FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3096019
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2459327.943491302863451TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72790.2351083762728846No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT60130.19421715435208892TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC53100.1715105753549962No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC50720.16382328403023366No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG48010.1550701077738864No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG43910.14182729498753077No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA35080.1133067981817941No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34420.11117502831862466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA345050.079.036484
TCGGAAG346300.079.025493
GATCGGA348600.078.781731
GAAGAGC346850.078.492936
ATCGGAA350200.078.267482
GGAAGAG356900.076.548755
GAGCACA429750.063.240929
AGAGCAC430800.063.108838
AAGAGCA434600.062.535177
TCGGACG1450.045.8570863
GCCGTCT297050.045.10960850-51
ATGCCGT296750.045.05880748-49
CGTCTTC297450.045.0090252-53
TCGTATG301250.044.8160844-45
TCTCGTA293600.044.73779342-43
AATCTCG291700.044.6742340-41
CTGCTTG300200.044.66433758-59
GTATGCC301550.044.5914446-47
TGCCGTC297650.044.54790550-51
CCGTCTT297750.044.5169952-53
Produced by FastQC (version 0.11.2)