FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556135
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1769962.699700356993869TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA284550.066.402094
TCGGAAG285950.066.226483
GAAGAGC288600.065.458766
GATCGGA295850.064.278641
ATCGGAA300400.063.135692
GGAAGAG318450.059.546665
TCGCCGG2500.051.2978672
TTGTGTT608550.045.5922661
GCCGTCT211400.044.50325850-51
ATGCCGT209450.044.47278248-49
TCGTATG212000.044.2564944-45
TGCCGTC211000.044.204950-51
CGTCTTC212600.044.1965452-53
TATGCCG210500.044.0591448-49
CGTATGC211550.043.96964646-47
TCTCGTA209950.043.93063742-43
CTCGTAT209850.043.73673644-45
CATCTCG211750.043.38157340-41
ATCTCGT211300.043.222942-43
GTATGCC217100.043.15186746-47
Produced by FastQC (version 0.11.2)