##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2221032 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80624547507645 33.0 33.0 33.0 27.0 33.0 2 31.644562527689832 33.0 33.0 33.0 27.0 33.0 3 31.565560964452562 33.0 33.0 33.0 27.0 33.0 4 35.236680966325565 37.0 37.0 37.0 33.0 37.0 5 35.24987933537203 37.0 37.0 37.0 33.0 37.0 6 35.47141013726952 37.0 37.0 37.0 33.0 37.0 7 35.22798996142334 37.0 37.0 37.0 33.0 37.0 8 35.27006589729459 37.0 37.0 37.0 33.0 37.0 9 35.066722586617395 37.0 37.0 37.0 33.0 37.0 10-11 35.016992100969276 37.0 37.0 37.0 33.0 37.0 12-13 34.96069529840182 37.0 37.0 37.0 33.0 37.0 14-15 36.58398820908479 40.0 37.0 40.0 33.0 40.0 16-17 36.48484825972791 40.0 37.0 40.0 33.0 40.0 18-19 36.587483431125705 40.0 37.0 40.0 33.0 40.0 20-21 36.54514973219656 40.0 37.0 40.0 33.0 40.0 22-23 36.74831047008777 40.0 37.0 40.0 33.0 40.0 24-25 36.35258902168001 40.0 37.0 40.0 33.0 40.0 26-27 36.207788541542854 40.0 37.0 40.0 30.0 40.0 28-29 36.06293988560273 40.0 37.0 40.0 30.0 40.0 30-31 36.127756151194575 40.0 37.0 40.0 33.0 40.0 32-33 36.119399675466184 40.0 37.0 40.0 33.0 40.0 34-35 36.02143282942344 38.5 37.0 40.0 27.0 40.0 36-37 35.843797162760374 37.0 37.0 40.0 27.0 40.0 38-39 35.82787528500265 37.0 37.0 40.0 27.0 40.0 40-41 35.62941551494981 37.0 37.0 40.0 27.0 40.0 42-43 35.47732022771396 37.0 37.0 40.0 27.0 40.0 44-45 35.33134822010669 37.0 37.0 40.0 27.0 40.0 46-47 35.17665098926985 37.0 37.0 40.0 27.0 40.0 48-49 35.03461746611485 37.0 35.0 40.0 27.0 40.0 50-51 33.8928793011537 37.0 33.0 38.5 24.5 38.5 52-53 34.3509073709879 37.0 33.0 38.5 27.0 40.0 54-55 35.03853411387139 37.0 37.0 40.0 27.0 40.0 56-57 35.100952620223396 37.0 35.0 40.0 27.0 40.0 58-59 34.81491982105616 37.0 33.0 40.0 27.0 40.0 60-61 34.64240722330881 37.0 33.0 40.0 27.0 40.0 62-63 34.40617537252953 37.0 33.0 40.0 27.0 40.0 64-65 34.13181507515425 37.0 33.0 37.0 27.0 40.0 66-67 33.84692701410876 37.0 33.0 37.0 27.0 40.0 68-69 33.68574878705034 37.0 33.0 37.0 27.0 40.0 70-71 33.376214750620434 37.0 33.0 37.0 24.5 37.0 72-73 33.24391521598969 37.0 33.0 37.0 24.5 37.0 74-75 33.10553179783092 37.0 33.0 37.0 27.0 37.0 76-77 32.80562481765233 37.0 33.0 37.0 22.0 37.0 78-79 32.61368048726898 37.0 33.0 37.0 22.0 37.0 80-81 32.40249037384423 37.0 33.0 37.0 22.0 37.0 82-83 32.04737617467916 35.0 33.0 37.0 22.0 37.0 84-85 31.876456755238106 33.0 33.0 37.0 22.0 37.0 86-87 31.76236452243822 33.0 33.0 37.0 22.0 37.0 88-89 31.687187532642483 33.0 33.0 37.0 18.5 37.0 90-91 31.599399963620513 33.0 33.0 37.0 15.0 37.0 92-93 31.41117980290243 33.0 33.0 37.0 15.0 37.0 94-95 31.17191625334529 33.0 33.0 37.0 15.0 37.0 96-97 31.04318915711255 33.0 33.0 37.0 15.0 37.0 98-99 30.890635749507435 33.0 33.0 37.0 15.0 37.0 100-101 29.491066765359527 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2911697067468211 2306 2 0.13589843197950202 2306 3 0.18684958314522504 2306 4 0.3340114254431015 2306 5 0.3212461370882451 2306 6 0.31317246631311235 2306 7 0.39232573545081806 2306 8 0.27652634054529557 2306 9 0.20705870915525537 2306 10-11 0.3450704906004489 2306 12-13 0.3022137341061679 2306 14-15 0.72584566981741 2306 16-17 0.5615469121893213 2306 18-19 0.5227436894020556 2306 20-21 0.539414349282481 2306 22-23 0.5670104224025962 2306 24-25 0.6370826517335999 2306 26-27 0.529938925960181 2306 28-29 0.479215993519027 2306 30-31 0.5487060977834304 2306 32-33 0.4848705982467081 2306 34-35 0.47674916288616487 2306 36-37 0.5278868505756691 2306 38-39 0.4224510274436071 2306 40-41 0.3452488299874261 2306 42-43 0.4694241205713823 2306 44-45 0.6775932450508861 2306 46-47 0.5267617140751923 2306 48-49 0.5597641207312734 2306 50-51 0.5488855303091 2306 52-53 0.5911364587446855 2306 54-55 0.6723150769425601 2306 56-57 0.6099012873418985 2306 58-59 0.754982287791222 2306 60-61 0.7615534640908947 2306 62-63 0.6688985384101542 2306 64-65 0.7298871995602312 2306 66-67 0.7500264576952489 2306 68-69 0.7556460481252145 2306 70-71 0.6626357147428266 2306 72-73 0.5717418226654303 2306 74-75 0.6061950151279234 2306 76-77 0.5940769707645686 2306 78-79 0.5611341817246398 2306 80-81 0.5924303978586636 2306 82-83 0.5700490006610295 2306 84-85 0.6842204735291268 2306 86-87 0.7529388191140249 2306 88-89 0.6425196851794901 2306 90-91 0.5838956195563334 2306 92-93 0.4024443242072273 2306 94-95 0.48221335930611886 2306 96-97 0.5767394996370534 2306 98-99 0.5860059415883292 2306 100-101 0.4876056898137797 2307 1 0.1785778226992747 2307 2 0.2819394423945276 2307 3 0.17112176414211788 2307 4 0.3050822129732822 2307 5 0.27167839463621846 2307 6 0.2530158159378857 2307 7 0.37899313574376947 2307 8 0.19177345797593404 2307 9 0.3125929672104206 2307 10-11 0.3050977335305731 2307 12-13 0.3382665283934685 2307 14-15 0.612277851181112 2307 16-17 0.625709056296806 2307 18-19 0.5047051239377254 2307 20-21 0.5467451452564234 2307 22-23 0.5012619877953242 2307 24-25 0.6539465333714745 2307 26-27 0.6308538073225805 2307 28-29 0.6225849112544779 2307 30-31 0.5724277980611134 2307 32-33 0.4799784042773254 2307 34-35 0.5186080096609089 2307 36-37 0.47756590670901744 2307 38-39 0.4352498082131362 2307 40-41 0.49553129374434945 2307 42-43 0.6386835213045146 2307 44-45 0.6071585961633659 2307 46-47 0.5081819228694684 2307 48-49 0.48692543492400375 2307 50-51 0.40730306313093934 2307 52-53 0.4283888730982284 2307 54-55 0.39846384577304406 2307 56-57 0.4907027105769046 2307 58-59 0.4443702071529998 2307 60-61 0.49921628592093725 2307 62-63 0.5684405863067852 2307 64-65 0.6904882934393584 2307 66-67 0.6428778690969992 2307 68-69 0.4427946437775532 2307 70-71 0.39474995869684903 2307 72-73 0.4356518532766884 2307 74-75 0.484107608493332 2307 76-77 0.5053886063949591 2307 78-79 0.2884837345476683 2307 80-81 0.3534673292409707 2307 82-83 0.38800598547108933 2307 84-85 0.5614271279428245 2307 86-87 0.3697352988364919 2307 88-89 0.4262385426552946 2307 90-91 0.46950723286050433 2307 92-93 0.6038817026783256 2307 94-95 0.44066983075412125 2307 96-97 0.5251484652557217 2307 98-99 0.4849630283301174 2307 100-101 0.3396163372707264 2201 1 0.342892746365429 2201 2 0.4224665316013976 2201 3 0.3945000023462768 2201 4 0.513279125043077 2201 5 0.5769070637143585 2201 6 0.4697454292151946 2201 7 0.559231113827714 2201 8 0.5343319052337705 2201 9 0.7033105585716655 2201 10-11 0.6299948357700487 2201 12-13 0.6686459701190941 2201 14-15 0.8425635379197658 2201 16-17 0.7877908159648754 2201 18-19 0.7535150672857824 2201 20-21 0.7950377738536858 2201 22-23 0.735978927526169 2201 24-25 0.9791719442975833 2201 26-27 0.8598345709270276 2201 28-29 0.7808724198985573 2201 30-31 0.9040024496383197 2201 32-33 0.6014054083732887 2201 34-35 0.6991890911014025 2201 36-37 0.7960988073952251 2201 38-39 0.699117899600985 2201 40-41 0.6799716230171811 2201 42-43 0.775392255811667 2201 44-45 0.8876369460755882 2201 46-47 0.737386268845313 2201 48-49 0.8613878398391677 2201 50-51 0.8946536203414226 2201 52-53 0.9486512540759406 2201 54-55 1.0013981773864202 2201 56-57 0.9117734789688399 2201 58-59 1.1263863715766433 2201 60-61 1.0370278051506716 2201 62-63 1.0759843718804802 2201 64-65 1.0505621338032043 2201 66-67 0.8710967903162157 2201 68-69 0.9835782293406155 2201 70-71 1.0164710276366833 2201 72-73 0.899561668958377 2201 74-75 0.9453497290848532 2201 76-77 0.847708676611461 2201 78-79 0.8650100790340787 2201 80-81 0.9177977125060437 2201 82-83 0.946511923286927 2201 84-85 0.9175416486620129 2201 86-87 0.9964629505732852 2201 88-89 1.1395756882481471 2201 90-91 1.182623289565651 2201 92-93 0.9590511930767498 2201 94-95 0.8999828261262159 2201 96-97 1.059664762665161 2201 98-99 0.8491205300589328 2201 100-101 0.9638182562557773 1101 1 0.12433899858118025 1101 2 -0.008352979667275662 1101 3 0.09952342599783393 1101 4 0.014347433475592197 1101 5 0.107577803142064 1101 6 0.05300701442664035 1101 7 0.12051719891254464 1101 8 0.009422117945845798 1101 9 0.006754468422258242 1101 10-11 0.08392700214128013 1101 12-13 0.08920576373773059 1101 14-15 0.2725655954026749 1101 16-17 0.15352264680812766 1101 18-19 0.20610195637162576 1101 20-21 0.1524043540005593 1101 22-23 0.2716509330971988 1101 24-25 0.22808622639431775 1101 26-27 0.3309087352724376 1101 28-29 0.10831211982258537 1101 30-31 0.19285643165908795 1101 32-33 0.3399673860891639 1101 34-35 0.2008271640312671 1101 36-37 0.2157999975699525 1101 38-39 0.29375736528849217 1101 40-41 0.0804828283856267 1101 42-43 -0.007935801924460861 1101 44-45 -0.009055254481509678 1101 46-47 -0.04991114822229292 1101 48-49 0.0500542743795549 1101 50-51 -0.046169214553479776 1101 52-53 0.18675267215647295 1101 54-55 0.2999072936183822 1101 56-57 0.1983958833750208 1101 58-59 0.2968686139164731 1101 60-61 0.38213890922766325 1101 62-63 0.3024602201965081 1101 64-65 0.343956822331954 1101 66-67 0.32506931500601155 1101 68-69 0.28751039571185544 1101 70-71 0.24602202155919883 1101 72-73 0.15481062439013016 1101 74-75 0.17926416944950319 1101 76-77 0.2809259468064411 1101 78-79 0.28244328997355694 1101 80-81 0.3035306512247047 1101 82-83 0.20570174982528755 1101 84-85 0.17614113587396574 1101 86-87 0.2734547522952404 1101 88-89 0.2835678381595166 1101 90-91 0.28761031159654493 1101 92-93 0.09723965851103955 1101 94-95 0.12022212721509717 1101 96-97 0.21126880799309333 1101 98-99 0.040715712383569524 1101 100-101 0.27105663939074987 2304 1 0.20077470471544956 2304 2 0.050047128317459055 2304 3 0.11521986618287983 2304 4 -0.014326501709028605 2304 5 0.06274757232469597 2304 6 0.15726044723681554 2304 7 0.17696990037156723 2304 8 0.08458470859780931 2304 9 0.10347075080233736 2304 10-11 0.18357037641882812 2304 12-13 0.1283088155335932 2304 14-15 0.3260249416041887 2304 16-17 0.30501648676983706 2304 18-19 0.317187724474401 2304 20-21 0.2932518807820017 2304 22-23 0.3865020552794718 2304 24-25 0.4341729454990215 2304 26-27 0.42378005550852293 2304 28-29 0.32872859731145354 2304 30-31 0.3335666767108094 2304 32-33 0.32637787533364104 2304 34-35 0.24715414917109513 2304 36-37 0.2389852711517335 2304 38-39 0.3125565259526013 2304 40-41 0.2872228244589081 2304 42-43 0.2880474420351362 2304 44-45 0.12354002977603784 2304 46-47 0.1970128207075561 2304 48-49 0.267803038077048 2304 50-51 0.26638094312877314 2304 52-53 0.30345702099624816 2304 54-55 0.5563641365374039 2304 56-57 0.7023459158931473 2304 58-59 0.5277767900788461 2304 60-61 0.4454854543296918 2304 62-63 0.623637806001426 2304 64-65 0.6351048350446931 2304 66-67 0.6604740430194553 2304 68-69 0.54277051007751 2304 70-71 0.6101772250736133 2304 72-73 0.6216294002359106 2304 74-75 0.6373113330095563 2304 76-77 0.832510438725997 2304 78-79 0.6747392540440913 2304 80-81 0.6305868094223399 2304 82-83 0.6841733670194046 2304 84-85 0.5696329081734284 2304 86-87 0.5974103374375268 2304 88-89 0.7174641544075335 2304 90-91 0.5669419719845585 2304 92-93 0.4823316296005835 2304 94-95 0.6446013683769145 2304 96-97 0.6329064026331217 2304 98-99 0.7683173162023031 2304 100-101 0.43996838641196234 2202 1 0.24456536188338163 2202 2 0.18959722767401743 2202 3 0.2843354491373198 2202 4 0.25958123926102417 2202 5 0.24189275226063955 2202 6 0.209010128389572 2202 7 0.19127879206518372 2202 8 0.261137942272498 2202 9 0.2218664131536059 2202 10-11 0.1814007507908073 2202 12-13 0.32490466532504314 2202 14-15 0.4211874431790861 2202 16-17 0.3003541056606238 2202 18-19 0.24787644004498333 2202 20-21 0.3566492948590252 2202 22-23 0.3470646023881798 2202 24-25 0.40088601171536453 2202 26-27 0.31002743344584616 2202 28-29 0.4325711210343499 2202 30-31 0.5346216908461869 2202 32-33 0.46972264369691885 2202 34-35 0.26903497213958616 2202 36-37 0.26356676342314245 2202 38-39 0.2855280054259097 2202 40-41 0.3483510076816856 2202 42-43 0.36794174721339346 2202 44-45 0.3517676535517609 2202 46-47 0.4093261265290309 2202 48-49 0.41300286743575043 2202 50-51 0.40792093663025497 2202 52-53 0.5241991945950275 2202 54-55 0.5770122584884589 2202 56-57 0.5364315150496637 2202 58-59 0.6348895702720796 2202 60-61 0.5935189902074711 2202 62-63 0.5800074013472525 2202 64-65 0.643493501544846 2202 66-67 0.5425998920814834 2202 68-69 0.6533233315164182 2202 70-71 0.5831796498599218 2202 72-73 0.6741304722699653 2202 74-75 0.5977431914303324 2202 76-77 0.7692214713931946 2202 78-79 0.705353839012183 2202 80-81 0.6707188740715253 2202 82-83 0.5991283250111437 2202 84-85 0.6415091998639433 2202 86-87 0.6661162541813646 2202 88-89 0.6184597369641089 2202 90-91 0.5998155648016983 2202 92-93 0.7155167201487806 2202 94-95 0.6892851570115859 2202 96-97 0.6726906088084839 2202 98-99 0.7142457695334592 2202 100-101 0.7551499911916864 1102 1 0.027322836237534176 1102 2 -0.03316127690188608 1102 3 0.08275181707147539 1102 4 0.0759713994986555 1102 5 -0.03299831132884634 1102 6 0.004147434054459609 1102 7 0.05216880826255732 1102 8 0.04615570405852054 1102 9 0.08290786240851133 1102 10-11 0.11251838398989378 1102 12-13 0.07553436451937046 1102 14-15 0.002329567992354953 1102 16-17 0.0021185034864785734 1102 18-19 0.021784381146822795 1102 20-21 0.11310833209772397 1102 22-23 0.23438672220296297 1102 24-25 0.21108518314998292 1102 26-27 0.23346812606011014 1102 28-29 0.1309534344163552 1102 30-31 0.1875651440731687 1102 32-33 0.018206739171766628 1102 34-35 0.08926211218678759 1102 36-37 0.2663675595556114 1102 38-39 0.13938103475479835 1102 40-41 0.18924184424082569 1102 42-43 0.15858876462604599 1102 44-45 0.21800766850905973 1102 46-47 0.10694209324904591 1102 48-49 0.14701046341330937 1102 50-51 0.1163363458858484 1102 52-53 0.1373783539722382 1102 54-55 0.2696750526024587 1102 56-57 0.21186313503520893 1102 58-59 0.3202195464815816 1102 60-61 0.26201700321497157 1102 62-63 0.24621531726589296 1102 64-65 0.27776680888097616 1102 66-67 0.23356222412195393 1102 68-69 0.278982005697344 1102 70-71 0.34620551605701166 1102 72-73 0.34893191610666463 1102 74-75 0.3983235019849545 1102 76-77 0.3767486005658327 1102 78-79 0.22851033828053602 1102 80-81 0.26253392923607777 1102 82-83 -0.017875174881062605 1102 84-85 0.2602903092219826 1102 86-87 0.3622478192217464 1102 88-89 0.3970549485315864 1102 90-91 0.2898800299735278 1102 92-93 0.34457011806811266 1102 94-95 0.34541144693506354 1102 96-97 0.35337695589018736 1102 98-99 0.3619694325822671 1102 100-101 0.21667752863738698 2305 1 0.19320599465211075 2305 2 0.22585446405949483 2305 3 0.09955429183517239 2305 4 0.2783444582112722 2305 5 0.1924884566629217 2305 6 0.2726766487676642 2305 7 0.25340221860849255 2305 8 0.2518980640976096 2305 9 0.22755298281408898 2305 10-11 0.16405142688556396 2305 12-13 0.24881250261022103 2305 14-15 0.21351633909225853 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-0.654954571622909 1213 54-55 -0.6001551465506267 1213 56-57 -0.806089012222138 1213 58-59 -1.2757696361752977 1213 60-61 -0.9954672030710725 1213 62-63 -0.9156198975580949 1213 64-65 -0.8219030705660728 1213 66-67 -0.6813433083448146 1213 68-69 -0.6435968646222605 1213 70-71 -0.7122588584333158 1213 72-73 -0.6589735377599766 1213 74-75 -0.6092331637974624 1213 76-77 -0.8254502381172202 1213 78-79 -0.7364947952905645 1213 80-81 -0.6849396905947316 1213 82-83 -0.5457794497457229 1213 84-85 -0.6652139597476676 1213 86-87 -0.7503063411814708 1213 88-89 -0.5876118851603565 1213 90-91 -0.5801307038569483 1213 92-93 -0.5011599297797176 1213 94-95 -0.4384744207612634 1213 96-97 -0.535705363010198 1213 98-99 -0.7050439343526271 1213 100-101 -1.135780199229309 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1030.0 3 114.0 4 107.0 5 140.0 6 306.0 7 1937.0 8 9145.0 9 16262.0 10 19391.0 11 16634.0 12 11570.0 13 8568.0 14 6928.0 15 6472.0 16 6296.0 17 6217.0 18 6370.0 19 6831.0 20 7502.0 21 8284.0 22 9308.0 23 10288.0 24 11612.0 25 13598.0 26 16239.0 27 19512.0 28 23766.0 29 29004.0 30 37084.0 31 49261.0 32 67847.0 33 98767.0 34 151105.0 35 261475.0 36 485595.0 37 654996.0 38 141349.0 39 122.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.01108531452889 20.836422609351352 27.029767091137703 12.12272498498206 2 36.19083394926469 19.650562861300674 33.13348446387365 11.02511872556098 3 43.2031945932105 15.252577709976523 27.477946559627238 14.066281137185744 4 35.77997449468324 13.647237757966355 34.854020603499606 15.718767143850798 5 35.161222987299354 12.81123243241781 36.548145082429514 15.479399497853322 6 27.386200841699143 13.388319293741096 43.90669972858742 15.318780135972334 7 37.44869677177366 14.261080235003048 31.28036267505311 17.009860318170176 8 33.63003874029577 26.797970754998506 23.171421075550338 16.400569429155386 9 34.96182139444314 27.564367235814867 20.332969727566123 17.140841642175868 10-11 31.056441735763933 26.649450095040432 26.14582030541387 16.148287863781764 12-13 31.55126561934357 22.757434868402367 26.205355512647177 19.48594399960688 14-15 29.019085883658946 23.424481998861722 26.462107167208572 21.094324950270764 16-17 27.739937592053977 26.113341755062898 27.792924620261513 18.35379603262162 18-19 26.929722074224728 26.424134079421012 27.232268021812878 19.41387582454138 20-21 25.69717039258611 28.027374341208855 27.286562486055693 18.988892780149342 22-23 27.52899576755783 25.239446956081142 27.31543527347689 19.916122002884137 24-25 27.36052719740561 25.571456796062296 27.59433631711416 19.47367968941794 26-27 23.854685103037358 26.109706912645507 29.41210472766553 20.623503256651603 28-29 23.345983062982274 27.297923732797212 27.17482390419231 22.1812693000282 30-31 22.89624285770691 27.311745229540467 27.9079694735714 21.884042439181222 32-33 22.20404500306289 24.896681017340434 30.409682619433585 22.48959136016309 34-35 22.245578772146775 27.016342210778717 28.296738646075582 22.44134037099893 36-37 21.153687272884873 29.711838902831634 28.344757655831053 20.789716168452436 38-39 20.390390248010423 27.895996779560413 28.131840245064073 23.581772727365088 40-41 22.75361379809508 27.691077126191534 26.155231278788644 23.40007779692474 42-43 25.162378072443413 26.99024669901654 26.749587871192638 21.097787357347414 44-45 21.660211977500925 29.32718162398083 26.385206999330375 22.62739939918787 46-47 22.369018597829143 29.437458292920045 27.025532919094758 21.167990190156054 48-49 22.114585535146812 27.12022217765902 28.628791861555385 22.136400425638776 50-51 20.642688708538817 27.12108682171313 27.291811666107368 24.944412803640684 52-53 22.832089998010392 28.301274907074486 26.42869515396197 22.437939940953147 54-55 23.346044159074903 27.988908639876918 27.4289895785378 21.236057622510376 56-57 24.80809607186874 27.509496404892108 25.079112244773533 22.60329527846562 58-59 22.906921079372246 26.11647284633623 25.968770464039743 25.007835610251778 60-61 20.46574736309782 26.046114181476664 28.49990066464384 24.988237790781678 62-63 22.301742000123717 26.05746555033451 28.54158003172363 23.099212417818148 64-65 22.78377955504339 28.380156829153037 27.360909573866415 21.475154041937156 66-67 22.036333210362262 27.877895596205747 26.829766645459046 23.256004547972942 68-69 21.215108207818794 24.742369055523657 30.846605367302875 23.195917369354667 70-71 21.033165772888072 29.19244382218384 28.279713012767804 21.494677392160284 72-73 23.457164039481686 30.030275913665687 26.410707494953073 20.10185255189955 74-75 24.736175315692428 28.223818581157563 26.861060119864604 20.178945983285406 76-77 23.27885961344619 27.447288629763932 26.624608013563734 22.649243743226144 78-79 23.64398872806109 28.87172730321615 26.36172126822403 21.122562700498733 80-81 22.66420939893621 29.777344909426812 26.227706558895992 21.330739132740987 82-83 22.427830818272284 29.367222547507815 26.928521444876207 21.27642518934369 84-85 22.91360728943788 28.552150079318928 26.81359204665421 21.72065058458898 86-87 20.552093007187693 29.272431318597576 27.921371363336366 22.25410431087836 88-89 20.04255600689802 29.485355932922918 28.431368785532495 22.04071927464657 90-91 21.527092828712153 28.63534968542072 27.0336581431097 22.80389934275743 92-93 21.95881973595496 28.752531754001883 26.002357891657518 23.286290618385642 94-95 22.672882739519633 30.04498655910035 25.68786012888188 21.594270572498143 96-97 21.28505037793608 31.358200170258556 24.649778329859252 22.70697112194611 98-99 21.615596270009625 29.19970988261442 26.422352853802916 22.762340993573037 100-101 23.139426136308487 29.810288498459457 26.205429106553886 20.84485625867817 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 946.0 1 574.0 2 176.0 3 176.0 4 181.5 5 164.5 6 201.0 7 272.5 8 375.0 9 476.5 10 564.0 11 702.0 12 833.0 13 854.0 14 786.0 15 714.0 16 643.5 17 606.0 18 608.5 19 600.5 20 603.0 21 656.5 22 739.0 23 843.0 24 1022.5 25 1248.0 26 1478.0 27 1908.5 28 2445.5 29 2967.0 30 3743.0 31 4900.5 32 6591.0 33 9400.0 34 14189.5 35 23450.5 36 35405.5 37 48494.5 38 64797.0 39 79293.0 40 89143.0 41 96525.0 42 131827.0 43 168479.0 44 159122.0 45 138051.5 46 134592.5 47 130294.0 48 114996.0 49 103967.0 50 94210.0 51 88654.0 52 87085.0 53 87609.0 54 75128.0 55 47765.0 56 31577.0 57 25846.5 58 22001.5 59 18004.5 60 14113.0 61 11052.0 62 8495.0 63 6488.0 64 5098.0 65 3887.5 66 3006.5 67 2421.5 68 1906.5 69 1348.0 70 973.5 71 818.5 72 623.0 73 405.0 74 289.5 75 225.0 76 167.5 77 125.0 78 92.5 79 67.5 80 58.0 81 58.0 82 53.0 83 40.5 84 39.5 85 32.5 86 26.5 87 19.5 88 14.5 89 15.0 90 10.0 91 8.5 92 8.0 93 3.0 94 2.0 95 3.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16586883935035604 2 0.15861995684888827 3 0.12534713592600197 4 0.12025941094049974 5 0.13304625957662924 6 0.1519563878413278 7 0.1282286792806227 8 0.12021438682558377 9 0.11310057666886383 10-11 0.10083150535426776 12-13 0.1291741856938576 14-15 0.1246942862597207 16-17 0.11546434270195116 18-19 0.11373091427768713 20-21 0.10738251407453833 22-23 0.12115989323881872 24-25 0.4135464955029914 26-27 0.2990051471568172 28-29 0.3872073882771612 30-31 0.221496133329011 32-33 0.26136498708708383 34-35 0.27433193218287716 36-37 0.27109019590892885 38-39 0.2893924986222621 40-41 0.283269218993693 42-43 0.3388064647425161 44-45 0.28648844321018335 46-47 0.3119946043100685 48-49 0.31282755043601357 50-51 0.3126024298614338 52-53 0.3164069675718315 54-55 0.2933546207348656 56-57 0.30494833032572244 58-59 0.305353547359966 60-61 0.28419221334947 62-63 0.2827064175572437 64-65 0.2906981979548246 66-67 0.2890097936454765 68-69 0.28732138933612844 70-71 0.2616576438340375 72-73 0.2509869285989576 74-75 0.260351944501475 76-77 0.25722276851481657 78-79 0.2529905017127173 80-81 0.2626931984771043 82-83 0.3033949983611222 84-85 0.30929315741511154 86-87 0.2981722010308721 88-89 0.3049708423831804 90-91 0.28133318205230723 92-93 0.2664302000151281 94-95 0.2517973626674447 96-97 0.25114451300116347 98-99 0.2731387931376045 100-101 0.2730037207928567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2221032.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.41751991394709 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28501187624737 40.5155377695482 2 5.792892865352965 5.377834398737667 3 2.0605990163430175 2.8694368762728546 4 1.120156225713519 2.079794956551563 5 0.6323069958516041 1.4675061285834947 6 0.4362226548532463 1.2149024261139256 7 0.37142407697421775 1.206840914262912 8 0.24787485330087494 0.920458875140806 9 0.23107751340959173 0.9653440572319592 >10 1.4748165673774394 13.43623814137663 >50 0.19106813555811808 6.170829907291578 >100 0.1422077250477912 12.859476522279218 >500 0.009027851809675105 2.7748174038213724 >1k 0.0045291695577187666 3.4157677793477315 >5k 2.941772260576712E-4 0.7538002607196012 >10k+ 4.902953767245654E-4 3.9714135827202597 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 31637 1.4244279235958779 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 21669 0.9756275461136985 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12276 0.5527160347081896 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 11548 0.5199384790493788 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10122 0.4557340911792356 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5779 0.26019436009926916 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5619 0.2529905017127173 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5163 0.2324595053110446 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 4242 0.1909922954734556 Illumina Single End PCR Primer 1 (96% over 32bp) GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 3034 0.13660316465498923 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 2931 0.13196568081864646 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 2719 0.12242056845646529 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2604 0.11724279524113115 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2498 0.11247023906004056 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2368 0.10661710412096718 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2338 0.10526638067348872 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2237 0.10071894506697787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002476326320377194 0.0 0.0 2 0.002566374550209092 0.0 0.0 3 0.002836519239704786 0.0 0.0 4 0.002881543354620735 0.0 0.0 5 0.0036919774231078165 0.0 0.0 6 0.0040971944573513575 0.0 0.0 7 0.004592459721426796 0.0 0.0 8 0.0052678214451660306 0.0 0.0 9 0.005943183168905266 0.0 0.0 10-11 0.009882793224050801 0.0 0.0 12-13 0.013372162130036848 0.0 0.0 14-15 0.01701911543822872 0.0 0.0 16-17 0.020958725493374252 0.0 0.0 18-19 0.025911378134128638 0.0 0.0 20-21 0.02892799383349722 0.0 0.0 22-23 0.032102193935071625 0.0 0.0 24-25 0.035816683415637415 0.0 0.0 26-27 0.04025155873485839 0.0 0.0 28-29 0.04538430783527657 0.0 0.0 30-31 0.0965542144372526 0.0 0.0 32-33 0.10452348277737555 0.0 0.0 34-35 0.11062425034848665 0.0 0.0 36-37 0.11749042787316888 0.0 0.0 38-39 0.1261350579370311 0.0 0.0 40-41 0.13595031498870797 0.0 0.0 42-43 0.14657600610887192 0.0 0.0 44-45 0.1596329994344971 0.0 0.0 46-47 0.17255492041537446 0.0 0.0 48-49 0.1885159691530784 0.0 0.0 50-51 0.2068632959813276 0.0 0.0 52-53 0.22827226262386136 0.0 0.0 54-55 0.25105446477133153 0.0 0.0 56-57 0.279779850087707 0.0 0.0 58-59 0.31131924258632926 0.0 0.0 60-61 0.34596529901415196 0.0 0.0 62-63 0.38635193009375823 0.0 0.0 64-65 0.4292373995511996 0.0 0.0 66-67 0.48002460117639006 0.0 0.0 68-69 0.5309243630888705 0.0 0.0 70-71 0.587744796112798 0.0 0.0 72-73 0.6494503456051062 0.0 0.0 74-75 0.7182246811392181 0.0 0.0 76-77 0.7958012311393983 0.0 0.0 78-79 0.8854892680519686 0.0 0.0 80-81 0.9841145917753549 0.0 0.0 82-83 1.0972151684442186 0.0 0.0 84-85 1.224858534230934 0.0 0.0 86-87 1.3667295203310892 0.0 0.0 88-89 1.5208695777458408 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 970 0.0 72.36483 9 AGAGCGG 1130 0.0 63.377632 8 CGGGAGA 1410 0.0 62.90845 4 AAGCGGC 395 0.0 62.437256 9 CGAGTCG 25 0.0050452463 56.933216 1 ATCGGGA 1865 0.0 56.72458 2 TCGGGAG 1585 0.0 56.561256 3 GTTCGGA 150 0.0 53.765404 2 GATCCGG 305 0.0 52.88878 1 TTGTGTT 28880 0.0 52.224922 1 ATCCGGA 275 0.0 51.752796 2 TCGGGAA 435 0.0 49.069286 3 TCGGAGA 360 0.0 48.751247 4 TCGGAAA 520 0.0 48.34583 4 ATTCGGA 335 0.0 48.14812 2 GGTTCGG 220 0.0 47.444344 1 CGGAAAG 640 0.0 47.433643 5 GGATCGG 650 0.0 45.98452 1 ACCGGAA 130 0.0 43.7849 3 GGAGAGC 2105 0.0 41.236847 6 >>END_MODULE