FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892301
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT197320.5069494882333098TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGCG1650.051.871231
CGGACGG558.138213E-751.8712271
TCGCCGG502.8322054E-547.4887662
CCGGGCG857.1649993E-944.751651
CGAGTCG459.579552E-442.2654461
GTTCGCC459.579552E-442.2654461
CGTGGCA1051.0186341E-940.7559621
CGCGCAA500.001608185738.0389021
CGGTCGC500.001608185738.0389021
GCGCGCC500.001618169137.9910132
TCGGCAC500.001618169137.9910132
TTGTGTT248350.036.3196071
CGGACCC1054.620597E-836.2275241
CGCGGGG1502.5465852E-1134.868991
CGGGCTG3550.034.824351
GGCGCCG550.002567463834.580821
CGCGTGG550.002567463834.580821
CGGCGAG702.0419198E-433.9633061
CACGGGT702.0419198E-433.9633061
GGTACGG851.6218024E-533.5637361
Produced by FastQC (version 0.11.2)