##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096019 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67999421192182 33.0 33.0 33.0 33.0 33.0 2 32.70615942602419 33.0 33.0 33.0 33.0 33.0 3 32.73915276359738 33.0 33.0 33.0 33.0 33.0 4 36.66249528830411 37.0 37.0 37.0 37.0 37.0 5 36.67069646536407 37.0 37.0 37.0 37.0 37.0 6 36.54955541293513 37.0 37.0 37.0 37.0 37.0 7 36.43416077226916 37.0 37.0 37.0 37.0 37.0 8 36.370334936575 37.0 37.0 37.0 37.0 37.0 9 36.415876000760974 37.0 37.0 37.0 37.0 37.0 10-11 36.355617811131005 37.0 37.0 37.0 37.0 37.0 12-13 36.33747661109315 37.0 37.0 37.0 37.0 37.0 14-15 38.169866044103735 40.0 37.0 40.0 37.0 40.0 16-17 38.1861437219862 40.0 37.0 40.0 37.0 40.0 18-19 38.13259027157133 40.0 37.0 40.0 37.0 40.0 20-21 38.11704062539668 40.0 37.0 40.0 37.0 40.0 22-23 37.89175001832999 40.0 37.0 40.0 33.0 40.0 24-25 37.978527263560075 40.0 37.0 40.0 35.0 40.0 26-27 37.912106482550655 40.0 37.0 40.0 33.0 40.0 28-29 37.683587536123 40.0 37.0 40.0 33.0 40.0 30-31 37.66462996512618 40.0 37.0 40.0 33.0 40.0 32-33 37.53811749863292 40.0 37.0 40.0 33.0 40.0 34-35 37.55013099079818 40.0 37.0 40.0 33.0 40.0 36-37 37.44350955210546 40.0 37.0 40.0 33.0 40.0 38-39 37.14417563328907 40.0 37.0 40.0 33.0 40.0 40-41 37.0007193108311 38.5 37.0 40.0 33.0 40.0 42-43 36.79086982347331 37.0 37.0 40.0 33.0 40.0 44-45 36.569200156717386 37.0 37.0 40.0 33.0 40.0 46-47 36.71490226642666 37.0 37.0 40.0 33.0 40.0 48-49 36.3334193039513 37.0 37.0 40.0 33.0 40.0 50-51 36.29198981014005 37.0 37.0 40.0 33.0 40.0 52-53 36.27752365214813 37.0 37.0 40.0 33.0 40.0 54-55 35.85063221511238 37.0 37.0 40.0 30.0 40.0 56-57 35.679040406405775 37.0 37.0 40.0 27.0 40.0 58-59 35.52420850776433 37.0 35.0 40.0 27.0 40.0 60-61 35.146625553654545 37.0 33.0 40.0 27.0 40.0 62-63 34.90896050702531 37.0 33.0 37.0 27.0 40.0 64-65 34.65437243763685 37.0 33.0 37.0 27.0 40.0 66-67 33.901324733472244 37.0 33.0 37.0 27.0 40.0 68-69 33.792666162578456 37.0 33.0 37.0 27.0 40.0 70-71 33.45702981796946 37.0 33.0 37.0 27.0 38.5 72-73 32.6951514186444 37.0 33.0 37.0 22.0 37.0 74-75 31.70609288896483 35.0 33.0 37.0 18.5 37.0 76-77 29.17092078569285 33.0 27.0 33.0 15.0 37.0 78-79 31.172156566222625 33.0 33.0 37.0 15.0 37.0 80-81 31.460584544216296 35.0 33.0 37.0 15.0 37.0 82-83 31.37567663505941 37.0 33.0 37.0 15.0 37.0 84-85 31.209595774444537 33.0 33.0 37.0 15.0 37.0 86-87 31.02372159214785 33.0 33.0 37.0 15.0 37.0 88-89 30.919504046971287 33.0 33.0 37.0 15.0 37.0 90-91 30.79316212206708 33.0 33.0 37.0 15.0 37.0 92-93 30.587079084462985 33.0 33.0 37.0 15.0 37.0 94-95 30.472197683541346 33.0 30.0 37.0 15.0 37.0 96-97 30.311979513045625 33.0 27.0 37.0 15.0 37.0 98-99 30.216681002280673 33.0 27.0 37.0 15.0 37.0 100-101 28.732748410135727 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1965999039333255 2306 2 0.15834805115313344 2306 3 0.15648703566927935 2306 4 0.1658878012573055 2306 5 0.13446404884379604 2306 6 0.17376300295475033 2306 7 0.2247958763772644 2306 8 0.3022748890798681 2306 9 0.19527886967335206 2306 10-11 0.21496885654364206 2306 12-13 0.21054134213508036 2306 14-15 0.44779693370017526 2306 16-17 0.4343410635574898 2306 18-19 0.5109890990757222 2306 20-21 0.48228280793029654 2306 22-23 0.48926321210380763 2306 24-25 0.36113796677725674 2306 26-27 0.5204030777947111 2306 28-29 0.47505397785700154 2306 30-31 0.5151375097876141 2306 32-33 0.5427118086755556 2306 34-35 0.3828102948135026 2306 36-37 0.5464980067045886 2306 38-39 0.6705272904560289 2306 40-41 0.7146403506071906 2306 42-43 0.6042305712032245 2306 44-45 0.7841347294618188 2306 46-47 0.537991284505047 2306 48-49 0.5888960140844759 2306 50-51 0.545057340365112 2306 52-53 0.6003591730791555 2306 54-55 0.5462195718015508 2306 56-57 0.595569095487896 2306 58-59 0.6821934958814353 2306 60-61 0.5559596550513319 2306 62-63 0.4735572384839273 2306 64-65 0.4397588035558968 2306 66-67 0.51754945059308 2306 68-69 0.6305197971085121 2306 70-71 0.5357875193130042 2306 72-73 0.45109571483694566 2306 74-75 0.27801321482367314 2306 76-77 -0.09741733217789772 2306 78-79 0.6156145863092135 2306 80-81 0.7128907589691345 2306 82-83 0.7610424994896583 2306 84-85 0.8187263625408896 2306 86-87 0.8435743815437 2306 88-89 0.9128356230378536 2306 90-91 0.7521650769456691 2306 92-93 0.7342499079673637 2306 94-95 0.6843433963209868 2306 96-97 0.5189334809887853 2306 98-99 0.8009418632919356 2306 100-101 0.6309820010884337 2307 1 0.1461413820378965 2307 2 0.16109084828948994 2307 3 0.16061914998166316 2307 4 0.19712201829748466 2307 5 0.13980573863796764 2307 6 0.1974091757345775 2307 7 0.1997698318891139 2307 8 0.25188109641899104 2307 9 0.2980294454301671 2307 10-11 0.24389699042480828 2307 12-13 0.2957836322720695 2307 14-15 0.44084993110821813 2307 16-17 0.4397924874617445 2307 18-19 0.5211071396412308 2307 20-21 0.4919439934476131 2307 22-23 0.4927524789966071 2307 24-25 0.46701165864943306 2307 26-27 0.39867669632560165 2307 28-29 0.48093780971656486 2307 30-31 0.5906420783380995 2307 32-33 0.5005554361796456 2307 34-35 0.5329779518652416 2307 36-37 0.5978685718491406 2307 38-39 0.5230074202478718 2307 40-41 0.4598815017318927 2307 42-43 0.3603291485491056 2307 44-45 0.502196547269854 2307 46-47 0.6293417363595566 2307 48-49 0.3212309059199825 2307 50-51 0.48979093650468997 2307 52-53 0.27296509427617366 2307 54-55 0.45936530147781696 2307 56-57 0.467672323337581 2307 58-59 0.5926649080622042 2307 60-61 0.2789607579485889 2307 62-63 0.37041134212292803 2307 64-65 0.3478252329746354 2307 66-67 0.23417861319680355 2307 68-69 0.5367561671597088 2307 70-71 0.20342818038462696 2307 72-73 0.14773660093293728 2307 74-75 0.16556575523777184 2307 76-77 0.26986106742650406 2307 78-79 0.5135668644726827 2307 80-81 0.3771988367886685 2307 82-83 0.3541652266739881 2307 84-85 0.47012720874367275 2307 86-87 0.42285297005745903 2307 88-89 0.44046917222790327 2307 90-91 0.32598099033628714 2307 92-93 0.40307403058064395 2307 94-95 0.3399353910093019 2307 96-97 0.22148389617265352 2307 98-99 0.3851457075972924 2307 100-101 0.15400074478566594 2201 1 0.12043352608159807 2201 2 0.11899305414351602 2201 3 0.11966083069757616 2201 4 0.13546101967807544 2201 5 0.14608616644515138 2201 6 0.12118539352456281 2201 7 0.07929295500138522 2201 8 0.19792272910194697 2201 9 0.07666743055707315 2201 10-11 0.10373421101924407 2201 12-13 0.13776662289898667 2201 14-15 0.23857198008947478 2201 16-17 0.25516640975202876 2201 18-19 0.22226463875071545 2201 20-21 0.39618554119308413 2201 22-23 0.3168151348956485 2201 24-25 0.3482399516770869 2201 26-27 0.32905423535476075 2201 28-29 0.26193487875514165 2201 30-31 0.20356446516698412 2201 32-33 0.399162860933167 2201 34-35 0.3826275490779949 2201 36-37 0.23370529404222395 2201 38-39 0.2503179124049453 2201 40-41 0.3462249478253625 2201 42-43 0.25322457509839325 2201 44-45 0.3967176040139009 2201 46-47 0.3212609763165091 2201 48-49 0.23208304859402773 2201 50-51 0.280561641918581 2201 52-53 0.14071300971724554 2201 54-55 0.46108152848442785 2201 56-57 0.3758195210426507 2201 58-59 0.3636556662362409 2201 60-61 0.25334754118156866 2201 62-63 0.29417888069077947 2201 64-65 0.3357675344570481 2201 66-67 0.3132082809181327 2201 68-69 0.28114189937455336 2201 70-71 0.2842486213081301 2201 72-73 0.07274610051990038 2201 74-75 0.20994796820638584 2201 76-77 0.07208406589087701 2201 78-79 0.5079599680816713 2201 80-81 0.5562048209339885 2201 82-83 0.43162145842681454 2201 84-85 0.5638001509238855 2201 86-87 0.5807624997482428 2201 88-89 0.4268227004862659 2201 90-91 0.3442367234975272 2201 92-93 0.43830763006093676 2201 94-95 0.5106638940027999 2201 96-97 0.5973556824231494 2201 98-99 0.5040845787657737 2201 100-101 0.3982250196901589 1101 1 0.09444794155977831 1101 2 0.13313626140643464 1101 3 0.12721282961653202 1101 4 0.10010682216141475 1101 5 0.1272009409026822 1101 6 0.07098941476893117 1101 7 0.019395766429845196 1101 8 0.10020562220363871 1101 9 0.12640573678262257 1101 10-11 0.11228078301981981 1101 12-13 0.06487322359860315 1101 14-15 0.15814608724598145 1101 16-17 0.24855670146397557 1101 18-19 0.2078779159192905 1101 20-21 0.27486793495715744 1101 22-23 0.2547568713325745 1101 24-25 0.1943507808204501 1101 26-27 0.20101873637506884 1101 28-29 0.24353266262270523 1101 30-31 0.3071010110022101 1101 32-33 0.3192913631535603 1101 34-35 0.4243037003174521 1101 36-37 0.41044089042814136 1101 38-39 0.37788930409139 1101 40-41 0.30537164192556787 1101 42-43 0.3033411247462183 1101 44-45 0.43617697934318045 1101 46-47 0.4839086641854493 1101 48-49 0.5227219964459024 1101 50-51 0.5062303947890854 1101 52-53 0.4505997417986407 1101 54-55 0.42137944193203225 1101 56-57 0.4665211274008456 1101 58-59 0.5124259829955093 1101 60-61 0.4410636587350396 1101 62-63 0.3219014490943195 1101 64-65 0.42571466483419584 1101 66-67 0.2489885401908296 1101 68-69 0.22969918525580368 1101 70-71 0.03536887335780392 1101 72-73 0.11860907062962411 1101 74-75 0.1123036545663787 1101 76-77 0.21185255115567259 1101 78-79 0.5974683280455366 1101 80-81 0.4994882151428577 1101 82-83 0.4967044628499089 1101 84-85 0.6338847761029527 1101 86-87 0.5258136440502206 1101 88-89 0.7459398992408133 1101 90-91 0.5981371954069665 1101 92-93 0.7020205125372279 1101 94-95 0.6007596271917812 1101 96-97 0.566153314752043 1101 98-99 0.544510174001477 1101 100-101 0.5575117503237337 2304 1 0.10853972139329926 2304 2 0.10375744771621953 2304 3 0.032321724135272234 2304 4 0.12471960365699175 2304 5 0.0705706428460573 2304 6 0.07938423971474151 2304 7 0.09271659012929945 2304 8 0.0639956878023824 2304 9 0.08234577660137177 2304 10-11 0.035578592988649405 2304 12-13 0.08421148087136743 2304 14-15 0.1408615845154486 2304 16-17 0.22019640864018442 2304 18-19 0.2108697009011209 2304 20-21 0.27652019787154813 2304 22-23 0.23008127272584744 2304 24-25 0.22016534778195052 2304 26-27 0.15566362951682322 2304 28-29 0.20760646177853914 2304 30-31 0.22709563985105063 2304 32-33 0.14111494965330706 2304 34-35 0.20841204696339588 2304 36-37 0.3442587415527356 2304 38-39 0.2347481258037476 2304 40-41 0.14274140687815162 2304 42-43 0.07035591731143853 2304 44-45 0.24019546870499653 2304 46-47 0.26412849718030174 2304 48-49 0.4043745590382528 2304 50-51 0.3413552644483815 2304 52-53 0.35144840673332567 2304 54-55 0.28657468980797063 2304 56-57 0.31579060473942633 2304 58-59 0.2622019929294481 2304 60-61 0.26987723368564787 2304 62-63 0.3650587254673283 2304 64-65 0.32916899122345455 2304 66-67 0.271031775268213 2304 68-69 0.19430625750201358 2304 70-71 -0.03189000306499423 2304 72-73 -0.15255069076524563 2304 74-75 -0.3763041645695182 2304 76-77 -0.365179092531843 2304 78-79 -0.1712567420137887 2304 80-81 -0.16717633840996982 2304 82-83 -0.3063229761252835 2304 84-85 -0.20974460832009356 2304 86-87 -0.3071339499583736 2304 88-89 -0.1290196634445131 2304 90-91 -0.4102618557665032 2304 92-93 -0.3088887257659181 2304 94-95 -0.18478448425153715 2304 96-97 -0.43169207754561967 2304 98-99 -0.4518577675259756 2304 100-101 -0.20381780553047335 2202 1 0.10281404714981335 2202 2 0.09094535236869916 2202 3 0.09232833029277288 2202 4 0.13063880183968735 2202 5 0.07248865759440548 2202 6 0.09120845107935338 2202 7 0.1340222991868245 2202 8 0.1289802160193858 2202 9 0.22419477118878461 2202 10-11 0.13429961252544587 2202 12-13 0.11173088899207784 2202 14-15 0.1474028963037881 2202 16-17 0.1892498030095524 2202 18-19 0.25349309520969143 2202 20-21 0.1281658606049163 2202 22-23 0.12187909998901603 2202 24-25 0.16121603078974545 2202 26-27 0.0800879717887355 2202 28-29 0.19912991059803886 2202 30-31 0.204753757015105 2202 32-33 0.18876338048825403 2202 34-35 0.14467478560070646 2202 36-37 0.3132315853819634 2202 38-39 0.35210418350833095 2202 40-41 0.3331892273527828 2202 42-43 0.3124229556251237 2202 44-45 0.37552848186259524 2202 46-47 0.5095295172844985 2202 48-49 0.36122479987930234 2202 50-51 0.5746225362066468 2202 52-53 0.43761367415098107 2202 54-55 0.47453760211021745 2202 56-57 0.3736157604198098 2202 58-59 0.3201821704040668 2202 60-61 0.4510825129376528 2202 62-63 0.516210547530676 2202 64-65 0.4936136788393384 2202 66-67 0.6082852348885837 2202 68-69 0.4963581178648653 2202 70-71 0.32795509672648393 2202 72-73 0.2268246220646759 2202 74-75 0.11504095852588847 2202 76-77 0.32586247154686987 2202 78-79 0.5231973279668978 2202 80-81 0.391960929842579 2202 82-83 0.3770561544962838 2202 84-85 0.6261957255137673 2202 86-87 0.515316448133543 2202 88-89 0.5051091444682818 2202 90-91 0.3324663049049761 2202 92-93 0.5403389009416024 2202 94-95 0.5533092340221906 2202 96-97 0.48680952029339863 2202 98-99 0.3343768037526367 2202 100-101 0.33485050659628257 1102 1 0.11925198025870287 1102 2 0.07282725045790528 1102 3 0.08020816387865182 1102 4 0.11816946342114676 1102 5 0.07416991670701378 1102 6 -0.01572363537464838 1102 7 0.10923067800656128 1102 8 0.07052375253484655 1102 9 0.12497131773319126 1102 10-11 0.06495673733794405 1102 12-13 0.029539724270385648 1102 14-15 -0.003193885262547269 1102 16-17 0.029771827422159447 1102 18-19 0.10336385250407432 1102 20-21 0.15345200583593765 1102 22-23 0.1594040510681367 1102 24-25 0.08324477737672709 1102 26-27 0.14195329170361504 1102 28-29 0.059950590194155495 1102 30-31 0.12077816253000861 1102 32-33 0.13761533782766122 1102 34-35 0.06509103079414302 1102 36-37 0.14626685502533832 1102 38-39 0.1836601740978594 1102 40-41 0.15428818422527257 1102 42-43 0.08636508847457236 1102 44-45 0.03633786311399234 1102 46-47 0.12123328041013792 1102 48-49 0.16920397985116864 1102 50-51 0.11543593252723383 1102 52-53 0.14003646720537688 1102 54-55 0.15469168481955364 1102 56-57 0.06481557648439207 1102 58-59 0.23769512261384307 1102 60-61 0.27480748315173287 1102 62-63 0.14321232391939986 1102 64-65 0.12541813761046683 1102 66-67 0.12594431599106315 1102 68-69 -0.04374896154982366 1102 70-71 0.027959103618343306 1102 72-73 0.07553719680888804 1102 74-75 0.008350444038253357 1102 76-77 0.015420552262028764 1102 78-79 0.15117942997146372 1102 80-81 0.15673252746668354 1102 82-83 0.1151862825341965 1102 84-85 0.16513385556003257 1102 86-87 0.11512498957311834 1102 88-89 0.1584968488708789 1102 90-91 0.017584844848098413 1102 92-93 0.050358992642184575 1102 94-95 0.008653242042516496 1102 96-97 -0.18514753836450026 1102 98-99 -0.14090585248411358 1102 100-101 0.004342509030944797 2305 1 0.06174336516826884 2305 2 0.020526994289774336 2305 3 0.0433767484490204 2305 4 0.0299863852569473 2305 5 0.06422313521832734 2305 6 -0.004501540455862596 2305 7 0.00382835058104547 2305 8 0.05635761566225739 2305 9 0.01764527279421202 2305 10-11 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Count 7 1.0 8 7.0 9 19.0 10 21.0 11 22.0 12 50.0 13 120.0 14 458.0 15 1234.0 16 2594.0 17 4381.0 18 5694.0 19 6867.0 20 8754.0 21 10985.0 22 14672.0 23 19141.0 24 24621.0 25 32058.0 26 43317.0 27 63862.0 28 102218.0 29 123696.0 30 83431.0 31 68866.0 32 89853.0 33 126567.0 34 192631.0 35 330954.0 36 633337.0 37 907741.0 38 197651.0 39 196.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.95634579833767 23.633111269612527 21.84249811173056 13.568044820319237 2 27.675831446770836 29.158315888888282 30.500911008621074 12.664941655719813 3 34.33690167922096 17.65670688713474 32.7134297302439 15.292961703400398 4 27.22529528840429 17.423364570818663 30.81727767075977 24.53406247001728 5 36.71469185862855 16.182969456856462 31.519495712874683 15.582842971640309 6 32.276352309207404 17.016982130923616 35.027207520367284 15.679458039501695 7 30.130144550146493 26.46404947773253 26.41860402019497 16.987201951926007 8 26.870313134383217 33.93990799152073 22.769498507599597 16.42028036649646 9 35.174267341382595 23.353538851021263 23.810964984388015 17.661228823208123 10-11 26.499482076821877 29.98696067433695 27.084346058599767 16.429211190241404 12-13 21.760008578758722 28.70100280392336 27.027046668641248 22.51194194867667 14-15 20.940633762260504 29.274513496202704 27.825313733539748 21.959539007997044 16-17 24.7343443305742 25.51378076168137 28.454686486097145 21.297188421647284 18-19 19.460038843430873 25.60003669228128 33.17356902525469 21.76635543903316 20-21 24.24744486387196 25.864569952574577 32.59456741060052 17.293417772952942 22-23 19.50700314130782 34.79275782472468 28.225565636472954 17.474673397494545 24-25 19.890301060813904 25.21187692969584 32.775493302851174 22.122328706639074 26-27 19.722165787742256 25.382967610986885 28.05462434177568 26.840242259495177 28-29 23.850195146049867 30.246531468118125 27.765276939109945 18.137996446722067 30-31 18.75204600030459 26.371637796697296 32.09701485426502 22.779301348733092 32-33 18.444691539263445 31.00678218753205 28.308119164302713 22.240407108901795 34-35 18.878944189456753 26.406455405505795 32.876363448438475 21.838236956598976 36-37 23.5085420889907 26.970630149446183 28.211261500456725 21.309566261106394 38-39 24.085411549459195 31.275914443090624 27.28706064157345 17.351613365876727 40-41 23.817308607105574 30.940423777120866 27.184000886456232 18.058266729317328 42-43 18.979466881538183 31.023512958752065 32.22849899872521 17.76852116098454 44-45 18.63955806672766 26.550703057308073 32.64923363402259 22.16050524194167 46-47 23.61483881230716 26.17284708264932 27.928288171601928 22.284025933441594 48-49 19.34316053601103 31.34416706276854 31.50548267980966 17.807189721410772 50-51 23.914929546165155 26.594206971221553 31.096427307095 18.394436175518294 52-53 18.928084340174966 26.524734353387036 26.826011563307784 27.721169743130208 54-55 23.885008160003594 26.52945346956439 31.184766614851224 18.400771755580795 56-57 19.284068507569057 26.60782279058438 31.02943238376334 23.078676318083225 58-59 19.180862056460867 26.474554746631334 31.676288738727028 22.668294458180775 60-61 23.292434413246824 26.731578322468508 31.687441896529585 18.288545367755088 62-63 18.86407328405242 26.81104448477521 31.313821563126304 23.011060668046067 64-65 23.522582477068323 26.612356284848143 31.15918445270295 18.705876785380582 66-67 19.43519370074568 35.29313221736382 26.434749288107817 18.836924793782682 68-69 19.4820669305873 35.68297340312695 26.35155385252445 18.4834058137613 70-71 18.881077417577668 36.54396921294782 26.27203792158317 18.302915447891348 72-73 18.749072122813377 36.273273663796274 26.191765862358356 18.785888351031993 74-75 19.48309124187771 34.97594272201302 26.152969746201816 19.38799628990745 76-77 19.80298581052602 33.825836496897985 26.57954888933154 19.791628803244453 78-79 19.640991580904657 33.26240429101862 27.19932794543966 19.89727618263706 80-81 19.059643496398365 33.36171700083697 27.14950558472347 20.429133918041188 82-83 18.531873256039873 32.77902917877355 27.31955074892675 21.369546816259827 84-85 18.719696592839142 32.06080288806612 27.793921649319365 21.42557886977538 86-87 18.846221132953385 31.920769476679805 28.388094453866596 20.84491493650021 88-89 19.209544229013165 32.27336599451655 27.905992135535136 20.61109764093515 90-91 19.664569363719757 31.752260995737824 27.49328174419279 21.089887896349634 92-93 19.13143133716951 32.17844263260411 27.510576293496808 21.179549736729573 94-95 18.90603758496601 32.544477158631494 27.46818848218289 21.081296774219606 96-97 19.344122934178202 32.93161109422863 26.83142997510925 20.89283599648392 98-99 19.88461002900257 32.106445277479025 26.50171569728128 21.507228996237128 100-101 19.75530208432612 32.06904461520816 26.190721574544177 21.984931725921534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1013.0 1 660.5 2 334.0 3 509.0 4 554.0 5 426.5 6 407.0 7 435.0 8 477.0 9 496.0 10 486.0 11 524.0 12 536.0 13 498.0 14 497.0 15 516.0 16 538.5 17 559.5 18 617.0 19 697.5 20 752.5 21 837.0 22 997.5 23 1216.0 24 1594.0 25 2285.5 26 3580.5 27 5782.5 28 8095.0 29 10199.0 30 12482.0 31 14693.5 32 18111.5 33 27595.0 34 44852.0 35 75324.0 36 115780.0 37 159977.5 38 209043.5 39 240959.0 40 251294.5 41 238190.0 42 214979.5 43 208352.0 44 198576.5 45 181295.5 46 171541.5 47 151478.5 48 117154.5 49 90390.5 50 71189.0 51 58292.5 52 48504.5 53 37437.5 54 28052.0 55 20267.0 56 13822.5 57 9028.5 58 6137.0 59 4418.0 60 3175.5 61 2284.0 62 1602.5 63 1108.5 64 804.0 65 589.5 66 433.5 67 320.5 68 228.5 69 169.0 70 124.5 71 88.0 72 68.5 73 50.0 74 34.0 75 23.0 76 17.0 77 16.0 78 11.5 79 6.0 80 5.0 81 4.0 82 4.0 83 3.5 84 3.0 85 2.5 86 2.5 87 3.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1474151159925052 2 0.0 3 0.0 4 0.015697578083338635 5 1.6149771690677608E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.006429224110059E-4 24-25 0.0 26-27 0.0 28-29 1.453479452160985E-4 30-31 0.001275831963563531 32-33 0.0013404310503262414 34-35 2.90695890432197E-4 36-37 0.0017764748859745371 38-39 0.00823638356224558 40-41 0.017684000001291982 42-43 0.00696055159868205 44-45 0.002470915068673674 46-47 5.329424657923611E-4 48-49 0.007251247489114247 50-51 0.0029392584477033246 52-53 0.011789333334194654 54-55 0.023853212787130828 56-57 0.021091601828024956 58-59 0.019977267581368204 60-61 0.02540359086943588 62-63 0.0074127452060210215 64-65 0.01148248767207178 66-67 0.02979632876930019 68-69 0.04119806758291858 70-71 0.03913089680651184 72-73 0.029602531509012056 74-75 0.03932469406679998 76-77 0.032735587217003516 78-79 0.01411490045765223 80-81 0.011046443836423486 82-83 0.005022578995800736 84-85 0.00791338812843203 86-87 0.024951397262096903 88-89 0.022722728768783396 90-91 0.017296405480715718 92-93 0.03254178995671538 94-95 0.0333169789978679 96-97 0.03535185023089329 98-99 0.025064445663931647 100-101 0.024999846577168937 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096019.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.71531993525777 #Duplication Level Percentage of deduplicated Percentage of total 1 86.22274476944202 32.51918404675579 2 6.09730535318985 4.599236442770302 3 2.1692791393154343 2.454451703044866 4 1.1058627195517319 1.6683186508947119 5 0.7566176798946933 1.4268038932950402 6 0.5407300713729525 1.2236284584267403 7 0.41597713484644755 1.0982097508539943 8 0.34180317786990216 1.0312972966600942 9 0.21738142747771516 0.7378749076774554 >10 1.6980003420436203 12.839274884563217 >50 0.2195957080384008 5.849702018585799 >100 0.18095600291622096 14.97928492477643 >500 0.027191434147369967 7.028239979141129 >1k 0.0061228730215197764 3.7557085939438144 >5k 3.457334986741355E-4 0.7717398181402477 >10k+ 8.643337343157102E-5 8.017044630470263 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 245932 7.943491302863451 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7279 0.2351083762728846 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 6013 0.19421715435208892 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5310 0.1715105753549962 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5072 0.16382328403023366 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 4801 0.1550701077738864 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4391 0.14182729498753077 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3508 0.1133067981817941 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3442 0.11117502831862466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004748032877059217 0.0 0.0 2 0.0051356273976354795 0.0 0.0 3 0.005910816438788005 0.0 0.0 4 0.006427609132889689 0.0 0.0 5 0.007719590868143897 0.0 0.0 6 0.008979273060016751 0.0 0.0 7 0.010465052055559091 0.0 0.0 8 0.012080029224626851 0.0 0.0 9 0.013759605480457323 0.0 0.0 10-11 0.023078023745978303 0.0 0.0 12-13 0.03194424840416031 0.0 0.0 14-15 0.04368513242328294 0.0 0.0 16-17 0.058058429227986 0.0 0.0 18-19 0.07369140822456192 0.0 0.0 20-21 0.0848832000062015 0.0 0.0 22-23 0.0948476091393496 0.0 0.0 24-25 0.10553875799857818 0.0 0.0 26-27 0.1176349369948957 0.0 0.0 28-29 0.13200823379959878 0.0 0.0 30-31 0.22680739362387634 0.0 0.0 32-33 0.24373235435570648 0.0 0.0 34-35 0.25739506120601974 0.0 0.0 36-37 0.27133231417507453 0.0 0.0 38-39 0.285786359838231 0.0 0.0 40-41 0.3014839379215696 0.0 0.0 42-43 0.3188933918041201 0.0 0.0 44-45 0.33518851144001377 0.0 0.0 46-47 0.35385764751443705 0.0 0.0 48-49 0.3747231525387925 0.0 0.0 50-51 0.3963315470609192 0.0 0.0 52-53 0.41945802012196953 0.0 0.0 54-55 0.4441994703520876 0.0 0.0 56-57 0.47239697172401074 0.0 0.0 58-59 0.5027100931874127 0.0 0.0 60-61 0.535251883144128 0.0 0.0 62-63 0.5724771068911398 0.0 0.0 64-65 0.612899985432906 0.0 0.0 66-67 0.6526284237919728 0.0 0.0 68-69 0.697056445713027 0.0 0.0 70-71 0.7450697169494115 0.0 0.0 72-73 0.797491875857351 0.0 0.0 74-75 0.8535800329390744 0.0 0.0 76-77 0.9150460639937934 0.0 0.0 78-79 0.9839571397979145 0.0 0.0 80-81 1.057987693227981 0.0 0.0 82-83 1.139269494147161 0.0 0.0 84-85 1.2271081023727568 0.0 0.0 86-87 1.3257024585443435 0.0 0.0 88-89 1.4282535087801462 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 34505 0.0 79.03648 4 TCGGAAG 34630 0.0 79.02549 3 GATCGGA 34860 0.0 78.78173 1 GAAGAGC 34685 0.0 78.49293 6 ATCGGAA 35020 0.0 78.26748 2 GGAAGAG 35690 0.0 76.54875 5 GAGCACA 42975 0.0 63.24092 9 AGAGCAC 43080 0.0 63.10883 8 AAGAGCA 43460 0.0 62.53517 7 TCGGACG 145 0.0 45.857086 3 GCCGTCT 29705 0.0 45.109608 50-51 ATGCCGT 29675 0.0 45.058807 48-49 CGTCTTC 29745 0.0 45.00902 52-53 TCGTATG 30125 0.0 44.81608 44-45 TCTCGTA 29360 0.0 44.737793 42-43 AATCTCG 29170 0.0 44.67423 40-41 CTGCTTG 30020 0.0 44.664337 58-59 GTATGCC 30155 0.0 44.59144 46-47 TGCCGTC 29765 0.0 44.547905 50-51 CCGTCTT 29775 0.0 44.51699 52-53 >>END_MODULE