##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7679010 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.670999907540164 33.0 33.0 33.0 33.0 33.0 2 32.702036720879384 33.0 33.0 33.0 33.0 33.0 3 32.72827408741492 33.0 33.0 33.0 33.0 33.0 4 36.66191071505311 37.0 37.0 37.0 37.0 37.0 5 36.66376915774299 37.0 37.0 37.0 37.0 37.0 6 36.53354989770817 37.0 37.0 37.0 37.0 37.0 7 36.46459126892659 37.0 37.0 37.0 37.0 37.0 8 36.388672758597785 37.0 37.0 37.0 37.0 37.0 9 36.423134101921995 37.0 37.0 37.0 37.0 37.0 10-11 36.37369446061406 37.0 37.0 37.0 37.0 37.0 12-13 36.34492304346524 37.0 37.0 37.0 37.0 37.0 14-15 38.19688976834253 40.0 37.0 40.0 37.0 40.0 16-17 38.158458642455216 40.0 37.0 40.0 37.0 40.0 18-19 38.12391304347826 40.0 37.0 40.0 37.0 40.0 20-21 38.06245381891676 40.0 37.0 40.0 37.0 40.0 22-23 37.923938697826934 40.0 37.0 40.0 33.0 40.0 24-25 37.933104592909764 40.0 37.0 40.0 33.0 40.0 26-27 37.83840436462513 40.0 37.0 40.0 33.0 40.0 28-29 37.6755500383513 40.0 37.0 40.0 33.0 40.0 30-31 37.63912991388213 40.0 37.0 40.0 33.0 40.0 32-33 37.50188233899943 40.0 37.0 40.0 33.0 40.0 34-35 37.39072243687663 40.0 37.0 40.0 33.0 40.0 36-37 37.22382019296758 40.0 37.0 40.0 33.0 40.0 38-39 37.16198305510736 40.0 37.0 40.0 33.0 40.0 40-41 37.10786116960389 40.0 37.0 40.0 33.0 40.0 42-43 36.90777085848306 37.0 37.0 40.0 33.0 40.0 44-45 36.770442088237935 37.0 37.0 40.0 33.0 40.0 46-47 36.733297065637366 37.0 37.0 40.0 33.0 40.0 48-49 36.49777933613838 37.0 37.0 40.0 33.0 40.0 50-51 36.37133504188691 37.0 37.0 40.0 33.0 40.0 52-53 36.24330428010903 37.0 37.0 40.0 33.0 40.0 54-55 36.01068998477668 37.0 37.0 40.0 33.0 40.0 56-57 35.80981226746677 37.0 37.0 40.0 27.0 40.0 58-59 35.609046934435554 37.0 33.0 40.0 27.0 40.0 60-61 35.29362528503023 37.0 33.0 40.0 27.0 40.0 62-63 35.04525329436997 37.0 33.0 38.5 27.0 40.0 64-65 34.79126072241083 37.0 33.0 37.0 27.0 40.0 66-67 34.33856206724565 37.0 33.0 37.0 27.0 40.0 68-69 34.061054875563386 37.0 33.0 37.0 27.0 40.0 70-71 33.712331537528925 37.0 33.0 37.0 27.0 38.5 72-73 33.283493705568816 37.0 33.0 37.0 27.0 37.0 74-75 32.72276888557249 37.0 33.0 37.0 22.0 37.0 76-77 30.062387534330597 33.0 30.0 35.0 22.0 37.0 78-79 32.422931276297334 33.0 33.0 37.0 22.0 37.0 80-81 32.828823832759696 37.0 33.0 37.0 27.0 37.0 82-83 32.754671891298486 37.0 33.0 37.0 24.5 37.0 84-85 32.59931377872929 37.0 33.0 37.0 22.0 37.0 86-87 32.4082945588038 35.0 33.0 37.0 22.0 37.0 88-89 32.29698144682713 33.0 33.0 37.0 22.0 37.0 90-91 32.174883546186294 33.0 33.0 37.0 22.0 37.0 92-93 31.967400094543436 33.0 33.0 37.0 22.0 37.0 94-95 31.844171449184206 33.0 33.0 37.0 22.0 37.0 96-97 31.670187042340093 33.0 33.0 37.0 22.0 37.0 98-99 31.55999080610652 33.0 33.0 37.0 15.0 37.0 100-101 29.950738701994137 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17108594613290506 2306 2 0.1626545398296173 2306 3 0.113857203311575 2306 4 0.14705811427489834 2306 5 0.14268211125106944 2306 6 0.14800171307025778 2306 7 0.24756217570336503 2306 8 0.2673900174795065 2306 9 0.20896866202216557 2306 10-11 0.21677346027177435 2306 12-13 0.20992088623420813 2306 14-15 0.4332490254139856 2306 16-17 0.41036339380761433 2306 18-19 0.5027518212526232 2306 20-21 0.5196031095214408 2306 22-23 0.5333832300748043 2306 24-25 0.4835076700705656 2306 26-27 0.5094812874995753 2306 28-29 0.48942025457606064 2306 30-31 0.5541322345919681 2306 32-33 0.5375158379880673 2306 34-35 0.4939215556972272 2306 36-37 0.5900950105247986 2306 38-39 0.6342144463729298 2306 40-41 0.5573283267449298 2306 42-43 0.5793994657826929 2306 44-45 0.6227693641205363 2306 46-47 0.6906589515859807 2306 48-49 0.578662070419071 2306 50-51 0.5654460354250901 2306 52-53 0.6444763074598612 2306 54-55 0.6804485880938245 2306 56-57 0.6629256266446575 2306 58-59 0.6681867642865953 2306 60-61 0.6288769668610641 2306 62-63 0.6631261181297603 2306 64-65 0.6688148280189452 2306 66-67 0.7683065654663181 2306 68-69 0.7659726682013215 2306 70-71 0.6277965051677583 2306 72-73 0.5609423195213878 2306 74-75 0.6776100564864294 2306 76-77 0.09796583133376302 2306 78-79 0.6948139341164676 2306 80-81 0.6640325242639662 2306 82-83 0.65681439787393 2306 84-85 0.7757056671314402 2306 86-87 0.7777467614052327 2306 88-89 0.8631489370038707 2306 90-91 0.778010521037146 2306 92-93 0.7217606579457794 2306 94-95 0.8040858986205919 2306 96-97 0.678732108649907 2306 98-99 0.7550660587524796 2306 100-101 0.6759339046114441 2307 1 0.10710417101578429 2307 2 0.12987231342405892 2307 3 0.1253582708782801 2307 4 0.11830386538895965 2307 5 0.11674119241322245 2307 6 0.17140873069418205 2307 7 0.19763609363787538 2307 8 0.17078503882837737 2307 9 0.19825995925646822 2307 10-11 0.2085754106021298 2307 12-13 0.19928761160732478 2307 14-15 0.4119290034372298 2307 16-17 0.4700611953605289 2307 18-19 0.4874505995137568 2307 20-21 0.477746278859172 2307 22-23 0.43773924202577064 2307 24-25 0.4348476973297508 2307 26-27 0.4424446727604092 2307 28-29 0.5597246969897611 2307 30-31 0.5494589525191245 2307 32-33 0.5580345898830004 2307 34-35 0.586442457663459 2307 36-37 0.5219924500883124 2307 38-39 0.5210795634605532 2307 40-41 0.5512247621867274 2307 42-43 0.5611933373407894 2307 44-45 0.6556378117010908 2307 46-47 0.7278241971558757 2307 48-49 0.7170961584551208 2307 50-51 0.6727664265811413 2307 52-53 0.5688843853456973 2307 54-55 0.6532854092056937 2307 56-57 0.613371719655845 2307 58-59 0.6009509708359317 2307 60-61 0.6162338072904205 2307 62-63 0.6664908163819163 2307 64-65 0.6623852768336107 2307 66-67 0.6350852471597364 2307 68-69 0.6209316825697613 2307 70-71 0.5575402825925337 2307 72-73 0.6618327285914134 2307 74-75 0.6638274929113379 2307 76-77 0.5096193105243749 2307 78-79 0.7962411223245311 2307 80-81 0.7864819615569232 2307 82-83 0.8112286224554168 2307 84-85 0.7832101718903104 2307 86-87 0.7343457058052536 2307 88-89 0.6504781710627228 2307 90-91 0.6986887431195825 2307 92-93 0.7128967641236414 2307 94-95 0.6957934160400612 2307 96-97 0.6610826423347724 2307 98-99 0.7219589127119441 2307 100-101 0.7271820310142338 2201 1 0.1350679749014816 2201 2 0.11448660262366417 2201 3 0.11098928476680925 2201 4 0.11706988323663836 2201 5 0.09554269875150823 2201 6 0.1370034349028586 2201 7 0.11625650017573719 2201 8 0.15141495804630978 2201 9 0.1323476366337175 2201 10-11 0.1549862360821308 2201 12-13 0.18220646544175167 2201 14-15 0.33691206175164723 2201 16-17 0.36845415334232 2201 18-19 0.4187107442189344 2201 20-21 0.35694468339726626 2201 22-23 0.3469447362140343 2201 24-25 0.41462695540091943 2201 26-27 0.38599897803505456 2201 28-29 0.47634746008739626 2201 30-31 0.41484552400334707 2201 32-33 0.4702311280412488 2201 34-35 0.43009037255069416 2201 36-37 0.43354278608288865 2201 38-39 0.4609028788563876 2201 40-41 0.45781005163209443 2201 42-43 0.3442231038860868 2201 44-45 0.48346512827074406 2201 46-47 0.5921261952389685 2201 48-49 0.5433927059877419 2201 50-51 0.5026456916421083 2201 52-53 0.3528755096461822 2201 54-55 0.45360100445387985 2201 56-57 0.4433532048935902 2201 58-59 0.5191909812028896 2201 60-61 0.5326391638884971 2201 62-63 0.473434422290957 2201 64-65 0.4154886754393132 2201 66-67 0.5375696576047204 2201 68-69 0.41822881119438193 2201 70-71 0.30634322414905313 2201 72-73 0.3163650262923312 2201 74-75 0.30636472698797945 2201 76-77 0.08218838915749771 2201 78-79 0.5209655023005695 2201 80-81 0.49599969767975693 2201 82-83 0.4096402732976827 2201 84-85 0.5228981922593761 2201 86-87 0.5926997265996761 2201 88-89 0.4723172705802767 2201 90-91 0.3128467722713566 2201 92-93 0.41476715676388665 2201 94-95 0.39212366884562755 2201 96-97 0.41465513225656636 2201 98-99 0.4658295956746237 2201 100-101 0.33386762924761726 1101 1 0.09495662732492605 1101 2 0.0727863261180346 1101 3 0.07362165543955967 1101 4 0.10687085822583953 1101 5 0.09084850788074306 1101 6 0.1015361890182831 1101 7 0.07644655588425309 1101 8 0.12492112175350911 1101 9 0.12769852602385612 1101 10-11 0.1324681114797599 1101 12-13 0.12601739308399118 1101 14-15 0.23068627068656866 1101 16-17 0.24251069026733063 1101 18-19 0.254493655447412 1101 20-21 0.2743665112195188 1101 22-23 0.2101053907767536 1101 24-25 0.22530764129407999 1101 26-27 0.25117919489314744 1101 28-29 0.2642768061618028 1101 30-31 0.3066394739518401 1101 32-33 0.3025715769765327 1101 34-35 0.3894029159362731 1101 36-37 0.3258966996572852 1101 38-39 0.3082792143507689 1101 40-41 0.31422105578388226 1101 42-43 0.27368510135972457 1101 44-45 0.28354654322191664 1101 46-47 0.3574464213695663 1101 48-49 0.4064788445488361 1101 50-51 0.3296729977245221 1101 52-53 0.35348811699897453 1101 54-55 0.3893580763446778 1101 56-57 0.26913370783435653 1101 58-59 0.37471160011279636 1101 60-61 0.23556697322690923 1101 62-63 0.2591840709654676 1101 64-65 0.3322208587518176 1101 66-67 0.2851486973270454 1101 68-69 0.2847577459765347 1101 70-71 0.16502836049914293 1101 72-73 0.16029198718685223 1101 74-75 0.11161679777481481 1101 76-77 0.04668340942491156 1101 78-79 0.473851921280918 1101 80-81 0.34966419528462467 1101 82-83 0.3196718768980631 1101 84-85 0.4090247519863155 1101 86-87 0.40147820914332755 1101 88-89 0.474328501375588 1101 90-91 0.3425587214256254 1101 92-93 0.3687933884000216 1101 94-95 0.2123379450095797 1101 96-97 0.2734613007589708 1101 98-99 0.19475312111933007 1101 100-101 0.27177800154111864 2304 1 0.1074808214655576 2304 2 0.09692949568426457 2304 3 0.0962955638559464 2304 4 0.06689809258195822 2304 5 0.0828220278216989 2304 6 0.06516469397909219 2304 7 0.09649940646746558 2304 8 0.12800340859301684 2304 9 0.04170760248294414 2304 10-11 0.08519596582822686 2304 12-13 0.14071728616300305 2304 14-15 0.21829949304114393 2304 16-17 0.21497946971260973 2304 18-19 0.24887660061649086 2304 20-21 0.33951042512509133 2304 22-23 0.272552257020962 2304 24-25 0.2554307000060234 2304 26-27 0.2015192820480678 2304 28-29 0.2788035283178516 2304 30-31 0.24614746315268832 2304 32-33 0.26224142264436523 2304 34-35 0.2430308545519324 2304 36-37 0.3423477195142155 2304 38-39 0.35145960959745537 2304 40-41 0.32996859404952517 2304 42-43 0.2469725607353439 2304 44-45 0.34147515759314473 2304 46-47 0.3050547955606575 2304 48-49 0.412233346992835 2304 50-51 0.46267710403808593 2304 52-53 0.3179668554060129 2304 54-55 0.31857728552768094 2304 56-57 0.41274723265458846 2304 58-59 0.4140656492317234 2304 60-61 0.38201521114664416 2304 62-63 0.4200775022329424 2304 64-65 0.44808474671754794 2304 66-67 0.40117274894785737 2304 68-69 0.32233804562913093 2304 70-71 0.30695190514767745 2304 72-73 0.2256105034372169 2304 74-75 0.13169939703769273 2304 76-77 0.015123483261383797 2304 78-79 0.3183257617358848 2304 80-81 0.3843491019994829 2304 82-83 0.3540876451919388 2304 84-85 0.34583799624787304 2304 86-87 0.36101907125758004 2304 88-89 0.42764432779764405 2304 90-91 0.3235113202030746 2304 92-93 0.39564788872800705 2304 94-95 0.33402572825995236 2304 96-97 0.22895656026330258 2304 98-99 0.2721394257731937 2304 100-101 0.19587962875655407 2202 1 0.06365600323523779 2202 2 0.06853925448200471 2202 3 0.037731049013075335 2202 4 0.07853538024102136 2202 5 0.03718843241703951 2202 6 0.04742850026754297 2202 7 0.035613325696587594 2202 8 0.026275716328747478 2202 9 0.061713516008993 2202 10-11 0.023195782777470697 2202 12-13 0.08050076561267616 2202 14-15 0.0992860818326875 2202 16-17 0.041848183679633166 2202 18-19 0.07275944977417481 2202 20-21 0.08157528428424854 2202 22-23 0.09810077218608626 2202 24-25 0.015078762997404738 2202 26-27 -0.005605941387578639 2202 28-29 0.10578793694195099 2202 30-31 0.19167720191349957 2202 32-33 0.1502926609635864 2202 34-35 0.06860012734442478 2202 36-37 0.14920797149400045 2202 38-39 0.13523542064522331 2202 40-41 0.11946694727775053 2202 42-43 0.21598901365553758 2202 44-45 0.2534438685184526 2202 46-47 0.3204199475736331 2202 48-49 0.21004180739070222 2202 50-51 0.28742414186300635 2202 52-53 0.18186043550346653 2202 54-55 0.10868557046536864 2202 56-57 0.16525735730722602 2202 58-59 0.075630511268173 2202 60-61 0.14073455185699402 2202 62-63 0.10543649683486223 2202 64-65 0.18524431135268316 2202 66-67 0.09731780406997359 2202 68-69 0.16809162423039226 2202 70-71 -0.009527198429729822 2202 72-73 0.16550823889653543 2202 74-75 0.06579676634068221 2202 76-77 0.1592462796302918 2202 78-79 0.42869221675239544 2202 80-81 0.23059769863316149 2202 82-83 0.24223487554899492 2202 84-85 0.38360479341584863 2202 86-87 0.3453798089521385 2202 88-89 0.35483531249290223 2202 90-91 0.22722716842549318 2202 92-93 0.19796304239884677 2202 94-95 0.3722127611962094 2202 96-97 0.2633505923700774 2202 98-99 0.16905145809973732 2202 100-101 0.17564010628885285 1102 1 0.03386416327683861 1102 2 0.030156246620151705 1102 3 0.03488766755950934 1102 4 0.028499117206585822 1102 5 0.011147481823030603 1102 6 3.986073544837154E-4 1102 7 0.04083390350308491 1102 8 0.08536697651087621 1102 9 0.0474222963384463 1102 10-11 -0.005252898813232321 1102 12-13 0.05698747740459709 1102 14-15 0.05172464595787574 1102 16-17 0.08788156076708731 1102 18-19 0.13529839145164857 1102 20-21 0.035604760530937085 1102 22-23 0.0781496761055891 1102 24-25 0.03654660746724403 1102 26-27 0.004906759068639133 1102 28-29 0.019626399893397206 1102 30-31 0.015902169183675596 1102 32-33 0.0022679470691855386 1102 34-35 -7.70683562627994E-4 1102 36-37 0.012980757514462482 1102 38-39 0.03737378576458639 1102 40-41 0.10956026229273874 1102 42-43 -0.01872008874006781 1102 44-45 0.09023203478180619 1102 46-47 0.14047726761862123 1102 48-49 0.13514675336028148 1102 50-51 -0.06540005308425378 1102 52-53 -0.023752101916059587 1102 54-55 -0.003367343167489878 1102 56-57 0.0043355646724947405 1102 58-59 0.12074287429713593 1102 60-61 0.052778618448499515 1102 62-63 0.053504203965133 1102 64-65 -0.017015922447534138 1102 66-67 -0.03573798116887872 1102 68-69 -0.05016102060154992 1102 70-71 -0.18290677120135257 1102 72-73 -0.03412837139570968 1102 74-75 -0.06696624084003133 1102 76-77 0.05362094282002161 1102 78-79 0.1666030309361446 1102 80-81 0.052579261609402295 1102 82-83 -0.03785942320758551 1102 84-85 0.06401563693343348 1102 86-87 0.13339446265348442 1102 88-89 0.07392736268746347 1102 90-91 -0.04135709064313886 1102 92-93 -0.06362993830181551 1102 94-95 -0.07960683024751347 1102 96-97 -0.19188852465402206 1102 98-99 -0.3430785949635258 1102 100-101 -0.24575096928333906 2305 1 0.05560484669248211 2305 2 0.01985754116024907 2305 3 0.016192115728053125 2305 4 0.005325572325986627 2305 5 0.014842313114620254 2305 6 3.352188644356602E-5 2305 7 0.01721082214193359 2305 8 0.012997851403071081 2305 9 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#Quality Count 7 1.0 8 11.0 9 24.0 10 51.0 11 51.0 12 78.0 13 324.0 14 979.0 15 2789.0 16 6353.0 17 10740.0 18 13141.0 19 15583.0 20 19182.0 21 23483.0 22 29193.0 23 36393.0 24 45078.0 25 56871.0 26 72228.0 27 97176.0 28 138846.0 29 190631.0 30 184892.0 31 188954.0 32 249433.0 33 354951.0 34 543087.0 35 926271.0 36 1686466.0 37 2256304.0 38 529152.0 39 294.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.30951273027754 27.330371647105835 20.307095839902008 15.053019782714616 2 31.546748864762513 25.712390008607883 28.29979385363478 14.441067272994825 3 34.928630122893445 19.956921530249343 27.154372764197472 17.960075582659744 4 30.88949270832031 19.65720092443244 28.27190421412823 21.181402153119024 5 31.879263982385208 18.246149466577748 31.18435588170909 18.69023066932795 6 29.170283669379256 18.800782913422434 33.24707221373588 18.781861203462423 7 31.79342128737949 22.06263567829707 25.854244232003865 20.28969880231957 8 29.844237733770367 28.394181020730535 22.182247451168834 19.57933379433026 9 29.506173321821432 25.48223013122785 24.70801574682153 20.303580800129183 10-11 24.51517187762485 28.719743821143613 28.928533756304525 17.836550544927015 12-13 22.649970504010284 27.981119962078445 28.56634514084498 20.802564393066294 14-15 22.717296891135707 28.546869974124267 28.4432563572648 20.292576777475222 16-17 23.67581237685587 27.499423753843267 28.94031261842347 19.88445125087739 18-19 21.40017398076054 27.328555373674472 30.770138338145152 20.501132307419837 20-21 22.75754296452277 27.767934929111952 30.435310020432322 19.039212085932952 22-23 21.270847396332837 30.66299178934616 28.742009118000144 19.32415169632086 24-25 21.373640612526877 26.896273087285994 30.68501017709314 21.045076123093992 26-27 21.100389242884173 27.115975887516754 29.125316935386202 22.658317934212874 28-29 22.38500873973622 29.329044150562428 28.52101003938334 19.764937070318005 30-31 20.166418986808257 28.739577895700723 29.99267541323946 21.101327704251563 32-33 20.029905196464142 30.17574186413659 28.692241487196203 21.102111452203058 34-35 22.103398949694586 29.947430034713484 28.781862284362507 19.16730873122942 36-37 21.733287771240093 30.51249671502343 29.03594369921309 18.718271814523384 38-39 20.364336647197455 28.601522078420018 31.427145173087062 19.606996101295472 40-41 20.553970500501347 28.042979546917884 28.112986657686655 23.29006329489411 42-43 20.823957945823647 29.688770886616084 29.821803443518107 19.66546772404216 44-45 20.250562470767896 28.135200307181673 30.015008119386728 21.599229102663703 46-47 22.006619111714894 28.03830224238232 28.622794237148284 21.3322844087545 48-49 20.843086165219713 29.695232387307012 29.479006357850107 19.98267508962317 50-51 22.097987229022387 28.303656670031334 28.892028550752023 20.706327550194256 52-53 20.536636882954777 28.14651940039431 27.766992617845272 23.54985109880564 54-55 22.415414084718382 27.93057805683269 29.495878912258956 20.158128946189972 56-57 20.914716080298433 27.59282558205644 29.476093685455368 22.01636465218976 58-59 20.641219594315178 27.806681354349355 29.8028168830636 21.74928216827187 60-61 21.842624315302352 27.919427682153753 29.583953269436357 20.653994733107535 62-63 20.142886724681773 28.097691464173806 29.62593410657866 22.13348770456576 64-65 22.068291869730224 28.18743797390541 29.564000510534992 20.18026964582937 66-67 21.089056813222307 30.810656047500196 27.846185779965744 20.25410135931175 68-69 20.91864205712538 30.534896266841304 28.056376549587785 20.49008512644553 70-71 20.664359605551955 31.259698880062697 27.925844017517125 20.15009749686822 72-73 20.767644039257295 31.465222609958733 27.717260299953438 20.049873050830534 74-75 21.55876181056818 30.705920499998957 27.405364337659794 20.32995335177307 76-77 21.635431039154305 29.760005075094732 27.683951341617448 20.920612544133522 78-79 21.299429498521732 29.645830895383273 28.26747660984314 20.787262996251858 80-81 20.822856218538536 30.180211801059176 28.116777497143552 20.880154483258735 82-83 20.16221711615159 30.164529368700354 28.304406517830362 21.368846997317696 84-85 20.413382074842946 29.891037473568122 28.450463181738588 21.245117269850336 86-87 20.249187585402282 30.086690337846072 28.886953738819997 20.77716833793165 88-89 20.263276372329834 30.45146331549993 28.779240438763125 20.506019873407112 90-91 20.792009185365387 29.607004707232758 28.54086204036786 21.060124067033996 92-93 20.421143542145455 30.016142967355513 28.001714274362712 21.560999216136324 94-95 20.245163855295186 30.388923720475454 27.672650505997364 21.693261918231993 96-97 20.51027761653506 30.77153901627932 27.359646755130733 21.358536612054888 98-99 20.67588863277261 30.043216446750616 27.657959313874674 21.6229356066021 100-101 20.92384326164049 30.15574360428202 27.274203295431164 21.646209838646325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3492.0 1 2189.5 2 1081.5 3 1682.5 4 1744.5 5 1283.0 6 1247.5 7 1382.5 8 1448.0 9 1479.0 10 1408.5 11 1354.0 12 1424.0 13 1432.5 14 1442.0 15 1513.5 16 1600.5 17 1726.5 18 1980.0 19 2375.5 20 2948.5 21 3734.0 22 4817.5 23 6485.0 24 8701.5 25 11746.5 26 15721.5 27 20866.5 28 27072.0 29 34117.0 30 42459.0 31 52652.5 32 67880.0 33 90787.0 34 122756.0 35 174279.0 36 246087.5 37 332562.0 38 415999.0 39 457560.0 40 483389.5 41 478738.0 42 452849.5 43 463279.0 44 460451.5 45 446113.5 46 456144.5 47 424121.0 48 337840.0 49 266384.0 50 221392.5 51 195009.5 52 175793.5 53 150417.0 54 118220.5 55 86753.0 56 64841.0 57 53465.0 58 45979.5 59 35984.0 60 25888.0 61 19065.0 62 13728.5 63 9548.0 64 6741.0 65 4698.0 66 3407.0 67 2509.0 68 1833.0 69 1231.0 70 762.5 71 506.0 72 344.0 73 243.0 74 164.5 75 100.0 76 75.0 77 63.0 78 42.0 79 30.0 80 22.0 81 19.5 82 17.5 83 11.5 84 8.5 85 5.0 86 1.5 87 2.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14723252085880864 2 0.0 3 0.0 4 0.01586141963612497 5 1.1720260814870667E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.060290323882896E-4 24-25 0.0 26-27 0.0 28-29 3.255628004130741E-5 30-31 0.0010222671932970527 32-33 0.0010027334252722681 34-35 7.16238160908763E-5 36-37 0.0014585213458505719 38-39 0.007318651753285906 40-41 0.01586793089213323 42-43 0.005710371519245319 44-45 0.0021682482507510733 46-47 4.4276540856178073E-4 48-49 0.0061336031597823155 50-51 0.002513344819188932 52-53 0.009949199180623543 54-55 0.0217215500435603 56-57 0.018563590879553483 58-59 0.017905954022719075 60-61 0.023538190469865255 62-63 0.00716238160908763 64-65 0.010287784493053141 66-67 0.027002178666260368 68-69 0.03928891875384978 70-71 0.03732251943935481 72-73 0.027588191707003894 74-75 0.03690579905482608 76-77 0.030029912710101952 78-79 0.012247672551539846 80-81 0.009988266716673112 82-83 0.0045643904617912985 84-85 0.007090757792996753 86-87 0.02267870467677474 88-89 0.020373720049850177 90-91 0.014904265002910531 92-93 0.029854108797878896 94-95 0.031110781207473362 96-97 0.03284277530567091 98-99 0.02245732197249385 100-101 0.022717772212824307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7679010.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.63893839235676 #Duplication Level Percentage of deduplicated Percentage of total 1 88.24769155456094 36.745401919080656 2 6.0386708265192794 5.028876850343168 3 1.6662554103023937 2.0814331912653756 4 0.9088302076887063 1.5137090010825136 5 0.5417580947775729 1.1279115966001967 6 0.36999490557152853 0.9243717047147241 7 0.2582919788917787 0.752850265742028 8 0.21321963292883664 0.7102591327651802 9 0.17240277579778512 0.6460801704103744 >10 1.1999515086696526 10.28977345824008 >50 0.18094359589411574 5.269494967112571 >100 0.16558252737695475 14.48932089089658 >500 0.021381905924209233 6.333572917116016 >1k 0.014651377528165173 10.190885480366703 >5k 2.4921175570513475E-4 0.6934628937727828 >10k+ 1.2448581219952985E-4 3.20259556049129 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 207557 2.702913526613457 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17277 0.22498994010946724 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12170 0.15848397124108446 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10136 0.13199618179947675 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9588 0.12485984521442217 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9537 0.12419569710157949 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.002122669458693243 0.0 0.0 2 0.002370097187007179 0.0 0.0 3 0.0026045024033045927 0.0 0.0 4 0.00281286259556896 0.0 0.0 5 0.003490033220428154 0.0 0.0 6 0.004154181333270825 0.0 0.0 7 0.004740194374014358 0.0 0.0 8 0.005508522582989213 0.0 0.0 9 0.006224760743897976 0.0 0.0 10-11 0.010561257245400123 0.0 0.0 12-13 0.014311740706158737 0.0 0.0 14-15 0.019833285801164474 0.0 0.0 16-17 0.028083047163631768 0.0 0.0 18-19 0.0383057190966023 0.0 0.0 20-21 0.04434165341626069 0.0 0.0 22-23 0.04995435609538208 0.0 0.0 24-25 0.05617911683928006 0.0 6.511256008261482E-6 26-27 0.0634066110084503 0.0 1.3022512016522964E-5 28-29 0.07129825329046321 0.0 1.3022512016522964E-5 30-31 0.12017825214448216 0.0 1.9533768024784447E-5 32-33 0.13155992764692323 0.0 2.6045024033045928E-5 34-35 0.14026547692996882 0.0 3.255628004130741E-5 36-37 0.14999980466231977 0.0 3.9067536049568887E-5 38-39 0.16022247659529026 0.0 3.9067536049568887E-5 40-41 0.17116789794517784 0.0 4.557879205783037E-5 42-43 0.1827318886158502 0.0 5.2090048066091855E-5 44-45 0.196073452176778 0.0 5.2090048066091855E-5 46-47 0.2102875240428128 0.0 6.511256008261482E-5 48-49 0.22650055150338388 0.0 7.162381609087629E-5 50-51 0.24415256654178077 0.0 7.813507209913777E-5 52-53 0.26211712186857417 0.0 9.115758411566074E-5 54-55 0.28133834960496207 0.0 9.115758411566074E-5 56-57 0.30285805071226635 0.0 9.766884012392223E-5 58-59 0.3263636849020902 0.0 1.0418009613218371E-4 60-61 0.35106739019743427 0.0 1.0418009613218371E-4 62-63 0.3779458549995377 0.0 1.0418009613218371E-4 64-65 0.40707070312449134 0.0 1.0418009613218371E-4 66-67 0.4392297965492947 0.0 1.0418009613218371E-4 68-69 0.47444266904197285 0.0 1.0418009613218371E-4 70-71 0.5131781310351204 0.0 1.0418009613218371E-4 72-73 0.5562826458098113 0.0 1.0418009613218371E-4 74-75 0.6035478531737815 0.0 1.0418009613218371E-4 76-77 0.6564452969848977 0.0 1.1720260814870667E-4 78-79 0.716537678685143 0.0 1.1720260814870667E-4 80-81 0.7833106090498645 0.0 1.1720260814870667E-4 82-83 0.8583593458010863 0.0 1.1720260814870667E-4 84-85 0.9414690174905359 0.0 1.1720260814870667E-4 86-87 1.0308294949479164 0.0 1.2371386415696816E-4 88-89 1.129481534729086 0.0 1.3022512016522964E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 35170 0.0 65.01722 4 TCGGAAG 35325 0.0 64.70504 3 GATCGGA 35965 0.0 63.873394 1 GAAGAGC 36125 0.0 63.25894 6 ATCGGAA 36620 0.0 62.494694 2 GGAAGAG 41135 0.0 55.843006 5 CGAGTCG 75 1.3482168E-7 44.399662 1 GCCGTCT 26275 0.0 43.507133 50-51 ATGCCGT 26305 0.0 43.26536 48-49 TATGCCG 26230 0.0 43.189877 48-49 TGCCGTC 26370 0.0 43.04416 50-51 TCGTATG 26595 0.0 43.00147 44-45 CGTATGC 26520 0.0 42.739025 46-47 TCTCGTA 26365 0.0 42.62918 42-43 CTCGTAT 26340 0.0 42.615406 44-45 CATCTCG 26180 0.0 42.5899 40-41 CGTCTTC 26980 0.0 42.353077 52-53 ATCTCGT 26370 0.0 42.07178 42-43 GTATGCC 27365 0.0 41.783764 46-47 TGTGTTT 88100 0.0 41.34525 1 >>END_MODULE