FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6886504
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT764631.1103311636789872TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA152100.055.0198824
TCGGAAG152000.054.9935843
GATCGGA158200.053.0701451
ATCGGAA159600.052.4046022
CGGTCGC654.3743057E-851.2267461
GAAGAGC178800.046.717016
GCCGTCT99600.041.9932250-51
TATGCCG99450.041.86322448-49
TGCCGTC100350.041.513750-51
ATGCCGT100700.041.39073648-49
CGTATGC101550.041.01539246-47
CTCGTAT101250.040.76048344-45
TCTCGTA101950.040.3644542-43
TCGTATG103750.040.25893844-45
CCGTCTT104150.040.1134952-53
CATCTCG103200.039.6496240-41
GTATGCC106950.039.12211246-47
CGTCTTC107400.038.9217452-53
ATCTCGT105700.038.82008442-43
AGAGCAC217250.038.44888
Produced by FastQC (version 0.11.2)