##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843261 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65049605020667 33.0 33.0 33.0 33.0 33.0 2 32.67344776458678 33.0 33.0 33.0 33.0 33.0 3 32.70110147179374 33.0 33.0 33.0 33.0 33.0 4 36.63245411257549 37.0 37.0 37.0 37.0 37.0 5 36.64386269768633 37.0 37.0 37.0 37.0 37.0 6 36.51064716282718 37.0 37.0 37.0 37.0 37.0 7 36.428489508539485 37.0 37.0 37.0 37.0 37.0 8 36.33482887122334 37.0 37.0 37.0 37.0 37.0 9 36.37987675104068 37.0 37.0 37.0 37.0 37.0 10-11 36.31158419778859 37.0 37.0 37.0 37.0 37.0 12-13 36.28827333730817 37.0 37.0 37.0 35.0 37.0 14-15 38.08623846541537 40.0 37.0 40.0 35.0 40.0 16-17 38.00694665595377 40.0 37.0 40.0 35.0 40.0 18-19 37.89758883847703 40.0 37.0 40.0 33.0 40.0 20-21 37.83093712718926 40.0 37.0 40.0 33.0 40.0 22-23 37.62546269898837 40.0 37.0 40.0 33.0 40.0 24-25 37.59614455033769 40.0 37.0 40.0 33.0 40.0 26-27 37.463658158014525 40.0 37.0 40.0 33.0 40.0 28-29 37.24538060535106 40.0 37.0 40.0 33.0 40.0 30-31 37.14604524264334 40.0 37.0 40.0 33.0 40.0 32-33 36.97861941417954 37.0 37.0 40.0 33.0 40.0 34-35 36.814116666060855 37.0 37.0 40.0 33.0 40.0 36-37 36.673718209195556 37.0 37.0 40.0 33.0 40.0 38-39 36.52503959016113 37.0 37.0 40.0 33.0 40.0 40-41 36.39253448100947 37.0 37.0 40.0 33.0 40.0 42-43 36.044055616648976 37.0 37.0 40.0 33.0 40.0 44-45 35.8134496416948 37.0 37.0 40.0 30.0 40.0 46-47 35.77938854020131 37.0 37.0 40.0 30.0 40.0 48-49 35.497593395617876 37.0 35.0 40.0 27.0 40.0 50-51 35.318009223870085 37.0 33.0 40.0 27.0 40.0 52-53 35.12182837916063 37.0 33.0 40.0 27.0 40.0 54-55 34.78930845930121 37.0 33.0 40.0 27.0 40.0 56-57 34.52903522615625 37.0 33.0 37.0 27.0 40.0 58-59 34.29043553788638 37.0 33.0 37.0 27.0 40.0 60-61 33.98647478571944 37.0 33.0 37.0 27.0 40.0 62-63 33.658025097910716 37.0 33.0 37.0 27.0 40.0 64-65 33.38292352520885 37.0 33.0 37.0 27.0 38.5 66-67 32.89294326739403 37.0 33.0 37.0 22.0 37.0 68-69 32.59599644326007 37.0 33.0 37.0 22.0 37.0 70-71 32.233603651354855 33.0 33.0 37.0 22.0 37.0 72-73 31.720712367917514 33.0 33.0 37.0 22.0 37.0 74-75 31.141924285274847 33.0 27.0 37.0 15.0 37.0 76-77 28.35786304815216 30.0 24.5 33.0 15.0 35.0 78-79 30.901948503223366 33.0 30.0 37.0 15.0 37.0 80-81 31.38999577379438 33.0 33.0 37.0 15.0 37.0 82-83 31.318562048456513 33.0 33.0 37.0 15.0 37.0 84-85 31.208333762825774 33.0 33.0 37.0 15.0 37.0 86-87 31.025124222776917 33.0 33.0 37.0 15.0 37.0 88-89 30.892639729262434 33.0 33.0 37.0 15.0 37.0 90-91 30.760350270525983 33.0 30.0 37.0 15.0 37.0 92-93 30.54591482161235 33.0 27.0 37.0 15.0 37.0 94-95 30.394503545618335 33.0 27.0 37.0 15.0 37.0 96-97 30.194733952489635 33.0 27.0 37.0 15.0 37.0 98-99 30.07680599763137 33.0 27.0 37.0 15.0 37.0 100-101 28.32596062087789 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13707220358791972 2306 2 0.19918050310131719 2306 3 0.19034497583583487 2306 4 0.20782701293438777 2306 5 0.18390243358668812 2306 6 0.2102945842953119 2306 7 0.28778737001200483 2306 8 0.18029706823237746 2306 9 0.20996973260345442 2306 10-11 0.23954939625231475 2306 12-13 0.2964138360756081 2306 14-15 0.5265788361664434 2306 16-17 0.5489746820422567 2306 18-19 0.6187181116810692 2306 20-21 0.6624354253126725 2306 22-23 0.6346699703107177 2306 24-25 0.6006506623458918 2306 26-27 0.7088692145740296 2306 28-29 0.7219828087383604 2306 30-31 0.8258038945833022 2306 32-33 0.807777626042423 2306 34-35 0.7709524169233006 2306 36-37 0.8211934303528423 2306 38-39 0.94241458133218 2306 40-41 0.9456664536355817 2306 42-43 0.9141706040554638 2306 44-45 1.0809035803486822 2306 46-47 1.0560963945153006 2306 48-49 1.0372833499840652 2306 50-51 1.1300824645786847 2306 52-53 1.0492792703468865 2306 54-55 1.0776901955068396 2306 56-57 1.0347512025413792 2306 58-59 0.9494971825334702 2306 60-61 0.9634060776413165 2306 62-63 1.0774929761686778 2306 64-65 0.9194397000304306 2306 66-67 1.130506518633105 2306 68-69 1.2362765863958813 2306 70-71 1.007922800026087 2306 72-73 0.899184410395268 2306 74-75 0.786780943852488 2306 76-77 0.3104786303480296 2306 78-79 0.8682455838588545 2306 80-81 1.0517948039753442 2306 82-83 1.0840869894184877 2306 84-85 1.0137493540247249 2306 86-87 1.0806331766403616 2306 88-89 1.0679761817492697 2306 90-91 0.8726260154965821 2306 92-93 0.734904055864849 2306 94-95 0.9929732632672916 2306 96-97 0.901455784829281 2306 98-99 0.9560136328201772 2306 100-101 0.730426662797921 2307 1 0.09940718415821692 2307 2 0.1228667798726164 2307 3 0.17095297484216587 2307 4 0.11144368876880151 2307 5 0.087265148067587 2307 6 0.15039630191690634 2307 7 0.1292478363249785 2307 8 0.24860158231226137 2307 9 0.12324470288221079 2307 10-11 0.24512490151341382 2307 12-13 0.2664646948864018 2307 14-15 0.4702432212472729 2307 16-17 0.4470269426310125 2307 18-19 0.6134475818444898 2307 20-21 0.5767062789777029 2307 22-23 0.5765050573451518 2307 24-25 0.6008796711646767 2307 26-27 0.5913089514301433 2307 28-29 0.5917422219434627 2307 30-31 0.6179171192049111 2307 32-33 0.6158066863188765 2307 34-35 0.5065653398964898 2307 36-37 0.5912696852989825 2307 38-39 0.6054289362481668 2307 40-41 0.5522991700683235 2307 42-43 0.5865416049958938 2307 44-45 0.8160346421511164 2307 46-47 0.8278106447415396 2307 48-49 0.7843078988422434 2307 50-51 0.6437869511553274 2307 52-53 0.7710019489848534 2307 54-55 0.7711283442727392 2307 56-57 0.8626380444598567 2307 58-59 0.938038424082869 2307 60-61 0.6225555621774035 2307 62-63 0.7692387885788534 2307 64-65 0.8959538198380912 2307 66-67 0.8929905490108823 2307 68-69 0.8912168555727362 2307 70-71 0.5465293617664173 2307 72-73 0.6348932620019845 2307 74-75 0.6374899285393987 2307 76-77 0.5374075167330368 2307 78-79 0.9318745474303753 2307 80-81 1.0130063659818411 2307 82-83 1.0470435653540449 2307 84-85 0.9330916717492244 2307 86-87 0.9556919537221802 2307 88-89 0.8299522894200138 2307 90-91 0.6970560996032624 2307 92-93 0.6469308449061373 2307 94-95 0.7492302499770176 2307 96-97 0.8827275212595396 2307 98-99 0.9254029722101365 2307 100-101 0.6786046841617228 2201 1 0.20538056098445168 2201 2 0.15917544153273155 2201 3 0.15178992920301937 2201 4 0.15961349771649225 2201 5 0.18300836143029642 2201 6 0.1446290295142063 2201 7 0.26012921673775224 2201 8 0.18089242486552592 2201 9 0.19440955802082271 2201 10-11 0.2264380666363266 2201 12-13 0.23327802838202416 2201 14-15 0.3835469980702584 2201 16-17 0.4479144493760572 2201 18-19 0.5378522930345824 2201 20-21 0.5887875279380523 2201 22-23 0.5759806676455028 2201 24-25 0.5732072108539796 2201 26-27 0.6192638924591236 2201 28-29 0.6647175908419811 2201 30-31 0.524209085595281 2201 32-33 0.6523283495978021 2201 34-35 0.6457304546942169 2201 36-37 0.6374854579674292 2201 38-39 0.769740413586824 2201 40-41 0.7166512191438983 2201 42-43 0.7599441942841452 2201 44-45 0.981872123944548 2201 46-47 1.016645201312734 2201 48-49 0.9076221431733558 2201 50-51 1.0128355844963721 2201 52-53 0.8028680054526021 2201 54-55 0.8052025175221686 2201 56-57 0.8824674808778639 2201 58-59 0.8598607442182171 2201 60-61 0.9229446451344288 2201 62-63 0.9777790377704534 2201 64-65 0.8859265150591469 2201 66-67 1.0140053901522421 2201 68-69 0.8250438455150046 2201 70-71 0.8046178446674546 2201 72-73 0.7676987570380369 2201 74-75 0.6783233531591399 2201 76-77 0.427352116026281 2201 78-79 0.9289574395460711 2201 80-81 0.901756634769594 2201 82-83 0.999196808861555 2201 84-85 0.8939810245083066 2201 86-87 0.9994095216899836 2201 88-89 0.7726979614299765 2201 90-91 0.7678608805767375 2201 92-93 0.6633938414534839 2201 94-95 0.6731070214404795 2201 96-97 0.8026566627210165 2201 98-99 0.8084564619880865 2201 100-101 0.6360647108579265 1101 1 0.05410232406985216 1101 2 0.07804596494870708 1101 3 0.07386706418924405 1101 4 0.1568752057054681 1101 5 0.08345464242203349 1101 6 0.13089900196600013 1101 7 0.06789781177907628 1101 8 0.13688140558177508 1101 9 0.1782167205552625 1101 10-11 0.10160060107158841 1101 12-13 0.14204937824428754 1101 14-15 0.17745333415841458 1101 16-17 0.24547367802620101 1101 18-19 0.22080546107865473 1101 20-21 0.21542940121629073 1101 22-23 0.32073824340308477 1101 24-25 0.29035046154267974 1101 26-27 0.2833079695941123 1101 28-29 0.27309224648936237 1101 30-31 0.26979586245479226 1101 32-33 0.3212264212231446 1101 34-35 0.46536406350964654 1101 36-37 0.5136452375817555 1101 38-39 0.32386438052896693 1101 40-41 0.322240750824335 1101 42-43 0.2488312466257483 1101 44-45 0.3738363112723775 1101 46-47 0.4110281214229303 1101 48-49 0.5435945949639915 1101 50-51 0.5091055569481568 1101 52-53 0.32292585669226526 1101 54-55 0.5616570629767139 1101 56-57 0.36109558005141906 1101 58-59 0.6281307166700145 1101 60-61 0.2619060776413136 1101 62-63 0.2061867512690796 1101 64-65 0.17664552332360728 1101 66-67 0.38361696040018245 1101 68-69 0.35023943780150546 1101 70-71 0.3335131614718634 1101 72-73 0.07093842645952009 1101 74-75 0.15987431734646762 1101 76-77 0.10812622070947597 1101 78-79 0.3928500015295384 1101 80-81 0.29197151080265726 1101 82-83 0.3242546601012215 1101 84-85 0.38197826968737303 1101 86-87 0.5179604858773104 1101 88-89 0.6844279889781859 1101 90-91 0.4628278227254974 1101 92-93 0.3979833731339255 1101 94-95 0.1525395283275337 1101 96-97 0.14935638723892453 1101 98-99 0.13670439587238548 1101 100-101 0.34028509653285965 2304 1 0.12200131396883762 2304 2 0.12484731175005237 2304 3 0.08728120560338937 2304 4 0.1307337915640545 2304 5 0.10844454141192728 2304 6 0.08442088748788024 2304 7 0.12099545487672003 2304 8 0.1809689476693208 2304 9 0.14179247813101625 2304 10-11 0.09732113979212897 2304 12-13 0.06024466443957266 2304 14-15 0.15843986614494554 2304 16-17 0.22673293728546184 2304 18-19 0.3491623634355605 2304 20-21 0.39842940121628345 2304 22-23 0.31806820973304895 2304 24-25 0.43071409790631776 2304 26-27 0.282594164880301 2304 28-29 0.3764255798227012 2304 30-31 0.49458710824603 2304 32-33 0.4848964548931818 2304 34-35 0.38518561233119897 2304 36-37 0.46348025441677265 2304 38-39 0.42119434685893253 2304 40-41 0.5126414242250092 2304 42-43 0.5693564991509916 2304 44-45 0.6277016311376897 2304 46-47 0.6038429362377471 2304 48-49 0.5852545276239169 2304 50-51 0.6625601024027077 2304 52-53 0.640134610901022 2304 54-55 0.6666503289699861 2304 56-57 0.7442672972231321 2304 58-59 0.6954438479831424 2304 60-61 0.5790205557557968 2304 62-63 0.64186688594922 2304 64-65 0.707153940832022 2304 66-67 0.675919990703207 2304 68-69 0.6329027374648035 2304 70-71 0.3928868988456031 2304 72-73 0.36624482376591416 2304 74-75 0.2913322298043788 2304 76-77 0.24121039579364734 2304 78-79 0.5476749173544562 2304 80-81 0.5543890192201637 2304 82-83 0.4941233469699071 2304 84-85 0.5818368555459585 2304 86-87 0.5697416306584557 2304 88-89 0.6375357330859295 2304 90-91 0.30277058366825926 2304 92-93 0.4440641812147348 2304 94-95 0.49068430947231434 2304 96-97 0.30688837377091005 2304 98-99 0.33333402550201185 2304 100-101 0.19118408643184992 2202 1 0.05943113344677897 2202 2 0.09176177823268716 2202 3 0.06019978061943476 2202 4 0.09626323778443435 2202 5 -0.017483461628970076 2202 6 0.08802094315456088 2202 7 0.08435473137603111 2202 8 0.12315037535352502 2202 9 0.15361832450344082 2202 10-11 0.029667843514523895 2202 12-13 0.07812093900924566 2202 14-15 0.016171615054979327 2202 16-17 -0.05197933451749748 2202 18-19 -0.027531368039170445 2202 20-21 0.1354435899886468 2202 22-23 0.037097075399792345 2202 24-25 0.0038096431178402668 2202 26-27 -0.08303955251158612 2202 28-29 -0.008028460892909095 2202 30-31 0.03783554989053073 2202 32-33 -0.05586796495822455 2202 34-35 0.003092420490929726 2202 36-37 0.07545461450957447 2202 38-39 0.01096143995730614 2202 40-41 -0.01753490206482411 2202 42-43 0.15275557317314536 2202 44-45 0.12262142334311221 2202 46-47 0.31421833322634285 2202 48-49 0.18481750263414654 2202 50-51 0.16489498734092933 2202 52-53 0.08201880343296608 2202 54-55 -0.13129851999675424 2202 56-57 0.05043508241621453 2202 58-59 0.2785251234148163 2202 60-61 0.3935032398868472 2202 62-63 0.22910737639760015 2202 64-65 0.1753539337698342 2202 66-67 0.22850817981206006 2202 68-69 0.1490732852207941 2202 70-71 0.1240511010153611 2202 72-73 0.046195263802726316 2202 74-75 -0.006134936200727026 2202 76-77 0.09542912015426097 2202 78-79 0.32609223883161675 2202 80-81 0.3596461972102212 2202 82-83 0.3320952934551187 2202 84-85 0.5547074492061874 2202 86-87 0.5770065479788933 2202 88-89 0.6069381678800987 2202 90-91 0.5061091305601693 2202 92-93 0.4970156577319109 2202 94-95 0.598990484527409 2202 96-97 0.300862247818813 2202 98-99 0.1766429111921468 2202 100-101 0.49579465853162574 1102 1 0.08664245295092599 1102 2 0.06240448867454518 1102 3 0.0436995187879603 1102 4 0.07012418051508007 1102 5 0.00535388085202726 1102 6 -0.011294319650872353 1102 7 -0.07816545711371958 1102 8 0.07121883967668197 1102 9 0.07044987814167314 1102 10-11 -0.07221369301159797 1102 12-13 0.03228019253837289 1102 14-15 0.01678725331483122 1102 16-17 0.04954749173445805 1102 18-19 0.22987517402534507 1102 20-21 -0.030744588238896142 1102 22-23 0.13123443555306125 1102 24-25 0.11590875093928332 1102 26-27 0.10130914124027157 1102 28-29 0.12692352944191754 1102 30-31 0.1391883639193523 1102 32-33 0.21016022321494887 1102 34-35 0.13261479578849844 1102 36-37 0.3151865381089962 1102 38-39 0.2136224355963421 1102 40-41 0.22852018843417454 1102 42-43 0.20517336730529934 1102 44-45 0.1825873364627668 1102 46-47 0.30367369845867387 1102 48-49 0.1053186722926327 1102 50-51 0.17047403966638086 1102 52-53 0.009768856106440182 1102 54-55 0.1577748134160828 1102 56-57 0.2605460780010418 1102 58-59 0.17772181215917016 1102 60-61 0.253278661121108 1102 62-63 -0.016656248145096697 1102 64-65 0.09474218413204483 1102 66-67 0.18422270146637487 1102 68-69 0.02720546006278113 1102 70-71 0.035712341319552365 1102 72-73 -0.06252027301323793 1102 74-75 0.007052407563222118 1102 76-77 0.28698152240836095 1102 78-79 0.1458511731757035 1102 80-81 0.0902597357587176 1102 82-83 0.12342337714867213 1102 84-85 0.1256794999158437 1102 86-87 0.2439997360237669 1102 88-89 0.1272885630848073 1102 90-91 0.09994147240329809 1102 92-93 0.0433061616517918 1102 94-95 0.14340010243415335 1102 96-97 -0.12293593847871165 1102 98-99 0.04705530389816204 1102 100-101 0.1475651199657797 2305 1 0.03576492118416752 2305 2 -0.04544645088554944 2305 3 0.011719820386062452 2305 4 0.009137136893038189 2305 5 0.0675382532248392 2305 6 0.009405846213127234 2305 7 0.001834178778707951 2305 8 0.01235532345081225 2305 9 -0.07782545480544911 2305 10-11 -0.018147456642061854 2305 12-13 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-0.09344714098559592 1213 72-73 0.08809406603104009 1213 74-75 -0.1353033764153473 1213 76-77 -0.07565071690867242 1213 78-79 -0.46688975902496566 1213 80-81 -0.6295168319824747 1213 82-83 -0.44687262408277206 1213 84-85 -0.7641351518627246 1213 86-87 -0.8800174599323967 1213 88-89 -0.7143224836116069 1213 90-91 -0.4640322906960499 1213 92-93 -0.3520443521338734 1213 94-95 -0.5846003582509169 1213 96-97 -0.558093518243119 1213 98-99 -0.45710215737273074 1213 100-101 -0.02461282407205445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 4.0 10 11.0 11 10.0 12 50.0 13 177.0 14 751.0 15 2221.0 16 4561.0 17 7045.0 18 7191.0 19 6877.0 20 7535.0 21 8653.0 22 10052.0 23 12267.0 24 14665.0 25 18400.0 26 23108.0 27 30814.0 28 45241.0 29 63259.0 30 59124.0 31 58886.0 32 77774.0 33 110275.0 34 166771.0 35 271426.0 36 419250.0 37 370531.0 38 46312.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.25809763833256 23.13777814961881 21.954907151716395 12.649217060332235 2 33.2470008316782 23.774224051829883 30.337537657445147 12.641237459046764 3 38.363639224179316 15.857276858784514 29.14476029167872 16.63432362535745 4 33.189146015703976 15.38307940485292 31.006639815124803 20.421134764318303 5 33.771108672194195 14.50946394402523 35.10304303410427 16.61638434967631 6 30.053421626128912 14.802570010432598 38.48038883261785 16.663619530820647 7 35.648722562892615 19.494146515333423 26.628242012389997 18.22888890938397 8 32.6146432870874 29.328781979329026 21.14035939565802 16.916215337925557 9 34.42854810035041 24.80402938053808 21.891528112405133 18.875894406706376 10-11 27.646166223882563 25.899479238154555 27.601978233142237 18.85237630482064 12-13 25.487329249628782 24.72604259516151 26.214084711823233 23.572543443386476 14-15 24.825865680443517 26.031473567769297 26.00602953135774 23.136631220429447 16-17 26.31558959908553 24.686086235210315 26.9317801439948 22.06654402170935 18-19 23.788058229409724 24.42380650379952 28.924254351391365 22.863880915399392 20-21 25.367731428159118 24.98539273602599 29.205006778747016 20.441869057067883 22-23 23.713390869201024 29.81145863623935 25.755999166691986 20.719151327867642 24-25 23.74883426709511 24.407531000764408 28.54077637404578 23.302858358094703 26-27 22.670419435988716 24.783115359137962 26.487865798712175 26.058599406161147 28-29 24.845057127046648 27.097370962316763 25.98778794093074 22.069783969705846 30-31 22.418376943481995 25.22931710904332 28.56842920187745 23.783876745597233 32-33 22.840901322992156 27.219427032528486 25.910594679169247 24.02907696531011 34-35 23.16130745562413 26.88948571809884 28.39082071732168 21.558386108955357 36-37 22.63023105862605 27.686996791236705 28.709818913338975 20.97295323679827 38-39 22.62482682251081 26.006911636686308 27.44390161592536 23.924359924877525 40-41 25.37220380846083 25.40901927345209 24.864031019403136 24.354745898683948 42-43 23.207023816467125 27.401754869022316 27.39079541493334 22.00042589957722 44-45 22.674825184309906 25.139008922937865 27.32967778792303 24.856488104829193 46-47 25.142987657422054 25.0494573860839 25.417719266503497 24.38983568999055 48-49 22.858615493334092 27.169773444610374 27.281376015755658 22.690235046299875 50-51 25.005045523643975 24.825658169570826 26.465643239161075 23.703653067624124 52-53 22.739354775701543 24.937767215747552 24.549693990494173 27.773184018056728 54-55 25.203943121933936 24.88072172816682 27.366593510231002 22.548741639668243 56-57 22.869582864417097 24.93076137526575 27.049430734786768 25.150225025530386 58-59 22.45660062536881 25.09573840320688 27.351108639178474 25.096552332245835 60-61 24.479558109386268 25.09788046400902 27.437216770701685 22.985344655903024 62-63 22.65262673591142 24.951142733749872 27.513477118197148 24.882753412141557 64-65 25.25577223791484 25.54317689303468 27.024257669097786 22.176793199952687 66-67 22.998655812593032 29.589379779883206 24.768321675930906 22.643642731592852 68-69 22.60739121397159 28.63556755108033 25.51929701352319 23.23774422142489 70-71 22.625288252121628 29.092555999198943 25.216504547178914 23.06565120150051 72-73 22.896022041073042 29.27496884397513 25.17632017295969 22.652688941992135 74-75 23.497937261152803 28.618924228029858 25.326954215419782 22.556184295397554 76-77 23.508989383250626 27.874427844119083 25.22486685987937 23.391715912750925 78-79 23.279088347840712 27.952512916926093 25.453147063730736 23.31525167150246 80-81 23.522720552209986 28.22759563271438 25.34045154082841 22.90923227424722 82-83 23.01903375837042 27.875470147259684 25.559490498040745 23.546005596329156 84-85 22.85433319181663 28.034821248123095 25.334953767441483 23.775891792618797 86-87 22.534834472663537 28.228736082829464 25.46726556681395 23.76916387769305 88-89 22.540167207583433 28.364975291110174 25.68579582051584 23.409061680790554 90-91 22.98939262481124 27.915590275008235 25.20685439696836 23.888162703212164 92-93 22.712731404978857 27.57589225874269 24.902901214569187 24.808475121709264 94-95 22.891026844683324 28.112351940712248 24.504090251449796 24.492530963154632 96-97 22.738755713926757 28.840846890370216 24.269759753224573 24.150637642478458 98-99 22.88708519923867 27.656115398755443 24.803732308097203 24.653067093908682 100-101 23.50505013780503 27.85617810953967 24.3294112667481 24.309360485907206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3533.0 1 2118.0 2 785.5 3 2214.0 4 2714.0 5 1513.5 6 1327.0 7 1587.0 8 1716.5 9 1677.5 10 1488.5 11 1339.5 12 1296.5 13 1251.0 14 1211.0 15 1176.0 16 1168.5 17 1227.5 18 1245.0 19 1301.5 20 1376.5 21 1429.5 22 1518.0 23 1635.5 24 1787.5 25 1918.5 26 2164.0 27 2531.5 28 2927.0 29 3449.0 30 4139.5 31 5180.5 32 6452.5 33 8094.0 34 11058.5 35 16185.0 36 22935.5 37 31847.5 38 41275.0 39 47081.0 40 54088.5 41 58929.5 42 63836.5 43 74578.0 44 79641.0 45 82708.5 46 92816.0 47 98809.5 48 95170.5 49 91156.5 50 88308.5 51 86220.0 52 83680.0 53 80607.0 54 75295.0 55 65939.5 56 56912.0 57 50641.5 58 46730.0 59 40478.5 60 31512.5 61 23346.0 62 16417.0 63 11522.0 64 8665.0 65 7111.0 66 6630.0 67 6117.0 68 5140.5 69 3958.0 70 2754.5 71 1954.0 72 1710.5 73 1402.5 74 902.0 75 558.0 76 417.0 77 334.0 78 273.5 79 217.0 80 162.5 81 127.0 82 95.0 83 74.0 84 58.0 85 42.0 86 23.5 87 12.5 88 19.0 89 15.5 90 9.5 91 7.0 92 4.0 93 4.0 94 4.0 95 3.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1394268093341095 2 0.0 3 0.0 4 0.015624482913705655 5 1.0850335356740037E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.7125838391850096E-4 24-25 0.0 26-27 0.0 28-29 5.425167678370019E-5 30-31 8.951526669310532E-4 32-33 8.137751517555028E-4 34-35 8.13775151755503E-5 36-37 0.0014919211115517552 38-39 0.007459605557758777 40-41 0.015488853721746406 42-43 0.00561504854711297 44-45 0.0018445570106458064 46-47 4.068875758777514E-4 48-49 0.006157565314949972 50-51 0.0023328221016991084 52-53 0.009819553497849735 54-55 0.02205330661257413 56-57 0.01947635196534837 58-59 0.018906709359119516 60-61 0.02452175790623249 62-63 0.007432479719366926 64-65 0.011664110508495541 66-67 0.027776858513254496 68-69 0.03916971063783154 70-71 0.03743365698075313 72-73 0.02818374608913225 74-75 0.037243776112010185 76-77 0.030353813160480258 78-79 0.012667766528993995 80-81 0.010524825296037836 82-83 0.004801273395357466 84-85 0.007242598850623975 86-87 0.023789360269652535 88-89 0.02180917406704748 90-91 0.016166999681542657 92-93 0.029838422231035108 94-95 0.031845734272032016 96-97 0.03331052954519192 98-99 0.023518101885734034 100-101 0.023870737784828084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1843261.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.5027160938687 #Duplication Level Percentage of deduplicated Percentage of total 1 81.70514132286098 37.99510990346423 2 8.538688934310027 7.9414445465215495 3 2.7894152051168577 3.8914615005440925 4 1.6357855817825717 3.042738900003151 5 1.1148031419073838 2.592068700433594 6 0.7305601211017871 2.0383817940659243 7 0.5897781145188513 1.9198398952493128 8 0.32369669754139657 1.2042220501032361 9 0.35312323720274386 1.4779070681208344 >10 1.9601048454853207 17.725183826336917 >50 0.15667933806848175 4.969215104871141 >100 0.09694242999083089 8.056351164793139 >500 0.004005259633696897 1.2602022149432668 >1k 0.001043941035596652 1.1926578213507517 >5k 0.0 0.0 >10k+ 2.3182944350129986E-4 4.693215509198896 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 72349 3.9250545636239256 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14718 0.7984761789024994 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4273 0.2318174148967509 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4034 0.21885126414544656 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3824 0.20745841202086954 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2422 0.13139756117012188 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2000 0.10850335356740039 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0026040804856176094 0.0 0.0 2 0.0029838422231035105 0.0 0.0 3 0.003363603960589412 0.0 0.0 4 0.0035263589909405126 0.0 0.0 5 0.004557140849830816 0.0 0.0 6 0.005099657617667818 0.0 0.0 7 0.005913432769423321 0.0 0.0 8 0.006672956244395124 0.0 0.0 9 0.007920744810420228 0.0 0.0 10-11 0.01714352986364926 0.0 0.0 12-13 0.03154735004972166 0.0 0.0 14-15 0.050399807732057475 0.0 0.0 16-17 0.07996697157917408 0.0 0.0 18-19 0.11764476110545387 0.0 0.0 20-21 0.12944450080590864 0.0 0.0 22-23 0.13603607953512822 0.0 0.0 24-25 0.144580718628561 0.0 0.0 26-27 0.15431889461123519 0.0 0.0 28-29 0.16557611754385299 0.0 0.0 30-31 0.2210755828935783 0.0 0.0 32-33 0.23235993166458793 0.0 0.0 34-35 0.2411215774651555 0.0 0.0 36-37 0.24985609742733123 0.0 0.0 38-39 0.25856349155111513 0.0 0.0 40-41 0.2653178253106858 0.0 0.0 42-43 0.273781086888943 0.0 0.0 44-45 0.2831123752957394 0.0 0.0 46-47 0.2937999556221284 0.0 0.0 48-49 0.3035923832815863 0.0 0.0 50-51 0.315582003850784 0.0 0.0 52-53 0.3275987502583736 0.0 0.0 54-55 0.3407547818784209 0.0 0.0 56-57 0.3562707614385592 0.0 0.0 58-59 0.3723835094433181 0.0 0.0 60-61 0.39172423221670727 0.0 0.0 62-63 0.412394121071297 0.0 0.0 64-65 0.43341664582498085 0.0 0.0 66-67 0.45772139702407855 0.0 0.0 68-69 0.4837079502034709 0.0 0.0 70-71 0.511376305363158 0.0 0.0 72-73 0.5442528214940803 0.0 0.0 74-75 0.584670320697937 0.0 0.0 76-77 0.6283971721855993 0.0 0.0 78-79 0.6790953641399671 0.0 0.0 80-81 0.7345948294896925 0.0 0.0 82-83 0.7903655532233362 0.0 0.0 84-85 0.8588582951627577 0.0 0.0 86-87 0.9341867483769255 0.0 0.0 88-89 1.0145063558551937 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 10780 0.0 70.88931 4 ATCGGAA 10885 0.0 70.42367 2 GATCGGA 10990 0.0 69.975395 1 TCGGAAG 11015 0.0 69.764984 3 GAAGAGC 11165 0.0 68.47811 6 GGAAGAG 13205 0.0 58.042984 5 GAGCACA 14685 0.0 52.257908 9 AAGAGCA 14985 0.0 51.02156 7 AGAGCAC 15495 0.0 49.648716 8 TATGCCG 8955 0.0 42.355114 48-49 CTCGTAT 8640 0.0 42.215065 44-45 GTATGCC 9025 0.0 42.15189 46-47 TACTCGA 8980 0.0 41.998596 36-37 GATCTCG 8670 0.0 41.989098 40-41 GCCGTCT 9215 0.0 41.520332 50-51 TCGTATG 9210 0.0 41.432983 44-45 TCGATCT 8795 0.0 41.36757 38-39 ATGCCGT 9175 0.0 41.3654 48-49 CTCGATC 8775 0.0 41.353596 38-39 CTGCTTG 9280 0.0 41.109356 58-59 >>END_MODULE