FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n02_30230-1.34200000035711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n02_30230-1.34200000035711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221032
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA316371.4244279235958779No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC216690.9756275461136985No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT122760.5527160347081896No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT115480.5199384790493788No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC101220.4557340911792356No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT57790.26019436009926916No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT56190.2529905017127173No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC51630.2324595053110446No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT42420.1909922954734556Illumina Single End PCR Primer 1 (96% over 32bp)
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC30340.13660316465498923No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT29310.13196568081864646No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC27190.12242056845646529No Hit
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC26040.11724279524113115No Hit
TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA24980.11247023906004056No Hit
GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT23680.10661710412096718No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG23380.10526638067348872No Hit
AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA22370.10071894506697787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9700.072.364839
AGAGCGG11300.063.3776328
CGGGAGA14100.062.908454
AAGCGGC3950.062.4372569
CGAGTCG250.005045246356.9332161
ATCGGGA18650.056.724582
TCGGGAG15850.056.5612563
GTTCGGA1500.053.7654042
GATCCGG3050.052.888781
TTGTGTT288800.052.2249221
ATCCGGA2750.051.7527962
TCGGGAA4350.049.0692863
TCGGAGA3600.048.7512474
TCGGAAA5200.048.345834
ATTCGGA3350.048.148122
GGTTCGG2200.047.4443441
CGGAAAG6400.047.4336435
GGATCGG6500.045.984521
ACCGGAA1300.043.78493
GGAGAGC21050.041.2368476
Produced by FastQC (version 0.11.2)