FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205418
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG26000.11789148360990978No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC25640.11625913999069565No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC25630.11621379711238414No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23220.10528616343931173No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA22310.10115996151296489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8900.074.616692
CGGGAGA8450.068.482774
AGAGCGG6400.063.7319538
GAGCGGC5750.063.5142979
GATCGGG12250.060.0319251
GAGAGCG8550.053.2529757
TCGGGAG11600.051.9306683
TTGTGTT288850.049.7523461
AAGCGGC2150.046.3264969
AAGCGTC1550.042.8395589
GGTCCGG1051.0404619E-940.666791
GAGCGTC20750.040.4579479
AGAGCGT20650.040.4232648
CGGAAAG4000.040.317835
AGCGGCG5500.040.09949510-11
GCGTCGG2700.039.52459310-11
GATCCGG2050.039.3442961
ATTCGGA2200.038.8103682
AAGGGGC6600.038.8059589
GGCGCCG3800.038.7523256-57
Produced by FastQC (version 0.11.2)