##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6028003 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.685424509576386 33.0 33.0 33.0 33.0 33.0 2 32.71813517677413 33.0 33.0 33.0 33.0 33.0 3 32.75059136500098 33.0 33.0 33.0 33.0 33.0 4 36.680089907055454 37.0 37.0 37.0 37.0 37.0 5 36.685946241234454 37.0 37.0 37.0 37.0 37.0 6 36.553570560598594 37.0 37.0 37.0 37.0 37.0 7 36.48988960357186 37.0 37.0 37.0 37.0 37.0 8 36.41677998501328 37.0 37.0 37.0 37.0 37.0 9 36.45758172316769 37.0 37.0 37.0 37.0 37.0 10-11 36.40116502928084 37.0 37.0 37.0 37.0 37.0 12-13 36.376587652660426 37.0 37.0 37.0 37.0 37.0 14-15 38.23674407594024 40.0 37.0 40.0 37.0 40.0 16-17 38.22784602131087 40.0 37.0 40.0 37.0 40.0 18-19 38.193812279124614 40.0 37.0 40.0 37.0 40.0 20-21 38.1656517921441 40.0 37.0 40.0 37.0 40.0 22-23 38.03401765062161 40.0 37.0 40.0 35.0 40.0 24-25 38.04746538779095 40.0 37.0 40.0 35.0 40.0 26-27 37.98135394093201 40.0 37.0 40.0 35.0 40.0 28-29 37.82831047031662 40.0 37.0 40.0 33.0 40.0 30-31 37.81290785688063 40.0 37.0 40.0 33.0 40.0 32-33 37.68746647936307 40.0 37.0 40.0 33.0 40.0 34-35 37.56875568907314 40.0 37.0 40.0 33.0 40.0 36-37 37.34822909013151 40.0 37.0 40.0 33.0 40.0 38-39 37.394186847617696 40.0 37.0 40.0 33.0 40.0 40-41 37.241794338854845 40.0 37.0 40.0 33.0 40.0 42-43 37.09754424475236 40.0 37.0 40.0 33.0 40.0 44-45 37.03568868495918 38.5 37.0 40.0 33.0 40.0 46-47 36.9927286034861 38.5 37.0 40.0 33.0 40.0 48-49 36.76495142752915 37.0 37.0 40.0 33.0 40.0 50-51 36.67023722450038 37.0 37.0 40.0 33.0 40.0 52-53 36.56862363870755 37.0 37.0 40.0 33.0 40.0 54-55 36.33583667758626 37.0 37.0 40.0 33.0 40.0 56-57 36.171893162627825 37.0 37.0 40.0 33.0 40.0 58-59 35.94632393182286 37.0 37.0 40.0 33.0 40.0 60-61 35.67709803727702 37.0 37.0 40.0 27.0 40.0 62-63 35.44084981377746 37.0 33.0 40.0 27.0 40.0 64-65 35.195244428378686 37.0 33.0 38.5 27.0 40.0 66-67 34.73729915197454 37.0 33.0 37.0 27.0 40.0 68-69 34.48369957679185 37.0 33.0 37.0 27.0 40.0 70-71 34.11851677910579 37.0 33.0 37.0 27.0 40.0 72-73 33.64007317182822 37.0 33.0 37.0 27.0 38.5 74-75 33.0327189618187 37.0 33.0 37.0 22.0 37.0 76-77 30.413412783636637 33.0 30.0 35.0 22.0 37.0 78-79 32.67260426048229 35.0 33.0 37.0 24.5 37.0 80-81 32.99616075174481 37.0 33.0 37.0 27.0 37.0 82-83 32.90905636576491 37.0 33.0 37.0 27.0 37.0 84-85 32.74740108788931 37.0 33.0 37.0 22.0 37.0 86-87 32.55196613206729 37.0 33.0 37.0 22.0 37.0 88-89 32.42469147742627 37.0 33.0 37.0 22.0 37.0 90-91 32.29372803895419 33.0 33.0 37.0 22.0 37.0 92-93 32.09548095778983 33.0 33.0 37.0 22.0 37.0 94-95 31.96746542760513 33.0 33.0 37.0 22.0 37.0 96-97 31.79804207131284 33.0 33.0 37.0 22.0 37.0 98-99 31.7024342887686 33.0 33.0 37.0 18.5 37.0 100-101 30.13828642752832 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12690467257967697 2306 2 0.12625586721308224 2306 3 0.09548435320098747 2306 4 0.12131556833709567 2306 5 0.11103426394146254 2306 6 0.1302915706621448 2306 7 0.19035702008679323 2306 8 0.22634039995298139 2306 9 0.14742507119538573 2306 10-11 0.1592430429140137 2306 12-13 0.2135677540530665 2306 14-15 0.4385422218852497 2306 16-17 0.44796740953786696 2306 18-19 0.47864703017974364 2306 20-21 0.4506403232499494 2306 22-23 0.41925221067842955 2306 24-25 0.3888040625754883 2306 26-27 0.4700756298165132 2306 28-29 0.45202046382649286 2306 30-31 0.46772248302094965 2306 32-33 0.5013943455850551 2306 34-35 0.47922354707811365 2306 36-37 0.5338300508730285 2306 38-39 0.6211601523301837 2306 40-41 0.5243851426626449 2306 42-43 0.5536385847537417 2306 44-45 0.6380012477136532 2306 46-47 0.6362530688831427 2306 48-49 0.5530492821104005 2306 50-51 0.4342487928739729 2306 52-53 0.5640147890209946 2306 54-55 0.6475335152187895 2306 56-57 0.6381477810659888 2306 58-59 0.6418973398694519 2306 60-61 0.6004254016469375 2306 62-63 0.5391310968071323 2306 64-65 0.4769408765998264 2306 66-67 0.5721325194704363 2306 68-69 0.518370898801713 2306 70-71 0.47360251339676296 2306 72-73 0.37246411688851566 2306 74-75 0.39341329724648944 2306 76-77 -0.1432976921432072 2306 78-79 0.4282149197134899 2306 80-81 0.5119406882030191 2306 82-83 0.5766941000088579 2306 84-85 0.5389647294573265 2306 86-87 0.5893639962803547 2306 88-89 0.5913160997147884 2306 90-91 0.49480359204199686 2306 92-93 0.544173439891587 2306 94-95 0.5215222447111145 2306 96-97 0.4448637254201451 2306 98-99 0.5476338896319746 2306 100-101 0.3953131089672688 2307 1 0.12386291932065774 2307 2 0.13954936113771765 2307 3 0.13051507213315006 2307 4 0.14896044651127482 2307 5 0.08462839285515145 2307 6 0.11683784740789349 2307 7 0.12037115373201601 2307 8 0.18799622491518164 2307 9 0.12459627002878904 2307 10-11 0.2282268388088866 2307 12-13 0.21226059963620258 2307 14-15 0.36873353858583613 2307 16-17 0.3877099068216694 2307 18-19 0.43096711430568035 2307 20-21 0.4385847837308674 2307 22-23 0.4358163331596856 2307 24-25 0.42550234110747454 2307 26-27 0.41002902369200456 2307 28-29 0.47893248119242315 2307 30-31 0.41248430810615133 2307 32-33 0.29128823520743197 2307 34-35 0.47310837698403674 2307 36-37 0.3414198072768002 2307 38-39 0.3492659391464983 2307 40-41 0.3858910435010756 2307 42-43 0.3617209964821768 2307 44-45 0.5628844969339681 2307 46-47 0.5766001105411149 2307 48-49 0.39081522983197914 2307 50-51 0.47911555376494164 2307 52-53 0.40819766653364553 2307 54-55 0.3757623827597669 2307 56-57 0.40900371705847505 2307 58-59 0.42810113473380085 2307 60-61 0.44819371513350603 2307 62-63 0.4436810114013525 2307 64-65 0.4300981478921244 2307 66-67 0.403340588779038 2307 68-69 0.3684202917641386 2307 70-71 0.21951688710895922 2307 72-73 0.26961746998642155 2307 74-75 0.21118246395978701 2307 76-77 0.10826687080248476 2307 78-79 0.4101040744816089 2307 80-81 0.3937718900393392 2307 82-83 0.3703582544584094 2307 84-85 0.4170003743886994 2307 86-87 0.40103624862714327 2307 88-89 0.35718761263535725 2307 90-91 0.22473930085455152 2307 92-93 0.301673302765856 2307 94-95 0.22265301943543392 2307 96-97 0.25056239344458575 2307 98-99 0.2570572524200969 2307 100-101 0.3692795272932514 2201 1 0.16089553058383643 2201 2 0.15066475299204285 2201 3 0.09351378748912964 2201 4 0.12452694673422826 2201 5 0.09369972280908456 2201 6 0.14886729442990543 2201 7 0.16277956442399244 2201 8 0.18440755858283353 2201 9 0.13703243256846775 2201 10-11 0.17247063569618604 2201 12-13 0.24129229131153807 2201 14-15 0.3579722133182699 2201 16-17 0.3516566313583738 2201 18-19 0.3802753391853315 2201 20-21 0.3792718724900439 2201 22-23 0.33740603276519465 2201 24-25 0.40535806181550527 2201 26-27 0.38135038806732524 2201 28-29 0.38476340081817995 2201 30-31 0.3455853023473523 2201 32-33 0.40188016936090065 2201 34-35 0.42279428973183286 2201 36-37 0.4596804472745717 2201 38-39 0.4052458496887894 2201 40-41 0.41129958429957725 2201 42-43 0.4459373067874992 2201 44-45 0.44904912084401616 2201 46-47 0.5289134171541789 2201 48-49 0.43043367666324883 2201 50-51 0.4828230844062915 2201 52-53 0.39770691230739885 2201 54-55 0.4220847260919314 2201 56-57 0.48255268195371315 2201 58-59 0.4509717141120362 2201 60-61 0.48818568137149754 2201 62-63 0.34984945008518054 2201 64-65 0.38530727202259385 2201 66-67 0.37530402253018735 2201 68-69 0.45688540111046194 2201 70-71 0.3691268833320791 2201 72-73 0.36430457642997993 2201 74-75 0.28861277170592814 2201 76-77 0.1752496247407329 2201 78-79 0.6525540434707864 2201 80-81 0.5378740855553445 2201 82-83 0.5915334068914646 2201 84-85 0.6370705055941741 2201 86-87 0.6578326723398717 2201 88-89 0.5959050402146531 2201 90-91 0.5375991306550034 2201 92-93 0.5323351154907883 2201 94-95 0.5951496372974177 2201 96-97 0.6218075886612162 2201 98-99 0.6878460137428348 2201 100-101 0.6113756823296974 1101 1 0.0997259076008632 1101 2 0.0980804075815982 1101 3 0.07811008639873762 1101 4 0.07142131186988365 1101 5 0.0734767529665632 1101 6 0.05609982725671614 1101 7 0.10082070162474821 1101 8 0.18169924856125874 1101 9 0.15715797852600133 1101 10-11 0.08980443480230349 1101 12-13 0.12276272614480632 1101 14-15 0.2736782891100731 1101 16-17 0.29106045451760565 1101 18-19 0.24124199959442905 1101 20-21 0.2850307708002191 1101 22-23 0.2023472593906277 1101 24-25 0.25524403841713195 1101 26-27 0.2536515337574201 1101 28-29 0.2652827390200869 1101 30-31 0.30185009279122 1101 32-33 0.3082139009592524 1101 34-35 0.30423245030230106 1101 36-37 0.2598851437809131 1101 38-39 0.392210700334779 1101 40-41 0.27837516618419045 1101 42-43 0.27996219403564737 1101 44-45 0.40557152741279623 1101 46-47 0.362232362701306 1101 48-49 0.39630295017791894 1101 50-51 0.3791348944745607 1101 52-53 0.39973995967288545 1101 54-55 0.44483889053531556 1101 56-57 0.3673699069774585 1101 58-59 0.3961189689039415 1101 60-61 0.2605095300172309 1101 62-63 0.3851638282230212 1101 64-65 0.40524102193127476 1101 66-67 0.38453392995206315 1101 68-69 0.31086052218518745 1101 70-71 0.19301182604757372 1101 72-73 0.20677628096419198 1101 74-75 0.20951770874336972 1101 76-77 0.15838461161370887 1101 78-79 0.31086672374195956 1101 80-81 0.4340187676538534 1101 82-83 0.3753407896521992 1101 84-85 0.35167014752556014 1101 86-87 0.37509771524430136 1101 88-89 0.41460048491978085 1101 90-91 0.411884103147905 1101 92-93 0.3926432514717817 1101 94-95 0.2666118833456679 1101 96-97 0.38949388467631607 1101 98-99 0.34827746700018025 1101 100-101 0.402027590071544 2304 1 0.12581725707265434 2304 2 0.11256472476159729 2304 3 0.11974680281552708 2304 4 0.11593517876837467 2304 5 0.1118848906891472 2304 6 0.08712411953029431 2304 7 0.10827304958993267 2304 8 0.19138830000436258 2304 9 0.11036650376942703 2304 10-11 0.10267083365953056 2304 12-13 0.17957171944544825 2304 14-15 0.21625093627489633 2304 16-17 0.28731660607940057 2304 18-19 0.267266616235716 2304 20-21 0.3095852490726898 2304 22-23 0.26795622749452974 2304 24-25 0.21817405187390904 2304 26-27 0.2268838955467558 2304 28-29 0.24302148216154507 2304 30-31 0.25666022836795577 2304 32-33 0.24635521040478636 2304 34-35 0.26024149191759705 2304 36-37 0.2785325680952653 2304 38-39 0.28159938728536105 2304 40-41 0.327884116492676 2304 42-43 0.30608692112323865 2304 44-45 0.29245250256605715 2304 46-47 0.3579001497806118 2304 48-49 0.3339078656568333 2304 50-51 0.26381998092572445 2304 52-53 0.3472252318559086 2304 54-55 0.3788978711569442 2304 56-57 0.37869965864620525 2304 58-59 0.3958116260255835 2304 60-61 0.2098798534548436 2304 62-63 0.27652166009911383 2304 64-65 0.3650522220075416 2304 66-67 0.29075002031051866 2304 68-69 0.24830932450672805 2304 70-71 0.12317120349097621 2304 72-73 0.10833944207619339 2304 74-75 0.06163721870800032 2304 76-77 -0.12489538025738511 2304 78-79 0.09740770867642823 2304 80-81 0.1397228409057547 2304 82-83 0.13988774026425688 2304 84-85 0.28507031225417734 2304 86-87 0.21863427211160769 2304 88-89 0.29525489944771977 2304 90-91 0.18218730361771662 2304 92-93 0.24560578144667033 2304 94-95 0.26328881687735617 2304 96-97 0.029324545022650028 2304 98-99 0.08153070749624902 2304 100-101 0.1259759577224422 2202 1 0.08382948249237643 2202 2 0.04258820431031296 2202 3 0.02439960651445716 2202 4 0.05333480871463081 2202 5 0.0236001018244707 2202 6 0.06996353948510148 2202 7 0.08723786237565179 2202 8 0.07659839179535055 2202 9 0.09060491149480043 2202 10-11 0.07394258234407403 2202 12-13 0.15053426053465557 2202 14-15 0.10884177403321615 2202 16-17 0.09581399523385414 2202 18-19 0.19288940624826978 2202 20-21 0.13473694301175243 2202 22-23 0.10072894144941102 2202 24-25 0.02799729301489151 2202 26-27 0.05509166346446648 2202 28-29 0.07873674547364118 2202 30-31 0.14676791320417948 2202 32-33 0.06828749699174352 2202 34-35 0.07206836135123496 2202 36-37 0.11578570171195679 2202 38-39 0.1583710697455345 2202 40-41 0.07022643225848668 2202 42-43 0.20547037225567522 2202 44-45 0.1877571547275707 2202 46-47 0.294243139830904 2202 48-49 0.2674942362805055 2202 50-51 0.18664057710557813 2202 52-53 0.10445773557852789 2202 54-55 0.0462932466892525 2202 56-57 0.2020561303724122 2202 58-59 0.15526204404182664 2202 60-61 0.19331595496675646 2202 62-63 0.10358699746210931 2202 64-65 0.17832013369233124 2202 66-67 0.16667862642804465 2202 68-69 0.17068932795842073 2202 70-71 0.021053027109395828 2202 72-73 0.08714108203236748 2202 74-75 -0.023239122335446893 2202 76-77 0.02232271464815483 2202 78-79 0.22922440363895902 2202 80-81 0.16917454327337822 2202 82-83 0.13439683708428163 2202 84-85 0.2553589300914112 2202 86-87 0.23498581112578876 2202 88-89 0.1612727679863326 2202 90-91 0.08460055972942371 2202 92-93 0.14615861444486455 2202 94-95 0.12293343283621994 2202 96-97 0.07204566417433611 2202 98-99 0.049269185459593245 2202 100-101 0.16225735911034533 1102 1 0.09992601792230005 1102 2 0.05606819176284006 1102 3 0.033373583862271516 1102 4 0.01571502375691125 1102 5 0.009860221559371496 1102 6 -0.0024983008550165664 1102 7 -0.002410074299667997 1102 8 0.052482888328107435 1102 9 0.04026032325872109 1102 10-11 0.024454892880612533 1102 12-13 0.05280274969911858 1102 14-15 0.016783087913523786 1102 16-17 0.0086618037648023 1102 18-19 0.04203601142343416 1102 20-21 -0.001103036698715698 1102 22-23 -0.019439047818501365 1102 24-25 0.025545997889054206 1102 26-27 -0.01846721286953823 1102 28-29 0.006471626773240757 1102 30-31 0.0033546676141469334 1102 32-33 0.01846693248907627 1102 34-35 -0.008578834489405551 1102 36-37 0.0725903015611209 1102 38-39 0.00770154875188922 1102 40-41 -0.011932833848000257 1102 42-43 -0.027842793911538877 1102 44-45 0.08930670054920142 1102 46-47 0.13836281466455347 1102 48-49 0.008318464911432955 1102 50-51 -0.034105062982249024 1102 52-53 -0.101148914580655 1102 54-55 -0.03454196097805351 1102 56-57 -0.044560691246609 1102 58-59 0.09781765669875853 1102 60-61 0.015535755942529761 1102 62-63 -0.14711793008959972 1102 64-65 -0.1311487345659046 1102 66-67 -0.05689479963199062 1102 68-69 -0.08316411358571685 1102 70-71 -0.16232457832606428 1102 72-73 -0.1640309384440357 1102 74-75 -0.29444791757808986 1102 76-77 -0.0029735191085435986 1102 78-79 0.0901815820599765 1102 80-81 -0.0010336245957560664 1102 82-83 -0.04136101676438386 1102 84-85 -0.01825527524660231 1102 86-87 0.03687130211229572 1102 88-89 0.030285856065567884 1102 90-91 -0.1117669476622396 1102 92-93 -0.020560088627256334 1102 94-95 -5.486569849750822E-4 1102 96-97 -0.16506024181307666 1102 98-99 -0.30393552518106404 1102 100-101 -0.2811221150972578 2305 1 0.059644396528554466 2305 2 0.04508086749451934 2305 3 0.053270791849776344 2305 4 0.03477423123210599 2305 5 0.028647447463626463 2305 6 0.032413812178077706 2305 7 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-0.4800696211518485 1213 80-81 -0.5633840809642763 1213 82-83 -0.40626274879753055 1213 84-85 -0.5306526870273487 1213 86-87 -0.5993457428507227 1213 88-89 -0.5753037875951179 1213 90-91 -0.40547968721129735 1213 92-93 -0.2518389055948802 1213 94-95 -0.4459973041604748 1213 96-97 -0.36434553196677655 1213 98-99 -0.3521259900503608 1213 100-101 -0.13622023550409423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 23.0 10 31.0 11 43.0 12 86.0 13 167.0 14 471.0 15 1138.0 16 2243.0 17 3724.0 18 5773.0 19 8289.0 20 11302.0 21 15193.0 22 19654.0 23 25023.0 24 32708.0 25 41536.0 26 55321.0 27 77109.0 28 119562.0 29 165655.0 30 142280.0 31 137341.0 32 179813.0 33 253945.0 34 386515.0 35 653099.0 36 1232147.0 37 1896598.0 38 560677.0 39 531.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.274538709951294 25.20394754659006 22.058151862480845 15.463361880977805 2 28.873094455991478 25.865481486986653 31.566092452176946 13.69533160484492 3 35.83481959116477 19.414373217797003 28.263074852484316 16.48773233855391 4 28.007818695981413 19.82486744851414 31.97276563888522 20.194548216619225 5 34.77353509407269 18.400487193260513 30.20949907415364 16.61647863851315 6 29.04962057915366 19.43121129833545 34.722278671725945 16.796889450784942 7 31.03603299467502 23.25237727983878 27.475998270073852 18.235591455412347 8 27.726446055186106 29.338638351706198 25.32513669950065 17.609778893607054 9 32.12131778965605 23.52931808428098 25.791261218682205 18.558102907380768 10-11 24.50045230568067 30.078717611786193 29.17873464893763 16.242095433595505 12-13 22.659535836329212 28.53417624377427 29.0843916301966 19.721896289699924 14-15 21.86667790311319 28.839667133543234 29.648077481049697 19.645577482293888 16-17 23.19880232309108 27.270830820754405 30.29586249376452 19.23450436239 18-19 20.689613127266192 27.40377700541954 32.390644132061645 19.515965735252617 20-21 22.793742803379494 27.703627552939174 31.919227644710862 17.583401998970473 22-23 20.82663575529976 31.669151603250672 29.650509394719215 17.85370324673036 24-25 20.709561690662728 27.350683136687227 31.791200170271978 20.148555002378068 26-27 20.65265229629116 27.633330640346397 29.45656297782201 22.257454085540434 28-29 22.34060307212904 29.66282194332384 29.409089446221152 18.587485538325964 30-31 19.96249133203051 27.989503876946355 31.413965633367287 20.63403915765585 32-33 19.957915964312182 30.089695665467087 29.667237840329836 20.285150529890895 34-35 22.310665103294696 29.89336754702558 29.756058232288147 18.039909117391584 36-37 20.221594309468085 29.70903252911562 31.858277520018348 18.211095641397947 38-39 20.82431672967609 27.4537819134265 31.183132415186137 20.53876894171127 40-41 22.614249277108083 27.698804091416847 30.755437088804022 18.93150954267105 42-43 20.361832066514364 29.860813465589835 31.160105119250424 18.617249348645377 44-45 20.137378315427647 27.747151569042515 31.50124919540535 20.614220920124488 46-47 22.312617421273313 27.645403307080734 29.345443536179232 20.69653573546672 48-49 20.36696413625347 30.04805877743766 30.78824325160565 18.796733834703222 50-51 22.745317023021336 27.889536937176366 30.288089786014666 19.07705625378763 52-53 20.561238181550465 27.728605469616813 28.358613080708682 23.351543268124043 54-55 22.664522417752377 27.92854620139319 30.20049153233792 19.206439848516514 56-57 20.47444589352262 28.172900468240297 30.321015062669865 21.031638575567214 58-59 20.475557042968745 28.15821117067585 30.61131741871277 20.754914367642634 60-61 22.265089836838218 28.39195531716287 30.51176171764195 18.83119312835696 62-63 20.13391168839701 28.26415754515368 30.447290024503303 21.154640741946007 64-65 22.144926285890627 28.022261893306165 30.38240749650758 19.450404324295626 66-67 20.329326281062936 31.431259156168505 28.565561464528972 19.673853098239587 68-69 20.164411474449864 31.831547111343934 28.51890517040298 19.485136243803225 70-71 19.897084830832796 32.48138257553285 28.271766477790816 19.349766115843543 72-73 19.924545684824118 32.35103555741666 28.052573923731423 19.671844834027805 74-75 20.144282586160102 31.627461137789698 28.336855304767735 19.89140097128247 76-77 20.083587372002636 31.31351122304549 28.46768073045649 20.13522067449538 78-79 19.87593628278882 31.148950707087366 28.699631364381506 20.275481645742307 80-81 19.77351502324685 31.267116176850106 28.43065775477237 20.52871104513067 82-83 19.50094828684582 30.973220021129077 28.587248362230977 20.938583329794124 84-85 19.67267142614448 30.709639667977978 28.823376388955253 20.794312516922293 86-87 20.002060866411803 30.544587267944078 29.09395800262885 20.35939386301527 88-89 20.255395864004026 30.61071751685929 28.726044711698606 20.407841907438083 90-91 20.294102470379784 30.50060651572602 28.595553147153772 20.609737866740424 92-93 19.874847373834292 30.928358713224107 28.702256925718228 20.494536987223377 94-95 19.56493347983021 31.133839036306764 28.715669772672626 20.5855577111904 96-97 19.790216346911784 31.24110309211629 28.560970267027546 20.40771029394438 98-99 20.29270740368717 30.7364593808597 28.154663032554765 20.816170182898365 100-101 20.47828481062175 30.630732058242355 27.624755115943522 21.26622801519237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 160.0 1 95.0 2 46.0 3 88.0 4 100.0 5 79.5 6 66.5 7 58.5 8 58.0 9 65.5 10 69.0 11 75.5 12 88.5 13 100.0 14 117.5 15 153.5 16 203.5 17 276.0 18 372.5 19 526.5 20 848.0 21 1343.0 22 2200.0 23 3517.5 24 5373.5 25 8335.0 26 13280.5 27 19889.0 28 27657.0 29 36937.0 30 47575.0 31 59454.0 32 77377.0 33 106449.5 34 144293.0 35 192443.0 36 261756.5 37 333811.0 38 394098.5 39 426011.5 40 433155.0 41 422754.0 42 393022.0 43 365197.0 44 330978.0 45 297759.5 46 277034.5 47 248567.5 48 211937.0 49 183484.5 50 154133.0 51 125695.5 52 102100.0 53 79763.5 54 63610.5 55 50588.0 56 36981.0 57 25330.5 58 17634.5 59 12824.5 60 9296.5 61 6514.5 62 4440.5 63 2997.5 64 2041.0 65 1437.5 66 1002.0 67 687.0 68 487.5 69 339.5 70 222.5 71 163.5 72 123.5 73 86.5 74 59.5 75 40.5 76 35.0 77 28.0 78 26.0 79 21.5 80 13.0 81 8.0 82 6.0 83 3.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1522228837643246 2 0.0 3 0.0 4 0.01511279937982778 5 8.29462095489999E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.571332496018997E-4 24-25 0.0 26-27 0.0 28-29 1.658924190979998E-5 30-31 0.0010451222403173985 32-33 0.0010617114822271986 34-35 1.1612469336859986E-4 36-37 0.0015842726023858977 38-39 0.00772229210901189 40-41 0.016514590321205877 42-43 0.006179492611400491 44-45 0.0020404767549053976 46-47 4.644987734743994E-4 48-49 0.006370268893363191 50-51 0.0026542787055679968 52-53 0.010210678395481888 54-55 0.02222128953817707 56-57 0.019625073179293375 58-59 0.018812200325713173 60-61 0.02386362448724727 62-63 0.007440274996545291 64-65 0.010998667386197385 66-67 0.027695739368411063 68-69 0.03963999354346705 70-71 0.03754974906283225 72-73 0.028326130560983463 74-75 0.03746680285328325 76-77 0.03074815987981426 78-79 0.012898135584869483 80-81 0.010409749298399487 82-83 0.004827469395751794 84-85 0.00748174810131979 86-87 0.02341571495568267 88-89 0.02124252426549887 90-91 0.01582613678194918 92-93 0.030540794355941762 94-95 0.032116772337372756 96-97 0.03372592880262336 98-99 0.023299590262314072 100-101 0.02366455358432967 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6028003.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.202287502373046 #Duplication Level Percentage of deduplicated Percentage of total 1 91.36525399244873 49.52205764626043 2 4.206309061283515 4.5598314612705195 3 1.2743099074416593 2.072115359108256 4 0.67420809232383 1.4617448342625083 5 0.41842071447764984 1.133967993153296 6 0.2896202232286443 0.9418847163564262 7 0.18856597632471284 0.7154495083342428 8 0.16511635450694162 0.71597472946632 9 0.13755223696138807 0.671008130493813 >10 1.0400372362251589 11.51170759519985 >50 0.12786718086021506 4.8231933174363295 >100 0.09520181452531884 9.986896600434648 >500 0.014329664729752995 5.408689817470307 >1k 0.0030849337179925536 2.3255296508199743 >5k 0.0 0.0 >10k+ 1.2261094459001273E-4 4.149948639933145 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 217614 3.6100512889592125 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11904 0.19747833569425896 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10144 0.16828126993301099 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10117 0.1678333604014464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.96748160211599E-4 0.0 0.0 2 8.12872853580199E-4 0.0 0.0 3 9.124083050389988E-4 0.0 0.0 4 9.787652726781987E-4 0.0 0.0 5 0.0010451222403173987 0.0 0.0 6 0.0011778361755957986 0.0 0.0 7 0.0013437285946937983 0.0 0.0 8 0.001575977981430998 0.0 1.658924190979998E-5 9 0.0017418704005289977 0.0 1.658924190979998E-5 10-11 0.0028699388503953963 0.0 1.658924190979998E-5 12-13 0.004147310477449994 0.0 1.658924190979998E-5 14-15 0.005632047628377093 0.0 1.658924190979998E-5 16-17 0.006967481602115991 0.0 1.658924190979998E-5 18-19 0.00852687034163719 0.0 1.658924190979998E-5 20-21 0.010442927782219086 0.0 1.658924190979998E-5 22-23 0.012881546342959682 0.0 1.658924190979998E-5 24-25 0.01551094118566298 0.0 1.658924190979998E-5 26-27 0.018132041407411377 0.0 1.658924190979998E-5 28-29 0.02112639957213027 0.0 1.658924190979998E-5 30-31 0.030623740565490762 0.0 1.658924190979998E-5 32-33 0.03428996302755656 0.0 2.4883862864699968E-5 34-35 0.03792300700580275 0.0 4.147310477449995E-5 36-37 0.041713648782192046 0.0 5.806234668429992E-5 38-39 0.04621762796070274 0.0 8.29462095489999E-5 40-41 0.05108657046122904 0.0 9.953545145879987E-5 42-43 0.05624582469517683 0.0 9.953545145879987E-5 44-45 0.06147143589676382 0.0 9.953545145879987E-5 46-47 0.06681317179171942 0.0 9.953545145879987E-5 48-49 0.072652584943969 0.0 9.953545145879987E-5 50-51 0.0785251765800382 0.0 9.953545145879987E-5 52-53 0.08480420464289748 0.0 9.953545145879987E-5 54-55 0.09158920458400568 0.0 9.953545145879987E-5 56-57 0.09867281087949027 0.0 9.953545145879987E-5 58-59 0.10655270078664526 0.0 9.953545145879987E-5 60-61 0.11445747455666495 0.0 9.953545145879987E-5 62-63 0.12355667374419024 0.0 9.953545145879987E-5 64-65 0.13293789004418213 0.0 9.953545145879987E-5 66-67 0.1427670158757386 0.0 1.0783007241369986E-4 68-69 0.1545619668736064 0.0 1.3271393527839983E-4 70-71 0.16778359267571696 0.0 1.3271393527839983E-4 72-73 0.18166878815421955 0.0 1.3271393527839983E-4 74-75 0.19821655695924506 0.0 1.4100855623329982E-4 76-77 0.215635260964535 0.0 1.493031771881998E-4 78-79 0.23634692948892028 0.0 1.493031771881998E-4 80-81 0.26078288282205564 0.0 1.493031771881998E-4 82-83 0.28604000362972615 0.0 1.493031771881998E-4 84-85 0.314250009497341 0.0 1.493031771881998E-4 86-87 0.3479261705742349 0.0 1.493031771881998E-4 88-89 0.38639662256306107 0.0 1.658924190979998E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28335 0.0 83.01265 3 GATCGGA 28765 0.0 82.1027 1 CGGAAGA 28630 0.0 82.04116 4 ATCGGAA 28980 0.0 81.19784 2 GAAGAGC 29965 0.0 78.28927 6 GGAAGAG 34375 0.0 68.46651 5 AGAGCAC 34550 0.0 67.91354 8 GAGCACA 34605 0.0 67.901665 9 AAGAGCA 36405 0.0 64.37478 7 GCCGTCT 25520 0.0 45.51929 50-51 TATGCCG 25460 0.0 45.325787 48-49 ATGCCGT 25590 0.0 45.206898 48-49 TCGTATG 25750 0.0 45.104477 44-45 CGTATGC 25590 0.0 45.099197 46-47 TCTCGTA 25580 0.0 45.070786 42-43 TGCCGTC 25650 0.0 45.066357 50-51 CTCGTAT 25520 0.0 44.943375 44-45 TCGTATC 25320 0.0 44.900127 38-39 CGTATCT 25470 0.0 44.831512 38-39 AATTCGT 25215 0.0 44.739166 34-35 >>END_MODULE