##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6556135 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.66453985465522 33.0 33.0 33.0 33.0 33.0 2 32.70026456136123 33.0 33.0 33.0 33.0 33.0 3 32.72772555781722 33.0 33.0 33.0 33.0 33.0 4 36.659471014553546 37.0 37.0 37.0 37.0 37.0 5 36.665440385226965 37.0 37.0 37.0 37.0 37.0 6 36.52478190275215 37.0 37.0 37.0 37.0 37.0 7 36.45859748159548 37.0 37.0 37.0 37.0 37.0 8 36.378045601562505 37.0 37.0 37.0 37.0 37.0 9 36.4106251625386 37.0 37.0 37.0 37.0 37.0 10-11 36.36133827323568 37.0 37.0 37.0 37.0 37.0 12-13 36.332369147371125 37.0 37.0 37.0 37.0 37.0 14-15 38.163186618335345 40.0 37.0 40.0 37.0 40.0 16-17 38.13926528358553 40.0 37.0 40.0 37.0 40.0 18-19 38.11814964151897 40.0 37.0 40.0 37.0 40.0 20-21 38.065077747788905 40.0 37.0 40.0 37.0 40.0 22-23 37.93421703488412 40.0 37.0 40.0 33.0 40.0 24-25 37.95388540656957 40.0 37.0 40.0 33.0 40.0 26-27 37.87005537561383 40.0 37.0 40.0 33.0 40.0 28-29 37.72829555828244 40.0 37.0 40.0 33.0 40.0 30-31 37.6951781804371 40.0 37.0 40.0 33.0 40.0 32-33 37.566552397105916 40.0 37.0 40.0 33.0 40.0 34-35 37.47037904192028 40.0 37.0 40.0 33.0 40.0 36-37 37.30640163755017 40.0 37.0 40.0 33.0 40.0 38-39 37.25060313736675 40.0 37.0 40.0 33.0 40.0 40-41 37.19725493755085 40.0 37.0 40.0 33.0 40.0 42-43 37.00355895661087 37.0 37.0 40.0 33.0 40.0 44-45 36.86871655327415 37.0 37.0 40.0 33.0 40.0 46-47 36.83340169169793 37.0 37.0 40.0 33.0 40.0 48-49 36.59871677444104 37.0 37.0 40.0 33.0 40.0 50-51 36.47292162226678 37.0 37.0 40.0 33.0 40.0 52-53 36.33944153071894 37.0 37.0 40.0 33.0 40.0 54-55 36.12142718232617 37.0 37.0 40.0 33.0 40.0 56-57 35.92795396983131 37.0 37.0 40.0 30.0 40.0 58-59 35.711276384638204 37.0 35.0 40.0 27.0 40.0 60-61 35.375421036937155 37.0 33.0 40.0 27.0 40.0 62-63 35.11780462116781 37.0 33.0 37.0 27.0 40.0 64-65 34.85311666095954 37.0 33.0 37.0 27.0 40.0 66-67 34.40261548915634 37.0 33.0 37.0 27.0 40.0 68-69 34.11418587323171 37.0 33.0 37.0 27.0 40.0 70-71 33.75000758831232 37.0 33.0 37.0 27.0 37.0 72-73 33.29696200581593 37.0 33.0 37.0 27.0 37.0 74-75 32.74308918287986 37.0 33.0 37.0 22.0 37.0 76-77 30.091909104983348 33.0 30.0 33.0 22.0 37.0 78-79 32.48812478693621 33.0 33.0 37.0 27.0 37.0 80-81 32.90456023251504 37.0 33.0 37.0 27.0 37.0 82-83 32.85302316074944 37.0 33.0 37.0 27.0 37.0 84-85 32.71078333499844 37.0 33.0 37.0 27.0 37.0 86-87 32.53107562611203 33.0 33.0 37.0 22.0 37.0 88-89 32.43468674760358 33.0 33.0 37.0 22.0 37.0 90-91 32.31529826643289 33.0 33.0 37.0 22.0 37.0 92-93 32.110103666260684 33.0 33.0 37.0 22.0 37.0 94-95 31.983455648793075 33.0 33.0 37.0 22.0 37.0 96-97 31.80940882089829 33.0 33.0 37.0 22.0 37.0 98-99 31.709452901747753 33.0 33.0 37.0 22.0 37.0 100-101 30.078747920840556 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13425691310261811 2306 2 0.11510736063578264 2306 3 0.08587798313372019 2306 4 0.12090137985768479 2306 5 0.10584137430776508 2306 6 0.13544533926997815 2306 7 0.19550292986180295 2306 8 0.24649183521011508 2306 9 0.22270215458573261 2306 10-11 0.20970782187677628 2306 12-13 0.2004017104763065 2306 14-15 0.48434194997741997 2306 16-17 0.4225257146412247 2306 18-19 0.5277829634945448 2306 20-21 0.47249425372551457 2306 22-23 0.47426276569309067 2306 24-25 0.4232936151979203 2306 26-27 0.4802037592826025 2306 28-29 0.5410331582065666 2306 30-31 0.5742912032524288 2306 32-33 0.6547210602602576 2306 34-35 0.6307676329176672 2306 36-37 0.6594268974361626 2306 38-39 0.6450884369389982 2306 40-41 0.5739499655375653 2306 42-43 0.5906250536695197 2306 44-45 0.6457719634290982 2306 46-47 0.6897112482298269 2306 48-49 0.5366641769379186 2306 50-51 0.6033353469966372 2306 52-53 0.5622742796065907 2306 54-55 0.6116656118529846 2306 56-57 0.7080653520782931 2306 58-59 0.6204572308413532 2306 60-61 0.7414958763210038 2306 62-63 0.6652215692040855 2306 64-65 0.6115457220736644 2306 66-67 0.7083279824194477 2306 68-69 0.6499428326371302 2306 70-71 0.5480281561155849 2306 72-73 0.4593717521178462 2306 74-75 0.6016198394302421 2306 76-77 0.08129691431451036 2306 78-79 0.5645207753655939 2306 80-81 0.7269234875953074 2306 82-83 0.7650840153844314 2306 84-85 0.7000798792389915 2306 86-87 0.7061330107697685 2306 88-89 0.7210679966795226 2306 90-91 0.5873029813449762 2306 92-93 0.6173099822222383 2306 94-95 0.6132146010797541 2306 96-97 0.5522254437352494 2306 98-99 0.6271882800914028 2306 100-101 0.391320983422915 2307 1 0.13578077886891293 2307 2 0.10875596368241247 2307 3 0.13514988744324086 2307 4 0.15944506009713422 2307 5 0.12400296019921342 2307 6 0.14676924465055663 2307 7 0.1765768434953472 2307 8 0.18874944536897686 2307 9 0.18794658933038733 2307 10-11 0.23817599657572686 2307 12-13 0.21952261287480468 2307 14-15 0.4162445997322095 2307 16-17 0.45000742971922847 2307 18-19 0.5068006487004482 2307 20-21 0.47559952682559015 2307 22-23 0.4404528078382768 2307 24-25 0.46122973901453435 2307 26-27 0.5044674564681557 2307 28-29 0.5829754130657392 2307 30-31 0.49670485880631077 2307 32-33 0.48451782619491723 2307 34-35 0.49895099749004146 2307 36-37 0.37450448456006313 2307 38-39 0.4254540161693754 2307 40-41 0.4892012777604009 2307 42-43 0.4422290191317373 2307 44-45 0.5273812658143981 2307 46-47 0.6366902471624485 2307 48-49 0.5804353824956507 2307 50-51 0.5553240844383609 2307 52-53 0.464665899720373 2307 54-55 0.5223892050877481 2307 56-57 0.5280940357473085 2307 58-59 0.5257347783509729 2307 60-61 0.4742899389482389 2307 62-63 0.5583128214186317 2307 64-65 0.49985286291341424 2307 66-67 0.44421934027205623 2307 68-69 0.5641865026062121 2307 70-71 0.3968352345995356 2307 72-73 0.32167189260179896 2307 74-75 0.2770969071669853 2307 76-77 0.22037676275322937 2307 78-79 0.49369329553036323 2307 80-81 0.3748892670978563 2307 82-83 0.4352107718923577 2307 84-85 0.5988367521321933 2307 86-87 0.4828994681165639 2307 88-89 0.4160444115143349 2307 90-91 0.24920143125309124 2307 92-93 0.33041717184622854 2307 94-95 0.2507389932023827 2307 96-97 0.26634756573987417 2307 98-99 0.2824750434217833 2307 100-101 0.2309774854824873 2201 1 0.11329046537824894 2201 2 0.11550255453432356 2201 3 0.09813735788051758 2201 4 0.10359247954395556 2201 5 0.11123983099543722 2201 6 0.11496939896377256 2201 7 0.1228171360195418 2201 8 0.14430136673632887 2201 9 0.14019209831242563 2201 10-11 0.10981507136732205 2201 12-13 0.20293331248398516 2201 14-15 0.26467861405650694 2201 16-17 0.28618821940036554 2201 18-19 0.3481895096340395 2201 20-21 0.3336722469315845 2201 22-23 0.32016607038880096 2201 24-25 0.3325801055084412 2201 26-27 0.39019495105880964 2201 28-29 0.3104293287637532 2201 30-31 0.2746869198942221 2201 32-33 0.2456923259349253 2201 34-35 0.35270085072474444 2201 36-37 0.42248310714681736 2201 38-39 0.39722706670696084 2201 40-41 0.3219844509099019 2201 42-43 0.24571008221565904 2201 44-45 0.3865555642762928 2201 46-47 0.3873366434455079 2201 48-49 0.28615755160281964 2201 50-51 0.26988047585719954 2201 52-53 0.2477094764324974 2201 54-55 0.37251353625734396 2201 56-57 0.342721922830286 2201 58-59 0.35777343213583634 2201 60-61 0.3132475279718747 2201 62-63 0.2931813197161546 2201 64-65 0.3151154050560194 2201 66-67 0.32768224639259813 2201 68-69 0.32044939698551644 2201 70-71 0.29947659054310805 2201 72-73 0.20832421820269076 2201 74-75 0.2588659321422426 2201 76-77 -0.004659718905301702 2201 78-79 0.34748696868825135 2201 80-81 0.312369139286254 2201 82-83 0.3023217180672404 2201 84-85 0.4269064530664579 2201 86-87 0.514320869012252 2201 88-89 0.4534263702858681 2201 90-91 0.25359789043556447 2201 92-93 0.3248140922910494 2201 94-95 0.2609670664676145 2201 96-97 0.32956482869295556 2201 98-99 0.30328845860822184 2201 100-101 0.2423205556446817 1101 1 0.0890916297352149 1101 2 0.06928489288102213 1101 3 0.07126173092564159 1101 4 0.06840045628869262 1101 5 0.06447096143278941 1101 6 0.09448987882549886 1101 7 0.06305508567641738 1101 8 0.14410768678305175 1101 9 0.13777070037811256 1101 10-11 0.11799266939431163 1101 12-13 0.1452426470379251 1101 14-15 0.21762360826659943 1101 16-17 0.22053156920959083 1101 18-19 0.2210803508836534 1101 20-21 0.24066567593212085 1101 22-23 0.1492695861183293 1101 24-25 0.21544438265864585 1101 26-27 0.21827797497105195 1101 28-29 0.2661798357361178 1101 30-31 0.2991656862633647 1101 32-33 0.24827301737578011 1101 34-35 0.2501619467870455 1101 36-37 0.21097611542575123 1101 38-39 0.17662793411077615 1101 40-41 0.1209279216410053 1101 42-43 0.16183664255760988 1101 44-45 0.14877442376211292 1101 46-47 0.2948058465111387 1101 48-49 0.3418169117188654 1101 50-51 0.25335932534590455 1101 52-53 0.27337258262171105 1101 54-55 0.3194949869327246 1101 56-57 0.19858543824098973 1101 58-59 0.26482077498579315 1101 60-61 0.17685550881565604 1101 62-63 0.18754757864154215 1101 64-65 0.22002110990416668 1101 66-67 0.2690399758851214 1101 68-69 0.25310467968879635 1101 70-71 0.06297127829253668 1101 72-73 0.04670512894254131 1101 74-75 0.06563750024391624 1101 76-77 -0.059712192395288355 1101 78-79 0.23177555067999833 1101 80-81 0.28597868004501237 1101 82-83 0.21108656145518978 1101 84-85 0.28987190835098176 1101 86-87 0.28893535936981607 1101 88-89 0.38666736120762124 1101 90-91 0.2361195556049438 1101 92-93 0.1927239656419033 1101 94-95 0.15125169971111063 1101 96-97 0.05986384275837864 1101 98-99 0.16175310376172547 1101 100-101 0.21073261913891628 2304 1 0.12141979017376059 2304 2 0.10784651195383788 2304 3 0.07194265015093038 2304 4 0.07221912737876579 2304 5 0.10833561834390082 2304 6 0.07797322461387068 2304 7 0.09273757488887924 2304 8 0.1450971310230429 2304 9 0.09274156921159005 2304 10-11 0.1611018444393011 2304 12-13 0.10414028730613722 2304 14-15 0.2023506545319833 2304 16-17 0.26086850053191313 2304 18-19 0.2625238989125833 2304 20-21 0.31776311864668116 2304 22-23 0.23203918180153948 2304 24-25 0.2420843772867869 2304 26-27 0.24446177667180535 2304 28-29 0.24389225537269255 2304 30-31 0.30147417695701506 2304 32-33 0.2825425572599727 2304 34-35 0.20941516687114614 2304 36-37 0.2965862325687141 2304 38-39 0.2892651595192248 2304 40-41 0.22991241563574505 2304 42-43 0.22587839093257855 2304 44-45 0.3321546898419854 2304 46-47 0.3312968357262065 2304 48-49 0.3820982626314233 2304 50-51 0.41917902380824756 2304 52-53 0.3690845075543834 2304 54-55 0.26933682778503965 2304 56-57 0.38636737765735774 2304 58-59 0.33151961671628527 2304 60-61 0.19699235867834375 2304 62-63 0.30197540045676163 2304 64-65 0.3083852172232113 2304 66-67 0.3464166687148378 2304 68-69 0.25410417977479227 2304 70-71 0.24621866875494902 2304 72-73 0.2852069157315711 2304 74-75 0.09790720196894398 2304 76-77 0.06625172511602528 2304 78-79 0.38429562766842906 2304 80-81 0.39812271357090623 2304 82-83 0.3856791840129077 2304 84-85 0.50551872459544 2304 86-87 0.4750350031123247 2304 88-89 0.5566960141749391 2304 90-91 0.5103047488016088 2304 92-93 0.5100383362729133 2304 94-95 0.47908494070190244 2304 96-97 0.2590103637581045 2304 98-99 0.26677074867457407 2304 100-101 0.2609678366718242 2202 1 0.06010054636964668 2202 2 0.05108382786303167 2202 3 0.03844823208665815 2202 4 0.06985808914073743 2202 5 0.0791838877949047 2202 6 0.05098079844059811 2202 7 0.10294103351633765 2202 8 0.09388324962230143 2202 9 0.077837276195595 2202 10-11 0.027152027683641222 2202 12-13 0.12549857960853927 2202 14-15 0.07859473086746505 2202 16-17 0.09529424559895716 2202 18-19 0.09194180535246232 2202 20-21 0.1296984077275738 2202 22-23 0.12144213763802014 2202 24-25 0.02512000419273619 2202 26-27 0.03113606762065757 2202 28-29 0.1411384562137954 2202 30-31 0.1732343421782474 2202 32-33 0.1761263451404531 2202 34-35 0.06375548444233914 2202 36-37 0.10067502766109726 2202 38-39 0.13137692845703697 2202 40-41 0.06521893219694164 2202 42-43 0.1222918128841215 2202 44-45 0.14157058158201608 2202 46-47 0.25647928431286005 2202 48-49 0.14670758254455052 2202 50-51 0.11741874413043973 2202 52-53 0.1699498983258394 2202 54-55 0.14367508645699445 2202 56-57 0.10143589443948287 2202 58-59 -0.049134683119277156 2202 60-61 8.492224998377651E-4 2202 62-63 0.1402315985697058 2202 64-65 0.09738919454022721 2202 66-67 0.20310380815803342 2202 68-69 0.08836455650981634 2202 70-71 -0.02448986800132502 2202 72-73 0.1101951707372848 2202 74-75 0.007823912205530803 2202 76-77 0.07556011342177982 2202 78-79 0.2767004302141558 2202 80-81 0.15495853443295715 2202 82-83 0.12204630744933098 2202 84-85 0.2905458466841111 2202 86-87 0.2687093205478064 2202 88-89 0.18435738139155688 2202 90-91 0.13735088711049315 2202 92-93 0.25157645669978024 2202 94-95 0.2494014389002288 2202 96-97 0.10061900197833396 2202 98-99 0.050770581640517065 2202 100-101 0.2583411568935574 1102 1 0.057156701417774514 1102 2 0.06591576803590726 1102 3 0.059561304339169396 1102 4 0.04569091536181702 1102 5 0.03689208111295983 1102 6 -0.02461100847325781 1102 7 -0.013091018455469339 1102 8 0.0883056807831224 1102 9 0.059619207411870434 1102 10-11 -0.0035747696291466013 1102 12-13 0.10694091163400543 1102 14-15 -0.007692857899755268 1102 16-17 0.061504475380537826 1102 18-19 0.06710341044789914 1102 20-21 0.045148952666366426 1102 22-23 0.004857084400384792 1102 24-25 -0.030943326446021047 1102 26-27 0.011614115651219947 1102 28-29 0.025722245608200467 1102 30-31 -0.03259177057422136 1102 32-33 0.0237633547910292 1102 34-35 0.053594891242966014 1102 36-37 0.0023781620297498307 1102 38-39 0.022517323719306148 1102 40-41 0.021420346202582152 1102 42-43 -0.0714492041458854 1102 44-45 -0.01850187481403509 1102 46-47 -0.011064622517935163 1102 48-49 -0.05214506731127244 1102 50-51 -0.06711028859668033 1102 52-53 -0.15307817080184094 1102 54-55 -0.1744368415133266 1102 56-57 -0.08699164143867932 1102 58-59 -0.042054456317082156 1102 60-61 -0.15128110021243657 1102 62-63 -0.15290169685093957 1102 64-65 -0.20826728905907999 1102 66-67 -0.18501262032584975 1102 68-69 -0.17024860969548428 1102 70-71 -0.40377135278465204 1102 72-73 -0.36142757831787975 1102 74-75 -0.41154090877744665 1102 76-77 -0.2346145147186398 1102 78-79 -0.16315245689168734 1102 80-81 -0.16692790693942783 1102 82-83 -0.23750703215832658 1102 84-85 -0.1844893237940468 1102 86-87 -0.09266318268633 1102 88-89 -0.13343629458079675 1102 90-91 -0.35175666583089793 1102 92-93 -0.26145335010057735 1102 94-95 -0.258553687512169 1102 96-97 -0.36842626913087173 1102 98-99 -0.4239251712656156 1102 100-101 -0.3171370265128637 2305 1 0.0759026425379119 2305 2 0.013741670308647258 2305 3 0.039899870241860924 2305 4 0.03142151173139496 2305 5 0.050356222035382814 2305 6 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1213 94-95 -0.31541558040954243 1213 96-97 -0.225709861460615 1213 98-99 -0.23518026643629497 1213 100-101 0.06205437243061951 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 8.0 8 141.0 9 293.0 10 224.0 11 111.0 12 103.0 13 218.0 14 534.0 15 1149.0 16 2231.0 17 4010.0 18 5938.0 19 8302.0 20 11632.0 21 15806.0 22 20605.0 23 27482.0 24 34612.0 25 45735.0 26 59863.0 27 82118.0 28 120951.0 29 165991.0 30 159593.0 31 164050.0 32 217026.0 33 308694.0 34 473371.0 35 812899.0 36 1499527.0 37 1942935.0 38 369605.0 39 378.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.466021386982675 25.782352225921617 21.88673734558717 15.86488904150854 2 29.57018121194881 25.059962920226624 31.06883857638685 14.301017291437715 3 35.60065495905743 19.423135734697347 27.41182114157198 17.564388164673243 4 28.737664634265407 19.27961525130829 31.72181324689526 20.260906867531048 5 33.86348889572897 17.943035261982455 30.69100563350979 17.50247020877878 6 28.839552571751497 19.019712071212687 34.7493607132861 17.39137464374971 7 31.7784487354211 21.942165620445582 27.487338195445943 18.792047448687374 8 28.402877610055317 27.873190530701397 25.250746056937512 18.473185802305782 9 31.09415837227269 23.383548386358733 25.944188153538633 19.578105087829947 10-11 25.011573129595412 28.46898363136208 29.094313951741384 17.42512928730113 12-13 23.72239894388996 27.091296930279807 28.542632206322782 20.643671919507455 14-15 23.3399480029011 27.495559502664296 28.617058678626968 20.547433815807636 16-17 24.371233966353653 26.32018712244333 29.25141108290174 20.057167828301278 18-19 22.00356612546874 26.356077170467053 30.980234543675504 20.660122160388706 20-21 23.400959559252517 26.698496904044838 30.515837456062144 19.3847060806405 22-23 21.960870896532896 29.889488710353344 28.684240329737836 19.465400063375924 24-25 21.829248482528197 26.212326317258565 30.71289715663268 21.245528043580556 26-27 21.687274590898447 26.33975657914305 29.174986482127046 22.797982347831457 28-29 22.848587066069108 28.426269928399044 28.6408325646504 20.08431044088145 30-31 20.844182071493528 27.498542570219747 30.198616181482112 21.458659176804613 32-33 20.681067696309626 28.94214344318744 28.95879979115502 21.41798906934791 34-35 22.525446067880686 29.115013283006824 29.120084879458446 19.239455769654036 36-37 22.24872113975021 29.457974052296564 29.42188515524556 18.871419652707665 38-39 21.014941200930927 27.572029298171298 31.98772170062653 19.425307800271245 40-41 21.025242428148744 27.239251964329707 28.689816301514792 23.045689306006757 42-43 21.32018944900037 28.92005390383722 30.19327236241598 19.566484284746434 44-45 20.903634139994637 27.52670304457265 30.43951840102355 21.130144414409166 46-47 22.500094759028745 27.431072435021807 28.98272579131797 21.08610701463148 48-49 21.069102954660448 29.263446787083815 30.035147980135186 19.63230227812056 50-51 22.45586723646906 27.803339771752622 29.477549959525362 20.263243032252955 52-53 20.78564490497781 27.45914558780738 28.36974773251386 23.385461774700943 54-55 22.539503000293532 27.53381089454699 29.837961875249057 20.088724229910422 56-57 21.193336188209464 27.290416418822836 29.480300668190523 22.035946724777176 58-59 21.173198053612314 27.389940551452103 29.603601085720143 21.83326030921544 60-61 22.371918616084706 27.62567847772613 29.521022225280312 20.481380680908856 62-63 20.820343329648995 27.70738422028402 29.526744890920583 21.945527559146402 64-65 22.710309770739233 27.67080759080101 29.41628361453146 20.202599023928293 66-67 21.675686190745008 30.491898448346888 27.702241276719104 20.130174084189008 68-69 21.744456532213192 30.357171059272307 27.741047796685613 20.157324611828887 70-71 21.42763464927228 30.922928807399952 27.783963936209506 19.865472607118264 72-73 21.36009466824386 31.23632472757782 27.44847356458558 19.95510703959274 74-75 21.935246966811565 30.499364206686575 27.257272255569763 20.308116570932096 76-77 22.062036056801603 29.589916991343785 27.749169200141193 20.598877751713417 78-79 21.78785965924987 29.27679762122325 28.45006225495027 20.485280464576615 80-81 21.35828226530176 29.566967804449142 28.264887450497454 20.809862479751644 82-83 20.505646449416783 29.63155553314977 28.548491571377028 21.314306446056424 84-85 20.666392857782977 29.557615605123356 28.512977511443342 21.263014025650325 86-87 20.51495857365809 29.668939401278692 29.02436012162783 20.79174190343539 88-89 20.60277101571236 29.950297833882004 28.867776615854364 20.579154534551268 90-91 20.98697461512164 29.427342553297624 28.397422901141596 21.188259930439138 92-93 20.66432985736319 29.892631223524656 28.031283044710914 21.41175587440124 94-95 20.400549254418177 30.087021228565774 28.023372989416885 21.489056527599164 96-97 20.721686245674885 30.560746294595575 27.655487068486067 21.06208039124347 98-99 20.952680197812782 30.0123432747003 27.641833425978458 21.393143101508464 100-101 21.036691742767385 29.902918279597124 27.271529637058418 21.78886034057707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27.0 1 19.5 2 24.0 3 66.5 4 90.0 5 68.0 6 56.0 7 60.0 8 56.5 9 59.0 10 62.5 11 50.0 12 55.0 13 71.5 14 77.5 15 91.5 16 107.5 17 125.0 18 170.5 19 245.5 20 334.0 21 483.0 22 733.5 23 1055.0 24 1527.0 25 2312.0 26 3681.5 27 5740.5 28 7967.5 29 10338.0 30 13196.5 31 17340.0 32 28807.5 33 51526.0 34 88495.5 35 141533.0 36 216119.0 37 311630.0 38 400111.5 39 435758.5 40 444797.5 41 434170.5 42 411873.0 43 421593.5 44 420055.0 45 405102.5 46 402940.5 47 364668.5 48 292217.5 49 240390.0 50 200361.0 51 170108.0 52 148375.0 53 120445.0 54 87966.0 55 60138.0 56 42938.5 57 32209.0 58 25875.5 59 20654.5 60 15910.5 61 12516.5 62 9790.0 63 7414.0 64 5819.5 65 4637.5 66 3402.0 67 2474.0 68 1901.0 69 1409.5 70 1012.0 71 758.5 72 597.5 73 420.0 74 273.5 75 187.5 76 138.5 77 108.5 78 73.5 79 45.0 80 28.5 81 18.0 82 13.5 83 11.5 84 12.5 85 9.0 86 4.5 87 3.0 88 3.5 89 2.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14830384060120785 2 0.0 3 0.0 4 0.015237636198766499 5 1.3727600179068918E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.669255590374512E-4 24-25 0.0 26-27 0.0 28-29 1.1439666815890764E-4 30-31 0.0011134609034133677 32-33 0.001159219570676931 34-35 2.1354044722996096E-4 36-37 0.0015557946869611439 38-39 0.007687456100278594 40-41 0.016778177996639786 42-43 0.0058952416324557074 44-45 0.0021506573613874636 46-47 4.8046600626741213E-4 48-49 0.006467224973250245 50-51 0.002715014257638075 52-53 0.01056262569333914 54-55 0.02200991895377383 56-57 0.01934066336339932 58-59 0.018654283354445873 60-61 0.02397754164610704 62-63 0.006909558756798022 64-65 0.010280447245213833 66-67 0.027989051476212737 68-69 0.040854863421817884 70-71 0.03852117139137617 72-73 0.028644925706990475 74-75 0.03830763094414621 76-77 0.031413325076436044 78-79 0.012705656610182674 80-81 0.010127918354335292 82-83 0.004415711390933835 84-85 0.00713072564857191 86-87 0.02363435164163032 88-89 0.02120914227666148 90-91 0.015435923756908606 92-93 0.03113877307285466 94-95 0.03293861398522147 96-97 0.034502035116726545 98-99 0.02369536319798174 100-101 0.023702989642525665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6556135.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.03932821657875 #Duplication Level Percentage of deduplicated Percentage of total 1 85.23619207428138 26.456741417249674 2 6.271531139622855 3.893282269264959 3 1.9473986752941115 1.8133783995295374 4 1.3697591043653217 1.7006560967216866 5 0.7890318330124827 1.2245509019101601 6 0.596674052793778 1.1112217057789389 7 0.4113647362427446 0.8937939545475427 8 0.32251456315903776 0.8008508304415912 9 0.26826279335231723 0.7494027202042932 >10 2.182902606053173 13.695936275433858 >50 0.2740672874385768 5.9097844830519035 >100 0.26028500506322216 17.806962426976945 >500 0.049029615450881525 10.569810902952723 >1k 0.020689569851048282 10.20866915339338 >5k 2.474619872807087E-4 0.4464963233423738 >10k+ 4.9482031781496005E-5 2.718462139200581 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 176996 2.699700356993869 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0014185186851704548 0.0 0.0 2 0.0015405417978732897 0.0 0.0 3 0.0018608524687182312 0.0 0.0 4 0.002074392915948192 0.0 0.0 5 0.0025319795885838225 0.0 0.0 6 0.0029133018157801813 0.0 0.0 7 0.0036149347138214817 1.5252889087854353E-5 0.0 8 0.004118280053720676 1.5252889087854353E-5 0.0 9 0.004713142728146995 1.5252889087854353E-5 0.0 10-11 0.007092593425852275 1.5252889087854353E-5 0.0 12-13 0.009548308568996825 1.5252889087854353E-5 0.0 14-15 0.012133673269388138 1.5252889087854353E-5 0.0 16-17 0.014536003300725199 1.5252889087854353E-5 0.0 18-19 0.017266270447451125 1.5252889087854353E-5 0.0 20-21 0.020751555604025848 1.5252889087854353E-5 0.0 22-23 0.02433598453967162 1.5252889087854353E-5 1.5252889087854353E-5 24-25 0.028286482813425898 1.5252889087854353E-5 2.287933363178153E-5 26-27 0.03274032642707937 1.5252889087854353E-5 3.0505778175708706E-5 28-29 0.037430589821594584 1.5252889087854353E-5 4.575866726356306E-5 30-31 0.0641765308371472 1.5252889087854353E-5 6.863800089534459E-5 32-33 0.07114710115029663 1.5252889087854353E-5 7.626444543927177E-5 34-35 0.0768898138918738 1.5252889087854353E-5 7.626444543927177E-5 36-37 0.0830672339724548 1.5252889087854353E-5 7.626444543927177E-5 38-39 0.08989290183926962 1.5252889087854353E-5 7.626444543927177E-5 40-41 0.09778627194223426 1.5252889087854353E-5 7.626444543927177E-5 42-43 0.10625162538599342 2.287933363178153E-5 7.626444543927177E-5 44-45 0.11648631396394368 3.0505778175708706E-5 7.626444543927177E-5 46-47 0.1283835674524701 3.0505778175708706E-5 7.626444543927177E-5 48-49 0.14082229850361533 3.0505778175708706E-5 7.626444543927177E-5 50-51 0.15406943267641682 3.0505778175708706E-5 9.151733452712612E-5 52-53 0.1683461368626485 3.0505778175708706E-5 9.151733452712612E-5 54-55 0.1840261068449628 3.0505778175708706E-5 9.151733452712612E-5 56-57 0.202642257976689 3.0505778175708706E-5 9.151733452712612E-5 58-59 0.2230048649089746 3.0505778175708706E-5 9.151733452712612E-5 60-61 0.24478599052643057 3.0505778175708706E-5 9.151733452712612E-5 62-63 0.26924399817880507 3.0505778175708706E-5 9.151733452712612E-5 64-65 0.2968669803169093 3.0505778175708706E-5 9.151733452712612E-5 66-67 0.3272812411580909 3.0505778175708706E-5 9.151733452712612E-5 68-69 0.3604715278132619 3.0505778175708706E-5 9.151733452712612E-5 70-71 0.39695643851140955 3.0505778175708706E-5 9.151733452712612E-5 72-73 0.43714017481336187 3.0505778175708706E-5 9.91437790710533E-5 74-75 0.4815565878371937 3.0505778175708706E-5 1.2202311270283482E-4 76-77 0.5331799909550368 3.0505778175708706E-5 1.3727600179068918E-4 78-79 0.5896004276910101 3.0505778175708706E-5 1.3727600179068918E-4 80-81 0.6521296465066689 3.0505778175708706E-5 1.3727600179068918E-4 82-83 0.7235207938823712 3.0505778175708706E-5 1.3727600179068918E-4 84-85 0.8010817348941106 3.8132222719635886E-5 1.3727600179068918E-4 86-87 0.8870470177932577 4.575866726356306E-5 1.3727600179068918E-4 88-89 0.9812717401334781 4.575866726356306E-5 1.3727600179068918E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 28455 0.0 66.40209 4 TCGGAAG 28595 0.0 66.22648 3 GAAGAGC 28860 0.0 65.45876 6 GATCGGA 29585 0.0 64.27864 1 ATCGGAA 30040 0.0 63.13569 2 GGAAGAG 31845 0.0 59.54666 5 TCGCCGG 250 0.0 51.297867 2 TTGTGTT 60855 0.0 45.592266 1 GCCGTCT 21140 0.0 44.503258 50-51 ATGCCGT 20945 0.0 44.472782 48-49 TCGTATG 21200 0.0 44.25649 44-45 TGCCGTC 21100 0.0 44.2049 50-51 CGTCTTC 21260 0.0 44.19654 52-53 TATGCCG 21050 0.0 44.05914 48-49 CGTATGC 21155 0.0 43.969646 46-47 TCTCGTA 20995 0.0 43.930637 42-43 CTCGTAT 20985 0.0 43.736736 44-45 CATCTCG 21175 0.0 43.381573 40-41 ATCTCGT 21130 0.0 43.2229 42-43 GTATGCC 21710 0.0 43.151867 46-47 >>END_MODULE