FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3167044
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1217583.844531367420219TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA187700.070.777224
TCGGAAG188200.070.765853
GATCGGA191400.069.982161
ATCGGAA190950.069.84622
GAAGAGC197000.067.379236
AGAGCAC200800.065.985888
GAGCACA200500.065.91889
AAGAGCA216250.061.381317
GGAAGAG231450.057.514375
ATGCCGT145250.044.39354748-49
CGTCTTC146050.044.23378452-53
GCCGTCT146100.044.21829650-51
TCGTATG148350.043.9875944-45
TCTCGTA144200.043.7717842-43
TGCCGTC145800.043.72283650-51
TATGCCG146600.043.6121348-49
CCGTCTT146300.043.58998552-53
CGTATGC147450.043.40355746-47
AATCTCG144750.043.39664540-41
CTCGTAT144000.043.2385644-45
Produced by FastQC (version 0.11.2)