##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_35010-1.341000000357eb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6886504 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68876108980696 33.0 33.0 33.0 33.0 33.0 2 32.721792363730565 33.0 33.0 33.0 33.0 33.0 3 32.749833877973494 33.0 33.0 33.0 33.0 33.0 4 36.68505601681201 37.0 37.0 37.0 37.0 37.0 5 36.68585395434316 37.0 37.0 37.0 37.0 37.0 6 36.560106550435464 37.0 37.0 37.0 37.0 37.0 7 36.51288316974767 37.0 37.0 37.0 37.0 37.0 8 36.445016368247224 37.0 37.0 37.0 37.0 37.0 9 36.464119094391 37.0 37.0 37.0 37.0 37.0 10-11 36.419539725817344 37.0 37.0 37.0 37.0 37.0 12-13 36.39623900603267 37.0 37.0 37.0 37.0 37.0 14-15 38.28468871868803 40.0 37.0 40.0 37.0 40.0 16-17 38.25979982005383 40.0 37.0 40.0 37.0 40.0 18-19 38.246006682055224 40.0 37.0 40.0 37.0 40.0 20-21 38.18485417274135 40.0 37.0 40.0 37.0 40.0 22-23 38.084334518646905 40.0 37.0 40.0 37.0 40.0 24-25 38.07145447094781 40.0 37.0 40.0 37.0 40.0 26-27 38.00276439249872 40.0 37.0 40.0 35.0 40.0 28-29 37.870373995281206 40.0 37.0 40.0 33.0 40.0 30-31 37.8346781618075 40.0 37.0 40.0 33.0 40.0 32-33 37.71845692676574 40.0 37.0 40.0 33.0 40.0 34-35 37.642030194130434 40.0 37.0 40.0 33.0 40.0 36-37 37.52768654458053 40.0 37.0 40.0 33.0 40.0 38-39 37.4542718627623 40.0 37.0 40.0 33.0 40.0 40-41 37.399437145465974 40.0 37.0 40.0 33.0 40.0 42-43 37.273226661888245 40.0 37.0 40.0 33.0 40.0 44-45 37.14521620839834 40.0 37.0 40.0 33.0 40.0 46-47 37.080710255886004 40.0 37.0 40.0 33.0 40.0 48-49 36.90420233546659 37.0 37.0 40.0 33.0 40.0 50-51 36.791802052245956 37.0 37.0 40.0 33.0 40.0 52-53 36.67629692802037 37.0 37.0 40.0 33.0 40.0 54-55 36.501533942331264 37.0 37.0 40.0 33.0 40.0 56-57 36.31752141580111 37.0 37.0 40.0 33.0 40.0 58-59 36.13146641605087 37.0 37.0 40.0 33.0 40.0 60-61 35.87815769801339 37.0 37.0 40.0 30.0 40.0 62-63 35.6506983804845 37.0 35.0 40.0 27.0 40.0 64-65 35.43797244581576 37.0 33.0 40.0 27.0 40.0 66-67 35.105883478757875 37.0 33.0 38.5 27.0 40.0 68-69 34.831835500277066 37.0 33.0 37.0 27.0 40.0 70-71 34.52935756662597 37.0 33.0 37.0 27.0 40.0 72-73 34.22576259303705 37.0 33.0 37.0 27.0 40.0 74-75 33.805216405886064 37.0 33.0 37.0 27.0 38.5 76-77 31.128763883677408 33.0 30.0 35.0 24.5 37.0 78-79 33.49840550444754 37.0 33.0 37.0 27.0 37.0 80-81 33.83899341378441 37.0 33.0 37.0 27.0 37.0 82-83 33.75198881754806 37.0 33.0 37.0 27.0 37.0 84-85 33.590403563259386 37.0 33.0 37.0 27.0 37.0 86-87 33.3954963941065 37.0 33.0 37.0 27.0 37.0 88-89 33.27119420826591 37.0 33.0 37.0 27.0 37.0 90-91 33.12307892364544 37.0 33.0 37.0 27.0 37.0 92-93 32.92783747747769 35.0 33.0 37.0 27.0 37.0 94-95 32.8076147926437 33.0 33.0 37.0 27.0 37.0 96-97 32.64759985618247 33.0 33.0 37.0 27.0 37.0 98-99 32.5596144284531 33.0 33.0 37.0 27.0 37.0 100-101 30.941096091717945 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14571716538750223 2306 2 0.12331075969124328 2306 3 0.11633253636769325 2306 4 0.13145210888542636 2306 5 0.13549160877589372 2306 6 0.14595836186498445 2306 7 0.247272125384562 2306 8 0.2498769883344849 2306 9 0.1823617858961768 2306 10-11 0.20290404534164708 2306 12-13 0.21461066029018383 2306 14-15 0.45350850655021446 2306 16-17 0.434081193161461 2306 18-19 0.4541955224252021 2306 20-21 0.494253641913339 2306 22-23 0.48132219309443514 2306 24-25 0.39617358728425245 2306 26-27 0.4911873152000581 2306 28-29 0.5267855092047427 2306 30-31 0.5908541163369563 2306 32-33 0.6046885261687933 2306 34-35 0.56114092406591 2306 36-37 0.5566024301294519 2306 38-39 0.6716069318629039 2306 40-41 0.6013534677137571 2306 42-43 0.5109883147359469 2306 44-45 0.5396272773303608 2306 46-47 0.6231089734610222 2306 48-49 0.5345495967544736 2306 50-51 0.5583020751832279 2306 52-53 0.5353370795298602 2306 54-55 0.6097616592936674 2306 56-57 0.6949070494109648 2306 58-59 0.7432060853526252 2306 60-61 0.7464901091957472 2306 62-63 0.6187315132818867 2306 64-65 0.634949110119237 2306 66-67 0.6844888591019256 2306 68-69 0.6213083995960034 2306 70-71 0.6127956833808028 2306 72-73 0.598857785078259 2306 74-75 0.5674217587852368 2306 76-77 -0.024492522183926724 2306 78-79 0.446549375918714 2306 80-81 0.6583019413253126 2306 82-83 0.5672131157819038 2306 84-85 0.6494630894230227 2306 86-87 0.7113080501027298 2306 88-89 0.7070220387873647 2306 90-91 0.5469042302481384 2306 92-93 0.5322467835464835 2306 94-95 0.44785026292749563 2306 96-97 0.48099112050831394 2306 98-99 0.6099181140936523 2306 100-101 0.4162800221138454 2307 1 0.144941224433893 2307 2 0.14989511449400794 2307 3 0.14706015471612943 2307 4 0.12838788413156266 2307 5 0.12213742217145551 2307 6 0.1533401052019414 2307 7 0.16440622520234172 2307 8 0.22893156429903883 2307 9 0.24749001253784542 2307 10-11 0.23441392355189805 2307 12-13 0.2732186651145767 2307 14-15 0.47225911943187526 2307 16-17 0.49710396482863217 2307 18-19 0.5570624451693433 2307 20-21 0.49765730478254255 2307 22-23 0.48762460922469586 2307 24-25 0.48602741337007416 2307 26-27 0.49284908318337983 2307 28-29 0.5567382353810117 2307 30-31 0.47770853236234245 2307 32-33 0.47944173478452967 2307 34-35 0.5161862707537281 2307 36-37 0.536055621702495 2307 38-39 0.5743564173045073 2307 40-41 0.6168030409908027 2307 42-43 0.4452584283976364 2307 44-45 0.5898795092683855 2307 46-47 0.6834373731516266 2307 48-49 0.6040608908196745 2307 50-51 0.59209289572361 2307 52-53 0.583109572614454 2307 54-55 0.5874132016578812 2307 56-57 0.5633851102140639 2307 58-59 0.5686738287682473 2307 60-61 0.549998283136155 2307 62-63 0.5092221332428153 2307 64-65 0.47734672938535283 2307 66-67 0.5215744265582316 2307 68-69 0.5900750850533285 2307 70-71 0.40752878161229233 2307 72-73 0.4727311184989844 2307 74-75 0.3839516818309008 2307 76-77 0.25470266915313644 2307 78-79 0.5296629720591994 2307 80-81 0.522180256457915 2307 82-83 0.5244760584004453 2307 84-85 0.5216622268002808 2307 86-87 0.5163146443160826 2307 88-89 0.5408225867756755 2307 90-91 0.41151407044007016 2307 92-93 0.4969820579994746 2307 94-95 0.26446002446081707 2307 96-97 0.3554904453552652 2307 98-99 0.3661918722708677 2307 100-101 0.3516597727660553 2201 1 0.1308234553052401 2201 2 0.1365950858424867 2201 3 0.10446971671512273 2201 4 0.1369598004284569 2201 5 0.12000404695775302 2201 6 0.14043764812955573 2201 7 0.1599029561706189 2201 8 0.1634251381394023 2201 9 0.1231469942761052 2201 10-11 0.148333156403055 2201 12-13 0.2029729456352456 2201 14-15 0.3159056337154098 2201 16-17 0.27497992324919096 2201 18-19 0.3071547410936617 2201 20-21 0.3985591167014846 2201 22-23 0.30789770441429454 2201 24-25 0.3280086229461716 2201 26-27 0.3633058569133212 2201 28-29 0.3797421930189486 2201 30-31 0.3117949448863726 2201 32-33 0.3842320038894087 2201 34-35 0.4479326932450647 2201 36-37 0.36442841984092667 2201 38-39 0.4070451385777787 2201 40-41 0.3963644769336696 2201 42-43 0.3741568825432964 2201 44-45 0.40727533021438234 2201 46-47 0.4911384970575341 2201 48-49 0.4803169368971112 2201 50-51 0.4619025622728685 2201 52-53 0.3122738324312806 2201 54-55 0.42255427342423957 2201 56-57 0.41300411941767123 2201 58-59 0.40604720963504093 2201 60-61 0.3633618008381916 2201 62-63 0.513097526353782 2201 64-65 0.4822991436687758 2201 66-67 0.46342347477328616 2201 68-69 0.3667733465380252 2201 70-71 0.30457445521906834 2201 72-73 0.35634042629697404 2201 74-75 0.3722933013185994 2201 76-77 0.09889349635529854 2201 78-79 0.4960623359823373 2201 80-81 0.5018811555204934 2201 82-83 0.4628171317862737 2201 84-85 0.4803037663782206 2201 86-87 0.5594371785602021 2201 88-89 0.4431300931624733 2201 90-91 0.4175728353327557 2201 92-93 0.4147145884124086 2201 94-95 0.4185296038653945 2201 96-97 0.6108923108957498 2201 98-99 0.5214342054478109 2201 100-101 0.3385758545152484 1101 1 0.08837021223498454 1101 2 0.11259618883295985 1101 3 0.10455092312359682 1101 4 0.1059938489347374 1101 5 0.09950334999932409 1101 6 0.08372471151862015 1101 7 0.0823027699777299 1101 8 0.13795870724959514 1101 9 0.07047591058797309 1101 10-11 0.13060347002169692 1101 12-13 0.1506127737103995 1101 14-15 0.18680591173983174 1101 16-17 0.2327570179824079 1101 18-19 0.16574630321655803 1101 20-21 0.2270403038835198 1101 22-23 0.1507323140290353 1101 24-25 0.20932140928730547 1101 26-27 0.27780044188785524 1101 28-29 0.2653462700360194 1101 30-31 0.22967224478594517 1101 32-33 0.22676202622749742 1101 34-35 0.2686543677667501 1101 36-37 0.19947315925942632 1101 38-39 0.20535637415478902 1101 40-41 0.17865110772784476 1101 42-43 0.23406216703135385 1101 44-45 0.226684926737434 1101 46-47 0.2850286634927244 1101 48-49 0.2978401920344993 1101 50-51 0.2676527855272468 1101 52-53 0.2577773754086934 1101 54-55 0.2668399732539868 1101 56-57 0.1619639943446245 1101 58-59 0.24177271679561585 1101 60-61 0.13695036515442638 1101 62-63 0.1605692495740101 1101 64-65 0.21999713172308333 1101 66-67 0.11747582634391307 1101 68-69 0.2295380579264048 1101 70-71 0.030837012487296533 1101 72-73 0.03429655459804337 1101 74-75 -0.0261114101709623 1101 76-77 -0.11790686995896849 1101 78-79 0.2543345115298479 1101 80-81 0.200030484239484 1101 82-83 0.16325984585117936 1101 84-85 0.22160633234893368 1101 86-87 0.23176173097627384 1101 88-89 0.28474964240366063 1101 90-91 0.21635967230520237 1101 92-93 0.15533777802811244 1101 94-95 0.11320754835664104 1101 96-97 0.1272670392590527 1101 98-99 0.13318193938424372 1101 100-101 0.126328865603341 2304 1 0.0829980047950869 2304 2 0.09785219615532981 2304 3 0.07574578675072274 2304 4 0.07000190228344394 2304 5 0.05449880502455784 2304 6 0.04442170742419904 2304 7 0.12341549322893997 2304 8 0.08155738203893037 2304 9 0.050970170686554184 2304 10-11 0.09101500685332553 2304 12-13 0.11357115056363654 2304 14-15 0.23416675437973566 2304 16-17 0.2843980138123712 2304 18-19 0.26577804714779774 2304 20-21 0.28379577478859375 2304 22-23 0.29510635206188596 2304 24-25 0.28828719515833967 2304 26-27 0.24410193519170065 2304 28-29 0.30535224860768295 2304 30-31 0.2628810442351863 2304 32-33 0.2723808517177915 2304 34-35 0.19249748658042165 2304 36-37 0.24651305736377083 2304 38-39 0.22414641837573868 2304 40-41 0.1913494285290227 2304 42-43 0.23217616003981334 2304 44-45 0.22858869615203758 2304 46-47 0.2984311796915833 2304 48-49 0.33486562813939713 2304 50-51 0.22704737440788847 2304 52-53 0.24664600752095112 2304 54-55 0.35912848226595173 2304 56-57 0.44652541609465146 2304 58-59 0.3724768964394869 2304 60-61 0.34104765597136577 2304 62-63 0.3543015954584021 2304 64-65 0.36334843227903946 2304 66-67 0.3249573116763216 2304 68-69 0.31285926082446736 2304 70-71 0.16667752562046445 2304 72-73 0.18707655103767706 2304 74-75 0.12073440096919086 2304 76-77 0.03214566900414795 2304 78-79 0.3663983475515664 2304 80-81 0.3127234093600393 2304 82-83 0.32686235669373787 2304 84-85 0.4090583816835718 2304 86-87 0.408007896892272 2304 88-89 0.44534679852552017 2304 90-91 0.29176281235501733 2304 92-93 0.3832100130371714 2304 94-95 0.43932461178305715 2304 96-97 0.1719885670007315 2304 98-99 0.2204128445825333 2304 100-101 0.2765874644277879 2202 1 0.10122828834487763 2202 2 0.08521665024544234 2202 3 0.07179691214938089 2202 4 0.11104056971416298 2202 5 0.0745864358318471 2202 6 0.07956433238145877 2202 7 0.1079381496192866 2202 8 0.05537281594402543 2202 9 0.05838319118188906 2202 10-11 0.04992296974654664 2202 12-13 0.10005383581762572 2202 14-15 0.030071071903996938 2202 16-17 0.09607795366419936 2202 18-19 0.13412564769942747 2202 20-21 0.11768025089781986 2202 22-23 0.06261315325681949 2202 24-25 0.11410194928782147 2202 26-27 0.02141297283850463 2202 28-29 0.12343156337405503 2202 30-31 0.16485030275157442 2202 32-33 0.10574136277920587 2202 34-35 0.1144521413377646 2202 36-37 0.22513358466166977 2202 38-39 0.223844563339334 2202 40-41 0.052629501584959826 2202 42-43 0.13676036074500786 2202 44-45 0.18418533199175613 2202 46-47 0.30382842889743245 2202 48-49 0.21289979050921204 2202 50-51 0.20424060674781686 2202 52-53 0.20004000942959976 2202 54-55 0.10469295324729444 2202 56-57 0.1529782363381571 2202 58-59 0.10738909644482675 2202 60-61 0.11249384076855051 2202 62-63 0.20778402184195244 2202 64-65 0.14340309080527192 2202 66-67 0.1226944236560783 2202 68-69 0.1741388180652308 2202 70-71 -0.04162270384163236 2202 72-73 0.07081476947536203 2202 74-75 0.06281843317476188 2202 76-77 0.07824895363260964 2202 78-79 0.2781111826560547 2202 80-81 0.10865537457731023 2202 82-83 0.1836806285451118 2202 84-85 0.28048508930000793 2202 86-87 0.3155465837176479 2202 88-89 0.1569802943796006 2202 90-91 0.07466422040662479 2202 92-93 0.08326689713391744 2202 94-95 0.34533579795711944 2202 96-97 0.1779342652810385 2202 98-99 0.16258294914582905 2202 100-101 0.23048837509603004 1102 1 0.06897860329599581 1102 2 0.04730422374353083 1102 3 0.04135868015854527 1102 4 0.06117759908150333 1102 5 0.013217098971971097 1102 6 -0.03162987342913226 1102 7 -0.02835532559582532 1102 8 0.05479855696193425 1102 9 0.06577355060206003 1102 10-11 -0.03153059518123058 1102 12-13 0.05003549689148912 1102 14-15 -0.01400129737135103 1102 16-17 0.08749949381505928 1102 18-19 0.1107445420330464 1102 20-21 0.05599220400484484 1102 22-23 0.05897905583953644 1102 24-25 -0.020444059774568757 1102 26-27 0.036896465526858435 1102 28-29 -0.01897919667761272 1102 30-31 8.279725461051157E-4 1102 32-33 0.034917284338725096 1102 34-35 0.0073206626280040155 1102 36-37 0.10983772424709315 1102 38-39 0.0816298076798958 1102 40-41 0.10026523241964469 1102 42-43 0.02433798793855857 1102 44-45 0.027078257722521926 1102 46-47 0.04814165319958619 1102 48-49 -0.021568050304352937 1102 50-51 -0.03427962416317598 1102 52-53 -0.0015681492283050602 1102 54-55 -0.015816772078878216 1102 56-57 -0.02549491137335025 1102 58-59 -0.006202411379398143 1102 60-61 -0.06069943328790828 1102 62-63 -0.15365083965929216 1102 64-65 -0.19144958269122725 1102 66-67 -0.20301114089807726 1102 68-69 -0.08855761347589919 1102 70-71 -0.12611281335122015 1102 72-73 -0.04443960054265972 1102 74-75 -0.2222710516722728 1102 76-77 -0.0047376202231426134 1102 78-79 0.028519964154007482 1102 80-81 -0.10203792795573463 1102 82-83 -0.12955159010045492 1102 84-85 -0.08544867528285494 1102 86-87 -0.031185413949557983 1102 88-89 -0.05945508539563349 1102 90-91 -0.14562518151657144 1102 92-93 -0.11852772625744024 1102 94-95 -0.2348654233470171 1102 96-97 -0.31843371609299 1102 98-99 -0.45279757218086303 1102 100-101 -0.4893637687358243 2305 1 0.04976471075504918 2305 2 0.002764005852107232 2305 3 0.030131175117709574 2305 4 0.00643304959681501 2305 5 0.030770843900079115 2305 6 0.02994008068905174 2305 7 -0.014235338236431971 2305 8 0.05640941416782397 2305 9 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90-91 -0.34085853504206653 1213 92-93 -0.21096614015393556 1213 94-95 -0.36445156884138896 1213 96-97 -0.35542132501304735 1213 98-99 -0.2863852508502518 1213 100-101 0.033810366649365875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 16.0 11 15.0 12 48.0 13 183.0 14 588.0 15 1512.0 16 3223.0 17 5075.0 18 6553.0 19 8132.0 20 10676.0 21 14099.0 22 18241.0 23 23266.0 24 29729.0 25 38114.0 26 49073.0 27 64129.0 28 87849.0 29 117785.0 30 131117.0 31 151954.0 32 204531.0 33 291465.0 34 447224.0 35 755279.0 36 1405232.0 37 2234334.0 38 786674.0 39 382.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.05678882140595 27.66581900141097 20.145031908881464 16.132360268301614 2 30.61756734621805 24.649996573007147 30.16797783026046 14.56445825051434 3 35.70485111168163 20.9365013074849 25.458824971277156 17.899822609556317 4 29.61304475128621 21.32143288701334 30.043117671390128 19.022404690310317 5 30.62270311212153 19.656989364105847 31.424069032534995 18.29623849123763 6 27.551381658966584 20.097105875492122 33.734010754949104 18.617501710592197 7 31.935754339211886 21.240646923315516 26.314237238517542 20.50936149895506 8 29.16439168553449 27.711099855601624 23.524026124140786 19.600482334723104 9 28.079036910455578 24.813490270244525 26.629157552220985 20.478315267078912 10-11 22.75647411226364 29.29430520914531 30.868609093961176 17.080611584629878 12-13 22.110159233190018 28.218403706728406 30.76396238207369 18.907474678007883 14-15 21.631737961671117 28.35550520263983 31.331253129309154 18.6815037063799 16-17 21.890889775131186 27.753915484547747 31.93465799192159 18.420536748399478 18-19 20.955632930729436 27.80968398479112 32.50944165573708 18.725241428742365 20-21 21.662994750311622 28.162751375734334 31.90849812909424 18.265755744859803 22-23 21.077121972746312 29.71169890688728 30.71191222338376 18.499266896982654 24-25 21.0630241411317 28.129338195403648 31.415112806149537 19.39252485731512 26-27 21.098898657431985 28.266984234671177 30.66709174931141 19.96702535858543 28-29 21.686590666785545 29.09001551150352 30.413771752335222 18.809622069375713 30-31 20.878520878390187 28.656126094787602 30.97318737399601 19.492165652826206 32-33 20.74169177884861 29.35495478645545 30.351653802723966 19.55169963197197 34-35 21.524734785295742 29.220745935233243 30.515170559889963 18.73934871958105 36-37 21.445444732314307 29.077792604894874 30.7170583090034 18.75970435378742 38-39 21.07212654684604 28.472655519633168 31.560999783981604 18.894218149539192 40-41 20.994027114464732 28.660279545072807 29.909799269189126 20.435894071273342 42-43 20.798168829409214 29.14086040446483 30.768744281040888 19.29222648508507 44-45 20.67577714938517 28.39516795029241 30.95034483419598 19.97871006612644 46-47 21.424777758069247 28.4831975973759 30.071376912164222 20.020647732390636 48-49 20.851241215846443 29.45412613514815 30.296590186203254 19.398042462802152 50-51 21.60334977463996 28.784441139338114 30.041245577822913 19.570963508199014 52-53 20.774102186530904 28.736021626047513 29.46995546830961 21.01992071911197 54-55 21.420475143616724 28.868450391808736 30.01678869053152 19.694285774043017 56-57 20.67601589998553 28.837601314769067 30.21406826431936 20.272314520926045 58-59 20.711316000757567 28.90033062105169 30.314787144180062 20.073566234010688 60-61 21.175704369729274 28.955881782634695 30.2795503107333 19.58886353690273 62-63 20.30307750005355 28.865002354405352 30.293932169164293 20.5379879763768 64-65 21.051769391065974 28.714894898723998 30.214941298648938 20.01839441156109 66-67 20.54271383060737 29.81724705291945 29.58105937664203 20.058979739831152 68-69 20.46814871631722 30.037472525716513 29.49935427262334 19.99502448534293 70-71 20.257295846352605 30.33366209726207 29.35184454141354 20.057197514971783 72-73 20.261828049490187 30.265667803002273 29.344068859047056 20.128435288460484 74-75 20.395391067569538 30.053864139530923 29.37844695011624 20.1722978427833 76-77 20.470672073989064 29.883394533572687 29.384728606552656 20.26120478588559 78-79 20.297059324145888 29.82187681356007 29.629280001765995 20.251783860528043 80-81 20.22254959524621 30.041247258303365 29.5003871014964 20.23581604495402 82-83 20.103803605396724 29.88310495068599 29.512144401748603 20.50094704216868 84-85 20.239583769000916 29.610800722162583 29.645029671866794 20.504585836969706 86-87 20.3362359425104 29.618567306194464 29.733674321222647 20.311522430072486 88-89 20.556944633057203 29.823393097959134 29.35474828487458 20.264913984109082 90-91 20.60058657006105 29.744002281912646 29.276444015585323 20.378967132440977 92-93 20.332501306578326 29.848465965196063 29.23776588033747 20.581266847888134 94-95 20.055269271924775 29.924666946675956 29.20025004737231 20.81981373402696 96-97 20.110709951257988 30.22105675237736 29.059121568866704 20.609111727497947 98-99 20.390529319764727 30.190654579172545 28.89545283948437 20.52336326157836 100-101 20.45761243903151 29.975218263596513 28.675353588551424 20.891815708820552 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 159.0 1 96.0 2 36.0 3 37.0 4 36.0 5 41.5 6 50.0 7 59.0 8 70.5 9 82.5 10 91.5 11 111.5 12 125.5 13 152.5 14 197.0 15 234.5 16 274.0 17 327.5 18 430.0 19 587.5 20 811.0 21 1242.5 22 2076.0 23 3558.0 24 5829.0 25 9607.5 26 16522.0 27 26291.5 28 37733.0 29 51616.5 30 68048.5 31 86874.5 32 112063.0 33 147078.0 34 189642.0 35 236850.0 36 291456.5 37 352594.0 38 409394.0 39 443328.5 40 460872.5 41 456469.0 42 430733.5 43 408917.5 44 378165.0 45 347029.5 46 328807.5 47 300012.5 48 257722.5 49 218459.0 50 179299.0 51 143415.5 52 115773.5 53 92194.5 54 74737.5 55 58705.5 56 41766.5 57 28448.0 58 19960.5 59 14662.5 60 10678.0 61 7486.5 62 5171.5 63 3393.5 64 2277.5 65 1622.0 66 1093.0 67 762.0 68 568.0 69 413.5 70 285.5 71 218.5 72 167.5 73 120.0 74 87.5 75 62.0 76 58.5 77 41.5 78 26.5 79 23.0 80 18.5 81 11.5 82 7.0 83 4.5 84 2.5 85 2.5 86 1.5 87 2.5 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 2.0 95 3.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1504682201593145 2 0.0 3 0.0 4 0.016844541148890644 5 1.742538739540411E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.339865917452455E-4 24-25 0.0 26-27 0.0 28-29 2.904231232567352E-5 30-31 9.511357286658078E-4 32-33 0.0010455232437242468 34-35 1.1616924930269408E-4 36-37 0.0014158127258765841 38-39 0.007500177158105187 40-41 0.01653233629138965 42-43 0.006055322119902929 44-45 0.002192694580588351 46-47 4.864587314550314E-4 48-49 0.006585344319846471 50-51 0.002759019670938984 52-53 0.010651268045440764 54-55 0.02195598811820918 56-57 0.019465609836282677 58-59 0.01858707988843105 60-61 0.02411964038647186 62-63 0.006919330911591717 64-65 0.010411668968753958 66-67 0.028120218909333385 68-69 0.04057211031896591 70-71 0.03831407053564479 72-73 0.02827995162712459 74-75 0.03790747816308536 76-77 0.031009928985737904 78-79 0.012720532798645 80-81 0.01035358434410261 82-83 0.004748418065247621 84-85 0.007551001204675115 86-87 0.023328237375597256 88-89 0.0208959437183221 90-91 0.015276256283304271 92-93 0.030639639503585565 94-95 0.032069973385624985 96-97 0.03338413801836171 98-99 0.023117680611236122 100-101 0.023161244079724634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6886504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.07075238932221 #Duplication Level Percentage of deduplicated Percentage of total 1 91.70673785930448 59.674264316752826 2 4.743757005307253 6.173596749749217 3 1.2175568492509632 2.376820207725982 4 0.46845449862742666 1.2193074674339743 5 0.27474477945789905 0.8938924757181943 6 0.253858661036366 0.9911264444509347 7 0.1778966388209177 0.8103107694926034 8 0.1092597984592584 0.5687693833319721 9 0.0951106912259013 0.5570031814504113 >10 0.792696194604434 10.54072837512398 >50 0.09513528007498537 4.339828603829103 >100 0.05891224759392566 7.822950327700815 >500 0.004996532331631323 2.182739433808055 >1k 8.606355437090022E-4 0.7377135640892056 >5k 0.0 0.0 >10k+ 2.2328360895064037E-5 1.1109486993426017 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 76463 1.1103311636789872 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.293539944215526E-4 0.0 0.0 2 0.0010455232437242468 0.0 1.452115616283676E-5 3 0.0011181290245384306 0.0 1.452115616283676E-5 4 0.0011762136491897776 0.0 1.452115616283676E-5 5 0.0013649886793066556 0.0 2.904231232567352E-5 6 0.0015973271779120436 0.0 2.904231232567352E-5 7 0.0017715810518660847 0.0 2.904231232567352E-5 8 0.0023233849860538817 0.0 4.3563468488510284E-5 9 0.002613808109310617 0.0 4.3563468488510284E-5 10-11 0.004537861300886487 0.0 6.534520273276542E-5 12-13 0.006745077037637675 0.0 7.26057808141838E-5 14-15 0.010230154516718496 0.0 7.26057808141838E-5 16-17 0.014942269691559026 0.0 7.26057808141838E-5 18-19 0.02011180128552891 0.0 7.26057808141838E-5 20-21 0.022783694019490878 0.0 7.26057808141838E-5 22-23 0.024852958772695118 0.0 7.26057808141838E-5 24-25 0.027263470695726016 0.0 7.986635889560219E-5 26-27 0.03021126539678188 0.0 8.712693697702057E-5 28-29 0.03368908229778128 0.0 1.0164809313985732E-4 30-31 0.05314017097790112 0.0 1.1616924930269408E-4 32-33 0.058033800604777114 0.0 1.3069040546553084E-4 34-35 0.06236110514130247 0.0 1.3795098354694923E-4 36-37 0.06702239626957307 0.0 1.452115616283676E-4 38-39 0.07203945572383316 0.0 1.5973271779120437E-4 40-41 0.07764462200268815 0.0 1.5973271779120437E-4 42-43 0.08393954319927789 0.0 1.5973271779120437E-4 44-45 0.09072092312732266 0.0 1.5973271779120437E-4 46-47 0.09784355022519409 0.0 1.7425387395404114E-4 48-49 0.10577936206818439 0.0 1.7425387395404114E-4 50-51 0.11441218940699083 0.0 1.7425387395404114E-4 52-53 0.12358956010190367 1.452115616283676E-5 1.7425387395404114E-4 54-55 0.13332599530908573 1.452115616283676E-5 1.815144520354595E-4 56-57 0.14394822104220079 1.452115616283676E-5 1.9603560819829625E-4 58-59 0.15494799683554966 1.452115616283676E-5 2.0329618627971463E-4 60-61 0.16780648061774162 1.452115616283676E-5 2.1055676436113302E-4 62-63 0.18223324926551993 1.452115616283676E-5 2.3959907668680654E-4 64-65 0.19835899318435013 1.452115616283676E-5 2.6864138901248007E-4 66-67 0.21765034914667877 1.452115616283676E-5 2.904231232567352E-4 68-69 0.23809613702395294 1.452115616283676E-5 3.1220485750099036E-4 70-71 0.26164219174199277 1.452115616283676E-5 3.1946543558240874E-4 72-73 0.2876713641638776 1.452115616283676E-5 3.1946543558240874E-4 74-75 0.3168371063169353 1.452115616283676E-5 3.267260136638271E-4 76-77 0.34963313751070213 1.452115616283676E-5 3.412471698266639E-4 78-79 0.386647564569773 1.452115616283676E-5 3.4850774790808227E-4 80-81 0.42845397316258005 1.452115616283676E-5 3.4850774790808227E-4 82-83 0.477114367464246 1.452115616283676E-5 3.4850774790808227E-4 84-85 0.5314452732474997 1.452115616283676E-5 3.557683259895006E-4 86-87 0.5929568907532763 1.452115616283676E-5 3.63028904070919E-4 88-89 0.6609885073761665 1.452115616283676E-5 3.63028904070919E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 15210 0.0 55.019882 4 TCGGAAG 15200 0.0 54.993584 3 GATCGGA 15820 0.0 53.070145 1 ATCGGAA 15960 0.0 52.404602 2 CGGTCGC 65 4.3743057E-8 51.226746 1 GAAGAGC 17880 0.0 46.71701 6 GCCGTCT 9960 0.0 41.99322 50-51 TATGCCG 9945 0.0 41.863224 48-49 TGCCGTC 10035 0.0 41.5137 50-51 ATGCCGT 10070 0.0 41.390736 48-49 CGTATGC 10155 0.0 41.015392 46-47 CTCGTAT 10125 0.0 40.760483 44-45 TCTCGTA 10195 0.0 40.36445 42-43 TCGTATG 10375 0.0 40.258938 44-45 CCGTCTT 10415 0.0 40.11349 52-53 CATCTCG 10320 0.0 39.64962 40-41 GTATGCC 10695 0.0 39.122112 46-47 CGTCTTC 10740 0.0 38.92174 52-53 ATCTCGT 10570 0.0 38.820084 42-43 AGAGCAC 21725 0.0 38.4488 8 >>END_MODULE