##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3892301 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67636495738639 33.0 33.0 33.0 33.0 33.0 2 32.71317428944987 33.0 33.0 33.0 33.0 33.0 3 32.733018078509346 33.0 33.0 33.0 33.0 33.0 4 36.669607258020385 37.0 37.0 37.0 37.0 37.0 5 36.670133168015525 37.0 37.0 37.0 37.0 37.0 6 36.537299402076044 37.0 37.0 37.0 37.0 37.0 7 36.497718701611205 37.0 37.0 37.0 37.0 37.0 8 36.42639174102928 37.0 37.0 37.0 37.0 37.0 9 36.43808842121922 37.0 37.0 37.0 37.0 37.0 10-11 36.40358299627907 37.0 37.0 37.0 37.0 37.0 12-13 36.38828433361141 37.0 37.0 37.0 37.0 37.0 14-15 38.27206554683207 40.0 37.0 40.0 37.0 40.0 16-17 38.23409880171138 40.0 37.0 40.0 37.0 40.0 18-19 38.20465452183683 40.0 37.0 40.0 37.0 40.0 20-21 38.13890639495763 40.0 37.0 40.0 37.0 40.0 22-23 38.05832102912905 40.0 37.0 40.0 37.0 40.0 24-25 38.0343309779999 40.0 37.0 40.0 35.0 40.0 26-27 37.95489981376055 40.0 37.0 40.0 33.0 40.0 28-29 37.83695197262493 40.0 37.0 40.0 33.0 40.0 30-31 37.802721834719364 40.0 37.0 40.0 33.0 40.0 32-33 37.698053285190426 40.0 37.0 40.0 33.0 40.0 34-35 37.631702044626046 40.0 37.0 40.0 33.0 40.0 36-37 37.540878647360515 40.0 37.0 40.0 33.0 40.0 38-39 37.44476891689517 40.0 37.0 40.0 33.0 40.0 40-41 37.366755936912384 40.0 37.0 40.0 33.0 40.0 42-43 37.25603081570516 40.0 37.0 40.0 33.0 40.0 44-45 37.10756465134634 40.0 37.0 40.0 33.0 40.0 46-47 37.023014021783 38.5 37.0 40.0 33.0 40.0 48-49 36.84175928326201 37.0 37.0 40.0 33.0 40.0 50-51 36.70290465716809 37.0 37.0 40.0 33.0 40.0 52-53 36.56370820242319 37.0 37.0 40.0 33.0 40.0 54-55 36.389476558981436 37.0 37.0 40.0 33.0 40.0 56-57 36.18761742732641 37.0 37.0 40.0 33.0 40.0 58-59 35.96641845016611 37.0 37.0 40.0 33.0 40.0 60-61 35.67985299698044 37.0 37.0 40.0 27.0 40.0 62-63 35.406362072203564 37.0 33.0 40.0 27.0 40.0 64-65 35.15738518167018 37.0 33.0 37.0 27.0 40.0 66-67 34.83861923833743 37.0 33.0 37.0 27.0 40.0 68-69 34.55123627386474 37.0 33.0 37.0 27.0 40.0 70-71 34.229410700765435 37.0 33.0 37.0 27.0 40.0 72-73 33.96149706818666 37.0 33.0 37.0 27.0 37.0 74-75 33.57829610299923 37.0 33.0 37.0 27.0 37.0 76-77 30.87141359314195 33.0 30.0 35.0 24.5 37.0 78-79 33.33161489823115 35.0 33.0 37.0 27.0 37.0 80-81 33.74277952809919 37.0 33.0 37.0 27.0 37.0 82-83 33.68813306062404 37.0 33.0 37.0 27.0 37.0 84-85 33.5444749776546 37.0 33.0 37.0 27.0 37.0 86-87 33.369913837598894 37.0 33.0 37.0 27.0 37.0 88-89 33.26330016614851 37.0 33.0 37.0 27.0 37.0 90-91 33.134331337684316 37.0 33.0 37.0 27.0 37.0 92-93 32.94366558495861 33.0 33.0 37.0 27.0 37.0 94-95 32.82527070748125 33.0 33.0 37.0 27.0 37.0 96-97 32.65917486854177 33.0 33.0 37.0 27.0 37.0 98-99 32.568277735971606 33.0 33.0 37.0 27.0 37.0 100-101 30.921101168691735 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10759969572148265 2306 2 0.0973484189963898 2306 3 0.0800264703369038 2306 4 0.14099664887174868 2306 5 0.1531621267404546 2306 6 0.151113138182005 2306 7 0.1667890914424106 2306 8 0.2466611774960441 2306 9 0.22389084597584485 2306 10-11 0.22176059696575123 2306 12-13 0.21502002246274543 2306 14-15 0.44453267565384635 2306 16-17 0.4770339133657089 2306 18-19 0.5458400639869438 2306 20-21 0.47925275565695813 2306 22-23 0.5557577353923406 2306 24-25 0.4828878640354972 2306 26-27 0.5963992172608528 2306 28-29 0.609797152740434 2306 30-31 0.568584214329114 2306 32-33 0.6312805320793728 2306 34-35 0.591531051379782 2306 36-37 0.6310587887558015 2306 38-39 0.7252789112184743 2306 40-41 0.600290922742154 2306 42-43 0.7105634645098675 2306 44-45 0.639679527646372 2306 46-47 0.7161163090266243 2306 48-49 0.6914849187630168 2306 50-51 0.7470677283160256 2306 52-53 0.7068494838654047 2306 54-55 0.6817933787607515 2306 56-57 0.7798210016684237 2306 58-59 0.7754467530358653 2306 60-61 0.6650160643838277 2306 62-63 0.6700140671686867 2306 64-65 0.616766340966123 2306 66-67 0.6342037505430795 2306 68-69 0.7552380546839714 2306 70-71 0.6290090548572209 2306 72-73 0.5554303948002328 2306 74-75 0.6626679560627906 2306 76-77 0.07265066994001401 2306 78-79 0.6307952649477855 2306 80-81 0.6531295498325846 2306 82-83 0.6340020708786369 2306 84-85 0.7043109296018883 2306 86-87 0.8173881965817458 2306 88-89 0.8162414583948561 2306 90-91 0.7176395269856286 2306 92-93 0.8051019775843429 2306 94-95 0.6939807407921776 2306 96-97 0.6773629773406569 2306 98-99 0.6516765494959387 2306 100-101 0.5292386531589912 2307 1 0.16077848432219355 2307 2 0.12330492787448577 2307 3 0.11507205658270436 2307 4 0.08499296449135585 2307 5 0.09204428229896422 2307 6 0.12625177708027024 2307 7 0.1563750824678607 2307 8 0.14244649374583673 2307 9 0.15946293850915083 2307 10-11 0.21187058331467767 2307 12-13 0.17824229973563632 2307 14-15 0.32248861619666513 2307 16-17 0.36175053270171986 2307 18-19 0.49986125039907847 2307 20-21 0.5010496382644973 2307 22-23 0.45103510199125907 2307 24-25 0.4196587275437764 2307 26-27 0.3670511308910349 2307 28-29 0.49648053058724173 2307 30-31 0.4647297620530466 2307 32-33 0.35969209010806225 2307 34-35 0.44834069397161613 2307 36-37 0.5247928923630809 2307 38-39 0.464446153059221 2307 40-41 0.3882432560707869 2307 42-43 0.39970826078188537 2307 44-45 0.5315203031300584 2307 46-47 0.5725303216757638 2307 48-49 0.5869074313316176 2307 50-51 0.5677423937300716 2307 52-53 0.528878538781413 2307 54-55 0.5890767667738928 2307 56-57 0.48017351652138984 2307 58-59 0.45975206421474013 2307 60-61 0.38365880870828306 2307 62-63 0.4102882154466272 2307 64-65 0.4596400719340892 2307 66-67 0.38790861673012955 2307 68-69 0.4454672199599088 2307 70-71 0.32917061395766467 2307 72-73 0.3846203516790254 2307 74-75 0.3945120019240278 2307 76-77 0.09995997600142204 2307 78-79 0.3558887371149737 2307 80-81 0.39245847325982197 2307 82-83 0.4811840036962636 2307 84-85 0.461368116501518 2307 86-87 0.40835436042276285 2307 88-89 0.38792580694361334 2307 90-91 0.33889673605523285 2307 92-93 0.4444614000381222 2307 94-95 0.28321035865407396 2307 96-97 0.23031602415053953 2307 98-99 0.3417210670509476 2307 100-101 0.37821457791336144 2201 1 0.13850987081769972 2201 2 0.12299563999244612 2201 3 0.09410386580965024 2201 4 0.12612779679359676 2201 5 0.12190965827868183 2201 6 0.1458037189190975 2201 7 0.2008681906377916 2201 8 0.18029498554845702 2201 9 0.13488372906174817 2201 10-11 0.1692526398066363 2201 12-13 0.23418612767535762 2201 14-15 0.3980419570701059 2201 16-17 0.33810540001858413 2201 18-19 0.3383537518494677 2201 20-21 0.4311132982151733 2201 22-23 0.3516308545212752 2201 24-25 0.3471902283378583 2201 26-27 0.36252843687844205 2201 28-29 0.41051349066607656 2201 30-31 0.2539368461933691 2201 32-33 0.3868412504307912 2201 34-35 0.4400135766064892 2201 36-37 0.39395790514328866 2201 38-39 0.4380591542312757 2201 40-41 0.45524449630037367 2201 42-43 0.3417849561964843 2201 44-45 0.3496122306023395 2201 46-47 0.5936666656467509 2201 48-49 0.4016453010164227 2201 50-51 0.47058142392275215 2201 52-53 0.39419005027573206 2201 54-55 0.5138775736356394 2201 56-57 0.4957631990059639 2201 58-59 0.3820835271145029 2201 60-61 0.5056049328796774 2201 62-63 0.5657423698737389 2201 64-65 0.515333665114845 2201 66-67 0.5064028692538258 2201 68-69 0.41059897283558655 2201 70-71 0.35528742764721954 2201 72-73 0.41582736217394967 2201 74-75 0.3877180997175884 2201 76-77 -0.005455719794280611 2201 78-79 0.5093732760839416 2201 80-81 0.4900376030197009 2201 82-83 0.4968578197808995 2201 84-85 0.5305157325508816 2201 86-87 0.5265867499663486 2201 88-89 0.5811858163624635 2201 90-91 0.3941297034758051 2201 92-93 0.3695569025043852 2201 94-95 0.44114907633260714 2201 96-97 0.48231616368919106 2201 98-99 0.5354408472137209 2201 100-101 0.3330898407311089 1101 1 0.05971038537921203 1101 2 0.03240435023103316 1101 3 0.05267917708205516 1101 4 0.0983671756719815 1101 5 0.038014300524402245 1101 6 0.051078250749647225 1101 7 0.011084253965890412 1101 8 0.16160413283270714 1101 9 0.09838219463490105 1101 10-11 0.1087156664065958 1101 12-13 0.09631293586026146 1101 14-15 0.2421128239625432 1101 16-17 0.20492530210820092 1101 18-19 0.1795554270760391 1101 20-21 0.23905501072120927 1101 22-23 0.23783524487505048 1101 24-25 0.2806855020821004 1101 26-27 0.3274245366293158 1101 28-29 0.269056054602828 1101 30-31 0.3143542987270038 1101 32-33 0.3090787639546093 1101 34-35 0.27810978192261615 1101 36-37 0.17513868839876778 1101 38-39 0.2665024951227437 1101 40-41 0.15116199512244322 1101 42-43 0.16664612545599056 1101 44-45 0.29646067952547384 1101 46-47 0.3872258036042311 1101 48-49 0.3940643619002131 1101 50-51 0.3502536189476402 1101 52-53 0.34326379810467245 1101 54-55 0.37995547913263295 1101 56-57 0.23753594907746844 1101 58-59 0.296931473082779 1101 60-61 0.262755804138429 1101 62-63 0.24776850418202656 1101 64-65 0.1855769764369981 1101 66-67 0.252651779403152 1101 68-69 0.26023066894030933 1101 70-71 0.0638630843556598 1101 72-73 -0.0048936871778053614 1101 74-75 0.19355817941659126 1101 76-77 -0.05239702192230311 1101 78-79 0.2362176626793655 1101 80-81 0.4009572504538781 1101 82-83 0.24210634718710367 1101 84-85 0.3435229190020479 1101 86-87 0.4100670317868804 1101 88-89 0.41339924608627143 1101 90-91 0.412500682427698 1101 92-93 0.4450339842055371 1101 94-95 0.39874473102369734 1101 96-97 0.3504625401343233 1101 98-99 0.2991100963601525 1101 100-101 0.25210996307072975 2304 1 0.16081679886233502 2304 2 0.12927897414463274 2304 3 0.12950152825649752 2304 4 0.11989632334901046 2304 5 0.16112863602225502 2304 6 0.11228941901135414 2304 7 0.06667178594958756 2304 8 0.13425905426662155 2304 9 0.08583987673920745 2304 10-11 0.16847677632585345 2304 12-13 0.18486591237153505 2304 14-15 0.3033907066811423 2304 16-17 0.3052304880453178 2304 18-19 0.27578449050971443 2304 20-21 0.3431138659534483 2304 22-23 0.3788918448970193 2304 24-25 0.31564322343019313 2304 26-27 0.2856606325516253 2304 28-29 0.339787768301008 2304 30-31 0.2860693311947031 2304 32-33 0.3161701182842478 2304 34-35 0.3144246846241572 2304 36-37 0.4371865344374939 2304 38-39 0.4254856621495833 2304 40-41 0.38666075763466523 2304 42-43 0.47349272929769626 2304 44-45 0.49214660879244576 2304 46-47 0.5586688765506054 2304 48-49 0.565130040320831 2304 50-51 0.48433201759137035 2304 52-53 0.43865173613087194 2304 54-55 0.43058586534393584 2304 56-57 0.5938925286926775 2304 58-59 0.5742758975855651 2304 60-61 0.4484012076431654 2304 62-63 0.47713084478679946 2304 64-65 0.575763672266163 2304 66-67 0.6262475054919037 2304 68-69 0.6300393685054928 2304 70-71 0.42139959416922323 2304 72-73 0.4268914347134327 2304 74-75 0.4076128224811697 2304 76-77 0.2833408661334538 2304 78-79 0.5078488442316385 2304 80-81 0.5482137165236907 2304 82-83 0.4775554008883134 2304 84-85 0.6978224695297399 2304 86-87 0.6055044169987696 2304 88-89 0.6013377955308385 2304 90-91 0.47867357490492424 2304 92-93 0.6788446093330691 2304 94-95 0.5284729546400939 2304 96-97 0.27255051949731524 2304 98-99 0.400147179133171 2304 100-101 0.5905857601122726 2202 1 0.10538584374904048 2202 2 0.023109943220937623 2202 3 0.04978803170143209 2202 4 0.07997412347729238 2202 5 0.07790838849886939 2202 6 0.11746700373117136 2202 7 0.1190414245549718 2202 8 0.09040794064410562 2202 9 0.11622505209857081 2202 10-11 0.05786503663702547 2202 12-13 0.11296824521179616 2202 14-15 0.11896938604251517 2202 16-17 0.10658144531124236 2202 18-19 0.1469786621179736 2202 20-21 0.11950794854754321 2202 22-23 0.08874365521538863 2202 24-25 0.05979985054955961 2202 26-27 0.12233581519793546 2202 28-29 0.16068748045944403 2202 30-31 0.2114081522805904 2202 32-33 0.09185768728114141 2202 34-35 0.01987287532391946 2202 36-37 0.25013042351304904 2202 38-39 0.16804327046106238 2202 40-41 0.12779224843290393 2202 42-43 0.184589210765445 2202 44-45 0.09869489534244025 2202 46-47 0.23302356019395631 2202 48-49 0.14843628396656783 2202 50-51 0.133646012160348 2202 52-53 0.1458000319895305 2202 54-55 -0.018143173783030875 2202 56-57 0.050489758490314784 2202 58-59 -0.08597796733107543 2202 60-61 0.08333530516022591 2202 62-63 0.07533859832809497 2202 64-65 0.21831724439600464 2202 66-67 0.18692476949890846 2202 68-69 0.17751559139580309 2202 70-71 -0.041366812000099173 2202 72-73 0.05449549911198659 2202 74-75 -0.008899123889250404 2202 76-77 0.07292308133538583 2202 78-79 0.24415637072862495 2202 80-81 0.09583175120139487 2202 82-83 0.02675554604540764 2202 84-85 0.16733911677910385 2202 86-87 0.2342077045596227 2202 88-89 0.08318425045433031 2202 90-91 0.1276075866076738 2202 92-93 0.1069269430751163 2202 94-95 0.20474246103326266 2202 96-97 0.042808151516823045 2202 98-99 -0.17871999194711918 2202 100-101 0.05708254353003639 1102 1 0.06502376482681171 1102 2 0.06155548677408973 1102 3 0.06960955833255156 1102 4 0.08859935964756716 1102 5 0.037852224316850425 1102 6 0.05224399629627641 1102 7 0.04797965151598049 1102 8 0.07081364470955975 1102 9 0.022467146281876182 1102 10-11 0.024595194001960863 1102 12-13 0.05991361879481616 1102 14-15 -0.028457929276825666 1102 16-17 -0.04861877446848695 1102 18-19 0.08178877444319 1102 20-21 0.06550312763368282 1102 22-23 0.03783629980893721 1102 24-25 0.004162000988408465 1102 26-27 -0.004706622517225867 1102 28-29 -0.06327148789901571 1102 30-31 -0.005844163109628653 1102 32-33 0.043932724921880606 1102 34-35 0.03291812266591876 1102 36-37 0.0036633685772500257 1102 38-39 -0.027251825420052 1102 40-41 0.023230684745456642 1102 42-43 -0.07856079110911907 1102 44-45 0.09301637266804619 1102 46-47 0.14871104765098409 1102 48-49 0.05806281594254159 1102 50-51 -0.055358219607718695 1102 52-53 -0.1247795078907572 1102 54-55 -0.015270142892575223 1102 56-57 0.07641772612321063 1102 58-59 0.06432639578184762 1102 60-61 0.08238538633317205 1102 62-63 -0.08392790885964274 1102 64-65 -0.19349542803546882 1102 66-67 -0.13177380429569752 1102 68-69 0.016336447597915082 1102 70-71 0.0018187166259266974 1102 72-73 -0.11931085843271916 1102 74-75 -0.03862483714159737 1102 76-77 0.12014016297177221 1102 78-79 0.09864665407624784 1102 80-81 -0.028094042262992502 1102 82-83 -0.12722077848508206 1102 84-85 0.1410145390343871 1102 86-87 0.06630261090592171 1102 88-89 0.13738869570110523 1102 90-91 -0.15067093883917693 1102 92-93 -0.2176070489043127 1102 94-95 -0.09981314818602272 1102 96-97 -0.3116432230044168 1102 98-99 -0.26270205513567646 1102 100-101 -0.3128037812619411 2305 1 0.02355883777793366 2305 2 0.017692524450424685 2305 3 0.020946079341285895 2305 4 3.627768306273538E-4 2305 5 0.0375741985166016 2305 6 0.0011451920080105538 2305 7 0.045899155122683055 2305 8 0.05882942039688288 2305 9 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-0.14609264526103516 1213 94-95 -0.257551257758152 1213 96-97 -0.18166280444594918 1213 98-99 -0.26794573262121446 1213 100-101 -0.047129855609497895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 13.0 8 45.0 9 190.0 10 310.0 11 191.0 12 183.0 13 225.0 14 455.0 15 916.0 16 1684.0 17 2777.0 18 4061.0 19 5116.0 20 6796.0 21 8565.0 22 10896.0 23 14081.0 24 17227.0 25 21946.0 26 27967.0 27 35381.0 28 45857.0 29 59869.0 30 72621.0 31 89900.0 32 120936.0 33 170868.0 34 262196.0 35 447427.0 36 851964.0 37 1284409.0 38 326439.0 39 789.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.93343412289156 32.25220408547627 15.827862892581418 18.986498899050748 2 30.777321692232952 24.69228356183142 29.93029059160635 14.600104154329276 3 32.56166468112307 22.983833984062382 24.286533852340813 20.167967482473735 4 28.467717462497916 22.794334737083915 28.610584993718714 20.12736280669946 5 31.447702988749317 21.06347485816216 27.881942205335307 19.606879947753217 6 28.500904734757153 21.474623879294022 30.178113152091786 19.846358233857043 7 31.210844176747894 22.339176749177415 24.55809558407739 21.891883489997305 8 27.593420961020232 25.279596824603235 25.321474366961855 21.805507847414678 9 26.765298983814457 22.662584419858586 28.33701710119541 22.235099495131543 10-11 22.017837263870394 29.253570060486073 31.05390615987818 17.67468651576535 12-13 22.066651577049154 28.638625327280703 30.13155971236551 19.16316338330463 14-15 22.080550810433213 28.443599300259663 30.417932734390273 19.057917154916847 16-17 22.396790484600242 27.803951955411467 30.6275259801336 19.171731579854693 18-19 21.590095421705566 27.871585470907828 31.023654131579235 19.51466497580737 20-21 21.992723584327113 28.09976669327475 30.54708513036376 19.360424592034377 22-23 21.586324564980078 28.936041043158877 29.97853707313401 19.49909731872703 24-25 21.328193785629633 28.160746047132534 30.527058416088583 19.984001751149254 26-27 21.257413031520432 28.296051615740918 30.48669668661288 19.95983866612577 28-29 21.33210441127354 29.141368856460193 30.087570331166663 19.43895640109961 30-31 20.6919196142805 29.453208208036543 30.278770025166622 19.576102152516334 32-33 20.535965700168333 29.81911042005373 29.9660942834299 19.678829596348038 34-35 21.033718384358032 29.397440792046243 30.28947166943538 19.279369154160346 36-37 20.587603945860863 28.975750536870827 31.14089723103501 19.295748286233305 38-39 20.94997871924134 29.038581186832985 30.459118397199802 19.552321696725873 40-41 20.770453990365855 29.10937295838468 30.3671461126231 19.75302693862637 42-43 21.0927291971479 29.11640702189998 30.02110310924967 19.76976067170245 44-45 20.633311199466732 28.79355268598903 30.416583006303206 20.15655310824103 46-47 21.050363257338496 29.014071950593507 30.006240555026586 19.929324237041406 48-49 20.664034990129927 29.764605920398633 29.940759015320644 19.63060007415079 50-51 21.172168776353963 29.324696238943282 29.395594372751273 20.107540611951478 52-53 20.697738236006 29.10680543629131 29.298369081774077 20.897087245928613 54-55 21.26897980134769 29.149852658601596 29.565145327379287 20.016022212671423 56-57 20.96439226327678 28.83272047014444 29.712082784069153 20.49080448250963 58-59 21.0066544891045 29.329333419159582 29.61245724266227 20.05155484907365 60-61 21.05499712827181 29.476201917826174 29.342457762467607 20.12634319143441 62-63 20.586533094297373 29.464231000720066 29.232821752700556 20.71641415228201 64-65 20.889620151817994 29.23968697013307 29.302509804344773 20.568183073704162 66-67 21.02248561706375 29.489947042628796 29.058940806318184 20.428626533989267 68-69 21.056097933964484 29.696205264490327 29.145211056380653 20.102485745164536 70-71 20.973456740624684 30.22489138636845 28.932810671996084 19.86884120101078 72-73 20.67181576624669 30.14757157151189 28.939894462689885 20.24071819955153 74-75 20.852373721745263 29.586424772774706 28.88092376682536 20.68027773865467 76-77 20.907735026211576 29.19973390725483 28.984898838369215 20.907632228164378 78-79 20.616529185966765 28.84151344140105 29.673630762376153 20.868326610256027 80-81 20.56248311398266 29.25641655811867 29.35983638854355 20.821263939355124 82-83 20.10785385902305 29.573752700489464 29.211302276639316 21.10709116384817 84-85 20.14731702566884 29.52514693868689 29.34188708700854 20.985648948635728 86-87 20.281420640751566 29.307583211076533 29.82959341871651 20.581402729455398 88-89 20.410452143125678 29.83095930632208 29.5034754049157 20.255113145636543 90-91 20.71511476869859 29.603961973401322 28.932275559993492 20.748647697906595 92-93 20.375188055331826 29.6659438283157 29.001948528221277 20.956919588131196 94-95 19.95762344878667 29.827088559513363 28.97151346918467 21.24377452251529 96-97 20.0366947389901 30.25416722243339 28.834437028224723 20.87470101035178 98-99 20.301011754224273 29.99086834731734 28.74428918364927 20.96383071480912 100-101 20.423782996462457 29.99020661447982 28.322812703493494 21.263197685564226 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 222.0 1 142.0 2 97.0 3 166.5 4 167.0 5 97.0 6 69.5 7 84.0 8 82.5 9 79.5 10 90.5 11 105.0 12 103.5 13 110.5 14 130.5 15 136.0 16 146.5 17 186.5 18 232.5 19 303.0 20 502.0 21 779.5 22 1178.0 23 1772.5 24 2682.5 25 4418.0 26 7350.5 27 11571.0 28 16469.5 29 21790.5 30 27357.0 31 32053.0 32 39669.0 33 55281.0 34 77650.5 35 110259.0 36 154121.5 37 206005.5 38 257366.5 39 276348.0 40 282407.5 41 272086.0 42 239481.5 43 224571.5 44 211709.5 45 202171.0 46 206088.5 47 192319.5 48 157771.0 49 128374.5 50 103452.5 51 83994.0 52 69514.5 53 55428.0 54 45090.0 55 34926.0 56 23787.5 57 15334.5 58 10145.0 59 7262.0 60 5420.5 61 3944.5 62 2788.0 63 1937.5 64 1364.5 65 985.0 66 725.0 67 540.0 68 400.5 69 279.5 70 198.0 71 142.0 72 109.5 73 79.5 74 59.5 75 42.0 76 23.5 77 17.0 78 14.0 79 11.0 80 11.5 81 9.0 82 6.5 83 7.0 84 4.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14528681106625618 2 0.0 3 0.0 4 0.01500397836652407 5 1.0276697511317856E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.055339502263571E-4 24-25 0.0 26-27 0.0 28-29 2.569174377829464E-5 30-31 0.001091899110577522 32-33 9.505945197969016E-4 34-35 7.707523133488392E-5 36-37 0.001811267936369772 38-39 0.0073863763362597085 40-41 0.015967418758210117 42-43 0.005665029503113967 44-45 0.0019397266552612451 46-47 4.8814313178759816E-4 48-49 0.005960484556564356 50-51 0.00249209914649458 52-53 0.009839937867086847 54-55 0.02128560972031711 56-57 0.018780664701933382 58-59 0.0184338261609264 60-61 0.023533637300917887 62-63 0.007155150642255056 64-65 0.010379464486431034 66-67 0.027181864917435728 68-69 0.038948683567894674 70-71 0.03712456975963575 72-73 0.027503011714664412 74-75 0.03722733673474893 76-77 0.030226336555163644 78-79 0.012383420501138016 80-81 0.01021246815187212 82-83 0.004688743239538772 84-85 0.007360684592481414 86-87 0.02317395288802176 88-89 0.020861695947975246 90-91 0.015517813242089961 92-93 0.029776731039043488 94-95 0.03170361182241559 96-97 0.03288543203621714 98-99 0.02290418957834967 100-101 0.02309687765668688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3892301.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.27965922233628 #Duplication Level Percentage of deduplicated Percentage of total 1 87.30255860017702 36.91122426853527 2 5.811563353844447 4.9142183629912175 3 1.8619010335271666 2.3616162360973294 4 1.0550308160275157 1.784253734828269 5 0.5339713856759188 1.128806411042827 6 0.44982737647850823 1.1411128911833335 7 0.3451926417691637 1.021623908204077 8 0.2568776677142948 0.8688560202231135 9 0.22571153782347816 0.8588706211553511 >10 1.7352644346979018 14.856437283548123 >50 0.2213297010107341 6.546562064333669 >100 0.16593866780358302 13.859360205623581 >500 0.025162943328027224 7.469838265107927 >1k 0.00960882167238366 5.768165843303614 >5k 0.0 0.0 >10k+ 6.101844983420763E-5 0.5090538838221924 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 19732 0.5069494882333098 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.47827544683723E-4 0.0 0.0 2 9.505945197969016E-4 0.0 0.0 3 0.001207511957579848 0.0 0.0 4 0.0015158128829193837 0.0 0.0 5 0.0017984220644806247 0.0 0.0 6 0.0028260918156124103 0.0 0.0 7 0.003494077153848071 0.0 0.0 8 0.004752972598984508 0.0 0.0 9 0.005241115730772106 0.0 0.0 10-11 0.007668985517820949 0.0 0.0 12-13 0.011227292031114756 0.0 0.0 14-15 0.016172952708436474 0.0 0.0 16-17 0.02524213826217448 0.0 0.0 18-19 0.036507967908956676 0.0 0.0 20-21 0.04727280855206213 0.0 0.0 22-23 0.06013152631309861 0.0 0.0 24-25 0.07373530464370562 0.0 0.0 26-27 0.08900904631990178 0.0 0.0 28-29 0.1049250815905553 0.0 0.0 30-31 0.12491325825006852 0.0 0.0 32-33 0.14273048256031584 0.0 0.0 34-35 0.1628471179387206 0.0 0.0 36-37 0.1876396506847749 0.0 0.0 38-39 0.21434621834231216 0.0 0.0 40-41 0.24443125030669519 0.0 0.0 42-43 0.27721391536779916 0.0 0.0 44-45 0.31559738057257136 0.0 0.0 46-47 0.3551369742473668 0.0 0.0 48-49 0.39883863041424594 0.0 0.0 50-51 0.4458159839128577 0.0 0.0 52-53 0.4953111283017423 0.0 0.0 54-55 0.5488013388481519 0.0 0.0 56-57 0.6062095403207511 0.0 0.0 58-59 0.6680881052107738 0.0 0.0 60-61 0.7305190425920298 0.0 0.0 62-63 0.7980240993694989 0.0 0.0 64-65 0.8682781727312456 0.0 0.0 66-67 0.9451607159877924 0.0 0.0 68-69 1.0293140227335964 0.0 0.0 70-71 1.1174623956369252 0.0 0.0 72-73 1.2099912108544535 0.0 1.284587188914732E-5 74-75 1.312411347426625 0.0 2.569174377829464E-5 76-77 1.420188212576571 0.0 5.138348755658928E-5 78-79 1.535274378831442 0.0 5.138348755658928E-5 80-81 1.6589158957644847 0.0 5.138348755658928E-5 82-83 1.7925643468991734 0.0 5.138348755658928E-5 84-85 1.935705897359942 0.0 7.707523133488391E-5 86-87 2.0882377801716774 0.0 7.707523133488391E-5 88-89 2.255092810139812 0.0 7.707523133488391E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGGCG 165 0.0 51.87123 1 CGGACGG 55 8.138213E-7 51.871227 1 TCGCCGG 50 2.8322054E-5 47.488766 2 CCGGGCG 85 7.1649993E-9 44.75165 1 CGAGTCG 45 9.579552E-4 42.265446 1 GTTCGCC 45 9.579552E-4 42.265446 1 CGTGGCA 105 1.0186341E-9 40.755962 1 CGCGCAA 50 0.0016081857 38.038902 1 CGGTCGC 50 0.0016081857 38.038902 1 GCGCGCC 50 0.0016181691 37.991013 2 TCGGCAC 50 0.0016181691 37.991013 2 TTGTGTT 24835 0.0 36.319607 1 CGGACCC 105 4.620597E-8 36.227524 1 CGCGGGG 150 2.5465852E-11 34.86899 1 CGGGCTG 355 0.0 34.82435 1 GGCGCCG 55 0.0025674638 34.58082 1 CGCGTGG 55 0.0025674638 34.58082 1 CGGCGAG 70 2.0419198E-4 33.963306 1 CACGGGT 70 2.0419198E-4 33.963306 1 GGTACGG 85 1.6218024E-5 33.563736 1 >>END_MODULE