##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_50330665-2.34100000035758.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474467 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68260639563995 33.0 33.0 33.0 33.0 33.0 2 32.71972832937356 33.0 33.0 33.0 33.0 33.0 3 32.74561269154125 33.0 33.0 33.0 33.0 33.0 4 36.68669899416723 37.0 37.0 37.0 37.0 37.0 5 36.68938240033106 37.0 37.0 37.0 37.0 37.0 6 36.56288606798959 37.0 37.0 37.0 37.0 37.0 7 36.52298899116456 37.0 37.0 37.0 37.0 37.0 8 36.43464228862216 37.0 37.0 37.0 37.0 37.0 9 36.45790467199603 37.0 37.0 37.0 37.0 37.0 10-11 36.413197064256664 37.0 37.0 37.0 37.0 37.0 12-13 36.388686331238205 37.0 37.0 37.0 37.0 37.0 14-15 38.22909923632039 40.0 37.0 40.0 37.0 40.0 16-17 38.212457066511696 40.0 37.0 40.0 37.0 40.0 18-19 38.23100106002626 40.0 37.0 40.0 37.0 40.0 20-21 38.18219923724988 40.0 37.0 40.0 37.0 40.0 22-23 38.09512452580697 40.0 37.0 40.0 37.0 40.0 24-25 38.08334299871447 40.0 37.0 40.0 37.0 40.0 26-27 38.01439542333763 40.0 37.0 40.0 37.0 40.0 28-29 37.89918192483472 40.0 37.0 40.0 33.0 40.0 30-31 37.8740827822719 40.0 37.0 40.0 33.0 40.0 32-33 37.766247236273514 40.0 37.0 40.0 33.0 40.0 34-35 37.701781434143186 40.0 37.0 40.0 33.0 40.0 36-37 37.61252968821164 40.0 37.0 40.0 33.0 40.0 38-39 37.511088650606375 40.0 37.0 40.0 33.0 40.0 40-41 37.43733276701609 40.0 37.0 40.0 33.0 40.0 42-43 37.3322933787357 40.0 37.0 40.0 33.0 40.0 44-45 37.19051840254891 40.0 37.0 40.0 33.0 40.0 46-47 37.139165525343444 40.0 37.0 40.0 33.0 40.0 48-49 36.97223624319904 37.0 37.0 40.0 33.0 40.0 50-51 36.84040886380784 37.0 37.0 40.0 33.0 40.0 52-53 36.72770418841714 37.0 37.0 40.0 33.0 40.0 54-55 36.560620125465405 37.0 37.0 40.0 33.0 40.0 56-57 36.37159638823229 37.0 37.0 40.0 33.0 40.0 58-59 36.17435815470564 37.0 37.0 40.0 33.0 40.0 60-61 35.92413760215837 37.0 37.0 40.0 33.0 40.0 62-63 35.69613819865046 37.0 35.0 40.0 27.0 40.0 64-65 35.46387747341144 37.0 33.0 40.0 27.0 40.0 66-67 35.13071643307427 37.0 33.0 37.0 27.0 40.0 68-69 34.85912643005544 37.0 33.0 37.0 27.0 40.0 70-71 34.57577631869813 37.0 33.0 37.0 27.0 40.0 72-73 34.30800208691407 37.0 33.0 37.0 27.0 40.0 74-75 33.922374798289894 37.0 33.0 37.0 27.0 38.5 76-77 31.253171895200058 33.0 30.0 35.0 24.5 37.0 78-79 33.64433451729201 37.0 33.0 37.0 27.0 37.0 80-81 34.00761376086244 37.0 33.0 37.0 27.0 37.0 82-83 33.94238173311666 37.0 33.0 37.0 27.0 37.0 84-85 33.77662684529638 37.0 33.0 37.0 27.0 37.0 86-87 33.58290674314913 37.0 33.0 37.0 27.0 37.0 88-89 33.468434818488184 37.0 33.0 37.0 27.0 37.0 90-91 33.33908292169586 37.0 33.0 37.0 27.0 37.0 92-93 33.12605320660975 37.0 33.0 37.0 27.0 37.0 94-95 33.01228749464026 35.0 33.0 37.0 27.0 37.0 96-97 32.85288569215108 33.0 33.0 37.0 27.0 37.0 98-99 32.75713557707579 33.0 33.0 37.0 27.0 37.0 100-101 31.219172856215096 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16661861167828818 2306 2 0.17219709128709582 2306 3 0.1535460918635465 2306 4 0.195508106387706 2306 5 0.19116506227559427 2306 6 0.11595333403416674 2306 7 0.23689165151429137 2306 8 0.2768665624396931 2306 9 0.19915707868565846 2306 10-11 0.2855740792599377 2306 12-13 0.2542706031688695 2306 14-15 0.5033615996630658 2306 16-17 0.44297952844615196 2306 18-19 0.5176742826095548 2306 20-21 0.501671371236931 2306 22-23 0.6229735264076979 2306 24-25 0.6001103242817223 2306 26-27 0.5832099267979984 2306 28-29 0.6789033302344123 2306 30-31 0.6089689730400423 2306 32-33 0.569979284259226 2306 34-35 0.6442769599188907 2306 36-37 0.538516040097079 2306 38-39 0.5247186056314845 2306 40-41 0.5872960362666646 2306 42-43 0.4633322500322734 2306 44-45 0.7108020539520155 2306 46-47 0.7211186518788537 2306 48-49 0.5800985640683507 2306 50-51 0.6533941879768577 2306 52-53 0.5596692447075 2306 54-55 0.5986124080479485 2306 56-57 0.7027866251530739 2306 58-59 0.7406572499097024 2306 60-61 0.6795690857518579 2306 62-63 0.6476236447838346 2306 64-65 0.5759725736774399 2306 66-67 0.6455156468637782 2306 68-69 0.6847450754216524 2306 70-71 0.4504820389828126 2306 72-73 0.41854755872533644 2306 74-75 0.4629950663759459 2306 76-77 -0.022120128944450812 2306 78-79 0.5008616304909168 2306 80-81 0.4428697238126418 2306 82-83 0.5636408562280195 2306 84-85 0.5283913084276293 2306 86-87 0.49478689300814693 2306 88-89 0.6417973621243718 2306 90-91 0.5807621425511442 2306 92-93 0.4100918594841829 2306 94-95 0.44816255098577074 2306 96-97 0.4438298520381423 2306 98-99 0.5886182317524202 2306 100-101 0.4139085006942089 2307 1 0.10597067346346734 2307 2 0.14007909196367763 2307 3 0.12569813637421134 2307 4 0.13402703277668593 2307 5 0.11602632633812959 2307 6 0.11838076308270473 2307 7 0.11466187169236264 2307 8 0.2133161460519375 2307 9 0.10331951669535044 2307 10-11 0.17974519051499982 2307 12-13 0.15440592019218258 2307 14-15 0.35152852184857863 2307 16-17 0.36955564787062656 2307 18-19 0.35248854559962695 2307 20-21 0.4266055121537775 2307 22-23 0.44772178636034 2307 24-25 0.39075311613103025 2307 26-27 0.3099550125587598 2307 28-29 0.4627613769187846 2307 30-31 0.4030396761847257 2307 32-33 0.2589174774890495 2307 34-35 0.5051208558975304 2307 36-37 0.5759468520711977 2307 38-39 0.4720358376785825 2307 40-41 0.38535607239919045 2307 42-43 0.4709240388801419 2307 44-45 0.5128847124996625 2307 46-47 0.6037185381549648 2307 48-49 0.41319617226798755 2307 50-51 0.4618972074178771 2307 52-53 0.5185523034480397 2307 54-55 0.4989321664926649 2307 56-57 0.5089224964003662 2307 58-59 0.5643879330447277 2307 60-61 0.31851645606703727 2307 62-63 0.4199023690610062 2307 64-65 0.3212445752897324 2307 66-67 0.2391183186552155 2307 68-69 0.23393245351036995 2307 70-71 0.21903684597611317 2307 72-73 0.326128011173644 2307 74-75 0.25042317920730994 2307 76-77 0.013029907404003893 2307 78-79 0.30149394966634446 2307 80-81 0.29339119819237425 2307 82-83 0.3154519810147889 2307 84-85 0.3671148298372273 2307 86-87 0.33159132392088964 2307 88-89 0.23667043043787572 2307 90-91 0.1422072275921309 2307 92-93 0.35779776808761454 2307 94-95 0.08895793437200439 2307 96-97 0.11459921568824427 2307 98-99 0.12203451873966742 2307 100-101 -0.03419778075080515 2201 1 0.1253991416916378 2201 2 0.1451498040861523 2201 3 0.11883530955639543 2201 4 0.1255457457882727 2201 5 0.1408103001093437 2201 6 0.10317557289626933 2201 7 0.20197536673278904 2201 8 0.211051691049299 2201 9 0.16973195313779144 2201 10-11 0.18644825517005614 2201 12-13 0.1937838202227553 2201 14-15 0.4061289775180512 2201 16-17 0.380537560015334 2201 18-19 0.3636996817169873 2201 20-21 0.4216285734746634 2201 22-23 0.3481135509681579 2201 24-25 0.49333431698679675 2201 26-27 0.4810984928732793 2201 28-29 0.44660108684626465 2201 30-31 0.4325401068479451 2201 32-33 0.5103240791394583 2201 34-35 0.4207455954157453 2201 36-37 0.49584349289806795 2201 38-39 0.530998495009591 2201 40-41 0.5467679531471532 2201 42-43 0.5547887550591852 2201 44-45 0.5171496676273293 2201 46-47 0.6913619767924075 2201 48-49 0.6173444315550327 2201 50-51 0.6572446553909828 2201 52-53 0.5919248132421586 2201 54-55 0.7120618377993821 2201 56-57 0.6696154474158931 2201 58-59 0.7368338222948267 2201 60-61 0.6413402175629699 2201 62-63 0.6138189037502357 2201 64-65 0.5538756497250077 2201 66-67 0.5975204191803485 2201 68-69 0.6106273216718492 2201 70-71 0.5750182024263353 2201 72-73 0.48600478592288 2201 74-75 0.45694355930233854 2201 76-77 0.39865409122774054 2201 78-79 0.6060318897069124 2201 80-81 0.5096501240349482 2201 82-83 0.6419085951683243 2201 84-85 0.7454872007077213 2201 86-87 0.6880623861308806 2201 88-89 0.6140703359698065 2201 90-91 0.4987720632454895 2201 92-93 0.4126552324537016 2201 94-95 0.550587230584938 2201 96-97 0.6296099128069699 2201 98-99 0.6820981457575357 2201 100-101 0.6588782660386698 1101 1 0.11483538026106288 1101 2 0.05585401674908752 1101 3 0.06861138766583252 1101 4 0.10486129311442483 1101 5 0.12337256357272963 1101 6 0.10680047868502385 1101 7 0.08462765614166301 1101 8 0.10306224136398612 1101 9 0.007554145508244403 1101 10-11 0.06742861015062829 1101 12-13 0.08224426225753945 1101 14-15 0.16311960312106066 1101 16-17 0.21640325125525095 1101 18-19 0.21622689624431501 1101 20-21 0.30055308950957027 1101 22-23 0.2235303159641333 1101 24-25 0.18788129284787658 1101 26-27 0.2557668793783989 1101 28-29 0.18844277968366185 1101 30-31 0.2408677085070039 1101 32-33 0.16528148994217418 1101 34-35 0.22343548191850715 1101 36-37 0.14186050033882935 1101 38-39 0.25244467206688626 1101 40-41 0.15460377379179846 1101 42-43 0.17426390850333462 1101 44-45 0.317948773956779 1101 46-47 0.3252429623580042 1101 48-49 0.2428556599217444 1101 50-51 0.3555701660814776 1101 52-53 0.3284894998086614 1101 54-55 0.33121998427341026 1101 56-57 0.2581409044538887 1101 58-59 0.28136876332077065 1101 60-61 0.11671923082750624 1101 62-63 0.19252891061479005 1101 64-65 0.17101006135267482 1101 66-67 0.12630115591395707 1101 68-69 0.18658125044041896 1101 70-71 -0.04831213228327158 1101 72-73 0.11872531755332005 1101 74-75 -0.004651533712149103 1101 76-77 -0.05767071888744013 1101 78-79 0.312900284414539 1101 80-81 0.17650521319021806 1101 82-83 0.18408127958872456 1101 84-85 0.19786236618670472 1101 86-87 0.28478850757245766 1101 88-89 0.24598809703328328 1101 90-91 0.21151971023352445 1101 92-93 0.41385081600537177 1101 94-95 0.2684750632321595 1101 96-97 0.12345483420739356 1101 98-99 0.30361686799421506 1101 100-101 0.3078712058261708 2304 1 0.055743720781734396 2304 2 0.10750591339566995 2304 3 0.07949412505391962 2304 4 0.1256974592016391 2304 5 0.06493462925159577 2304 6 -0.01955109642538133 2304 7 0.09949295600665664 2304 8 0.08211425386813431 2304 9 0.041211727843375456 2304 10-11 0.09081665326605304 2304 12-13 0.08218438156237795 2304 14-15 0.22743428179826708 2304 16-17 0.16272450886894063 2304 18-19 0.21337065325175786 2304 20-21 0.14899008757452492 2304 22-23 0.1633029082722075 2304 24-25 0.1549991820566774 2304 26-27 0.21481774720185598 2304 28-29 0.22469177353909942 2304 30-31 0.08994751425534986 2304 32-33 0.23601200464818106 2304 34-35 0.2530358990322128 2304 36-37 0.24202221129900892 2304 38-39 0.31242213575222166 2304 40-41 0.3788875791735862 2304 42-43 0.26943004765746537 2304 44-45 0.23518554784958923 2304 46-47 0.2869366861663991 2304 48-49 0.29976084694635574 2304 50-51 0.37218937481598857 2304 52-53 0.427367911230057 2304 54-55 0.46528574783332743 2304 56-57 0.4011917497807289 2304 58-59 0.33781806306400597 2304 60-61 0.2788304984224297 2304 62-63 0.3564513211138305 2304 64-65 0.4557123302355208 2304 66-67 0.3370190063491876 2304 68-69 0.0711913162860256 2304 70-71 -0.07442425077955761 2304 72-73 0.022221622318930656 2304 74-75 0.054305642327499015 2304 76-77 -0.12778671209478532 2304 78-79 0.24535221361504966 2304 80-81 0.26001992548001596 2304 82-83 0.1629493491839895 2304 84-85 0.23224546434770588 2304 86-87 0.3203564825826888 2304 88-89 0.4001686462616689 2304 90-91 0.2705192328421191 2304 92-93 0.20155075880442297 2304 94-95 0.3256483900547025 2304 96-97 0.16396825988933728 2304 98-99 -0.09129416616524111 2304 100-101 0.18351273153335512 2202 1 0.07432256856970554 2202 2 0.05710401777300689 2202 3 0.03545632555760392 2202 4 0.09111210098696887 2202 5 0.08035838460742184 2202 6 0.08577353922433417 2202 7 0.08393057169123352 2202 8 0.010846132624273253 2202 9 0.07392816230653665 2202 10-11 8.528281609443411E-5 2202 12-13 0.04980553428784873 2202 14-15 0.07339953727347392 2202 16-17 0.060304663363218936 2202 18-19 0.07023111806952187 2202 20-21 0.05593283124701287 2202 22-23 -0.047908580474455675 2202 24-25 -0.12818384154362406 2202 26-27 -0.15153495054337185 2202 28-29 -0.03125964471217202 2202 30-31 0.010940559702540043 2202 32-33 0.04614680847536334 2202 34-35 -0.01743818633755012 2202 36-37 -0.032176885558989454 2202 38-39 0.07542180971744727 2202 40-41 0.001599880439982826 2202 42-43 -0.029796710812377114 2202 44-45 0.15184130390369432 2202 46-47 0.16501893421468594 2202 48-49 0.1107916046049624 2202 50-51 0.12614350916798855 2202 52-53 0.10216490965636638 2202 54-55 0.07888319500670349 2202 56-57 0.08012579576959666 2202 58-59 0.11275009636577238 2202 60-61 0.14464793435146106 2202 62-63 0.08322904498034944 2202 64-65 0.0016421583099415216 2202 66-67 -0.03467347354320083 2202 68-69 -0.02530470840019916 2202 70-71 -0.28688853456844043 2202 72-73 -0.13577945077190634 2202 74-75 -0.2039241685466564 2202 76-77 -0.10788960558923577 2202 78-79 0.018098273600315906 2202 80-81 0.008889714378916835 2202 82-83 0.061200827251091994 2202 84-85 0.0993542033632906 2202 86-87 0.09795840695802838 2202 88-89 0.04405395390242717 2202 90-91 -0.029304400314849488 2202 92-93 0.007461230168559041 2202 94-95 0.13918473779774843 2202 96-97 0.1323711259147089 2202 98-99 -0.11447857605513434 2202 100-101 -0.009350957691328432 1102 1 0.07024084764066174 1102 2 0.04390003068927939 1102 3 0.060038186192308274 1102 4 0.05137430633528339 1102 5 0.08350727807088276 1102 6 0.0014085795445453186 1102 7 0.07963388536965255 1102 8 0.16188378848783458 1102 9 0.09642066418693673 1102 10-11 0.056174319559225694 1102 12-13 0.12400606073460096 1102 14-15 -0.010399172104015975 1102 16-17 0.06663991631129562 1102 18-19 0.09049619399228703 1102 20-21 0.008123457488828478 1102 22-23 0.08320938593545435 1102 24-25 0.08689558751354554 1102 26-27 0.07795808285207073 1102 28-29 -0.005144954121426792 1102 30-31 0.1226988608292956 1102 32-33 0.060712679966691496 1102 34-35 0.0700696591346599 1102 36-37 0.026973114189779324 1102 38-39 0.07874612717501606 1102 40-41 0.06418102496030542 1102 42-43 0.04612310185314783 1102 44-45 0.12002009044589812 1102 46-47 0.03061859481404383 1102 48-49 0.06808077740546281 1102 50-51 -0.08552771349792465 1102 52-53 -0.03687062102087424 1102 54-55 -0.04541431709656507 1102 56-57 0.0022563300088407345 1102 58-59 -0.010160626802715456 1102 60-61 -0.0014563312915853999 1102 62-63 -0.1342407817649729 1102 64-65 -0.1475145503689177 1102 66-67 0.03291391786268605 1102 68-69 0.058163251379433234 1102 70-71 -0.11054689601221668 1102 72-73 -0.10566485280853044 1102 74-75 -0.09346319667151448 1102 76-77 0.025877962206237726 1102 78-79 0.12171847431768867 1102 80-81 0.048777154654480626 1102 82-83 0.018187322661994187 1102 84-85 0.06937810952477719 1102 86-87 0.09854890640520608 1102 88-89 0.1149427243855925 1102 90-91 -0.009593559352317982 1102 92-93 0.10380996433043066 1102 94-95 0.013955927454247785 1102 96-97 0.18835257073119038 1102 98-99 -0.15758962337569926 1102 100-101 -0.14276156059716172 2305 1 0.05177162398685198 2305 2 -0.013686506887097494 2305 3 0.022903274206747426 2305 4 -0.01764828961155729 2305 5 0.001956874555979482 2305 6 -0.010770300579771686 2305 7 0.07294771762163066 2305 8 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-0.2493708693708072 1213 92-93 -0.23792514034092704 1213 94-95 -0.514269115316857 1213 96-97 -0.3779151821108755 1213 98-99 -0.1300183557023331 1213 100-101 0.06418352435720465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 10.0 9 22.0 10 21.0 11 13.0 12 25.0 13 59.0 14 160.0 15 365.0 16 781.0 17 1273.0 18 1998.0 19 2778.0 20 3854.0 21 4839.0 22 6487.0 23 8063.0 24 10150.0 25 13170.0 26 16968.0 27 22413.0 28 30428.0 29 37449.0 30 41837.0 31 52976.0 32 70827.0 33 101183.0 34 154925.0 35 269046.0 36 524737.0 37 831479.0 38 265766.0 39 364.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.023476523274354 25.988462290356384 23.590783269875796 13.397277916493469 2 31.448105794096264 22.846051291045708 33.670927921043194 12.03491499381483 3 38.5058277196665 18.820659156093008 27.1000987283322 15.57341439590829 4 30.65793887853716 18.9592132865013 34.596679991431195 15.786167843530349 5 30.602030341973602 17.447564317063115 36.49612440694131 15.45428093402197 6 25.496803958185744 18.30430553327242 40.72165844199983 15.477232066542006 7 34.10997196567988 19.080957636533444 29.722279585866367 17.086790811920306 8 30.221215316268108 28.689612752968618 24.817627392080798 16.271544538682473 9 29.84966055316155 26.61308475724267 25.808830750218124 17.728423939377652 10-11 24.209233746095624 28.97098647910843 30.885944326596395 15.933835448199552 12-13 24.205051027150493 27.737832026048437 30.032346359842343 18.024770586958727 14-15 22.883877618897323 28.430506448459408 30.984329150479677 17.701286782163596 16-17 21.80287714485584 29.112067366426793 31.99402133873679 17.091034149980583 18-19 20.48556315360035 29.376932486874953 32.32688090000796 17.810623459516737 20-21 21.052372086594808 29.37418442032163 31.853789119030484 17.71965437405308 22-23 20.363016357296324 30.14086095095842 31.654806510426614 17.841316181318643 24-25 20.399968963013045 29.196045047276847 31.814952472593088 18.58903351711702 26-27 20.288955156807507 29.311160746940658 31.022721256739334 19.37716283951251 28-29 20.405283648239852 29.923484454919436 30.777608650275646 18.893623246565063 30-31 19.443360584633645 30.142212061443434 30.929825089611242 19.484602264311683 32-33 19.235849645646308 30.848543374253794 31.103995680629247 18.811611299470645 34-35 19.795284669122825 30.025332794764758 31.748961036153133 18.430421499959284 36-37 20.172743302175903 30.300356891283343 31.407968645619604 18.118931160921154 38-39 20.639392019037395 30.537188880195032 30.65728403484154 18.166135065926035 40-41 20.337518719041586 30.255730906577583 30.609154495500356 18.797595878880475 42-43 19.794017257576638 30.669915757111966 31.33114432634081 18.204922658970588 44-45 19.553984187803277 30.179104670631773 31.553791011142412 18.713120130422535 46-47 20.328028053068696 29.853648462912812 30.884421728761875 18.933901755256617 48-49 19.98002692444948 30.77245930314093 30.615022808185323 18.632490964224267 50-51 20.48760754087442 30.102351754439848 30.221107432565685 19.18893327212004 52-53 19.897365821088584 29.916990151863747 29.995418766567063 20.190225260480606 54-55 20.713146985208994 30.00037187704128 30.23410872391202 19.052372413837706 56-57 20.126948184954617 30.28949254881212 30.155398046444194 19.428161219789075 58-59 20.12744072080462 30.330378215474212 30.611904626208513 18.930276437512656 60-61 20.430771668540913 30.665317641960865 30.289330227827797 18.614580461670425 62-63 19.841452995252766 30.626238238001974 30.210885420497746 19.321423346247517 64-65 20.416781602923777 30.24617072808456 30.318881172582223 19.018166496409442 66-67 20.368800960303794 30.485562598924204 29.880845282390396 19.26479115838161 68-69 20.379199131595787 30.742216755390384 29.986264416551023 18.89231969646281 70-71 19.692648528786027 31.43626423938849 30.025600831193195 18.84548640063229 72-73 19.56779968821039 31.473429263312653 29.927093458259385 19.031677590217573 74-75 20.07595167505442 30.709731957737606 29.7636005629973 19.450715804210674 76-77 20.07597034193526 30.316301890041693 30.01198393437275 19.595743833650296 78-79 19.9072859673579 30.422380542623035 30.325620703857098 19.34471278616197 80-81 19.32589823584165 30.70138318841845 30.171739596986676 19.80097897875323 82-83 18.772045006721992 30.640758359345305 30.137351695390468 20.449844938542235 84-85 18.838173217348288 30.45179323557982 30.575485559562775 20.134547987509116 86-87 18.943215807096607 30.391832496160415 31.18792320551162 19.47702849123136 88-89 19.155764757240938 30.80266213411319 30.701872103575045 19.33970100507083 90-91 19.45683287781084 30.51030644901826 30.545531635572303 19.48732903759859 92-93 18.851272112960284 31.471682919523197 30.375216425147723 19.3018285423688 94-95 18.647324110374736 31.67262404787681 30.297588776568695 19.38246306517976 96-97 19.12912568138121 31.727704624579367 30.015115303203523 19.128054390835896 98-99 19.39744666884002 30.996030365751643 29.779128588379788 19.827394377028547 100-101 19.273311188744056 30.950597085362443 29.493682526625552 20.282409199267956 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 131.0 1 94.5 2 77.0 3 183.0 4 238.0 5 168.0 6 131.5 7 143.5 8 142.0 9 139.5 10 147.5 11 144.5 12 152.0 13 160.5 14 170.0 15 206.5 16 254.5 17 324.0 18 454.0 19 669.5 20 1000.5 21 1602.0 22 2508.5 23 3878.0 24 5688.5 25 8233.5 26 11936.5 27 16974.0 28 22616.5 29 28345.5 30 34848.5 31 41763.5 32 50416.0 33 62578.0 34 78385.5 35 97851.5 36 125359.0 37 156037.0 38 180925.5 39 189747.5 40 182908.5 41 166917.5 42 148474.0 43 137790.0 44 124507.0 45 108871.5 46 101851.0 47 90657.0 48 70340.0 49 52691.5 50 39716.5 51 31717.5 52 26112.5 53 19970.5 54 14525.0 55 10207.0 56 6986.5 57 4795.5 58 3296.5 59 2324.0 60 1599.0 61 1092.5 62 760.5 63 515.5 64 342.0 65 225.5 66 143.5 67 94.5 68 62.5 69 57.0 70 43.5 71 28.0 72 23.0 73 16.5 74 14.0 75 11.0 76 5.0 77 1.5 78 1.0 79 2.0 80 2.0 81 0.5 82 1.0 83 2.0 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1530026466305673 2 0.0 3 0.0 4 0.015235604273566792 5 2.8288920401848156E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.222700888716641E-4 24-25 0.0 26-27 0.0 28-29 1.0103185857802912E-4 30-31 0.001151763187789532 32-33 0.0013134141615143786 34-35 2.626828323028757E-4 36-37 0.0017781607109733128 38-39 0.007233881074186886 40-41 0.015437667990722851 42-43 0.005395101248066756 44-45 0.0019398116846981593 46-47 5.657784080369631E-4 48-49 0.005496133106644784 50-51 0.0023843518624414874 52-53 0.009800090282068826 54-55 0.021459166761973385 56-57 0.018812132067229023 58-59 0.018347385517770088 60-61 0.02347980393353397 62-63 0.007476357534774155 64-65 0.01089123435471154 66-67 0.026935093496902568 68-69 0.03897809103940364 70-71 0.036957453867843056 72-73 0.027622110135233163 74-75 0.037199930328430324 76-77 0.029844811023949803 78-79 0.012123823029363497 80-81 0.010345662318390183 82-83 0.005071799300617062 84-85 0.007375325676196125 86-87 0.022853406410350188 88-89 0.020691324636780365 90-91 0.015558906221016487 92-93 0.030228732086546313 94-95 0.031724003593501145 96-97 0.03293638589643749 98-99 0.022691755436625344 100-101 0.023217121101231093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2474467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.91718011932807 #Duplication Level Percentage of deduplicated Percentage of total 1 91.23877922280138 53.75531589337397 2 4.320523480825249 5.09106120259135 3 1.4135534123105422 2.4984774300417314 4 0.6820484115809594 1.6073747646086796 5 0.40605064860259643 1.1961679600644581 6 0.3161597770186723 1.117634551745743 7 0.2155001714801928 0.8887663693191222 8 0.14719211427142606 0.6937715446939463 9 0.13325345984770448 0.7065826285833771 >10 0.9000313186820587 10.277602086888793 >50 0.11064365355566538 4.565709864394163 >100 0.10955578589925492 13.178912280969193 >500 0.005529293821930379 2.159917517291893 >1k 9.729159797816502E-4 0.8598368580763482 >5k 1.3755554841565873E-4 0.565159289537813 >10k+ 6.877777420595918E-5 0.8377097578194906 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 20673 0.8354526449534385 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7276 0.29404312120549597 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6671 0.2695934114296129 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3469 0.1401918069628732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.4247646058726992E-4 0.0 0.0 2 2.4247646058726992E-4 0.0 0.0 3 2.8288920401848156E-4 0.0 0.0 4 3.233019474496932E-4 0.0 0.0 5 3.233019474496932E-4 0.0 0.0 6 4.041274343121165E-4 0.0 0.0 7 6.061911514681748E-4 0.0 0.0 8 7.274293817618097E-4 0.0 0.0 9 8.486676120554446E-4 0.0 0.0 10-11 0.0013538269049455903 0.0 0.0 12-13 0.001980224428129371 0.0 0.0 14-15 0.0028288920401848155 0.0 0.0 16-17 0.003980655227974347 0.0 0.0 18-19 0.005294069389488726 0.0 0.0 20-21 0.006809547268159162 0.0 2.0206371715605825E-5 22-23 0.008385644261976417 0.0 4.041274343121165E-5 24-25 0.010709377009271086 0.0 6.061911514681748E-5 26-27 0.013538269049455903 0.0 8.08254868624233E-5 28-29 0.016346954717925112 0.0 8.08254868624233E-5 30-31 0.02390413773956169 0.0 8.08254868624233E-5 32-33 0.026935093496902564 0.0 8.08254868624233E-5 34-35 0.030349970316839947 0.0 8.08254868624233E-5 36-37 0.034249800057951876 0.0 8.08254868624233E-5 38-39 0.038291074401073044 0.0 1.0103185857802914E-4 40-41 0.04273647617850632 0.0 1.2123823029363496E-4 42-43 0.04706063972564596 0.0 1.4144460200924078E-4 44-45 0.052172851769694245 0.0 1.616509737248466E-4 46-47 0.057345682928889335 0.0 1.8185734544045242E-4 48-49 0.06268016506180928 0.0 2.0206371715605825E-4 50-51 0.06856021923105056 0.0 2.0206371715605825E-4 52-53 0.07429882879828262 0.0 2.0206371715605825E-4 54-55 0.08086589960585451 0.0 2.222700888716641E-4 56-57 0.0884230826274911 0.0 2.4247646058726992E-4 58-59 0.09630356759657736 0.0 2.4247646058726992E-4 60-61 0.10505292654943468 0.0 2.4247646058726992E-4 62-63 0.11398414284773246 0.0 2.8288920401848156E-4 64-65 0.1238246458732325 0.0 3.43508319165299E-4 66-67 0.13481691208652208 0.0 3.6371469088090485E-4 68-69 0.14593041653010527 0.0 3.6371469088090485E-4 70-71 0.16076189336935995 0.0 3.6371469088090485E-4 72-73 0.17472449622484357 0.0 3.6371469088090485E-4 74-75 0.19258692882143913 0.0 3.6371469088090485E-4 76-77 0.21109596531293406 0.0 3.6371469088090485E-4 78-79 0.2341312290687247 0.0 3.6371469088090485E-4 80-81 0.25997517849298457 0.0 3.6371469088090485E-4 82-83 0.2898806086320812 0.0 3.6371469088090485E-4 84-85 0.32445371063748274 0.0 3.6371469088090485E-4 86-87 0.3625831340648309 0.0 3.6371469088090485E-4 88-89 0.4054812612170621 0.0 3.6371469088090485E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGG 30 1.2922076E-4 63.425327 1 TCGGAAG 4245 0.0 58.28829 3 CGGAAGA 4260 0.0 57.97156 4 GATCGGA 4290 0.0 57.65938 1 ATCGGAA 4320 0.0 57.166405 2 GAAGAGC 4695 0.0 52.696224 6 CGGCGAG 65 2.5648496E-6 43.90984 1 ACGGCGA 65 2.5648496E-6 43.90984 1 TATGCCG 2730 0.0 43.23522 48-49 TGCCGTC 2705 0.0 43.20019 50-51 ATGCCGT 2750 0.0 43.17986 48-49 GCCGTCT 2765 0.0 42.86405 50-51 CGTATGC 2775 0.0 42.7848 46-47 TCGTATG 2810 0.0 42.67441 44-45 CTCGTAT 2775 0.0 41.929108 44-45 GGAAGAG 6055 0.0 41.722935 5 CCGTCTT 2820 0.0 41.691994 52-53 AATCTCG 2775 0.0 41.590187 40-41 TCTCGTA 2875 0.0 40.80107 42-43 CGTCTTC 2925 0.0 40.763783 52-53 >>END_MODULE