FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474467
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT206730.8354526449534385TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC72760.29404312120549597No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG66710.2695934114296129No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA34690.1401918069628732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGG301.2922076E-463.4253271
TCGGAAG42450.058.288293
CGGAAGA42600.057.971564
GATCGGA42900.057.659381
ATCGGAA43200.057.1664052
GAAGAGC46950.052.6962246
CGGCGAG652.5648496E-643.909841
ACGGCGA652.5648496E-643.909841
TATGCCG27300.043.2352248-49
TGCCGTC27050.043.2001950-51
ATGCCGT27500.043.1798648-49
GCCGTCT27650.042.8640550-51
CGTATGC27750.042.784846-47
TCGTATG28100.042.6744144-45
CTCGTAT27750.041.92910844-45
GGAAGAG60550.041.7229355
CCGTCTT28200.041.69199452-53
AATCTCG27750.041.59018740-41
TCTCGTA28750.040.8010742-43
CGTCTTC29250.040.76378352-53
Produced by FastQC (version 0.11.2)