##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5259731 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68853236030512 33.0 33.0 33.0 33.0 33.0 2 32.71541282244282 33.0 33.0 33.0 33.0 33.0 3 32.74910237804937 33.0 33.0 33.0 33.0 33.0 4 36.67524860111667 37.0 37.0 37.0 37.0 37.0 5 36.69182131177431 37.0 37.0 37.0 37.0 37.0 6 36.56719478619724 37.0 37.0 37.0 37.0 37.0 7 36.46416042949725 37.0 37.0 37.0 37.0 37.0 8 36.38982164677243 37.0 37.0 37.0 37.0 37.0 9 36.47553002235286 37.0 37.0 37.0 37.0 37.0 10-11 36.39835639883485 37.0 37.0 37.0 37.0 37.0 12-13 36.381801179566025 37.0 37.0 37.0 37.0 37.0 14-15 38.266900056295654 40.0 37.0 40.0 37.0 40.0 16-17 38.288707160119024 40.0 37.0 40.0 37.0 40.0 18-19 38.23140128268918 40.0 37.0 40.0 37.0 40.0 20-21 38.255900919647786 40.0 37.0 40.0 37.0 40.0 22-23 38.0645557729093 40.0 37.0 40.0 37.0 40.0 24-25 38.138742456600916 40.0 37.0 40.0 37.0 40.0 26-27 38.10000131185416 40.0 37.0 40.0 37.0 40.0 28-29 37.90715038468697 40.0 37.0 40.0 33.0 40.0 30-31 37.94148617106084 40.0 37.0 40.0 35.0 40.0 32-33 37.813975182381 40.0 37.0 40.0 33.0 40.0 34-35 37.65303691386499 40.0 37.0 40.0 33.0 40.0 36-37 37.31024523877742 40.0 37.0 40.0 33.0 40.0 38-39 37.53199878472873 40.0 37.0 40.0 33.0 40.0 40-41 37.309371239707886 40.0 37.0 40.0 33.0 40.0 42-43 37.128595739972255 40.0 37.0 40.0 33.0 40.0 44-45 37.18892591655353 40.0 37.0 40.0 33.0 40.0 46-47 37.22621822294714 40.0 37.0 40.0 33.0 40.0 48-49 36.93070624714458 37.0 37.0 40.0 33.0 40.0 50-51 36.89361404604152 37.0 37.0 40.0 33.0 40.0 52-53 36.8525789626884 37.0 37.0 40.0 33.0 40.0 54-55 36.5581527838591 37.0 37.0 40.0 33.0 40.0 56-57 36.453754574140774 37.0 37.0 40.0 33.0 40.0 58-59 36.204630997288646 37.0 37.0 40.0 33.0 40.0 60-61 35.92857828660819 37.0 37.0 40.0 33.0 40.0 62-63 35.74281821636886 37.0 37.0 40.0 33.0 40.0 64-65 35.500582729420955 37.0 33.0 40.0 30.0 40.0 66-67 34.87591009882445 37.0 33.0 37.0 27.0 40.0 68-69 34.700712736069576 37.0 33.0 37.0 27.0 40.0 70-71 34.262708777312 37.0 33.0 37.0 27.0 40.0 72-73 33.48925781565635 37.0 33.0 37.0 24.5 40.0 74-75 32.571417910915976 37.0 33.0 37.0 22.0 38.5 76-77 30.06230708376531 33.0 30.0 35.0 18.5 37.0 78-79 32.04866370542524 35.0 33.0 37.0 18.5 37.0 80-81 32.28561346578371 37.0 33.0 37.0 15.0 37.0 82-83 32.16958129607769 37.0 33.0 37.0 15.0 37.0 84-85 31.991744539787298 37.0 33.0 37.0 15.0 37.0 86-87 31.798739897534684 37.0 33.0 37.0 15.0 37.0 88-89 31.682148288572172 37.0 33.0 37.0 15.0 37.0 90-91 31.543955574914385 35.0 33.0 37.0 15.0 37.0 92-93 31.339993052876657 33.0 33.0 37.0 15.0 37.0 94-95 31.23259269342862 33.0 33.0 37.0 15.0 37.0 96-97 31.08690178261968 33.0 33.0 37.0 15.0 37.0 98-99 30.98950782844218 33.0 33.0 37.0 15.0 37.0 100-101 29.5908703315816 33.0 30.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14534365751060818 2306 2 0.13494422067336842 2306 3 0.12064978715737595 2306 4 0.1734663950161277 2306 5 0.1461755994989602 2306 6 0.17152108589412052 2306 7 0.22653006076195936 2306 8 0.273972812045983 2306 9 0.20132368747016471 2306 10-11 0.17188692325074584 2306 12-13 0.18478387692306342 2306 14-15 0.4447757439647262 2306 16-17 0.4415982864895298 2306 18-19 0.5426090369456134 2306 20-21 0.4885782273435879 2306 22-23 0.4377579669101408 2306 24-25 0.5058258079812887 2306 26-27 0.5315401219468825 2306 28-29 0.5376327664036751 2306 30-31 0.5691287575055313 2306 32-33 0.5930833820581896 2306 34-35 0.5266040207920852 2306 36-37 0.49937729990498525 2306 38-39 0.6368414721713691 2306 40-41 0.5635549464433751 2306 42-43 0.5218396745882927 2306 44-45 0.7115448448061485 2306 46-47 0.6522636399230137 2306 48-49 0.6865223499282038 2306 50-51 0.6090385418819224 2306 52-53 0.6370050972829446 2306 54-55 0.5712052820769102 2306 56-57 0.6453295318622665 2306 58-59 0.6854874639169566 2306 60-61 0.612961805151123 2306 62-63 0.5504888743781464 2306 64-65 0.49563822497007237 2306 66-67 0.5871260078626861 2306 68-69 0.6060878976128024 2306 70-71 0.5236013223990881 2306 72-73 0.1889629549394911 2306 74-75 0.22024639761220044 2306 76-77 -0.19296193395683048 2306 78-79 0.31105260213684005 2306 80-81 0.46622062109726414 2306 82-83 0.46296028768092157 2306 84-85 0.4663376623882982 2306 86-87 0.46610295606855345 2306 88-89 0.5002078208974972 2306 90-91 0.43538100169617877 2306 92-93 0.3681293600576261 2306 94-95 0.2901111877352527 2306 96-97 0.3052083033858999 2306 98-99 0.5206218724919403 2306 100-101 0.5409389157554294 2307 1 0.1418015425193957 2307 2 0.13533536842216165 2307 3 0.12194953932166896 2307 4 0.1432608773507198 2307 5 0.12717658249204078 2307 6 0.15002663278364992 2307 7 0.23641198773202632 2307 8 0.250204722506318 2307 9 0.24143322098490927 2307 10-11 0.21138639380080093 2307 12-13 0.2189245170451244 2307 14-15 0.39128198160614147 2307 16-17 0.3628129091757941 2307 18-19 0.4795551924558836 2307 20-21 0.45390787160636137 2307 22-23 0.45616828871703063 2307 24-25 0.43146074940022316 2307 26-27 0.44428851746686604 2307 28-29 0.5529750871023253 2307 30-31 0.4334100529310092 2307 32-33 0.5108934708311992 2307 34-35 0.5137500009576357 2307 36-37 0.45195171703563375 2307 38-39 0.5153037142305692 2307 40-41 0.5765518228784856 2307 42-43 0.4752137319207037 2307 44-45 0.6265621705336173 2307 46-47 0.6289331910481408 2307 48-49 0.5355232646051675 2307 50-51 0.5074147056280793 2307 52-53 0.43832746967411396 2307 54-55 0.7021512705922177 2307 56-57 0.6384882036450534 2307 58-59 0.6777623907617709 2307 60-61 0.5535315616041387 2307 62-63 0.547739264349346 2307 64-65 0.5557432420301254 2307 66-67 0.47549059582159003 2307 68-69 0.5151066883415538 2307 70-71 0.4486211530510076 2307 72-73 0.2772475750828747 2307 74-75 0.23130358959188868 2307 76-77 0.21239059620663525 2307 78-79 0.5772514931384691 2307 80-81 0.5728525983555812 2307 82-83 0.5326802674897166 2307 84-85 0.48642867464344874 2307 86-87 0.492219564097212 2307 88-89 0.41830798751292164 2307 90-91 0.32629973966969317 2307 92-93 0.45516284634316406 2307 94-95 0.45842761282852607 2307 96-97 0.5322140836888458 2307 98-99 0.6048700345985054 2307 100-101 0.3233858474127409 2201 1 0.14010211818456497 2201 2 0.1317615208709526 2201 3 0.12247370362918275 2201 4 0.11696776358791539 2201 5 0.10288232634653127 2201 6 0.18219667093590175 2201 7 0.21107335657286086 2201 8 0.1945941980194803 2201 9 0.18705247460739827 2201 10-11 0.18407210642184424 2201 12-13 0.21453300230599126 2201 14-15 0.36162404095387046 2201 16-17 0.34739251129784066 2201 18-19 0.36453827000161 2201 20-21 0.39133422277564023 2201 22-23 0.3120793909498687 2201 24-25 0.3929093905578256 2201 26-27 0.4211955318839813 2201 28-29 0.36703929566935756 2201 30-31 0.280874910096756 2201 32-33 0.40987312955216026 2201 34-35 0.38316265185775933 2201 36-37 0.3471254101883119 2201 38-39 0.3396306396084299 2201 40-41 0.4158400081650555 2201 42-43 0.4502597445576626 2201 44-45 0.5083302418732316 2201 46-47 0.5059930433707294 2201 48-49 0.4357196961678014 2201 50-51 0.507382660648581 2201 52-53 0.3116518970001678 2201 54-55 0.3713676980139766 2201 56-57 0.48209933989968334 2201 58-59 0.43734690887499283 2201 60-61 0.3522900267328595 2201 62-63 0.420679568089966 2201 64-65 0.3947719462164656 2201 66-67 0.4404990388413452 2201 68-69 0.38219607822802715 2201 70-71 0.3298233391844789 2201 72-73 0.15998802427441916 2201 74-75 0.09737076544080026 2201 76-77 0.04929756900689952 2201 78-79 0.5675583030087381 2201 80-81 0.4876156579852591 2201 82-83 0.4370649154475217 2201 84-85 0.5035773165293591 2201 86-87 0.4601648046374436 2201 88-89 0.3217868445891945 2201 90-91 0.2196201301606564 2201 92-93 0.32606236348358664 2201 94-95 0.363244763967419 2201 96-97 0.272213397010713 2201 98-99 0.3222103221085604 2201 100-101 0.3720408426319999 1101 1 0.09588128743523328 1101 2 0.07598035110014223 1101 3 0.062096672267692554 1101 4 0.07830704513445141 1101 5 0.10948137543262959 1101 6 0.08894709271223178 1101 7 0.037180144337682464 1101 8 0.12533619093763804 1101 9 0.12199555980955523 1101 10-11 0.15762789759421736 1101 12-13 0.08783406058114451 1101 14-15 0.17264357269878872 1101 16-17 0.23314080585442554 1101 18-19 0.24255388739546646 1101 20-21 0.26279680304651265 1101 22-23 0.224107184842957 1101 24-25 0.2696733491870802 1101 26-27 0.2549836501280609 1101 28-29 0.2063008370034396 1101 30-31 0.26686126654943365 1101 32-33 0.24034505225206715 1101 34-35 0.24957858666747512 1101 36-37 0.25395651961009236 1101 38-39 0.2898476960430969 1101 40-41 0.22460028583724778 1101 42-43 0.20201520097633363 1101 44-45 0.2937744573692598 1101 46-47 0.3578825346384562 1101 48-49 0.30565521273543794 1101 50-51 0.20188456447690584 1101 52-53 0.2904023721236939 1101 54-55 0.31290049654159446 1101 56-57 0.26244889745131417 1101 58-59 0.26394115671276097 1101 60-61 0.15413450629754522 1101 62-63 0.17936816045731518 1101 64-65 0.2522788980239383 1101 66-67 0.14517158703721122 1101 68-69 0.2234888143394187 1101 70-71 0.027998864821142888 1101 72-73 -0.10550033603259124 1101 74-75 -5.106837359889482E-4 1101 76-77 -0.024429600057057854 1101 78-79 0.2323004693053079 1101 80-81 0.22479799205878237 1101 82-83 0.21283594924964433 1101 84-85 0.3107731874557942 1101 86-87 0.2811722665775491 1101 88-89 0.2965622862040185 1101 90-91 0.25924709983944183 1101 92-93 0.18042249711833236 1101 94-95 0.08659350130579568 1101 96-97 0.1738218151994424 1101 98-99 0.10382287470556761 1101 100-101 0.1553358326627432 2304 1 0.07004200992221143 2304 2 0.06743459362267146 2304 3 0.0715537628216012 2304 4 0.0826014550643066 2304 5 0.06342199924575453 2304 6 0.08243912848173096 2304 7 0.06204728395128001 2304 8 0.0851412458698988 2304 9 0.06180266621152697 2304 10-11 0.1288414343372395 2304 12-13 0.06728655843033238 2304 14-15 0.15189729976644628 2304 16-17 0.23946275168268727 2304 18-19 0.274650147529222 2304 20-21 0.31356319331921867 2304 22-23 0.23448863807822562 2304 24-25 0.24889732070306536 2304 26-27 0.244085355897397 2304 28-29 0.17972469374801392 2304 30-31 0.24961090130465635 2304 32-33 0.2586162546997812 2304 34-35 0.26896321968079917 2304 36-37 0.15825194603394976 2304 38-39 0.27395019190551295 2304 40-41 0.3491386358457049 2304 42-43 0.2676424563223918 2304 44-45 0.31425237588525334 2304 46-47 0.35373838100370136 2304 48-49 0.40740663189968274 2304 50-51 0.322438165591727 2304 52-53 0.38846225470939544 2304 54-55 0.33705923224483314 2304 56-57 0.47065948913398614 2304 58-59 0.5609756740767864 2304 60-61 0.3038105681410386 2304 62-63 0.3833092925174455 2304 64-65 0.40730550706463475 2304 66-67 0.2512780361372009 2304 68-69 0.2450388568011448 2304 70-71 0.13100140328388932 2304 72-73 -0.03714490319558905 2304 74-75 -0.010924849596392505 2304 76-77 -0.0756562772952627 2304 78-79 0.15184580077938392 2304 80-81 0.1105156044807103 2304 82-83 0.00446281093530132 2304 84-85 0.09433259829587115 2304 86-87 0.06978550077499435 2304 88-89 0.12138821160629121 2304 90-91 0.01963941107881695 2304 92-93 0.06532072296666414 2304 94-95 0.047983917245897345 2304 96-97 -0.15828082112395236 2304 98-99 -0.10550937190326692 2304 100-101 0.011561904624972641 2202 1 0.028111920877520902 2202 2 0.05820352922949468 2202 3 0.07370331699171828 2202 4 0.07479828123296528 2202 5 0.052729855483086396 2202 6 0.08417531109073906 2202 7 0.04870670641641084 2202 8 0.03502394107673723 2202 9 0.09797686775745262 2202 10-11 0.083494248811526 2202 12-13 0.10979138720889381 2202 14-15 0.1099102604920148 2202 16-17 0.06502960080509723 2202 18-19 0.07544714752562953 2202 20-21 0.061676103627569034 2202 22-23 -0.020727007853430734 2202 24-25 -0.05711418125863332 2202 26-27 -0.05740921549974587 2202 28-29 0.052423178116612235 2202 30-31 0.022987302904667217 2202 32-33 0.06188469068273861 2202 34-35 0.05540181528703414 2202 36-37 0.10282531411078111 2202 38-39 0.09703785914856411 2202 40-41 0.008477246861019694 2202 42-43 0.1466567377089092 2202 44-45 0.13449217329019092 2202 46-47 0.20115336156041508 2202 48-49 0.04492404293011276 2202 50-51 0.06555491990275897 2202 52-53 -0.00920110437834154 2202 54-55 0.03198983483395068 2202 56-57 0.06215400264367332 2202 58-59 -0.021886579415678398 2202 60-61 -0.05338670320971772 2202 62-63 6.711737384534899E-4 2202 64-65 0.01988935773264444 2202 66-67 0.10108895089957315 2202 68-69 -0.18389770378538373 2202 70-71 -0.2960792027922565 2202 72-73 -0.14203079737345803 2202 74-75 -0.13330601423134425 2202 76-77 0.011549244396132252 2202 78-79 0.06640278670639077 2202 80-81 0.052876569150342334 2202 82-83 -0.018863545581403685 2202 84-85 0.09857591415546096 2202 86-87 0.0625050782296519 2202 88-89 0.025183175342558428 2202 90-91 0.013889891200125959 2202 92-93 0.05735320389343812 2202 94-95 0.11018470457042184 2202 96-97 0.08230889767808591 2202 98-99 -0.07283124186654533 2202 100-101 0.07228650671074277 1102 1 0.06656084435259402 1102 2 0.05057568387115907 1102 3 0.037209494237067986 1102 4 0.04003651340085668 1102 5 -0.0029349942963676767 1102 6 -0.032842537707956865 1102 7 0.02413604550764603 1102 8 0.04776194217559748 1102 9 0.035051636560048394 1102 10-11 -0.04740218348268144 1102 12-13 0.030567766305871658 1102 14-15 -0.042179534215989634 1102 16-17 -0.02417074631193117 1102 18-19 0.0751810972142053 1102 20-21 0.02230418775723564 1102 22-23 -0.01861802157893777 1102 24-25 -0.02004798126581875 1102 26-27 -0.00509053408232063 1102 28-29 -0.1014460085717559 1102 30-31 -0.05024826041594821 1102 32-33 -0.04831261017427124 1102 34-35 -0.05093165839934244 1102 36-37 -0.04080109605214943 1102 38-39 0.012371278918834605 1102 40-41 -0.01602842588361142 1102 42-43 -0.0336218737716365 1102 44-45 0.06445322532179176 1102 46-47 0.043292136483778165 1102 48-49 -0.017769798310062868 1102 50-51 -0.1260133391718412 1102 52-53 -0.1346515285183827 1102 54-55 -0.13110398047513883 1102 56-57 -0.1693693006678032 1102 58-59 -0.11764078230456221 1102 60-61 -0.1766199879329733 1102 62-63 -0.24546949209978663 1102 64-65 -0.30045174508280326 1102 66-67 -0.18026133947717682 1102 68-69 -0.23080824669489886 1102 70-71 -0.24775384347991292 1102 72-73 -0.04935526835375725 1102 74-75 -0.26952168764351825 1102 76-77 -0.12887204579062583 1102 78-79 0.052656586508184944 1102 80-81 -0.10677110834917869 1102 82-83 -0.07136531711061878 1102 84-85 -0.1571661511908573 1102 86-87 -0.007774085249121043 1102 88-89 -0.03869361094939805 1102 90-91 -0.12573581548598511 1102 92-93 -0.12946369829247928 1102 94-95 -0.1670369920410799 1102 96-97 -0.3910045551607233 1102 98-99 -0.3569627047388835 1102 100-101 -0.3235754962982895 2305 1 0.027235868476211067 2305 2 0.011263579320377914 2305 3 0.011470971573821487 2305 4 0.03782847392231048 2305 5 0.049246735868834435 2305 6 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-0.3974279082907728 1213 94-95 -0.5200160337892008 1213 96-97 -0.5004188369571487 1213 98-99 -0.4406829086261972 1213 100-101 -0.10416808506758457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 26.0 10 22.0 11 30.0 12 39.0 13 100.0 14 247.0 15 704.0 16 1462.0 17 2719.0 18 4302.0 19 6294.0 20 8744.0 21 12053.0 22 16350.0 23 21773.0 24 29424.0 25 39067.0 26 54621.0 27 84257.0 28 150618.0 29 219100.0 30 150405.0 31 102618.0 32 130267.0 33 186555.0 34 286772.0 35 501009.0 36 1003241.0 37 1668674.0 38 577849.0 39 386.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.93296411487536 24.29600515174376 23.006755380141033 12.764275353239851 2 26.816333382828894 29.823255219706102 32.04599246615464 11.314418931310366 3 34.42959345259292 18.132372168842856 32.738518376700256 14.699516001863975 4 25.380059400830866 18.430515879045014 33.5737725118177 22.615652208306418 5 36.839520575330894 17.417501802852282 31.71421069672707 14.028766925089753 6 30.525990777855373 17.972877320151927 37.29114663848779 14.20998526350492 7 29.85428342247921 26.58807075875173 28.3854820712314 15.17216374753766 8 25.35796982773454 34.024553727177306 26.19042684882554 14.427049596262624 9 36.508406988874526 23.205464309866798 24.43946277860978 15.846665922648896 10-11 26.30304667672168 30.93456680579292 28.063155701308677 14.69923081617672 12-13 22.386296941801774 29.21949810741272 27.458885254778238 20.93531969600727 14-15 21.30196392172908 30.07216528754037 28.037878743228507 20.58799204750205 16-17 24.680482709096722 26.53003547139578 28.7082742444433 20.0812075750642 18-19 19.071041846056385 26.90452230351704 33.34680994142096 20.677625909005613 20-21 23.60818452502609 26.969278466902587 33.035719887576 16.386817120495326 22-23 18.90790712619071 36.09009419232117 28.599568322823423 16.4024303586647 24-25 18.92249812775596 26.970685382959697 33.065825989960324 21.040990499324014 26-27 18.993956154791945 27.09913301649837 28.235198720238735 25.671712108470945 28-29 22.984701955397092 31.65911066254516 28.229748535308264 17.126438846749483 30-31 18.061638538127152 27.753997213510218 32.45541920548537 21.728945042877264 32-33 17.869782502493738 32.28417679095616 28.37609853622932 21.469942170320778 34-35 22.62217935130154 32.31450782565942 28.600248701617282 16.463064121421752 36-37 18.03831601050188 32.311413979959426 33.230243045659684 16.42002696387901 38-39 18.410284009662483 27.80047878899715 32.639078829819994 21.150158371520373 40-41 22.803967021794776 27.646272970872765 32.38415408515221 17.16560592218025 42-43 18.29819468247157 32.178025943935026 32.662855000290435 16.86092437330297 44-45 17.931490396726907 27.850138403719054 32.88029936803335 21.338071831520683 46-47 22.547256479549603 27.758397719110324 28.32263120610255 21.371714595237528 48-49 18.47039283078517 32.35140134957062 32.119824469687615 17.058381349956598 50-51 22.95481550287138 27.676969674672726 31.82507616339364 17.543138659062258 52-53 18.22868288616605 27.579094160398377 27.649609451577945 26.54261350185762 54-55 22.796504545224614 27.5492033258242 32.024160566368344 17.630131562582843 56-57 18.399585216186 27.459279894071532 31.91307149011637 22.2280633996261 58-59 18.42815645317975 27.54206656658413 32.343480310904326 21.686296669331796 60-61 22.619864228327053 27.927575509371984 32.0801131601763 17.372447102124664 62-63 18.1556256514048 28.074937256461475 31.993961561281893 21.775475530851836 64-65 22.67913378565287 27.85543344373216 32.05887911918243 17.406553651432542 66-67 18.49658545686185 36.3115745295821 27.476562427939527 17.71527758561652 68-69 18.36899528538517 36.52028615786232 27.468663146357525 17.642055410394978 70-71 18.002096751461725 37.01444388631666 27.541317358663754 17.44214200355786 72-73 17.888527132977107 36.88752098052747 27.474447046437156 17.749504840058275 74-75 18.38100476714338 35.85666312136054 27.48050652229815 18.281825589197922 76-77 18.882496329894334 34.89061382957054 27.80624961130828 18.42064022922684 78-79 18.892711022281187 34.14984649614655 28.279630198849077 18.67781228272319 80-81 18.47069702067315 34.17545755095747 28.143255714193632 19.210589714175754 82-83 18.0774553061292 33.85121740959029 28.27051556380653 19.80081172047398 84-85 18.189147196615973 33.13957164034613 28.892244217880513 19.779036945157387 86-87 18.115203667116802 32.9314817466652 29.47318285816109 19.48013172805691 88-89 18.412036930948112 33.2353525942155 29.00659339948824 19.34601707534815 90-91 18.755752828126198 32.722005565310056 28.74990266364559 19.772338942918157 92-93 18.38869058894323 33.11714962339493 28.688759359972977 19.80540042768886 94-95 18.15111220490786 33.326977858603776 28.545556106257703 19.976353830230657 96-97 18.61815867717102 33.45853264517297 28.20678969083184 19.716518986824163 98-99 18.886589919547696 32.727239014946576 28.07541084304241 20.310760222463315 100-101 18.808613994779588 32.70514119656059 27.83667986999459 20.64956493866524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 454.0 1 317.0 2 264.5 3 642.5 4 805.0 5 541.0 6 413.0 7 415.0 8 389.0 9 356.5 10 364.0 11 386.0 12 383.5 13 388.5 14 430.0 15 518.0 16 646.5 17 844.5 18 1211.0 19 1765.5 20 2647.0 21 4065.5 22 6309.5 23 9668.5 24 13989.0 25 19899.0 26 28060.0 27 37921.0 28 49261.5 29 61550.0 30 73985.5 31 86969.0 32 103105.5 33 124721.5 34 152564.0 35 191852.0 36 249394.5 37 321246.5 38 385458.0 39 406673.5 40 404696.5 41 376172.0 42 325135.5 43 301005.5 44 273538.5 45 243313.5 46 235878.0 47 206665.5 48 146337.5 49 99996.0 50 73407.5 51 56162.5 52 44228.0 53 33068.5 54 24733.0 55 18922.0 56 14241.5 57 10614.0 58 8143.5 59 6275.0 60 4648.0 61 3483.0 62 2630.5 63 1917.0 64 1363.5 65 880.5 66 532.0 67 330.5 68 212.0 69 162.0 70 123.5 71 79.5 72 55.0 73 35.0 74 22.0 75 21.5 76 21.0 77 14.0 78 5.0 79 3.0 80 2.0 81 1.0 82 0.5 83 2.5 84 2.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1505210057320422 2 0.0 3 0.0 4 0.016768918410466238 5 1.9012379150188478E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.5666711852754447E-4 24-25 0.0 26-27 0.0 28-29 1.2358046447622512E-4 30-31 0.001273829403062628 32-33 0.0011692613177365916 34-35 2.7567949767773293E-4 36-37 0.001939262673319225 38-39 0.007994705432654256 40-41 0.01757694452434925 42-43 0.006682851271291251 44-45 0.0022434607397222406 46-47 5.513589953554659E-4 48-49 0.006739888408741816 50-51 0.0028613630621033663 52-53 0.011179278940310827 54-55 0.023670412041984657 56-57 0.020685468515405064 58-59 0.020371764259426956 60-61 0.025552637577853313 62-63 0.008070754949255009 64-65 0.011730637935666293 66-67 0.029668817663869123 68-69 0.040753034708429 70-71 0.039431674357490905 72-73 0.03006807762602308 74-75 0.039450686736641094 76-77 0.03247314358852192 78-79 0.014116691519014946 80-81 0.011217303698611203 82-83 0.005418528057803716 84-85 0.008279891119907084 86-87 0.02513436523654917 88-89 0.023090534477903907 90-91 0.017196696941345478 92-93 0.03237808169277098 94-95 0.0341652453328887 96-97 0.03513487666954831 98-99 0.02540053854465181 100-101 0.025372019975926526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5259731.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.356611525837714 #Duplication Level Percentage of deduplicated Percentage of total 1 92.95897568930646 47.74058002315504 2 3.6307425243196607 3.729252667436484 3 1.0354864536586386 1.5953722662244219 4 0.5431452072523018 1.1157638964390832 5 0.30724045849409665 0.7889414435950796 6 0.15309900755075095 0.47175877534651267 7 0.1427728867469672 0.5132632176760493 8 0.13504714371573773 0.5548450957986496 9 0.11570448411166775 0.5347971218088345 >10 0.7606586678405035 7.777852247327688 >50 0.09566970849332142 3.4844384489011446 >100 0.0954286798599202 10.278869168430216 >500 0.0176730253771379 6.347357634700505 >1k 0.008056954413429393 6.171432963834924 >5k 2.6172085100107935E-4 0.864547963705156 >10k+ 3.7388008456757994E-5 8.030927065620102 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 418251 7.9519465919454815 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8172 0.15536916241534027 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7300 0.13879036779637588 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6659 0.12660343276110508 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6399 0.12166021418205607 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5973 0.11356094066407578 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5433 0.103294255922974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011597551281614972 0.0 0.0 2 0.001387903677963759 0.0 0.0 3 0.0015400027111652668 0.0 0.0 4 0.001711114123516963 0.0 0.0 5 0.0020533369482203558 0.0 0.0 6 0.0022814854980226177 0.0 0.0 7 0.002604695943575822 0.0 0.0 8 0.0030609930431803453 0.0 0.0 9 0.003422228247033926 0.0 0.0 10-11 0.0064071717736135176 0.0 0.0 12-13 0.008470014911408967 0.0 1.901237915018848E-5 14-15 0.010475820911753852 0.0 1.901237915018848E-5 16-17 0.011806687452267046 0.0 1.901237915018848E-5 18-19 0.013669900608985517 0.0 1.901237915018848E-5 20-21 0.01542854568037795 0.0 3.802475830037696E-5 22-23 0.017025585528993782 0.0 5.703713745056544E-5 24-25 0.018860280116986972 0.0 7.604951660075392E-5 26-27 0.020504850913478274 0.0 1.1407427490113088E-4 28-29 0.023023991150878248 0.0 1.1407427490113088E-4 30-31 0.0406009356752275 0.0 1.2358046447622512E-4 32-33 0.043623903960107464 0.0 1.6160522277660207E-4 34-35 0.04615255038708253 9.50618957509424E-6 1.7111141235169632E-4 36-37 0.04854811016000628 1.901237915018848E-5 1.7111141235169632E-4 38-39 0.05091515136420475 1.901237915018848E-5 1.7111141235169632E-4 40-41 0.05403318154483566 1.901237915018848E-5 1.7111141235169632E-4 42-43 0.057293804569092985 1.901237915018848E-5 1.7111141235169632E-4 44-45 0.060658995678676345 1.901237915018848E-5 1.9012379150188478E-4 46-47 0.0641952982006114 1.901237915018848E-5 2.0913617065207327E-4 48-49 0.06880580014453211 1.901237915018848E-5 2.4716092895245024E-4 50-51 0.07432889628766186 1.901237915018848E-5 2.4716092895245024E-4 52-53 0.07949075722693802 1.901237915018848E-5 2.4716092895245024E-4 54-55 0.08549866903839759 1.901237915018848E-5 2.4716092895245024E-4 56-57 0.09261880503014318 1.901237915018848E-5 2.4716092895245024E-4 58-59 0.10057548570449706 1.901237915018848E-5 2.4716092895245024E-4 60-61 0.10954932866338601 1.901237915018848E-5 2.4716092895245024E-4 62-63 0.11986354435236327 1.901237915018848E-5 2.4716092895245024E-4 64-65 0.13214554128338502 1.901237915018848E-5 2.4716092895245024E-4 66-67 0.14501692196806262 1.901237915018848E-5 2.4716092895245024E-4 68-69 0.15901953921217643 1.901237915018848E-5 2.4716092895245024E-4 70-71 0.1736400587786714 1.901237915018848E-5 2.5666711852754447E-4 72-73 0.19135008995707195 1.901237915018848E-5 2.851856872528272E-4 74-75 0.21126555711689438 1.901237915018848E-5 3.0419806640301567E-4 76-77 0.23439411635309865 1.901237915018848E-5 3.137042559781099E-4 78-79 0.25967107443327425 1.901237915018848E-5 3.2321044555320413E-4 80-81 0.288798039291363 1.901237915018848E-5 3.2321044555320413E-4 82-83 0.3208529105385808 1.901237915018848E-5 3.2321044555320413E-4 84-85 0.3574612465922687 1.901237915018848E-5 3.2321044555320413E-4 86-87 0.39896527027713014 1.901237915018848E-5 3.2321044555320413E-4 88-89 0.4451368330433629 1.901237915018848E-5 3.2321044555320413E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 51080 0.0 88.587265 1 TCGGAAG 51070 0.0 88.3016 3 ATCGGAA 51220 0.0 88.05228 2 CGGAAGA 51170 0.0 87.98051 4 GAAGAGC 52470 0.0 85.71117 6 GGAAGAG 54745 0.0 82.40089 5 TCGGACG 150 0.0 75.99853 3 AGAGCAC 69660 0.0 64.49205 8 GAGCACA 69790 0.0 64.365105 9 AAGAGCA 71495 0.0 62.83014 7 GTCGCGC 25 0.004990224 57.092262 1 GCCGTCT 48005 0.0 46.364723 50-51 ATGCCGT 47850 0.0 46.348686 48-49 TCGTATG 48295 0.0 46.24473 44-45 CGTCTTC 48195 0.0 46.197163 52-53 TATGCCG 47980 0.0 46.173607 48-49 TGCCGTC 47935 0.0 46.169823 50-51 TCTCGTA 47920 0.0 46.1658 42-43 TATCTCG 47520 0.0 46.118366 40-41 CGTATCT 47425 0.0 46.091225 38-39 >>END_MODULE