##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6004709 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67939711982712 33.0 33.0 33.0 33.0 33.0 2 32.70915526464313 33.0 33.0 33.0 33.0 33.0 3 32.73528725538573 33.0 33.0 33.0 33.0 33.0 4 36.66122421586125 37.0 37.0 37.0 37.0 37.0 5 36.65488052793233 37.0 37.0 37.0 37.0 37.0 6 36.51638872091887 37.0 37.0 37.0 37.0 37.0 7 36.46281726558273 37.0 37.0 37.0 37.0 37.0 8 36.37409739589379 37.0 37.0 37.0 37.0 37.0 9 36.36945820355324 37.0 37.0 37.0 37.0 37.0 10-11 36.32068931233803 37.0 37.0 37.0 37.0 37.0 12-13 36.32554158744412 37.0 37.0 37.0 37.0 37.0 14-15 38.1593430256154 40.0 37.0 40.0 37.0 40.0 16-17 38.128237604853126 40.0 37.0 40.0 37.0 40.0 18-19 38.096876384850624 40.0 37.0 40.0 37.0 40.0 20-21 38.016566914400016 40.0 37.0 40.0 33.0 40.0 22-23 37.916154471432336 40.0 37.0 40.0 33.0 40.0 24-25 37.89485110768898 40.0 37.0 40.0 33.0 40.0 26-27 37.80711846319281 40.0 37.0 40.0 33.0 40.0 28-29 37.68025761115152 40.0 37.0 40.0 33.0 40.0 30-31 37.64213095089204 40.0 37.0 40.0 33.0 40.0 32-33 37.516607549175156 40.0 37.0 40.0 33.0 40.0 34-35 37.423448413570085 40.0 37.0 40.0 33.0 40.0 36-37 37.30502668822086 40.0 37.0 40.0 33.0 40.0 38-39 37.22683255758106 40.0 37.0 40.0 33.0 40.0 40-41 37.106418812302145 40.0 37.0 40.0 33.0 40.0 42-43 36.97950791620377 40.0 37.0 40.0 33.0 40.0 44-45 36.82414768142802 37.0 37.0 40.0 33.0 40.0 46-47 36.71873957588952 37.0 37.0 40.0 33.0 40.0 48-49 36.527902018232695 37.0 37.0 40.0 33.0 40.0 50-51 36.371405675112655 37.0 37.0 40.0 33.0 40.0 52-53 36.23043631256735 37.0 37.0 40.0 33.0 40.0 54-55 36.04128568095473 37.0 37.0 40.0 33.0 40.0 56-57 35.842533584891456 37.0 37.0 40.0 27.0 40.0 58-59 35.61137200820223 37.0 37.0 40.0 27.0 40.0 60-61 35.323199758722694 37.0 33.0 40.0 27.0 40.0 62-63 35.01434923823952 37.0 33.0 38.5 27.0 40.0 64-65 34.74443007979238 37.0 33.0 37.0 27.0 40.0 66-67 34.425830294190774 37.0 33.0 37.0 27.0 40.0 68-69 34.16348044509734 37.0 33.0 37.0 27.0 40.0 70-71 33.88026022576614 37.0 33.0 37.0 27.0 38.5 72-73 33.63056103135057 37.0 33.0 37.0 27.0 37.0 74-75 33.27887221512316 37.0 33.0 37.0 27.0 37.0 76-77 30.508388832831034 33.0 30.0 35.0 24.5 37.0 78-79 33.01197360271747 35.0 33.0 37.0 27.0 37.0 80-81 33.434496659205294 37.0 33.0 37.0 27.0 37.0 82-83 33.38851957688541 37.0 33.0 37.0 27.0 37.0 84-85 33.26946559775003 37.0 33.0 37.0 27.0 37.0 86-87 33.097242264362855 37.0 33.0 37.0 27.0 37.0 88-89 32.994223367027445 37.0 33.0 37.0 27.0 37.0 90-91 32.873184778812764 37.0 33.0 37.0 27.0 37.0 92-93 32.68318697875284 33.0 33.0 37.0 27.0 37.0 94-95 32.57225762314211 33.0 33.0 37.0 27.0 37.0 96-97 32.40998756142887 33.0 33.0 37.0 22.0 37.0 98-99 32.312813660079115 33.0 33.0 37.0 22.0 37.0 100-101 30.66003648136821 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14913508102327455 2306 2 0.14885556143359224 2306 3 0.1101094330696526 2306 4 0.1664397344105879 2306 5 0.16760754742136186 2306 6 0.16993719752614567 2306 7 0.2500958040089287 2306 8 0.28252338457727433 2306 9 0.2580831189466579 2306 10-11 0.2283466486473884 2306 12-13 0.19224869432080993 2306 14-15 0.4122474086884438 2306 16-17 0.43433824422493217 2306 18-19 0.503940849189469 2306 20-21 0.4332916797030535 2306 22-23 0.4185771735127659 2306 24-25 0.39463212347288845 2306 26-27 0.4526758226940757 2306 28-29 0.45533846395823474 2306 30-31 0.4991912756142156 2306 32-33 0.52214422390648 2306 34-35 0.5260623943761331 2306 36-37 0.49614652994208086 2306 38-39 0.5541954258251636 2306 40-41 0.4813319694417615 2306 42-43 0.5208641232045608 2306 44-45 0.6117051368547308 2306 46-47 0.6328889287662136 2306 48-49 0.5604088133803913 2306 50-51 0.5376077543352835 2306 52-53 0.6261518842844609 2306 54-55 0.6184311314105386 2306 56-57 0.714428810032004 2306 58-59 0.5587383215668567 2306 60-61 0.5252503930668198 2306 62-63 0.5428944036142838 2306 64-65 0.3854769365570121 2306 66-67 0.49985885371510363 2306 68-69 0.5349914977912675 2306 70-71 0.5102875849312412 2306 72-73 0.5569733132012331 2306 74-75 0.48545343998480917 2306 76-77 -0.04463014018621081 2306 78-79 0.35358337990459177 2306 80-81 0.4721139172934343 2306 82-83 0.4563072583746859 2306 84-85 0.5199391490908951 2306 86-87 0.5338407766321325 2306 88-89 0.5973291500968401 2306 90-91 0.561021107570248 2306 92-93 0.40638099525698124 2306 94-95 0.429914620848038 2306 96-97 0.4273586373025111 2306 98-99 0.42400683877845324 2306 100-101 0.38235081825362727 2307 1 0.14236527268467114 2307 2 0.11575582221215797 2307 3 0.14010943032374712 2307 4 0.16979358239785114 2307 5 0.0986717200377143 2307 6 0.15782792260149847 2307 7 0.14680744584849492 2307 8 0.19770650758803754 2307 9 0.11806043228212104 2307 10-11 0.16035593419868377 2307 12-13 0.18892334882421835 2307 14-15 0.3634275573352497 2307 16-17 0.37231023434955546 2307 18-19 0.41012355564649283 2307 20-21 0.33250305587633733 2307 22-23 0.3119443309000616 2307 24-25 0.3403703009116015 2307 26-27 0.32233904022986337 2307 28-29 0.3160434420363103 2307 30-31 0.27093583398625043 2307 32-33 0.28315786913064755 2307 34-35 0.30872628987578565 2307 36-37 0.2536761163786352 2307 38-39 0.2813182916050678 2307 40-41 0.36541791406181545 2307 42-43 0.259467671407684 2307 44-45 0.37666547088854685 2307 46-47 0.429415167121185 2307 48-49 0.3369846262986016 2307 50-51 0.28205711394912214 2307 52-53 0.31184981225224107 2307 54-55 0.3915851626594815 2307 56-57 0.32842877763032874 2307 58-59 0.32431988907753606 2307 60-61 0.32944218132900716 2307 62-63 0.22928144382284188 2307 64-65 0.22501359809158572 2307 66-67 0.22045028314247617 2307 68-69 0.24188237306760385 2307 70-71 0.13896770817843418 2307 72-73 0.2680976046548551 2307 74-75 0.2942303124378114 2307 76-77 0.1859894704609779 2307 78-79 0.4517507356560415 2307 80-81 0.41810021467985337 2307 82-83 0.41591000285151125 2307 84-85 0.40654470839461254 2307 86-87 0.359848283386377 2307 88-89 0.2944091550504524 2307 90-91 0.24457860283287403 2307 92-93 0.33685562213658216 2307 94-95 0.2385560652817844 2307 96-97 0.2963007253488499 2307 98-99 0.2835188036462455 2307 100-101 0.09890200589859077 2201 1 0.14255443479690655 2201 2 0.15892330259689658 2201 3 0.11946719806125827 2201 4 0.1643761598041067 2201 5 0.14608108267613318 2201 6 0.20178339917759303 2201 7 0.17505826692116955 2201 8 0.20597621618772166 2201 9 0.16810418338370425 2201 10-11 0.1738009399373155 2201 12-13 0.16441123264005597 2201 14-15 0.2998405058583131 2201 16-17 0.30195724391579404 2201 18-19 0.3786537260813958 2201 20-21 0.43666225589834795 2201 22-23 0.36143247912284693 2201 24-25 0.4078199628144219 2201 26-27 0.43836031305372813 2201 28-29 0.46403300938470693 2201 30-31 0.373753791140949 2201 32-33 0.48482819158265045 2201 34-35 0.48113195814178766 2201 36-37 0.49289401684104206 2201 38-39 0.4426414316633469 2201 40-41 0.361761240888093 2201 42-43 0.3798027162833577 2201 44-45 0.375615347128992 2201 46-47 0.4631429561316125 2201 48-49 0.4771538026884059 2201 50-51 0.4004739023657393 2201 52-53 0.32462545149135025 2201 54-55 0.42991456463419553 2201 56-57 0.39632803889414703 2201 58-59 0.41465368465348007 2201 60-61 0.44399372139698556 2201 62-63 0.45731644695786855 2201 64-65 0.4889389490774505 2201 66-67 0.5010719693160226 2201 68-69 0.4468600872301849 2201 70-71 0.3420397408341742 2201 72-73 0.36340439686546944 2201 74-75 0.27362116945896275 2201 76-77 0.04683574067124496 2201 78-79 0.48668825555891715 2201 80-81 0.37310619047130444 2201 82-83 0.4498910324476242 2201 84-85 0.48349447778638677 2201 86-87 0.5957727244681621 2201 88-89 0.41114673915473077 2201 90-91 0.32997333621750613 2201 92-93 0.27948095560972064 2201 94-95 0.35644598625012236 2201 96-97 0.45659522919321915 2201 98-99 0.4401262853996357 2201 100-101 0.40992672518135365 1101 1 0.10128584579515376 1101 2 0.07711827772525481 1101 3 0.07325092608972028 1101 4 0.0661721664624082 1101 5 0.09434753406567609 1101 6 0.045260527724984456 1101 7 0.04375230854486034 1101 8 0.17497151773434894 1101 9 0.15261682841965296 1101 10-11 0.15355209449554508 1101 12-13 0.16732103760730865 1101 14-15 0.2509718500438183 1101 16-17 0.2185444668832588 1101 18-19 0.2500020581968272 1101 20-21 0.3129049554343126 1101 22-23 0.2789918811186922 1101 24-25 0.2318222511695751 1101 26-27 0.2575526914676729 1101 28-29 0.3307744543457787 1101 30-31 0.36033032463502224 1101 32-33 0.33847995126882324 1101 34-35 0.33985554481837 1101 36-37 0.2867425841098239 1101 38-39 0.25449683725744165 1101 40-41 0.30009715926055236 1101 42-43 0.38413031469962533 1101 44-45 0.3780149705446689 1101 46-47 0.4381262647161748 1101 48-49 0.4804239407363724 1101 50-51 0.5044070692886535 1101 52-53 0.47039190499939565 1101 54-55 0.48299427790058047 1101 56-57 0.22583133852640458 1101 58-59 0.3583905740892206 1101 60-61 0.172760739178905 1101 62-63 0.3449620360695036 1101 64-65 0.3067117921657143 1101 66-67 0.3332183078583171 1101 68-69 0.30994368535231587 1101 70-71 0.21103872856844674 1101 72-73 0.18120774633423054 1101 74-75 0.11397259448826702 1101 76-77 -0.032096694192688346 1101 78-79 0.37043947922726517 1101 80-81 0.33949538560093373 1101 82-83 0.3403111015408058 1101 84-85 0.3651270144983201 1101 86-87 0.44622453902815806 1101 88-89 0.48107428367639216 1101 90-91 0.2722641855556276 1101 92-93 0.2538988369058188 1101 94-95 0.23660336527750303 1101 96-97 0.20696260039721892 1101 98-99 0.21874837904311306 1101 100-101 0.2575144708059689 2304 1 0.13226025873652958 2304 2 0.11773537180477689 2304 3 0.11242777027283068 2304 4 0.06677571606994093 2304 5 0.0562936997491974 2304 6 0.13397555681358142 2304 7 0.1293492234351703 2304 8 0.19319816580667748 2304 9 0.13406342672371352 2304 10-11 0.15457004903679916 2304 12-13 0.1451819353388757 2304 14-15 0.26486009642696473 2304 16-17 0.2655221874892959 2304 18-19 0.3626574375029179 2304 20-21 0.34991786281113946 2304 22-23 0.3194994336046548 2304 24-25 0.31896433601730934 2304 26-27 0.24703018325148918 2304 28-29 0.3753750840465031 2304 30-31 0.36560586040591403 2304 32-33 0.44163116014901505 2304 34-35 0.377731145786683 2304 36-37 0.4829714586336351 2304 38-39 0.44222797588133034 2304 40-41 0.3897978147065899 2304 42-43 0.39446999057914667 2304 44-45 0.5352900292832246 2304 46-47 0.4748722278638482 2304 48-49 0.5803232815029276 2304 50-51 0.5360706541821472 2304 52-53 0.4969184197461445 2304 54-55 0.48662826785224667 2304 56-57 0.5186475156036892 2304 58-59 0.4945211970853407 2304 60-61 0.40929744835014503 2304 62-63 0.4608535477344802 2304 64-65 0.411531644826745 2304 66-67 0.43067838119866053 2304 68-69 0.42880165731561704 2304 70-71 0.35067360044377693 2304 72-73 0.45201889067529066 2304 74-75 0.4022721590722611 2304 76-77 0.1894660691501251 2304 78-79 0.5876308814633688 2304 80-81 0.466992715256886 2304 82-83 0.4728234027078244 2304 84-85 0.6316033867440538 2304 86-87 0.6436546287670524 2304 88-89 0.6281035400064212 2304 90-91 0.47072823611924974 2304 92-93 0.4976676583789441 2304 94-95 0.5514785230295658 2304 96-97 0.43077187944264494 2304 98-99 0.405348572290805 2304 100-101 0.43697671829557905 2202 1 0.10554624748936448 2202 2 0.051164383653940604 2202 3 0.06234249974254169 2202 4 0.07344633879316831 2202 5 0.09533037067649275 2202 6 0.08710188740895575 2202 7 0.15165839804546266 2202 8 0.10502702704496159 2202 9 0.150679395769167 2202 10-11 0.10764619644820783 2202 12-13 0.13424216476698803 2202 14-15 0.19595324970317307 2202 16-17 0.18087425574169913 2202 18-19 0.22161805893222208 2202 20-21 0.2373644013672589 2202 22-23 0.18619423419752934 2202 24-25 0.19265176980054832 2202 26-27 0.09750477717141592 2202 28-29 0.17768167079891128 2202 30-31 0.19160185351886128 2202 32-33 0.20768835834929433 2202 34-35 0.21747191438047508 2202 36-37 0.26427320429299783 2202 38-39 0.12979859261528048 2202 40-41 0.18545391333299932 2202 42-43 0.23902380688253544 2202 44-45 0.36729105870283973 2202 46-47 0.4882440942510655 2202 48-49 0.3542015953561304 2202 50-51 0.3868931167067515 2202 52-53 0.3340341683330905 2202 54-55 0.393654158900155 2202 56-57 0.40999370659782386 2202 58-59 0.34745769011274064 2202 60-61 0.2962007207230002 2202 62-63 0.31150025668856784 2202 64-65 0.3682061820427407 2202 66-67 0.32210574425938177 2202 68-69 0.32165559236202057 2202 70-71 0.06894684103259152 2202 72-73 0.20450708740645496 2202 74-75 0.22966962235830835 2202 76-77 0.27950600515276847 2202 78-79 0.43545236914641094 2202 80-81 0.39835005080168884 2202 82-83 0.33143836318371456 2202 84-85 0.41046136465404714 2202 86-87 0.4149536291711726 2202 88-89 0.5010496731110337 2202 90-91 0.3359665592282326 2202 92-93 0.42320245678870094 2202 94-95 0.4863206156622084 2202 96-97 0.3631758052189582 2202 98-99 0.3264098720209603 2202 100-101 0.387882151713729 1102 1 0.09601565004960122 1102 2 0.09803505687293068 1102 3 0.05308726078472148 1102 4 0.05795550573144226 1102 5 -0.022993892868328203 1102 6 0.04065538239630939 1102 7 0.03336448407974757 1102 8 0.07849140142658229 1102 9 0.06960892947346764 1102 10-11 -0.024923305729849687 1102 12-13 0.08149665300481956 1102 14-15 0.09695812495862555 1102 16-17 0.12472020625577329 1102 18-19 0.1401957725725964 1102 20-21 0.05981678839103921 1102 22-23 0.10475547679640584 1102 24-25 0.012592469903118797 1102 26-27 0.045970189418767404 1102 28-29 0.001029076589141198 1102 30-31 0.09355567162207734 1102 32-33 0.11731757809445043 1102 34-35 0.022065406137919297 1102 36-37 -0.004671872336409422 1102 38-39 0.009774009456215538 1102 40-41 0.07037810903717912 1102 42-43 0.1333404887015206 1102 44-45 0.03514694147962416 1102 46-47 0.06762490476527461 1102 48-49 -0.008671920076750439 1102 50-51 0.02629391853710672 1102 52-53 -0.02480646530113262 1102 54-55 -0.06289322873562497 1102 56-57 -0.0223996159278812 1102 58-59 -0.04874133966168159 1102 60-61 -0.05617203045701302 1102 62-63 -0.09788165546695637 1102 64-65 -0.11228306561643109 1102 66-67 0.011225084853592193 1102 68-69 -0.04454042423152771 1102 70-71 -0.12935265960621933 1102 72-73 -0.04976220482372895 1102 74-75 -0.109287232189196 1102 76-77 -0.03887506188105405 1102 78-79 0.09106506579258422 1102 80-81 0.0014522029567061168 1102 82-83 -0.08295975022008406 1102 84-85 -0.008855195317259756 1102 86-87 0.08249554244113 1102 88-89 0.03094380112770523 1102 90-91 -0.13383612806453726 1102 92-93 -0.12327813100867502 1102 94-95 -0.2231601123723479 1102 96-97 -0.2951110073600489 1102 98-99 -0.27574748529254833 1102 100-101 -0.293496611690248 2305 1 0.01686676425088507 2305 2 -0.01072192379960768 2305 3 0.011507698712719616 2305 4 -0.0018097385117883391 2305 5 0.02230282701866315 2305 6 -0.029082849187602733 2305 7 -0.0016581946247811175 2305 8 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-0.3263039916784223 1213 92-93 -0.27730173098013466 1213 94-95 -0.45177124179409134 1213 96-97 -0.28334783833600596 1213 98-99 -0.24389726960158242 1213 100-101 -0.023632131732238548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 8.0 8 53.0 9 220.0 10 322.0 11 220.0 12 267.0 13 549.0 14 1345.0 15 2974.0 16 5250.0 17 8030.0 18 10614.0 19 12969.0 20 15893.0 21 19609.0 22 23516.0 23 28623.0 24 34856.0 25 42315.0 26 51725.0 27 63614.0 28 80246.0 29 101029.0 30 119271.0 31 146312.0 32 192581.0 33 268104.0 34 401728.0 35 679707.0 36 1299041.0 37 1934570.0 38 458946.0 39 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.156140433391336 28.96167672540463 16.422878617001086 16.45930422420295 2 33.398521060720846 23.431859895292177 28.446141186858515 14.723477857128465 3 39.16742676456095 20.59521951854786 21.880360896756194 18.356992820134998 4 33.26856253125138 20.629328347991432 27.247026600445658 18.855082520311527 5 35.144826649009374 18.51452596434524 26.94695951302114 19.393687873624245 6 32.17329932224859 19.52552571656678 28.695212374155016 19.605962587029612 7 35.92015866214332 20.0468498973056 22.252602082798685 21.78038935775239 8 31.07964099509235 26.014799384949377 22.171848794004838 20.73371082595343 9 29.43879545203606 22.541825090941124 26.885865743036007 21.133513713986808 10-11 22.58622357886119 30.127788041019137 31.461582901019852 15.82440547909982 12-13 22.48168895445225 29.31981383277691 31.574161212475076 16.624336000295767 14-15 22.37972731068233 28.999931553718923 31.854624428927362 16.76571670667138 16-17 22.119398292240305 28.90343228955808 31.87251871822598 17.104650699975636 18-19 21.02215944186471 29.267704729737943 31.94337977077657 17.76675605762078 20-21 21.622163538649417 29.052240499914316 31.233328709184743 18.092267252251524 22-23 20.95284780932652 28.587182911636887 31.52659140277661 18.933377876259982 24-25 20.497970842550405 27.76846471660825 32.22372641205427 19.509838028787073 26-27 20.514632765717707 28.216696262883012 31.658703194442893 19.609967776956385 28-29 20.669241869500667 28.78480291704588 31.000956749748738 19.54499846370471 30-31 19.756906507953968 30.450661983403016 30.52934274460378 19.26308876403924 32-33 19.395222187332603 31.140108630783985 31.06349302703079 18.401176154852617 34-35 19.991098639399137 30.32137325761487 32.191574932969175 17.49595317001682 36-37 19.667164339428922 29.95872341523978 33.15172579757403 17.22238644775727 38-39 20.15492165937837 29.413763776571656 31.794131850208696 18.63718271384128 40-41 20.3704221896538 29.145665282703153 31.377104489828145 19.1068080378149 42-43 20.378621907788823 28.792337527576738 31.43024496865247 19.398795595981966 44-45 19.986501992642296 28.318111644591887 31.844797063579694 19.850589299186122 46-47 20.722466656624498 28.620766290818068 31.01210997391451 19.644657078642926 48-49 20.472955204332592 29.777179989251128 30.45175725011504 19.298107556301236 50-51 21.435718964891137 29.58477518735116 28.928266511828955 20.05123933592875 52-53 21.058646777334015 29.53539416759194 28.797397174042793 20.608561881031253 54-55 21.70649345349317 29.37170941263627 29.134035464114667 19.787761669755895 56-57 21.18782057416208 30.094551029397692 29.015537137111526 19.7020912593287 58-59 20.78549138892889 31.032203371338717 28.785510377600566 19.396794862131834 60-61 20.988324841556892 30.962040784847026 28.539623227983334 19.51001114561274 62-63 20.39057148324696 30.72844365485598 29.28731517476029 19.593669687136767 64-65 20.787470937357103 29.667134211678388 29.90468194294974 19.640712908014773 66-67 21.013560432492564 29.65721483524158 29.341381953556184 19.98784277870967 68-69 21.22182884559941 30.347522297890368 29.456306414260318 18.9743424422499 70-71 20.497941660099862 30.96629520475942 29.581451450334118 18.9543116848066 72-73 20.292759774449838 30.379798602717756 29.649373426883063 19.67806819594935 74-75 20.570233295204382 29.3899828702937 29.33777075248424 20.70201308201767 76-77 20.559177304730607 28.74898100561991 28.93207723092405 21.759764458725428 78-79 19.88495897280871 29.2915574676317 29.34159956495271 21.48188399460688 80-81 19.425460591041524 29.906810020321185 28.979773583925194 21.687955804712097 82-83 18.603784551687845 30.11052709591783 29.036466530433465 22.24922182196086 84-85 18.36138824973679 30.52702283068595 30.00505224161057 21.106536677966687 86-87 18.798990624682986 30.192408630095663 30.62099669232859 20.387604052892762 88-89 19.56584166950531 29.98490036154181 30.093013106619438 20.35624486233344 90-91 19.509256121577334 30.191928139980394 29.48732245258344 20.811493285858827 92-93 18.90106620613163 30.57012867005516 29.732029979644746 20.796775144168468 94-95 19.064753594631544 30.046073721619564 29.802169697961155 21.08700298578773 96-97 19.382780906871382 30.678537185135156 29.391319543849704 20.547362364143755 98-99 20.180878654121692 30.49453663576897 28.731041801713776 20.593542908395566 100-101 19.902154263614637 30.97174025571652 27.982914475347215 21.143191005321633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13476.0 1 7569.0 2 1853.0 3 2042.5 4 1651.0 5 1111.5 6 981.5 7 1016.0 8 1034.0 9 984.0 10 902.0 11 856.5 12 813.5 13 804.5 14 824.5 15 835.5 16 892.0 17 993.5 18 1108.0 19 1279.5 20 1577.0 21 2051.0 22 2732.0 23 3795.5 24 5406.5 25 7547.5 26 10228.5 27 13852.0 28 18592.5 29 24288.0 30 31925.5 31 41340.5 32 58263.5 33 92747.5 34 141075.5 35 185908.5 36 242788.5 37 328636.0 38 409593.5 39 451899.0 40 454639.0 41 443687.5 42 444756.5 43 439153.5 44 390504.5 45 323425.0 46 277800.5 47 242237.0 48 205184.0 49 164853.5 50 121243.0 51 91036.5 52 73902.0 53 56995.5 54 41137.5 55 29907.5 56 22307.0 57 17087.5 58 13096.0 59 9725.5 60 7276.5 61 5719.5 62 4594.0 63 3523.5 64 2604.5 65 1973.0 66 1553.5 67 1271.0 68 1069.5 69 895.0 70 722.0 71 580.0 72 444.5 73 319.0 74 231.5 75 178.5 76 139.0 77 108.5 78 79.5 79 58.0 80 51.0 81 37.0 82 22.5 83 14.0 84 7.0 85 5.0 86 6.0 87 4.0 88 2.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14866665478710125 2 0.0 3 0.0 4 0.0162372564598884 5 2.1649675279851197E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.4147714735218642E-4 24-25 0.0 26-27 0.0 28-29 6.661438547646522E-5 30-31 0.0010741569658080017 32-33 9.575817912241876E-4 34-35 1.4988236732204675E-4 36-37 0.0015654380586969329 38-39 0.0074691379715486625 40-41 0.015621073394231095 42-43 0.005470706407254706 44-45 0.00198177796792484 46-47 5.079346892580474E-4 48-49 0.0059286803074054045 50-51 0.0023731374825990735 52-53 0.010316902950667551 54-55 0.021924459619941616 56-57 0.018743622713440402 58-59 0.018810237098916865 60-61 0.024147714735218644 62-63 0.007594039944317035 64-65 0.01086647163084839 66-67 0.02818621185472935 68-69 0.040026918873171044 70-71 0.038286618052598385 72-73 0.028286133432944047 74-75 0.03782031735426313 76-77 0.030884094466526187 78-79 0.012723347626004858 80-81 0.010491765712543273 82-83 0.004979425314365776 84-85 0.007385869989703081 86-87 0.023181806145809898 88-89 0.020883609846871848 90-91 0.01541290343961714 92-93 0.030576002933697537 94-95 0.0322746697633474 96-97 0.03321559795820247 98-99 0.023356668907685618 100-101 0.023006943383934178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6004709.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.71773563432002 #Duplication Level Percentage of deduplicated Percentage of total 1 80.57618476843041 28.779988659809213 2 8.99195088956122 6.423442494202729 3 3.4324760681884996 3.6780081832406117 4 1.635998174227418 2.3373660114114054 5 0.8935975330645205 1.5958640224739546 6 0.6584572182476507 1.411116050872761 7 0.5210362433158872 1.302716435625927 8 0.42558060739143244 1.216062050072042 9 0.2830832436057224 0.9099983211853506 >10 2.1039629244881497 14.473153844264145 >50 0.2371051710019034 5.92303794106798 >100 0.19826092537114573 14.980994107135942 >500 0.026704391135429384 6.579160985481653 >1k 0.015369840669541359 9.06625591441425 >5k 9.280314264593524E-5 0.2274882393124727 >10k+ 1.3919815879897708E-4 1.0953467394295389 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 29582 0.4926466877911985 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19367 0.3225302008806755 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17144 0.28550925615212996 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7565 0.12598445653236484 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6161 0.10260280723012555 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.494902583955359E-4 0.0 0.0 2 6.827974511337685E-4 0.0 0.0 3 7.827190293484663E-4 0.0 0.0 4 8.992942039322805E-4 0.0 0.0 5 9.82562185777862E-4 0.0 0.0 6 0.0011490981494690251 0.0 0.0 7 0.0013822484986366534 0.0 0.0 8 0.0017819348114954446 0.0 0.0 9 0.0020483923534013056 0.0 0.0 10-11 0.002939359759149028 0.0 0.0 12-13 0.004762928561567264 0.0 0.0 14-15 0.008118628229944198 0.0 0.0 16-17 0.015671034183338442 0.0 8.326798184558153E-6 18-19 0.02577976717939204 0.0 1.6653596369116306E-5 20-21 0.03279093125079 0.0 1.6653596369116306E-5 22-23 0.041126056233532715 0.0 1.6653596369116306E-5 24-25 0.04995246230916436 0.0 1.6653596369116306E-5 26-27 0.06051084240718409 0.0 1.6653596369116306E-5 28-29 0.07115249048704941 0.0 1.6653596369116306E-5 30-31 0.08375926293847046 0.0 1.6653596369116306E-5 32-33 0.09719038841016275 0.0 1.6653596369116306E-5 34-35 0.11222025913329023 0.0 1.6653596369116306E-5 36-37 0.12823269204219556 0.0 1.6653596369116306E-5 38-39 0.14437835372205382 0.0 1.6653596369116306E-5 40-41 0.16171474754230386 0.0 1.6653596369116306E-5 42-43 0.18152420042336773 0.0 1.6653596369116306E-5 44-45 0.2008090650188044 0.0 1.6653596369116306E-5 46-47 0.22183423043481376 0.0 1.6653596369116306E-5 48-49 0.2444498143040737 0.0 1.6653596369116306E-5 50-51 0.26906382973762755 0.0 1.6653596369116306E-5 52-53 0.2938527079330572 0.0 1.6653596369116306E-5 54-55 0.3196824359015566 0.0 1.6653596369116306E-5 56-57 0.3484765040237587 0.0 1.6653596369116306E-5 58-59 0.378378036304507 0.0 1.6653596369116306E-5 60-61 0.4105527844896397 0.0 1.6653596369116306E-5 62-63 0.44530884011198546 0.0 1.6653596369116306E-5 64-65 0.48281273913523537 0.0 1.6653596369116306E-5 66-67 0.5256291354002334 0.0 1.6653596369116306E-5 68-69 0.5741244080271001 0.0 1.6653596369116306E-5 70-71 0.628889759686939 0.0 1.6653596369116306E-5 72-73 0.6907911773909443 0.0 2.498039455367446E-5 74-75 0.76484139364622 0.0 3.330719273823261E-5 76-77 0.8525558857223556 0.0 3.330719273823261E-5 78-79 0.9624946021530769 0.0 3.330719273823261E-5 80-81 1.098895883214324 0.0 3.330719273823261E-5 82-83 1.2661396247511745 0.0 3.330719273823261E-5 84-85 1.4727441413064315 0.0 3.330719273823261E-5 86-87 1.716952478463153 0.0 3.330719273823261E-5 88-89 2.0024201006243603 0.0 3.330719273823261E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 140 0.0 50.960712 1 CGCGGAG 430 0.0 50.8817 1 CGAGTGG 355 0.0 49.573044 1 CGCGGGG 445 0.0 49.166584 1 CGACGGG 310 0.0 46.02903 1 CGGGTGG 765 0.0 42.278515 1 GTTACGG 285 0.0 41.722218 1 ATGACCG 160 0.0 41.617912 1 GTCCGGC 105 1.0149961E-9 40.768566 1 CACGGGG 535 0.0 39.117504 1 CGGGGTG 1135 0.0 38.55353 1 TCGGCCG 90 5.7063517E-7 36.941936 2 CGAGGGG 1165 0.0 36.7442 1 ACGCGAG 65 1.3184779E-4 36.587177 1 TGCGGCC 300 0.0 36.414196 2 CGAGGTG 380 0.0 35.04666 1 CGGATGG 150 2.5465852E-11 34.879776 1 CGGACCG 315 0.0 34.728783 1 CGCGCAA 180 0.0 34.351288 1 TCAGCCG 360 0.0 34.303226 2 >>END_MODULE