##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2852942 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6607084896924 33.0 33.0 33.0 33.0 33.0 2 32.672235187396026 33.0 33.0 33.0 33.0 33.0 3 32.7149665152674 33.0 33.0 33.0 33.0 33.0 4 36.62340384066693 37.0 37.0 37.0 37.0 37.0 5 36.641966783762165 37.0 37.0 37.0 37.0 37.0 6 36.52260859141195 37.0 37.0 37.0 37.0 37.0 7 36.343828230647524 37.0 37.0 37.0 37.0 37.0 8 36.277711569320374 37.0 37.0 37.0 37.0 37.0 9 36.377523272467506 37.0 37.0 37.0 37.0 37.0 10-11 36.27164993189487 37.0 37.0 37.0 35.0 37.0 12-13 36.262933841627344 37.0 37.0 37.0 35.0 37.0 14-15 38.04770566664166 40.0 37.0 40.0 35.0 40.0 16-17 38.03492780435074 40.0 37.0 40.0 35.0 40.0 18-19 37.97154305976076 40.0 37.0 40.0 35.0 40.0 20-21 38.0045584522924 40.0 37.0 40.0 33.0 40.0 22-23 37.672178929680314 40.0 37.0 40.0 33.0 40.0 24-25 37.87746105599062 40.0 37.0 40.0 33.0 40.0 26-27 37.8037839535469 40.0 37.0 40.0 33.0 40.0 28-29 37.51954280879177 40.0 37.0 40.0 33.0 40.0 30-31 37.4602706609528 40.0 37.0 40.0 33.0 40.0 32-33 37.40920775816683 38.5 37.0 40.0 33.0 40.0 34-35 37.25581978182522 40.0 37.0 40.0 33.0 40.0 36-37 37.200133756662424 40.0 37.0 40.0 33.0 40.0 38-39 37.160706912373264 40.0 37.0 40.0 33.0 40.0 40-41 37.10002113607638 38.5 37.0 40.0 33.0 40.0 42-43 36.395754803287275 37.0 37.0 40.0 33.0 40.0 44-45 36.16724595172282 37.0 37.0 40.0 33.0 40.0 46-47 36.611547658522326 37.0 37.0 40.0 33.0 40.0 48-49 36.286273783343646 37.0 37.0 40.0 33.0 40.0 50-51 36.295272213735856 37.0 37.0 40.0 33.0 40.0 52-53 36.29005847297281 37.0 37.0 40.0 33.0 40.0 54-55 35.87990835425326 37.0 35.0 40.0 30.0 40.0 56-57 35.752455360115974 37.0 35.0 40.0 30.0 40.0 58-59 35.633445755294005 37.0 33.0 40.0 27.0 40.0 60-61 35.33795446945644 37.0 33.0 40.0 27.0 40.0 62-63 34.92521228962944 37.0 33.0 37.0 27.0 40.0 64-65 34.63887418671673 37.0 33.0 37.0 27.0 40.0 66-67 33.74809722735338 37.0 33.0 37.0 27.0 40.0 68-69 33.50274856621691 37.0 33.0 37.0 27.0 37.0 70-71 32.99728175336197 37.0 33.0 37.0 24.5 37.0 72-73 31.686480832768417 33.0 30.0 37.0 18.5 37.0 74-75 30.430933401380052 33.0 27.0 37.0 15.0 37.0 76-77 27.839382293786553 30.0 24.5 33.0 15.0 35.0 78-79 29.698136169610176 33.0 27.0 37.0 15.0 37.0 80-81 30.004104009124617 33.0 27.0 37.0 10.5 37.0 82-83 29.812333023244076 33.0 27.0 37.0 6.0 37.0 84-85 29.625042850503092 33.0 27.0 37.0 6.0 37.0 86-87 29.407863531750735 33.0 27.0 37.0 6.0 37.0 88-89 29.315709187217966 33.0 27.0 37.0 6.0 37.0 90-91 29.180950751890506 33.0 27.0 37.0 6.0 37.0 92-93 28.92490366085255 33.0 27.0 37.0 6.0 37.0 94-95 28.7500583608079 33.0 27.0 37.0 6.0 37.0 96-97 28.563532662073044 33.0 27.0 37.0 6.0 37.0 98-99 28.455440734511953 33.0 24.5 37.0 6.0 37.0 100-101 26.966723648780803 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14758514055767336 2306 2 0.15822405749597834 2306 3 0.15437940369229608 2306 4 0.19852007669599203 2306 5 0.13559217749158847 2306 6 0.1819382793012707 2306 7 0.3095929676086655 2306 8 0.3179389406676094 2306 9 0.2788329853836302 2306 10-11 0.2749751321151379 2306 12-13 0.29954108803550383 2306 14-15 0.5124903509775507 2306 16-17 0.4888622447033413 2306 18-19 0.5762678377848829 2306 20-21 0.6300862447599442 2306 22-23 0.6269477411107616 2306 24-25 0.5547632271277649 2306 26-27 0.6449746692673273 2306 28-29 0.6726541987785524 2306 30-31 0.6179430631291396 2306 32-33 0.6368764833799645 2306 34-35 0.6022227901008748 2306 36-37 0.8006041059469027 2306 38-39 0.7948370004222127 2306 40-41 0.8231660525743791 2306 42-43 0.8220505974596861 2306 44-45 0.8162538807641013 2306 46-47 0.7958115840590949 2306 48-49 0.7701982737160407 2306 50-51 0.7960957707196457 2306 52-53 0.7081717683753652 2306 54-55 0.8371899881494969 2306 56-57 0.863344478326411 2306 58-59 0.9564588541046533 2306 60-61 0.820181531121932 2306 62-63 0.7596813111545231 2306 64-65 0.8079000452221621 2306 66-67 0.9242750013648191 2306 68-69 0.9888574800389236 2306 70-71 0.8001317560233403 2306 72-73 0.3361587671711419 2306 74-75 0.2525153068412038 2306 76-77 0.012332584554091142 2306 78-79 0.7848937661151467 2306 80-81 0.7914439897978376 2306 82-83 0.7207078284532962 2306 84-85 0.8638705480792872 2306 86-87 0.8014343226878289 2306 88-89 0.8797868147371801 2306 90-91 0.8610456011016687 2306 92-93 0.702538236578512 2306 94-95 0.6516075352275159 2306 96-97 0.723174254609571 2306 98-99 0.7553689468593667 2306 100-101 0.7911595685779815 2307 1 0.08922827188758475 2307 2 0.11905133449479166 2307 3 0.10516481576274117 2307 4 0.14261818680535754 2307 5 0.0939651304301492 2307 6 0.11647377894041 2307 7 0.19775252355191242 2307 8 0.23514249526911613 2307 9 0.1978309070866331 2307 10-11 0.2760913192399883 2307 12-13 0.2617473128001748 2307 14-15 0.434027273757998 2307 16-17 0.36134804111578234 2307 18-19 0.4967057052596715 2307 20-21 0.481699702033076 2307 22-23 0.5352690476160262 2307 24-25 0.4022575185218997 2307 26-27 0.44666022203693245 2307 28-29 0.5219117581119335 2307 30-31 0.46515975358030204 2307 32-33 0.4483997378058078 2307 34-35 0.42116128512402184 2307 36-37 0.4262095848118008 2307 38-39 0.43865676757351224 2307 40-41 0.5985117061227285 2307 42-43 0.4881922520704265 2307 44-45 0.57511599624479 2307 46-47 0.6217482635458822 2307 48-49 0.6152344218545522 2307 50-51 0.5765045861209046 2307 52-53 0.4528425177255073 2307 54-55 0.5357550837155785 2307 56-57 0.38913092157960705 2307 58-59 0.4858920833303557 2307 60-61 0.519425570503735 2307 62-63 0.46728960662144203 2307 64-65 0.454770694749449 2307 66-67 0.3774080182650934 2307 68-69 0.4630472486546111 2307 70-71 0.21422452144160076 2307 72-73 -0.023503698889847158 2307 74-75 -0.018968703715486868 2307 76-77 0.06061627089383137 2307 78-79 0.42928979528681666 2307 80-81 0.2648219065991775 2307 82-83 0.20482815973248236 2307 84-85 0.34480359714808984 2307 86-87 0.3729268227702178 2307 88-89 0.10693673255900649 2307 90-91 0.1169386741070042 2307 92-93 0.3785217394004583 2307 94-95 0.23866227433609666 2307 96-97 0.3419858214028224 2307 98-99 0.24897746885913108 2307 100-101 0.10197310738548282 2201 1 0.14895634974048022 2201 2 0.15835581898395645 2201 3 0.09349881751890621 2201 4 0.1318921899913832 2201 5 0.13540809271175647 2201 6 0.15047270389652567 2201 7 0.20295216743089384 2201 8 0.16258049540233088 2201 9 0.22580343501017808 2201 10-11 0.16576519475692209 2201 12-13 0.15934877134020553 2201 14-15 0.282670035940491 2201 16-17 0.2544695730146245 2201 18-19 0.293249881151354 2201 20-21 0.3669313324395844 2201 22-23 0.33917334304575064 2201 24-25 0.3811940520288246 2201 26-27 0.3883523305263381 2201 28-29 0.4144039066048677 2201 30-31 0.3567746353000132 2201 32-33 0.4686868629229508 2201 34-35 0.35000175109587417 2201 36-37 0.32566028993508667 2201 38-39 0.3797942446667051 2201 40-41 0.3442226822637977 2201 42-43 0.29831562954954194 2201 44-45 0.49988787531273715 2201 46-47 0.3936961766714262 2201 48-49 0.4009976062657188 2201 50-51 0.49559113693078416 2201 52-53 0.38910634710190095 2201 54-55 0.40500394008112295 2201 56-57 0.37169089071741723 2201 58-59 0.417006986131355 2201 60-61 0.44335511459591004 2201 62-63 0.4403877433817698 2201 64-65 0.3042782989841939 2201 66-67 0.4161200879852913 2201 68-69 0.42638230447521863 2201 70-71 0.37678845019539864 2201 72-73 0.18415219011217587 2201 74-75 0.17760138077465015 2201 76-77 0.052940543075067126 2201 78-79 0.5896931465395845 2201 80-81 0.5880075358002834 2201 82-83 0.5139768076682394 2201 84-85 0.6100568262006725 2201 86-87 0.7176098671201565 2201 88-89 0.6819276635311269 2201 90-91 0.4282616482104231 2201 92-93 0.3668112362135183 2201 94-95 0.3287342705019505 2201 96-97 0.5823066073295671 2201 98-99 0.5273846074112676 2201 100-101 0.3548910327340913 1101 1 0.11907093822300396 1101 2 0.13454138625035483 1101 3 0.10966987802748918 1101 4 0.13586167611929767 1101 5 0.08767241378803448 1101 6 0.061901992146296436 1101 7 0.11112737130797257 1101 8 0.0955711976635456 1101 9 0.11168195084961496 1101 10-11 0.1659626754497694 1101 12-13 0.1002610576201377 1101 14-15 0.1771159846054431 1101 16-17 0.34705448350246826 1101 18-19 0.2618839854459978 1101 20-21 0.2688529810431888 1101 22-23 0.16964175731304465 1101 24-25 0.3694246995419306 1101 26-27 0.29667285038494384 1101 28-29 0.26557922723154803 1101 30-31 0.3573074605656146 1101 32-33 0.4737940365643709 1101 34-35 0.3562354340983731 1101 36-37 0.40590452014541256 1101 38-39 0.4034509412494245 1101 40-41 0.34744695823003013 1101 42-43 0.34212901898892056 1101 44-45 0.4186127710454173 1101 46-47 0.4677911810309041 1101 48-49 0.5868269382344948 1101 50-51 0.457002912741757 1101 52-53 0.38870517563152873 1101 54-55 0.43187870254538296 1101 56-57 0.408162718047258 1101 58-59 0.41847629216681526 1101 60-61 0.20179151463466383 1101 62-63 0.36745154082371556 1101 64-65 0.31872047191766484 1101 66-67 0.3765494283997768 1101 68-69 0.25624461447768 1101 70-71 -0.04271767419011496 1101 72-73 -0.18899441011486573 1101 74-75 0.0040444181519063704 1101 76-77 0.0586314646764734 1101 78-79 0.5671157626743764 1101 80-81 0.5869990180481359 1101 82-83 0.479063711593259 1101 84-85 0.41738273889682276 1101 86-87 0.5869211190483874 1101 88-89 0.6379056152464777 1101 90-91 0.5454447041185766 1101 92-93 0.5514097293985714 1101 94-95 0.5198173341106056 1101 96-97 0.6392493578009102 1101 98-99 0.6671438051676901 1101 100-101 0.44003998782524434 2304 1 0.08274625154064807 2304 2 0.09651568376526143 2304 3 0.04787586953460732 2304 4 0.0381881434516842 2304 5 0.07113485979279943 2304 6 0.091221736484556 2304 7 0.11986274389930429 2304 8 0.12680610930900826 2304 9 0.03406602027344263 2304 10-11 0.02602937449623255 2304 12-13 0.14478349617802877 2304 14-15 0.10541002574118608 2304 16-17 0.11489093632133773 2304 18-19 0.1594462095510707 2304 20-21 0.19787251869116318 2304 22-23 0.21112338799245123 2304 24-25 0.17424694661895757 2304 26-27 0.05597032583643369 2304 28-29 0.063595886568514 2304 30-31 0.11385782419272061 2304 32-33 0.04540664043861398 2304 34-35 0.08857699247564454 2304 36-37 0.15304387573949896 2304 38-39 0.1140132557500877 2304 40-41 0.21183282767437817 2304 42-43 0.0351965545535009 2304 44-45 0.17154847609171497 2304 46-47 0.30616302337234913 2304 48-49 0.2173151534449218 2304 50-51 0.1462196670658642 2304 52-53 0.26874159214867177 2304 54-55 0.33132892146776527 2304 56-57 0.3730092284538671 2304 58-59 0.2924730623173275 2304 60-61 0.18737870586370065 2304 62-63 0.24200575056878648 2304 64-65 0.1792741235057278 2304 66-67 0.10045029669983307 2304 68-69 0.09852642566134051 2304 70-71 -0.12121056773207073 2304 72-73 -0.0300717536031172 2304 74-75 -0.2354826944329531 2304 76-77 -0.309700782952973 2304 78-79 0.0894629384931207 2304 80-81 0.03586944957238458 2304 82-83 -0.03491711736318237 2304 84-85 0.08594167504093164 2304 86-87 -0.04837868947075208 2304 88-89 0.016643760687273357 2304 90-91 -0.15638161004347495 2304 92-93 -0.06019091499299378 2304 94-95 -0.13003733342366885 2304 96-97 -0.3473793558623832 2304 98-99 -0.36567814130806653 2304 100-101 -0.1834992442281802 2202 1 0.08133690014867057 2202 2 0.09249688111422216 2202 3 0.10346376527910905 2202 4 0.10219596854609136 2202 5 0.06968232175432831 2202 6 0.06557766876528603 2202 7 0.07733514192830171 2202 8 0.06780402396437069 2202 9 0.09372894279366761 2202 10-11 0.06281250060971644 2202 12-13 0.06993508945564741 2202 14-15 0.0604923280816152 2202 16-17 0.07066650656434348 2202 18-19 0.0419989021488405 2202 20-21 0.12370083857090464 2202 22-23 0.05490938365488773 2202 24-25 0.003002278132697711 2202 26-27 -0.012800578944997199 2202 28-29 0.0976291640797129 2202 30-31 0.16406067105645405 2202 32-33 0.18014237727450677 2202 34-35 0.058913551715669144 2202 36-37 0.15186618403805596 2202 38-39 0.20103459288689152 2202 40-41 0.13239588050419115 2202 42-43 0.2101918610613538 2202 44-45 0.2788770707363142 2202 46-47 0.20028590747112673 2202 48-49 0.20212975072699635 2202 50-51 0.1560053537400563 2202 52-53 0.1150984970252722 2202 54-55 0.07765145629250014 2202 56-57 -0.005122944196237711 2202 58-59 -0.08682889806308225 2202 60-61 -0.0015781100972986906 2202 62-63 0.0698722520071513 2202 64-65 0.039704986084835525 2202 66-67 0.17546264971041126 2202 68-69 0.021797525679005503 2202 70-71 -0.04580546069111335 2202 72-73 0.14305552673330268 2202 74-75 0.23130375949683568 2202 76-77 0.21009442759903862 2202 78-79 0.4719070126007523 2202 80-81 0.4039437781911488 2202 82-83 0.44975537140666333 2202 84-85 0.5603065705879153 2202 86-87 0.6604248350810984 2202 88-89 0.6015851311478677 2202 90-91 0.22609057612649863 2202 92-93 0.3630013082816461 2202 94-95 0.41272535057589366 2202 96-97 0.30390876327930627 2202 98-99 0.190164229713794 2202 100-101 0.16905209247186548 1102 1 0.05453778297116685 1102 2 0.10098391513126614 1102 3 0.06459460030579578 1102 4 0.07700438844675261 1102 5 0.06920840505376447 1102 6 -0.026415377742800672 1102 7 -0.004383797556606339 1102 8 -0.03150129556160408 1102 9 0.04945683668749723 1102 10-11 -0.057995626218534824 1102 12-13 0.059991052330815364 1102 14-15 0.05386345457579722 1102 16-17 0.039697358541545213 1102 18-19 0.05628065812943106 1102 20-21 0.052536285679330774 1102 22-23 -0.012507738344943675 1102 24-25 -0.04387440728808656 1102 26-27 0.011902401758455028 1102 28-29 -0.024563424619032048 1102 30-31 0.10638958785441588 1102 32-33 0.06911968538694424 1102 34-35 -0.012397173949658225 1102 36-37 -0.021079321647057725 1102 38-39 0.08811550733097562 1102 40-41 0.08640800695679474 1102 42-43 -0.032517599217307236 1102 44-45 0.009393134041118856 1102 46-47 0.06485258042760478 1102 48-49 0.09713408721017913 1102 50-51 -0.03148576032180017 1102 52-53 0.05568017307965789 1102 54-55 0.08535943521803091 1102 56-57 0.09314622769683467 1102 58-59 -0.055473129958954814 1102 60-61 -0.06648834358703937 1102 62-63 -0.03900087874762903 1102 64-65 -0.2262158529628735 1102 66-67 0.024457888155929197 1102 68-69 -0.10656797341778912 1102 70-71 -0.21303321953322296 1102 72-73 -0.002420182419072603 1102 74-75 -0.2600711746127544 1102 76-77 -0.016803368920900397 1102 78-79 0.05950375484502146 1102 80-81 -0.14634895986403862 1102 82-83 -0.05852683174194695 1102 84-85 0.12698178035420327 1102 86-87 0.04948856757344089 1102 88-89 0.08301508320668916 1102 90-91 -0.16634174464358154 1102 92-93 -0.0880782041830912 1102 94-95 -0.04151566601322898 1102 96-97 -0.3287921287017035 1102 98-99 -0.22428602836728828 1102 100-101 -0.18430848574139702 2305 1 0.03876054278470775 2305 2 -0.015679962368530198 2305 3 0.02642238019604548 2305 4 0.009707175883640673 2305 5 0.023353707231756005 2305 6 0.02582112807366599 2305 7 0.05974832845583222 2305 8 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-0.6679664300092654 1213 58-59 -0.5369930899785444 1213 60-61 -0.5798877258434629 1213 62-63 -0.47417516323315567 1213 64-65 -0.43886446583486816 1213 66-67 -0.24573207097669325 1213 68-69 -0.31060139999403447 1213 70-71 -0.3535869340817257 1213 72-73 0.22078720218071624 1213 74-75 0.17792946564030743 1213 76-77 0.016686748150753772 1213 78-79 -0.690703078943347 1213 80-81 -0.7582711016633219 1213 82-83 -0.47915455179260036 1213 84-85 -0.4902517972293374 1213 86-87 -0.587590898340725 1213 88-89 -0.4563659769301438 1213 90-91 -0.2891347722206774 1213 92-93 -0.1647121724775218 1213 94-95 -0.21751212039761825 1213 96-97 -0.22690934494996995 1213 98-99 -0.22908746047698259 1213 100-101 0.09406901685427016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 11.0 10 24.0 11 25.0 12 42.0 13 112.0 14 257.0 15 752.0 16 1559.0 17 2622.0 18 3620.0 19 4892.0 20 6589.0 21 9002.0 22 12365.0 23 16842.0 24 22737.0 25 30467.0 26 42520.0 27 65252.0 28 115910.0 29 189430.0 30 143896.0 31 76315.0 32 89721.0 33 127307.0 34 195953.0 35 333295.0 36 575126.0 37 680585.0 38 105686.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.34106104276163 19.701464350916286 19.68974027665305 12.267734329669032 2 28.9083689749038 31.493489878167868 28.531564959960633 11.066576186967698 3 36.87053574871133 14.133620662460014 35.23149086101295 13.764352727815707 4 29.201235394544494 13.663344469646244 28.13951124089843 28.995908894910833 5 44.15635945949072 12.209058798663413 29.137180991251476 14.497400750594386 6 38.80786921009961 13.112534359268432 33.36601304898593 14.713583381646034 7 31.844601117022357 28.86269682313906 23.018449025602344 16.27425303423624 8 27.61451161642964 37.97732305809231 18.665258529616093 15.742906795861956 9 42.94833193244026 21.264715511216142 18.963582154842264 16.82337040150133 10-11 31.262044584152076 30.867171502259772 22.903462460856197 14.967321452731952 12-13 23.93553742066961 29.260742069064143 22.481161551829658 24.322558958436588 14-15 23.095071683896833 29.604404856460455 22.697131592580572 24.60339186706214 16-17 29.5943100140136 22.910525345415365 23.68802099727229 23.80714364329874 18-19 20.911606334794048 23.082803646201008 31.30957446733933 24.696015551665614 20-21 27.51815494321301 23.969011637811075 31.35054971324338 17.16228370573254 22-23 20.123094459697764 38.27192105241942 24.03892698421378 17.566057503669033 24-25 20.541339431365937 22.036410133819757 32.405495800475435 25.016754634338866 26-27 19.076903771615406 22.1845554518809 25.347115363719276 33.391425412784415 28-29 26.113930231245742 30.746205930095243 23.656622171031348 19.483241667627663 30-31 17.259482423209985 24.47611386268757 31.32198351858278 26.942420195519663 32-33 17.74863407132185 30.770992801216302 23.994076229116736 27.486296898345113 34-35 18.836489847651684 30.386924207202686 31.65414506319104 19.12244088195459 36-37 17.648824827780565 32.37215381816614 32.181751136825284 17.797270217228007 38-39 17.58946093746029 25.007838956203326 30.222545731918387 27.18015437441799 40-41 26.344220689336034 23.91683992121293 22.589109161670024 27.149830227781013 42-43 19.445410365202175 31.44943731999989 30.43416307484359 18.67098923995435 44-45 17.88601029731179 25.13168707907674 30.16882410602197 26.813478517589502 46-47 26.169824065022297 24.29560988023186 23.72777240513171 25.806793649614136 48-49 18.665152581632995 30.979000044868705 31.610895102581072 18.744952270917228 50-51 25.327705772164393 23.78600942771895 29.877379363334526 21.008905436782126 52-53 17.3483679849768 24.498357582385108 23.205964830301042 34.94730960233705 54-55 25.737408754069936 24.39387039107477 31.38917841938437 18.47954243547093 56-57 18.856019997125237 24.208686689501157 30.128610543365085 26.806682770008518 58-59 17.92594236424698 23.94254900280061 30.39490943371289 27.73659919923952 60-61 24.5202543310732 23.592062663148763 31.927609148724358 19.960073857053683 62-63 17.614912153553774 23.36450285314068 31.820971009033965 27.199613984271576 64-65 26.16583448591842 25.007243276460393 30.336787378592962 18.490134859028228 66-67 19.274825854598777 38.79317039734165 22.989471949595785 18.942531798463786 68-69 18.43675776474398 37.29393177997252 24.66073816460491 19.60857229067859 70-71 18.018302801488925 39.5016618110611 23.569454795881352 18.91058059156862 72-73 19.776553353891032 39.07674426350786 23.09054445178787 18.05615793081323 74-75 21.075157967081132 36.767968974634094 23.32025414992321 18.83661890836156 76-77 20.469119825305494 34.904943419056615 23.832437870882654 20.793498884755234 78-79 20.300278098246892 34.73353504483121 24.37487489435036 20.591311962571545 80-81 19.555421021967614 34.95786730532339 24.50563580441231 20.98107586829668 82-83 19.237601890624482 34.084766830224474 24.709549939112748 21.968081340038292 84-85 20.05277373196693 32.9624274164007 24.668573601916748 22.316225249715625 86-87 18.91359505854792 33.239490246220655 25.44849224076607 22.398422454465358 88-89 18.543287010539945 33.50814305239611 25.58792528028308 22.360644656780863 90-91 19.40179627986825 32.47815561507046 24.85992582313134 23.260122281929945 92-93 19.932035376386008 32.22367808386324 24.1102608910294 23.734025648721353 94-95 20.28830392440459 33.3031267257475 23.596409568639825 22.812159781208088 96-97 19.785420682125064 34.082515456702474 22.88917352355313 23.242890337619333 98-99 19.775463996897923 32.30609736344443 24.53538955825546 23.38304908140219 100-101 20.752387827418005 33.21394926653388 23.778669618224583 22.254993287823527 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1456.0 1 870.5 2 385.5 3 631.5 4 601.5 5 356.0 6 313.0 7 380.0 8 413.5 9 398.5 10 380.0 11 350.0 12 319.0 13 300.0 14 301.5 15 332.5 16 331.5 17 338.0 18 368.5 19 362.0 20 367.0 21 394.0 22 451.0 23 504.5 24 576.0 25 703.0 26 872.0 27 1145.0 28 1454.0 29 1890.5 30 2529.5 31 3599.5 32 6022.0 33 10752.5 34 18977.0 35 33428.0 36 54628.5 37 83209.0 38 120671.0 39 152301.5 40 170496.5 41 173503.0 42 188210.5 43 218058.0 44 208913.5 45 179589.5 46 172927.5 47 166818.5 48 144549.5 49 119866.5 50 97979.0 51 84598.5 52 80231.0 53 81142.0 54 72335.5 55 46886.0 56 28323.5 57 23260.0 58 22268.0 59 19491.0 60 15180.5 61 11394.5 62 7671.5 63 4860.0 64 3321.5 65 2246.5 66 1553.5 67 1198.0 68 883.0 69 656.0 70 470.5 71 333.0 72 242.5 73 173.5 74 117.0 75 67.5 76 45.5 77 40.5 78 29.0 79 19.0 80 15.5 81 10.5 82 9.5 83 7.5 84 3.0 85 2.5 86 2.0 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14381645333133306 2 0.0 3 0.0 4 0.014441232944798738 5 1.0515460882135003E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.855668990116168E-4 24-25 0.0 26-27 0.0 28-29 1.0515460882135003E-4 30-31 0.0011216491607610672 32-33 0.0010340203200766087 34-35 2.1030921764270005E-4 36-37 0.0014371129872251171 38-39 0.007097936095441127 40-41 0.015545356337422914 42-43 0.005818555021448035 44-45 0.0021556694808376755 46-47 4.5566997155918344E-4 48-49 0.006098967311638302 50-51 0.002734019829355101 52-53 0.01007731667871271 54-55 0.021837107098567024 56-57 0.01864741729765274 58-59 0.018524736920694496 60-61 0.02367731275294065 62-63 0.007308245313083828 64-65 0.01039278050517676 66-67 0.02802370325088978 68-69 0.03945050407614315 70-71 0.03797833955264425 72-73 0.02828658977294316 74-75 0.03792576224823358 76-77 0.030757723080244884 78-79 0.012373192304645522 80-81 0.010094842446849604 82-83 0.004486596643044269 84-85 0.006905152645935319 86-87 0.023239168549518355 88-89 0.021118550604954465 90-91 0.015264944047232646 92-93 0.030494836558191508 94-95 0.032142258763059324 96-97 0.033053598706177696 98-99 0.023134013940697005 100-101 0.023239168549518355 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2852942.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.85936937982591 #Duplication Level Percentage of deduplicated Percentage of total 1 76.55605007760502 19.031351271396332 2 9.228422432691028 4.588255240946758 3 4.328132029674866 3.2278389855102945 4 1.9327026448295277 1.9218307579673484 5 1.286518225524951 1.5991015891101468 6 1.0858628408398705 1.619631927375927 7 0.6945333714235346 1.2085963138783427 8 0.5828704837181123 1.1591834124277087 9 0.4959275354150259 1.1095601209657941 >10 3.072730131488479 15.213774738304306 >50 0.3794426289003175 6.6108704588110525 >100 0.31484305606343294 16.109635227863283 >500 0.029027974932421898 4.7794899378221 >1k 0.011661407479769252 5.312120335969143 >5k 5.667375171548091E-4 1.0079082650138458 >10k+ 7.084218963827537E-4 15.500851416637559 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 378801 13.277556992045406 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20092 0.7042554668128549 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 18952 0.664296715460742 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11136 0.39033390794485134 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 11111 0.3894576195380067 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8233 0.2885792981420583 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7591 0.26607621185428937 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7532 0.2640081712141361 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5260 0.1843710808001004 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4983 0.1746618052522624 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 4859 0.17031541475431325 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 3982 0.13957521744220527 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3864 0.13543913616189884 TruSeq Adapter, Index 27 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3840 0.13459789929132804 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3688 0.12927006577771297 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3604 0.12632573673071518 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3580 0.12548449986014437 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3446 0.1207875939994574 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3251 0.11395254442606965 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.006519585746923702 0.0 0.0 2 0.0070804103273042355 0.0 0.0 3 0.0075711318351372025 0.0 0.0 4 0.008096904879243952 0.0 0.0 5 0.009779378620385554 0.0 0.0 6 0.010830924708599053 0.0 0.0 7 0.012197934623276603 0.0 0.0 8 0.013284532247763887 0.0 0.0 9 0.015422675960464672 0.0 0.0 10-11 0.024536075391648343 0.0 0.0 12-13 0.032948444097356346 0.0 0.0 14-15 0.041027823208463404 0.0 0.0 16-17 0.04980823304504613 0.0 0.0 18-19 0.05971029204238992 0.0 0.0 20-21 0.0658618366584389 0.0 0.0 22-23 0.07118967017205396 0.0 0.0 24-25 0.07718348287487092 0.0 0.0 26-27 0.08358038824483638 0.0 0.0 28-29 0.09094121086233088 0.0 0.0 30-31 0.1708587135665569 0.0 0.0 32-33 0.1847741734672489 0.0 0.0 34-35 0.19409788211607526 0.0 0.0 36-37 0.20591024984034026 0.0 0.0 38-39 0.21777519486901592 0.0 0.0 40-41 0.23156797439274968 0.0 0.0 42-43 0.24732363994781528 0.0 0.0 44-45 0.2648669338528438 0.0 0.0 46-47 0.2829710523382529 0.0 0.0 48-49 0.30338857221773174 0.0 0.0 50-51 0.32622464810010154 0.0 0.0 52-53 0.3505504142741072 0.0 0.0 54-55 0.3771370045377719 0.0 0.0 56-57 0.406405037326381 0.0 0.0 58-59 0.43819678072670243 0.0 0.0 60-61 0.4732307912323489 0.0 0.0 62-63 0.5104730485232437 0.0 0.0 64-65 0.550869944078779 0.0 0.0 66-67 0.5946668386528713 0.0 0.0 68-69 0.6417761034048362 0.0 0.0 70-71 0.6943358820473742 0.0 0.0 72-73 0.7526090611025391 0.0 0.0 74-75 0.8151935791193792 0.0 0.0 76-77 0.8852966516669458 0.0 0.0 78-79 0.9668615765760398 0.0 0.0 80-81 1.0581007254966979 0.0 0.0 82-83 1.1606615206337878 0.0 0.0 84-85 1.28052024892199 0.0 0.0 86-87 1.414662478241759 0.0 0.0 88-89 1.5577253235432056 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 48755 0.0 83.0152 3 CGGAAGA 48660 0.0 83.011314 4 GAAGAGC 48740 0.0 82.88251 6 GATCGGA 49070 0.0 82.69218 1 ATCGGAA 49685 0.0 81.49958 2 GGAAGAG 50935 0.0 79.413315 5 CGACGCG 20 0.0020623435 71.35472 1 GAGCACA 56935 0.0 70.927704 9 AGAGCAC 57000 0.0 70.855156 8 AAGAGCA 57220 0.0 70.491425 7 ATCGGAC 135 0.0 59.820816 2 GTTCGGC 25 0.004992927 57.08378 1 CGAGGTG 375 0.0 57.083775 1 GATTCGC 35 2.7908848E-4 54.27689 7 TTGTGTT 23955 0.0 51.710205 1 GGTCCGC 60 1.478038E-6 47.569817 1 ATGATCG 80 4.1745807E-9 47.569813 1 GCCGTCT 44060 0.0 45.873493 50-51 ATGCCGT 43980 0.0 45.831123 48-49 CTGCTTG 44265 0.0 45.812885 58-59 >>END_MODULE