FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418366
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1979955.79209481957169No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1186633.4713368901984163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT40590.11874094230986383TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG1800.060.762031
CGACTGG652.5711197E-643.894981
AGTCGTG554.9328733E-543.2299041
ACGCGGC704.3174623E-640.7077452
CGCGGGG2050.039.434111
CGTGCGG756.8735935E-638.0423161
ACCCGCG905.6528734E-736.9855841
CGGACCC905.6528734E-736.9855841
GCGGGCT2300.035.1030584
CGATGCG550.002566290634.5839231
GTCGCGC702.0407909E-433.9663541
CGACGGG1404.5110937E-1033.9663541
AATGCGC702.0407909E-433.9663541
CCGTTGT2500.032.2891437
CATGCGG1351.1857992E-831.701931
GGACGGG3850.030.8785021
TTGACGT3550.030.7643055
ATCGAGC1252.1350934E-730.4338511
CTGCGGA1102.711904E-630.2609311
GTGTTTG456650.030.0947911
Produced by FastQC (version 0.11.2)