FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4725086
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT54245911.4804048010978TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC86710.18350988743908578No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT75370.15951032425653205TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG67240.1423042882182462No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA65030.13762712467032345No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC60250.12751090667979376No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG49950.10571236163743898No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT49830.10545839800587757No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG47470.10046377991850307TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA686150.087.314191
TCGGAAG686300.087.056883
CGGAAGA686650.086.618244
ATCGGAA691700.086.3772352
GAAGAGC691150.086.006276
GGAAGAG711900.083.666175
AGAGCAC866200.068.636278
GAGCACA866450.068.556179
AAGAGCA872750.068.077627
TCGGACG2450.058.1556053
CGGACCG504.2013744E-757.084931
TCGCCGG903.6379788E-1252.770832
ATCGGAC3000.049.076872
GTGCGAC405.3504726E-447.5707781
GCCGTCT645750.046.2063850-51
ATGCCGT645600.046.15458348-49
CGTCTTC645950.046.13348452-53
TCGTATG649500.046.04760744-45
TCTCGTA635650.046.0422942-43
AATCTCG630000.046.0004840-41
Produced by FastQC (version 0.11.2)