FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5259731
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4182517.9519465919454815TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81720.15536916241534027No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC73000.13879036779637588No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG66590.12660343276110508No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA63990.12166021418205607No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG59730.11356094066407578No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA54330.103294255922974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA510800.088.5872651
TCGGAAG510700.088.30163
ATCGGAA512200.088.052282
CGGAAGA511700.087.980514
GAAGAGC524700.085.711176
GGAAGAG547450.082.400895
TCGGACG1500.075.998533
AGAGCAC696600.064.492058
GAGCACA697900.064.3651059
AAGAGCA714950.062.830147
GTCGCGC250.00499022457.0922621
GCCGTCT480050.046.36472350-51
ATGCCGT478500.046.34868648-49
TCGTATG482950.046.2447344-45
CGTCTTC481950.046.19716352-53
TATGCCG479800.046.17360748-49
TGCCGTC479350.046.16982350-51
TCTCGTA479200.046.165842-43
TATCTCG475200.046.11836640-41
CGTATCT474250.046.09122538-39
Produced by FastQC (version 0.11.2)