321274 36.23617924823827 15.917347432611209 5 31.044363945451593 18.40536620937225 35.08990752705944 15.460362318116724 6 23.816003106429264 18.880232164713416 41.76453357010016 15.539231158757156 7 31.777444108807966 19.01387954953781 32.160790102290676 17.047886239363542 8 27.834252142058435 28.21165757391821 27.823901072833845 16.130189211189506 9 30.408308902771964 26.806234854792127 25.638879229649696 17.14657701278621 10-11 24.31877627674483 29.196693314572 29.862297091047324 16.622233317635846 12-13 24.97905171405902 26.115037929394457 29.915151212457957 18.990759144088564 14-15 23.18089146902314 26.921183143852673 31.03912705037399 18.858798336750198 16-17 22.430591526303196 28.08298373928009 32.0561668754168 17.430257858999916 18-19 21.866644936929667 28.290710287751807 32.2603746064417 17.582270168876825 20-21 21.64267740129267 29.15255783740501 31.626315358236596 17.578449403065733 22-23 22.24620333878281 28.800985536117942 31.240627804848593 17.71218332025066 24-25 21.60747109438664 29.37342197698662 31.17163676320883 17.84747016541791 26-27 21.16842536159374 29.895888292549806 31.042618029655234 17.89306831620122 28-29 21.207641358972662 29.533070803592747 30.926909343932852 18.332378493501743 30-31 21.499249816118034 29.158169392270388 30.869606611888738 18.472974179722833 32-33 20.361957624462146 29.240685044447968 32.36523822913723 18.032119101952656 34-35 20.13691682071249 30.37564597507879 31.660650714104975 17.826786490103743 36-37 20.43966256677769 30.337390028092155 31.32276378424079 17.900183620889358 38-39 20.654008040661253 29.34159212914718 31.80333691307459 18.201062917116975 40-41 21.055391069746886 29.645824740838222 30.649276593383956 18.649507596030936 42-43 20.942196965733125 29.17482409305267 31.370356636691017 18.51262230452319 44-45 20.34041510188147 29.593431346847197 31.669963923065886 18.396189628205445 46-47 20.875169861097685 29.744108165272714 30.885347312641077 18.49537466098852 48-49 20.438306142935964 30.018344250580736 30.65698161799656 18.886367988486743 50-51 20.33671762742502 29.521389463399633 30.80868269070257 19.33321021847278 52-53 21.27504191658168 29.178967200900285 30.282444525917317 19.263546356600713 54-55 21.57981131890988 29.276698233272114 30.059454849036708 19.084035598781295 56-57 21.748217127177238 29.305996897611397 29.926770257003398 19.019015718207967 58-59 21.408350961807425 29.256702269286066 30.422410228957155 18.91253653994936 60-61 20.33466926076232 29.518531815225025 30.321748045163094 19.825050878849567 62-63 20.91113365023001 29.481895077597397 29.984341602704152 19.622629669468445 64-65 20.25473002978005 29.542662414041576 30.45875644141048 19.7438511147679 66-67 20.083557092837097 29.133839826773876 30.457933705946242 20.32466937444278 68-69 19.896986234590624 29.291575158371213 31.353351130602565 19.458087476435594 70-71 19.505727190687377 30.836994992259935 30.363635123738685 19.293642693314 72-73 19.5335495646205 31.20847075081655 29.780730564754602 19.477249119808345 74-75 19.853605733385223 30.73502187662529 29.926809686089523 19.484562703899968 76-77 19.922789278238408 30.57165438200623 30.078631373583924 19.426924966171438 78-79 19.874718122594764 30.685331737401295 30.11672412370534 19.3232260162986 80-81 19.46333577168982 31.173152558523963 29.994307975789802 19.369203693996415 82-83 19.319725384970674 30.805090325093925 29.986812311147588 19.88837197878781 84-85 19.369535429802767 30.413308847358756 30.418516854954714 19.798638867883763 86-87 19.60307082871898 30.435041648569307 30.605865592119173 19.35602193059254 88-89 19.86782106243921 30.95022970189581 29.875278044928834 19.30667119073615 90-91 20.055440131070075 30.557688975953663 29.659924451908005 19.72694644106826 92-93 19.628406863363562 30.789272260591215 29.978760845483386 19.60356003056183 94-95 19.285286157610173 31.09396845268983 29.990333104446343 19.630412285253655 96-97 19.587416100136604 31.42547373732552 29.631533847472152 19.355576315065722 98-99 20.07865074282394 30.760952075016927 29.24904636315337 19.91135081900576 100-101 20.054439281763408 30.695470577189155 28.925887008367546 20.32420313267989 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 925.0 1 540.5 2 149.5 3 163.5 4 203.0 5 230.5 6 297.5 7 435.0 8 562.5 9 737.5 10 905.5 11 986.5 12 1008.5 13 959.0 14 860.5 15 835.0 16 864.5 17 889.5 18 987.0 19 1145.5 20 1436.5 21 1899.0 22 2689.0 23 3867.0 24 5368.0 25 7595.5 26 11331.5 27 16366.5 28 22070.0 29 27778.0 30 33246.5 31 38894.0 32 45669.5 33 55028.5 34 67237.0 35 83262.0 36 102735.0 37 122125.5 38 141913.5 39 153473.0 40 154075.5 41 143831.0 42 127210.5 43 116743.0 44 105868.0 45 96201.0 46 92798.5 47 83927.0 48 67444.5 49 54311.0 50 44860.5 51 36359.0 52 29057.0 53 22992.5 54 18544.0 55 14691.5 56 10895.5 57 7706.0 58 5604.5 59 4085.0 60 2925.0 61 2097.0 62 1480.5 63 1066.5 64 810.0 65 590.0 66 419.5 67 308.5 68 228.0 69 171.5 70 142.5 71 119.5 72 87.5 73 80.5 74 75.0 75 60.5 76 55.0 77 49.5 78 35.5 79 20.5 80 21.5 81 25.0 82 18.0 83 15.5 84 15.0 85 12.0 86 9.5 87 6.5 88 12.0 89 11.5 90 4.5 91 5.0 92 4.0 93 3.5 94 4.5 95 4.5 96 3.5 97 2.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1748421387691585 2 0.16572822022854625 3 0.13113160407686886 4 0.12478360111325836 5 0.13802372158021745 6 0.16019638907454278 7 0.13462300570685468 8 0.12455688672170083 9 0.11680325453043369 10-11 0.10453800594717193 12-13 0.1346910200243219 14-15 0.12979398916667953 16-17 0.11902505556769738 18-19 0.11786881217075403 20-21 0.11124875193727447 22-23 0.1251010012614389 24-25 0.42778738542988226 26-27 0.30914774432783265 28-29 0.3951405130455995 30-31 0.22478731922927989 32-33 0.2640542518470422 34-35 0.27779314397542776 36-37 0.27430174234544197 38-39 0.29334575123627354 40-41 0.2860682192672772 42-43 0.3411144735374428 44-45 0.2887207776485002 46-47 0.3142488181378768 48-49 0.3132739462541795 50-51 0.3141808038204096 52-53 0.31758151969377235 54-55 0.2957489237867833 56-57 0.3078554722959548 58-59 0.30819554388329107 60-61 0.286635005246171 62-63 0.2846852614787764 64-65 0.29302835108809305 66-67 0.29212149352186295 68-69 0.28980900672797627 70-71 0.26403158040788643 72-73 0.25450957596247065 74-75 0.2634421229898369 76-77 0.2595199640158918 78-79 0.256572676925644 80-81 0.2652104952439855 82-83 0.3072660148779052 84-85 0.3119816742223016 86-87 0.30211959818954953 88-89 0.30867164410556186 90-91 0.28516136170104717 92-93 0.2694500543661111 94-95 0.25584719087265995 96-97 0.25496300474558564 98-99 0.2754353143032296 100-101 0.27600210028212335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2205418.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.95369617422988 #Duplication Level Percentage of deduplicated Percentage of total 1 92.92955394118626 64.07836228067264 2 3.5493953505617717 4.894878572097211 3 1.073478704891877 2.2206097339986077 4 0.5823513399537809 1.6062110944731465 5 0.38033970164870856 1.3112914115241143 6 0.22229290056321782 0.9196750276274649 7 0.1760285138817728 0.8496471664943477 8 0.13475983502619224 0.743375097670832 9 0.11614566957513302 0.7207805890652595 >10 0.7150818119588764 9.2737638330947 >50 0.05644721631028316 2.674576054192299 >100 0.06050185939919675 8.345532338810633 >500 0.002826399574827889 1.2764015262141346 >1k 7.967554679472312E-4 1.0848952740646787 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2600 0.11789148360990978 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2564 0.11625913999069565 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2563 0.11621379711238414 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2322 0.10528616343931173 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2231 0.10115996151296489 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.708289312955639E-4 0.0 0.0 2 9.068575662300753E-4 0.0 0.0 3 0.0010882290794760902 0.0 0.0 4 0.001133571957787594 0.0 0.0 5 0.0012696005927221052 0.0 0.0 6 0.0013602863493451128 0.0 0.0 7 0.001677686497525639 0.0 0.0 8 0.0020404295240176694 0.0 0.0 9 0.002448515428821203 0.0 0.0 10-11 0.003604758825764549 0.0 0.0 12-13 0.004534287831150376 0.0 0.0 14-15 0.005713202667249473 0.0 0.0 16-17 0.006710745990102556 0.0 2.267143915575188E-5 18-19 0.008093703778603422 0.0 4.534287831150376E-5 20-21 0.009612690202038797 0.0 4.534287831150376E-5 22-23 0.011517091091121954 0.0 9.068575662300752E-5 24-25 0.013421491980205112 0.0 9.068575662300752E-5 26-27 0.015756650213247556 0.0 9.068575662300752E-5 28-29 0.018613251546872295 0.0 9.068575662300752E-5 30-31 0.027885870161574813 0.0 9.068575662300752E-5 32-33 0.031535971865650866 0.0 9.068575662300752E-5 34-35 0.03509538781310391 0.0 9.068575662300752E-5 36-37 0.03888151815211448 0.0 9.068575662300752E-5 38-39 0.042486276977879024 0.0 9.068575662300752E-5 40-41 0.04652179314760286 0.0 9.068575662300752E-5 42-43 0.05114676673537624 0.0 9.068575662300752E-5 44-45 0.056701269328535454 0.0 9.068575662300752E-5 46-47 0.06266385782649819 0.0 9.068575662300752E-5 48-49 0.06910254654673173 0.0 9.068575662300752E-5 50-51 0.0753371923145635 0.0 9.068575662300752E-5 52-53 0.08204793830466606 0.0 9.068575662300752E-5 54-55 0.08948417034775266 0.0 9.068575662300752E-5 56-57 0.09744184549142158 0.0 1.3602863493451128E-4 58-59 0.10762132167235416 0.0 1.3602863493451128E-4 60-61 0.11814086944062305 0.0 1.3602863493451128E-4 62-63 0.1287057600872034 0.0 1.3602863493451128E-4 64-65 0.1400868225433909 0.0 1.3602863493451128E-4 66-67 0.15364434315853048 0.0 1.3602863493451128E-4 68-69 0.16876619307541701 0.0 1.3602863493451128E-4 70-71 0.18529367221996013 0.0 1.5870007409026318E-4 72-73 0.20383890944936517 0.0 1.8137151324601505E-4 74-75 0.22369909014980383 0.0 1.8137151324601505E-4 76-77 0.24562237181341587 0.0 1.8137151324601505E-4 78-79 0.2722386413822686 0.0 1.8137151324601505E-4 80-81 0.30164349796727874 0.0 1.8137151324601505E-4 82-83 0.334879827769611 0.0 1.8137151324601505E-4 84-85 0.37262777396393787 0.0 1.8137151324601505E-4 86-87 0.41570350835986647 0.0 2.0404295240176692E-4 88-89 0.461159743867149 0.0 2.2671439155751881E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 890 0.0 74.61669 2 CGGGAGA 845 0.0 68.48277 4 AGAGCGG 640 0.0 63.731953 8 GAGCGGC 575 0.0 63.514297 9 GATCGGG 1225 0.0 60.031925 1 GAGAGCG 855 0.0 53.252975 7 TCGGGAG 1160 0.0 51.930668 3 TTGTGTT 28885 0.0 49.752346 1 AAGCGGC 215 0.0 46.326496 9 AAGCGTC 155 0.0 42.839558 9 GGTCCGG 105 1.0404619E-9 40.66679 1 GAGCGTC 2075 0.0 40.457947 9 AGAGCGT 2065 0.0 40.423264 8 CGGAAAG 400 0.0 40.31783 5 AGCGGCG 550 0.0 40.099495 10-11 GCGTCGG 270 0.0 39.524593 10-11 GATCCGG 205 0.0 39.344296 1 ATTCGGA 220 0.0 38.810368 2 AAGGGGC 660 0.0 38.805958 9 GGCGCCG 380 0.0 38.75232 56-57 >>END_MODULE