FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7679010
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2075572.702913526613457TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172770.22498994010946724No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA121700.15848397124108446No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC101360.13199618179947675No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG95880.12485984521442217No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC95370.12419569710157949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA351700.065.017224
TCGGAAG353250.064.705043
GATCGGA359650.063.8733941
GAAGAGC361250.063.258946
ATCGGAA366200.062.4946942
GGAAGAG411350.055.8430065
CGAGTCG751.3482168E-744.3996621
GCCGTCT262750.043.50713350-51
ATGCCGT263050.043.2653648-49
TATGCCG262300.043.18987748-49
TGCCGTC263700.043.0441650-51
TCGTATG265950.043.0014744-45
CGTATGC265200.042.73902546-47
TCTCGTA263650.042.6291842-43
CTCGTAT263400.042.61540644-45
CATCTCG261800.042.589940-41
CGTCTTC269800.042.35307752-53
ATCTCGT263700.042.0717842-43
GTATGCC273650.041.78376446-47
TGTGTTT881000.041.345251
Produced by FastQC (version 0.11.2)