FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004709
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT295820.4926466877911985TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC193670.3225302008806755No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171440.28550925615212996No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG75650.12598445653236484No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA61610.10260280723012555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG1400.050.9607121
CGCGGAG4300.050.88171
CGAGTGG3550.049.5730441
CGCGGGG4450.049.1665841
CGACGGG3100.046.029031
CGGGTGG7650.042.2785151
GTTACGG2850.041.7222181
ATGACCG1600.041.6179121
GTCCGGC1051.0149961E-940.7685661
CACGGGG5350.039.1175041
CGGGGTG11350.038.553531
TCGGCCG905.7063517E-736.9419362
CGAGGGG11650.036.74421
ACGCGAG651.3184779E-436.5871771
TGCGGCC3000.036.4141962
CGAGGTG3800.035.046661
CGGATGG1502.5465852E-1134.8797761
CGGACCG3150.034.7287831
CGCGCAA1800.034.3512881
TCAGCCG3600.034.3032262
Produced by FastQC (version 0.11.2)