FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088702
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT756412.4489575232573424TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG104550.072.415853
GATCGGA106100.071.688551
CGGAAGA105250.071.663454
ATCGGAA107150.070.614342
GAAGAGC116250.064.869796
CGGCGAC504.2024658E-757.0818441
TCGGACG654.42451E-851.1509863
GGAAGAG149800.050.5948145
TTGTGTT230700.050.516731
AGAGCAC157800.047.758968
GAGCACA159000.047.4582449
TGCCGTC84850.044.31162350-51
AAGAGCA170050.044.2905967
GCCGTCT85150.044.2670850-51
TATGCCG84850.044.17022748-49
TCTCGTA84800.044.05052642-43
CGTATGC85300.044.04252246-47
TCGTATG85750.043.97648644-45
CGTCTTC85900.043.85328352-53
ATCTCGT85200.043.7879742-43
Produced by FastQC (version 0.11.2)