FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6028003
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2176143.6100512889592125TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC119040.19747833569425896No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA101440.16828126993301099No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG101170.1678333604014464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG283350.083.012653
GATCGGA287650.082.10271
CGGAAGA286300.082.041164
ATCGGAA289800.081.197842
GAAGAGC299650.078.289276
GGAAGAG343750.068.466515
AGAGCAC345500.067.913548
GAGCACA346050.067.9016659
AAGAGCA364050.064.374787
GCCGTCT255200.045.5192950-51
TATGCCG254600.045.32578748-49
ATGCCGT255900.045.20689848-49
TCGTATG257500.045.10447744-45
CGTATGC255900.045.09919746-47
TCTCGTA255800.045.07078642-43
TGCCGTC256500.045.06635750-51
CTCGTAT255200.044.94337544-45
TCGTATC253200.044.90012738-39
CGTATCT254700.044.83151238-39
AATTCGT252150.044.73916634-35
Produced by FastQC (version 0.11.2)