##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3167044 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68124471905032 33.0 33.0 33.0 33.0 33.0 2 32.711265141879935 33.0 33.0 33.0 33.0 33.0 3 32.74226565844996 33.0 33.0 33.0 33.0 33.0 4 36.669860601873545 37.0 37.0 37.0 37.0 37.0 5 36.67409546567714 37.0 37.0 37.0 37.0 37.0 6 36.537751606861164 37.0 37.0 37.0 37.0 37.0 7 36.468216734595416 37.0 37.0 37.0 37.0 37.0 8 36.393116104481024 37.0 37.0 37.0 37.0 37.0 9 36.425345211496904 37.0 37.0 37.0 37.0 37.0 10-11 36.363847328928806 37.0 37.0 37.0 37.0 37.0 12-13 36.332361501766314 37.0 37.0 37.0 37.0 37.0 14-15 38.154884175906616 40.0 37.0 40.0 37.0 40.0 16-17 38.141867463792735 40.0 37.0 40.0 37.0 40.0 18-19 38.10594958579672 40.0 37.0 40.0 37.0 40.0 20-21 38.05924562462663 40.0 37.0 40.0 33.0 40.0 22-23 37.90827614014835 40.0 37.0 40.0 33.0 40.0 24-25 37.9336646412238 40.0 37.0 40.0 33.0 40.0 26-27 37.85952626487033 40.0 37.0 40.0 33.0 40.0 28-29 37.6746222976378 40.0 37.0 40.0 33.0 40.0 30-31 37.62850089862977 40.0 37.0 40.0 33.0 40.0 32-33 37.504745908171785 40.0 37.0 40.0 33.0 40.0 34-35 37.471370779818656 40.0 37.0 40.0 33.0 40.0 36-37 37.37042743959351 40.0 37.0 40.0 33.0 40.0 38-39 37.16518052796235 40.0 37.0 40.0 33.0 40.0 40-41 37.04851921855206 40.0 37.0 40.0 33.0 40.0 42-43 36.89858255837305 37.0 37.0 40.0 33.0 40.0 44-45 36.709375367061526 37.0 37.0 40.0 33.0 40.0 46-47 36.724115926396976 37.0 37.0 40.0 33.0 40.0 48-49 36.46913194133078 37.0 37.0 40.0 33.0 40.0 50-51 36.40266791367597 37.0 37.0 40.0 33.0 40.0 52-53 36.33250059045596 37.0 37.0 40.0 33.0 40.0 54-55 36.03515928417792 37.0 37.0 40.0 30.0 40.0 56-57 35.844900323456194 37.0 37.0 40.0 27.0 40.0 58-59 35.691802671513244 37.0 37.0 40.0 27.0 40.0 60-61 35.427782026394325 37.0 33.0 40.0 27.0 40.0 62-63 35.212343118693646 37.0 33.0 40.0 27.0 40.0 64-65 35.010202415880556 37.0 33.0 40.0 27.0 40.0 66-67 34.519764960638376 37.0 33.0 38.5 27.0 40.0 68-69 34.31229184059331 37.0 33.0 37.0 27.0 40.0 70-71 34.013396245836816 37.0 33.0 37.0 27.0 40.0 72-73 33.55155927735768 37.0 33.0 37.0 24.5 40.0 74-75 32.906166444166864 37.0 33.0 37.0 22.0 40.0 76-77 30.328284829639244 33.0 30.0 35.0 22.0 37.0 78-79 32.495058009929764 35.0 33.0 37.0 22.0 37.0 80-81 32.757766706114595 37.0 33.0 37.0 22.0 37.0 82-83 32.61977904317085 37.0 33.0 37.0 22.0 37.0 84-85 32.415190632021535 37.0 33.0 37.0 22.0 37.0 86-87 32.189356699812194 37.0 33.0 37.0 22.0 37.0 88-89 32.0298431281662 35.0 33.0 37.0 22.0 37.0 90-91 31.866371607088503 33.0 33.0 37.0 18.5 37.0 92-93 31.649113179355894 33.0 33.0 37.0 15.0 37.0 94-95 31.50371782015027 33.0 33.0 37.0 15.0 37.0 96-97 31.323977342910297 33.0 33.0 37.0 15.0 37.0 98-99 31.220493463305218 33.0 33.0 37.0 15.0 37.0 100-101 29.621259919344347 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18078038720963718 2306 2 0.17996924867455988 2306 3 0.14316696952129604 2306 4 0.18328444802533994 2306 5 0.15481777558191112 2306 6 0.185816224773923 2306 7 0.2468026478722436 2306 8 0.2950670948071803 2306 9 0.2231435158456918 2306 10-11 0.22993526175918788 2306 12-13 0.22280612774639508 2306 14-15 0.47311350761987114 2306 16-17 0.4718658018632951 2306 18-19 0.49286322876439215 2306 20-21 0.4871633841940479 2306 22-23 0.5196736253870498 2306 24-25 0.3806707122298292 2306 26-27 0.4199259492582925 2306 28-29 0.43226280372194736 2306 30-31 0.49754059302398446 2306 32-33 0.5275910091533049 2306 34-35 0.5084623178159049 2306 36-37 0.47113164291030785 2306 38-39 0.5798564300572195 2306 40-41 0.475364514288664 2306 42-43 0.47480374352507937 2306 44-45 0.649563064165882 2306 46-47 0.5975692564174437 2306 48-49 0.4821557193474675 2306 50-51 0.47816857944742 2306 52-53 0.6237085386206758 2306 54-55 0.5124282662059798 2306 56-57 0.5985810910820177 2306 58-59 0.3850435076496268 2306 60-61 0.3366267985306166 2306 62-63 0.510203219843639 2306 64-65 0.4698316494345889 2306 66-67 0.4717455807900208 2306 68-69 0.6455346284363443 2306 70-71 0.32722493836178757 2306 72-73 0.343157384760417 2306 74-75 0.33200937119609364 2306 76-77 -0.28915552788827625 2306 78-79 0.5200171852410875 2306 80-81 0.5748274661298396 2306 82-83 0.4311878290006774 2306 84-85 0.4960118450000124 2306 86-87 0.4354468826439799 2306 88-89 0.5509194112952756 2306 90-91 0.4258884973186241 2306 92-93 0.2955723109866142 2306 94-95 0.3229587303892174 2306 96-97 0.2965876001885057 2306 98-99 0.3853968461226529 2306 100-101 0.28380831418672514 2307 1 0.13959954284678133 2307 2 0.13018919703758058 2307 3 0.12866501724280255 2307 4 0.14910304054843948 2307 5 0.16901184509855938 2307 6 0.1485919467895016 2307 7 0.24830770979873762 2307 8 0.19332677064097936 2307 9 0.1971073571181634 2307 10-11 0.1631054397019298 2307 12-13 0.2534444756198866 2307 14-15 0.45360629340345326 2307 16-17 0.38558831955451467 2307 18-19 0.5302287668272498 2307 20-21 0.5067885826805139 2307 22-23 0.4860678527297324 2307 24-25 0.4033179132576876 2307 26-27 0.33449987996765884 2307 28-29 0.5061809923054383 2307 30-31 0.4665211639843818 2307 32-33 0.549145370847171 2307 34-35 0.4591169619657123 2307 36-37 0.49326950504175926 2307 38-39 0.42635151067499066 2307 40-41 0.5126287624543693 2307 42-43 0.3858916002308561 2307 44-45 0.5713488034472931 2307 46-47 0.5692138640386872 2307 48-49 0.40673744504479004 2307 50-51 0.21735961508284163 2307 52-53 0.2820278583121407 2307 54-55 0.38519242415785726 2307 56-57 0.39732432584791866 2307 58-59 0.4013673938941693 2307 60-61 0.3769183464906618 2307 62-63 0.4642099014453507 2307 64-65 0.30754147683403943 2307 66-67 0.364786178294473 2307 68-69 0.30575887238993715 2307 70-71 0.22240915755633495 2307 72-73 0.27532092412157994 2307 74-75 0.1427563416489761 2307 76-77 0.1898307512634716 2307 78-79 0.6470228375704963 2307 80-81 0.6312013134957581 2307 82-83 0.6153757215993565 2307 84-85 0.4995211948119689 2307 86-87 0.47388326841497985 2307 88-89 0.49838848015378545 2307 90-91 0.43360161864626434 2307 92-93 0.4558695928216636 2307 94-95 0.39699105069515994 2307 96-97 0.6256922809696199 2307 98-99 0.3841735017389283 2307 100-101 0.350821292777475 2201 1 0.0835870834014969 2201 2 0.10207881481409231 2201 3 0.10359070740152987 2201 4 0.10393323419654621 2201 5 0.10813659712926693 2201 6 0.1765965575613535 2201 7 0.10977355101636022 2201 8 0.18301351445605007 2201 9 0.14616922910016683 2201 10-11 0.16474273860126942 2201 12-13 0.21266796206279537 2201 14-15 0.2291673677823738 2201 16-17 0.3324281815767378 2201 18-19 0.39019141223600684 2201 20-21 0.37781459814040375 2201 22-23 0.3944975676001192 2201 24-25 0.3828624700213439 2201 26-27 0.42080182940026845 2201 28-29 0.461430494208237 2201 30-31 0.31966667744001853 2201 32-33 0.4252257506746062 2201 34-35 0.4576874284178132 2201 36-37 0.532158591802407 2201 38-39 0.4357403459586138 2201 40-41 0.4599740069483218 2201 42-43 0.4005517392879412 2201 44-45 0.4776781994923027 2201 46-47 0.5894407653884599 2201 48-49 0.5787204762819087 2201 50-51 0.49646888994399063 2201 52-53 0.3589884616843051 2201 54-55 0.4568930564121061 2201 56-57 0.5411014567649204 2201 58-59 0.47481784212767764 2201 60-61 0.42477561115457974 2201 62-63 0.4437558073346608 2201 64-65 0.5941165432649882 2201 66-67 0.5398430574322433 2201 68-69 0.40241293282644364 2201 70-71 0.3755190001295645 2201 72-73 0.3365596265433126 2201 74-75 0.18839618338034825 2201 76-77 -0.07019622045669749 2201 78-79 0.4306746185243 2201 80-81 0.3666307563824134 2201 82-83 0.23222592332061964 2201 84-85 0.3167678236167788 2201 86-87 0.4178578573633729 2201 88-89 0.2769305825880437 2201 90-91 0.1499287913321865 2201 92-93 0.2206489945869663 2201 94-95 0.30868996686157146 2201 96-97 0.21544259558572776 2201 98-99 0.14900282115360142 2201 100-101 0.07223197094516465 1101 1 0.06398039421193857 1101 2 0.08075624146601967 1101 3 0.07219811935901532 1101 4 0.1316025559165439 1101 5 0.08963566930123079 1101 6 0.08620550884899814 1101 7 0.08205687236486625 1101 8 0.15022918435892763 1101 9 0.06257504949693526 1101 10-11 0.05688341903300653 1101 12-13 0.14261694531407443 1101 14-15 0.24159465420260773 1101 16-17 0.3262706310427532 1101 18-19 0.2867677819547936 1101 20-21 0.2631862259130884 1101 22-23 0.24550461504716026 1101 24-25 0.2467770399560365 1101 26-27 0.2792124204331614 1101 28-29 0.23986396369658536 1101 30-31 0.3063731729719237 1101 32-33 0.2777615534343667 1101 34-35 0.372948406490174 1101 36-37 0.270853848568926 1101 38-39 0.20757235115224404 1101 40-41 0.21994976755581064 1101 42-43 0.22864010088523656 1101 44-45 0.34500913524687604 1101 46-47 0.37570537017417394 1101 48-49 0.41134797677386814 1101 50-51 0.38028532528201 1101 52-53 0.3748893560406472 1101 54-55 0.30568743169602186 1101 56-57 0.17197325649382833 1101 58-59 0.09542882010808285 1101 60-61 -0.019562522853583175 1101 62-63 0.13619345710670672 1101 64-65 -0.026154200450335452 1101 66-67 -0.05389309181473578 1101 68-69 -0.01749913960482985 1101 70-71 0.01758312785166538 1101 72-73 -0.23143228323760212 1101 74-75 -0.22961195649455135 1101 76-77 -0.16290844847618757 1101 78-79 0.10109957112138801 1101 80-81 0.044704313191424205 1101 82-83 -0.012099290980984279 1101 84-85 0.044036266612828 1101 86-87 0.1392492132061136 1101 88-89 0.11504665948466908 1101 90-91 -0.004336872777511047 1101 92-93 -0.11537280247524606 1101 94-95 -0.14439701540762684 1101 96-97 -0.12818527640526867 1101 98-99 -0.14382968427635134 1101 100-101 -0.2031373211652614 2304 1 0.06788969972628678 2304 2 0.10796910076300748 2304 3 0.1032521419208905 2304 4 0.1412055736322202 2304 5 0.11672999175911514 2304 6 0.10686619506555672 2304 7 0.1587221615794192 2304 8 0.19297058868523465 2304 9 0.11969731685031348 2304 10-11 0.14323794453289196 2304 12-13 0.06263613031524073 2304 14-15 0.17826388295943474 2304 16-17 0.2196918802939507 2304 18-19 0.20252336556576722 2304 20-21 0.34858413063751925 2304 22-23 0.31071214654050294 2304 24-25 0.23013247387316937 2304 26-27 0.2515897216582772 2304 28-29 0.35612603484410243 2304 30-31 0.24373642287079633 2304 32-33 0.43105729386454783 2304 34-35 0.3711806519284764 2304 36-37 0.4413812520512437 2304 38-39 0.3188111772475324 2304 40-41 0.37509225058926887 2304 42-43 0.4089723195486741 2304 44-45 0.4292328460327681 2304 46-47 0.22287358862902806 2304 48-49 0.41382366576650753 2304 50-51 0.4202137255254783 2304 52-53 0.38182091273829144 2304 54-55 0.396955429685498 2304 56-57 0.5702078463760927 2304 58-59 0.46672794004325624 2304 60-61 0.3122673353710965 2304 62-63 0.34385203244727336 2304 64-65 0.37534026206068916 2304 66-67 0.3445356267910782 2304 68-69 0.42171140066460566 2304 70-71 0.2494811721471919 2304 72-73 0.2590896995397216 2304 74-75 0.14727625209172146 2304 76-77 0.1838052326908297 2304 78-79 0.5415996651182553 2304 80-81 0.45523649758653306 2304 82-83 0.5239348125715679 2304 84-85 0.6374186462979381 2304 86-87 0.4621296496320326 2304 88-89 0.5160279736090487 2304 90-91 0.5071458631316119 2304 92-93 0.5453547157314418 2304 94-95 0.6215848618215105 2304 96-97 0.2883135178493994 2304 98-99 0.3271418270048052 2304 100-101 0.5334825866257908 2202 1 0.10196995821659272 2202 2 0.10109098448044307 2202 3 0.09069228831790355 2202 4 0.09204979429750892 2202 5 0.12082887886607807 2202 6 0.08296560042473544 2202 7 0.07595946324490654 2202 8 0.11284905447384119 2202 9 0.08012014708038606 2202 10-11 0.0435560855969328 2202 12-13 0.1298610578772923 2202 14-15 0.07299011215887674 2202 16-17 0.07607428389377446 2202 18-19 0.1492208291795265 2202 20-21 0.07398725009446139 2202 22-23 0.09602145199566081 2202 24-25 0.02240232802508757 2202 26-27 -0.04849088097812171 2202 28-29 0.06899249636618521 2202 30-31 0.13508338515315899 2202 32-33 0.005917662411370372 2202 34-35 -0.03598242914380023 2202 36-37 -0.003130551216962374 2202 38-39 0.07517626264732513 2202 40-41 0.12552673040469386 2202 42-43 0.1693674443552382 2202 44-45 0.13205558325837785 2202 46-47 0.30893912831274406 2202 48-49 0.171010764982789 2202 50-51 0.07800988199402781 2202 52-53 0.15289048070556532 2202 54-55 0.23492657205644463 2202 56-57 0.12023424877601485 2202 58-59 0.08121990962902714 2202 60-61 -0.037319938838471955 2202 62-63 0.06910932415802762 2202 64-65 0.08932797075932797 2202 66-67 0.15472584420749058 2202 68-69 0.2898799760605968 2202 70-71 -0.07323221090310739 2202 72-73 0.038684276226170766 2202 74-75 -0.13207777350261551 2202 76-77 -0.13663673073098437 2202 78-79 0.014167322076687583 2202 80-81 0.06233829034368199 2202 82-83 0.14744287366692532 2202 84-85 0.13936181487795096 2202 86-87 0.06300705581294608 2202 88-89 0.1323586850617673 2202 90-91 -0.1548633105240924 2202 92-93 0.021716758894605448 2202 94-95 0.07673183271677075 2202 96-97 0.0950112623987387 2202 98-99 -0.18016933669136748 2202 100-101 0.10798051130658948 1102 1 0.10242190373886473 1102 2 0.06523711419692546 1102 3 0.0765305219980803 1102 4 0.07508625042967765 1102 5 0.09060461282204813 1102 6 -0.028579536548924978 1102 7 0.05842440401583815 1102 8 0.011296824446525022 1102 9 0.06191136874678449 1102 10-11 0.00563935090119827 1102 12-13 0.049768126490306486 1102 14-15 -0.06157051067471997 1102 16-17 0.04187752346774687 1102 18-19 0.05068204378419239 1102 20-21 -0.003753073538383944 1102 22-23 0.056961401887356544 1102 24-25 -0.014701616455788269 1102 26-27 0.04583093241806324 1102 28-29 -0.020331795968978383 1102 30-31 -0.1179105226211945 1102 32-33 0.01091582402509772 1102 34-35 0.07604568457224303 1102 36-37 -0.05185547411220881 1102 38-39 -0.04269604046562847 1102 40-41 0.10134522509728328 1102 42-43 0.043875126219184324 1102 44-45 0.0649980418391678 1102 46-47 0.013765394048455448 1102 48-49 0.09137775044067808 1102 50-51 0.06240544862909303 1102 52-53 0.013233549348761642 1102 54-55 -0.012072972297069384 1102 56-57 0.06237540011684217 1102 58-59 0.06858586151953716 1102 60-61 0.20362531482964386 1102 62-63 -0.018406189672710127 1102 64-65 -0.1400473351935574 1102 66-67 -0.02081207416417641 1102 68-69 0.003167943078686619 1102 70-71 -0.17798948199484244 1102 72-73 -0.027662465203910358 1102 74-75 -0.1453452570685272 1102 76-77 0.03966622109247453 1102 78-79 0.029649267529215706 1102 80-81 -0.06951450267252568 1102 82-83 -0.10712949832679897 1102 84-85 0.1238025760652377 1102 86-87 0.20836490627748105 1102 88-89 0.18494364975236977 1102 90-91 -5.6690287179606E-4 1102 92-93 -0.04865364996164345 1102 94-95 -0.1867094897370336 1102 96-97 -0.17629327158991615 1102 98-99 -0.07864578919089737 1102 100-101 -0.1616838518850905 2305 1 -0.01667389369126937 2305 2 3.8519645551104986E-4 2305 3 -0.04125226227183987 2305 4 -0.028531086409032014 2305 5 -0.03735505775244263 2305 6 -0.10589611526765452 2305 7 -0.03947562745234734 2305 8 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92-93 -0.07070777858418609 1213 94-95 -0.38768710063241585 1213 96-97 -0.25561307947821277 1213 98-99 -0.3516844067766165 1213 100-101 -0.06017661892634507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 15.0 10 24.0 11 27.0 12 36.0 13 136.0 14 328.0 15 825.0 16 1589.0 17 2698.0 18 4099.0 19 5775.0 20 7834.0 21 10221.0 22 13336.0 23 17220.0 24 21832.0 25 27951.0 26 36308.0 27 50140.0 28 73654.0 29 88581.0 30 75758.0 31 79280.0 32 103956.0 33 144208.0 34 211241.0 35 335050.0 36 572174.0 37 894138.0 38 387724.0 39 882.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.03418249362268 26.340074987849338 19.53292125959602 16.092821258931963 2 29.560025058066763 27.702962131249205 28.428623031445095 14.308389779238937 3 34.81265179770158 20.98174196506269 27.5574005286949 16.648205708540832 4 29.39428591639989 21.644028299774483 28.232255717383747 20.72943006644188 5 34.37516873016089 19.866790399486714 28.791498936388216 16.966541933964187 6 31.112103273588875 20.704606566880663 30.99975876558709 17.183531393943376 7 31.12318616350136 24.87290988063317 25.103219279555322 18.90068467631015 8 28.659153456661794 30.260331084759162 23.014552371233236 18.0659630873458 9 31.844647564100782 24.46666355124842 24.81689550255696 18.871793382093838 10-11 26.411221315523242 30.00528252843977 27.92019940360791 15.66329675242908 12-13 24.020032560330705 29.349892202318628 27.678554513293786 18.95152072405688 14-15 23.314642928863634 29.67167491199996 27.91866800713852 19.095014151997887 16-17 24.963514873806616 27.603926563697883 28.060582675832734 19.371975886662767 18-19 22.066823195383456 27.7050303058625 30.552401545415854 19.675744953338192 20-21 24.008997033195623 27.750293333468058 30.458102255604913 17.78260737773141 22-23 21.591099439491774 32.528911491863134 27.973343534247235 17.90664553439785 24-25 21.32441165957909 27.90426972280777 30.451739855840337 20.319578761772807 26-27 21.301124960688895 27.863963999237146 28.176400454177458 22.6585105858965 28-29 23.357516267947947 30.347559496002656 28.034358891210896 18.2605653448385 30-31 20.877160077498818 28.16083025788313 30.388416098939825 20.573593565678227 32-33 20.836079749125872 30.428197731832885 28.10327354869654 20.632448970344708 34-35 20.710537844910267 28.170253746600725 30.46978445945146 20.649423949037544 36-37 22.992816718247973 28.09301149208251 28.176276240670806 20.737895548998715 38-39 22.921195075112568 30.475255331581728 28.09226056412858 18.51128902917712 40-41 22.75821711519641 30.41846108564862 28.11199385068392 18.711327948471055 42-43 20.497804453993318 30.331927527088293 30.321901842389288 18.8483661765291 44-45 20.3427210419523 28.104205333917697 30.53717139809623 21.015902226033777 46-47 22.660124264591538 28.050359445316715 28.104653172962387 21.184863117129364 48-49 20.26141561865642 30.481321332074664 30.230471469222657 19.026791580046257 50-51 22.558107004227086 28.062946189072864 30.21537380180798 19.163573004892076 52-53 20.289823722857545 28.15800143871352 27.944247009048485 23.60792782938045 54-55 22.32356061254441 28.112455189633394 30.432693416085595 19.131290781736602 56-57 20.11185322714151 28.111152762807556 30.323710528218957 21.453283481831985 58-59 20.05385135736875 28.389616402241725 30.279340494565915 21.277191745823615 60-61 22.18373719191469 28.241939632894496 30.309503758396406 19.264819416794403 62-63 19.966411402376842 28.362092471427037 30.218275361135845 21.45322076506028 64-65 22.1331611689808 28.292441931240674 30.288910541008796 19.28548635876973 66-67 20.04696168717181 32.52860370703285 27.87005108334981 19.55438352244553 68-69 19.99144609078768 32.71653898977953 27.834430053155536 19.457584866277255 70-71 19.893852410471037 32.66479251391795 27.92378094523631 19.517574130374697 72-73 19.8250139086528 32.60818310435445 27.891006064219997 19.675796922772747 74-75 19.755367098599397 32.30540640038738 28.076448729289567 19.862777771723657 76-77 19.89909010856286 31.90744299281259 28.067519832020043 20.125947066604507 78-79 19.89622556901938 31.728743325809145 28.177574845042564 20.197456260128916 80-81 19.885737545292965 31.424338149162335 28.206269877471957 20.483654428072743 82-83 19.93765870104453 31.39075281783903 28.05614221887091 20.615446262245527 84-85 19.91472237405635 31.182241059127524 28.260520842115767 20.642515724700356 86-87 20.090644316515135 30.829063408385476 28.29235847662216 20.78793379847723 88-89 20.12220146440267 31.002960607918652 28.15647244727113 20.718365480407545 90-91 20.157323635003987 30.76359501148474 28.180566908888053 20.89851444462322 92-93 20.148289342670772 30.772802250081526 28.096013796120317 20.982894611127385 94-95 20.065725108972003 30.690979071658496 28.21225587404935 21.031039945320146 96-97 20.143672429261773 30.628282214760706 28.113027659477357 21.115017696500164 98-99 20.13846209236238 30.6746813527483 28.069966276670264 21.11689027821905 100-101 20.26361068439989 30.56654442232284 27.91565708756114 21.254187805716136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 6.0 2 7.5 3 15.0 4 19.5 5 13.5 6 10.5 7 13.5 8 16.5 9 23.0 10 28.0 11 30.5 12 40.5 13 59.0 14 85.5 15 129.5 16 224.5 17 407.0 18 647.5 19 1072.5 20 1844.0 21 2971.5 22 4548.0 23 6863.5 24 9902.5 25 13703.0 26 18609.0 27 24511.0 28 31360.0 29 39119.0 30 47255.0 31 56082.0 32 66322.5 33 77385.0 34 89243.5 35 101820.5 36 115690.5 37 130160.5 38 142406.0 39 151648.5 40 158625.5 41 161667.5 42 160975.5 43 159508.0 44 155245.5 45 147419.0 46 138607.5 47 128736.5 48 117152.0 49 105321.0 50 94608.5 51 84703.5 52 74481.5 53 64252.0 54 54493.5 55 45535.0 56 37714.0 57 30963.5 58 25322.0 59 20470.0 60 16288.5 61 12709.0 62 9667.0 63 7240.0 64 5428.5 65 4069.0 66 3044.0 67 2189.5 68 1635.0 69 1250.5 70 907.0 71 656.5 72 457.5 73 353.5 74 268.0 75 180.0 76 129.0 77 103.5 78 82.5 79 63.5 80 54.0 81 37.0 82 25.5 83 29.5 84 24.0 85 15.5 86 11.5 87 8.5 88 6.5 89 4.0 90 2.5 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14729823772577838 2 0.0 3 0.0 4 0.01632437061183867 5 1.5787592467929085E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.3681388701893627E-4 24-25 0.0 26-27 0.0 28-29 1.1051314727550359E-4 30-31 8.841051782040287E-4 32-33 0.0011840694350946815 34-35 2.052387020830781E-4 36-37 0.0011840694350946815 38-39 0.007278080127715309 40-41 0.01619806987209524 42-43 0.005620382918582754 44-45 0.0020839622057666394 46-47 3.6311462676236894E-4 48-49 0.006615001244062287 50-51 0.002683890719547944 52-53 0.010293510289089763 54-55 0.02036599428362852 56-57 0.01809258096824673 58-59 0.017366351714721995 60-61 0.02316039815045197 62-63 0.006172948654960272 64-65 0.009551493443097097 66-67 0.02671260645573601 68-69 0.039153229320464125 70-71 0.0369113911900182 72-73 0.026823119603011515 74-75 0.03654827656325583 76-77 0.030059576058936978 78-79 0.011619668056395806 80-81 0.009456767888289521 82-83 0.0038679601546426257 84-85 0.006662364021466073 86-87 0.022481531674331017 88-89 0.02000287965686615 90-91 0.014224620813604105 92-93 0.029570160692431173 94-95 0.03089631845973722 96-97 0.03228562659691498 98-99 0.021786877605742137 100-101 0.022355230934587585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3167044.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.81021637050355 #Duplication Level Percentage of deduplicated Percentage of total 1 97.76165894016869 87.79995742155923 2 1.5474625873819958 2.779558995960726 3 0.25379720356611857 0.6838074529950553 4 0.12037784190509027 0.43244640110841703 5 0.06428591689018606 0.288676605274191 6 0.05811215934635255 0.31314393627918613 7 0.024937923479554806 0.15677762124709146 8 0.023530049109734708 0.16905910414030792 9 0.0179511832363932 0.14509796855103266 >10 0.11544004983588557 2.0751071060123394 >50 0.009281429960945635 0.5480871066398705 >100 0.002737038387027047 0.4149482707806695 >500 3.2073052947807437E-4 0.22809589683497894 >1k 7.191319081572345E-5 0.13433692841866668 >5k 0.0 0.0 >10k+ 3.503301173684409E-5 3.830899184198202 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 121758 3.844531367420219 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0017682103564080575 0.0 0.0 2 0.0019892366509590646 0.0 3.157518493585817E-5 3 0.0023365636852535044 0.0 3.157518493585817E-5 4 0.0024628644249969373 3.157518493585817E-5 3.157518493585817E-5 5 0.0028417666442272353 3.157518493585817E-5 6.315036987171633E-5 6 0.0034416951580085405 3.157518493585817E-5 6.315036987171633E-5 7 0.0046099770006352925 3.157518493585817E-5 6.315036987171633E-5 8 0.005651958103518612 3.157518493585817E-5 9.472555480757451E-5 9 0.006409762541979208 3.157518493585817E-5 9.472555480757451E-5 10-11 0.009409405110885734 3.157518493585817E-5 1.1051314727550359E-4 12-13 0.012377472494856403 3.157518493585817E-5 1.5787592467929085E-4 14-15 0.015361327471295 3.157518493585817E-5 1.5787592467929085E-4 16-17 0.018376757632669456 3.157518493585817E-5 1.5787592467929085E-4 18-19 0.021881603160549712 3.157518493585817E-5 1.5787592467929085E-4 20-21 0.025654837760384762 3.157518493585817E-5 1.5787592467929085E-4 22-23 0.030154301613744553 3.157518493585817E-5 1.5787592467929085E-4 24-25 0.03534841953569322 3.157518493585817E-5 1.7366351714721994E-4 26-27 0.04103195282414769 3.157518493585817E-5 2.2102629455100718E-4 28-29 0.047725892030549624 3.157518493585817E-5 2.683890719547944E-4 30-31 0.07942737770615121 3.157518493585817E-5 2.841766644227235E-4 32-33 0.08817370393338395 3.157518493585817E-5 3.157518493585817E-4 34-35 0.09583068628032954 3.157518493585817E-5 3.157518493585817E-4 36-37 0.10487697676445291 3.157518493585817E-5 3.157518493585817E-4 38-39 0.11561253964264469 3.157518493585817E-5 3.473270342944399E-4 40-41 0.12775319825048215 3.157518493585817E-5 3.7890221923029806E-4 42-43 0.14185151832434284 4.736277740378725E-5 3.7890221923029806E-4 44-45 0.15658134209692065 6.315036987171633E-5 4.104774041661562E-4 46-47 0.1713269534619664 6.315036987171633E-5 4.4205258910201436E-4 48-49 0.18766711166627303 6.315036987171633E-5 4.4205258910201436E-4 50-51 0.2054597283776291 6.315036987171633E-5 4.7362777403787255E-4 52-53 0.22421538822952886 6.315036987171633E-5 4.894153665058016E-4 54-55 0.24412354233158745 9.472555480757451E-5 5.052029589737307E-4 56-57 0.2662103841942203 9.472555480757451E-5 5.052029589737307E-4 58-59 0.2903969758550876 9.472555480757451E-5 5.052029589737307E-4 60-61 0.31703064434848394 9.472555480757451E-5 5.052029589737307E-4 62-63 0.34544831079075633 9.472555480757451E-5 5.367781439095889E-4 64-65 0.37607624017853875 9.472555480757451E-5 5.367781439095889E-4 66-67 0.40968802454275977 9.472555480757451E-5 5.52565736377518E-4 68-69 0.4473730077637065 9.472555480757451E-5 5.68353328845447E-4 70-71 0.4878050320740729 9.472555480757451E-5 5.68353328845447E-4 72-73 0.5328470333850746 9.472555480757451E-5 6.315036987171634E-4 74-75 0.5805255626382204 9.472555480757451E-5 6.630788836530215E-4 76-77 0.6338876251798207 9.472555480757451E-5 6.630788836530215E-4 78-79 0.695696049691763 9.472555480757451E-5 6.630788836530215E-4 80-81 0.7599199758512986 9.472555480757451E-5 6.630788836530215E-4 82-83 0.8342637487827766 9.472555480757451E-5 6.630788836530215E-4 84-85 0.9181905903422876 9.472555480757451E-5 7.262292535247379E-4 86-87 1.007800965190253 9.472555480757451E-5 7.262292535247379E-4 88-89 1.1081469029164104 9.472555480757451E-5 7.42016845992667E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 18770 0.0 70.77722 4 TCGGAAG 18820 0.0 70.76585 3 GATCGGA 19140 0.0 69.98216 1 ATCGGAA 19095 0.0 69.8462 2 GAAGAGC 19700 0.0 67.37923 6 AGAGCAC 20080 0.0 65.98588 8 GAGCACA 20050 0.0 65.9188 9 AAGAGCA 21625 0.0 61.38131 7 GGAAGAG 23145 0.0 57.51437 5 ATGCCGT 14525 0.0 44.393547 48-49 CGTCTTC 14605 0.0 44.233784 52-53 GCCGTCT 14610 0.0 44.218296 50-51 TCGTATG 14835 0.0 43.98759 44-45 TCTCGTA 14420 0.0 43.77178 42-43 TGCCGTC 14580 0.0 43.722836 50-51 TATGCCG 14660 0.0 43.61213 48-49 CCGTCTT 14630 0.0 43.589985 52-53 CGTATGC 14745 0.0 43.403557 46-47 AATCTCG 14475 0.0 43.396645 40-41 CTCGTAT 14400 0.0 43.23856 44-45 >>END_MODULE