FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843261
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT723493.9250545636239256TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147180.7984761789024994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42730.2318174148967509No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA40340.21885126414544656No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC38240.20745841202086954No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT24220.13139756117012188TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.10850335356740039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA107800.070.889314
ATCGGAA108850.070.423672
GATCGGA109900.069.9753951
TCGGAAG110150.069.7649843
GAAGAGC111650.068.478116
GGAAGAG132050.058.0429845
GAGCACA146850.052.2579089
AAGAGCA149850.051.021567
AGAGCAC154950.049.6487168
TATGCCG89550.042.35511448-49
CTCGTAT86400.042.21506544-45
GTATGCC90250.042.1518946-47
TACTCGA89800.041.99859636-37
GATCTCG86700.041.98909840-41
GCCGTCT92150.041.52033250-51
TCGTATG92100.041.43298344-45
TCGATCT87950.041.3675738-39
ATGCCGT91750.041.365448-49
CTCGATC87750.041.35359638-39
CTGCTTG92800.041.10935658-59
Produced by FastQC (version 0.11.2)