##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5804081 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6757772677535 33.0 33.0 33.0 33.0 33.0 2 32.708530601140815 33.0 33.0 33.0 33.0 33.0 3 32.73892869517155 33.0 33.0 33.0 33.0 33.0 4 36.671995962840626 37.0 37.0 37.0 37.0 37.0 5 36.673249046662164 37.0 37.0 37.0 37.0 37.0 6 36.54206945078816 37.0 37.0 37.0 37.0 37.0 7 36.473650867381075 37.0 37.0 37.0 37.0 37.0 8 36.398527691119405 37.0 37.0 37.0 37.0 37.0 9 36.439226123825634 37.0 37.0 37.0 37.0 37.0 10-11 36.378646852102854 37.0 37.0 37.0 37.0 37.0 12-13 36.34826900244845 37.0 37.0 37.0 37.0 37.0 14-15 38.19803402123437 40.0 37.0 40.0 37.0 40.0 16-17 38.17661572951859 40.0 37.0 40.0 37.0 40.0 18-19 38.1261330605138 40.0 37.0 40.0 37.0 40.0 20-21 38.092695897938015 40.0 37.0 40.0 37.0 40.0 22-23 37.95778048238817 40.0 37.0 40.0 33.0 40.0 24-25 37.95920163760637 40.0 37.0 40.0 33.0 40.0 26-27 37.89399114175009 40.0 37.0 40.0 33.0 40.0 28-29 37.72080110529126 40.0 37.0 40.0 33.0 40.0 30-31 37.695844355032264 40.0 37.0 40.0 33.0 40.0 32-33 37.56136527040198 40.0 37.0 40.0 33.0 40.0 34-35 37.4409958269018 40.0 37.0 40.0 33.0 40.0 36-37 37.23024592523778 40.0 37.0 40.0 33.0 40.0 38-39 37.25817403306398 40.0 37.0 40.0 33.0 40.0 40-41 37.10134102194645 40.0 37.0 40.0 33.0 40.0 42-43 36.96199639529497 38.5 37.0 40.0 33.0 40.0 44-45 36.89722610694096 37.0 37.0 40.0 33.0 40.0 46-47 36.851034814986214 37.0 37.0 40.0 33.0 40.0 48-49 36.62384983944918 37.0 37.0 40.0 33.0 40.0 50-51 36.550090961859425 37.0 37.0 40.0 33.0 40.0 52-53 36.45335376952872 37.0 37.0 40.0 33.0 40.0 54-55 36.20925466064309 37.0 37.0 40.0 33.0 40.0 56-57 36.043126896402725 37.0 37.0 40.0 30.0 40.0 58-59 35.82761836025376 37.0 37.0 40.0 27.0 40.0 60-61 35.59432121984514 37.0 35.0 40.0 27.0 40.0 62-63 35.38785856020962 37.0 33.0 40.0 27.0 40.0 64-65 35.172565648205115 37.0 33.0 40.0 27.0 40.0 66-67 34.743746770591244 37.0 33.0 40.0 27.0 40.0 68-69 34.4972833769894 37.0 33.0 37.0 27.0 40.0 70-71 34.13807508889004 37.0 33.0 37.0 27.0 40.0 72-73 33.682704807186525 37.0 33.0 37.0 27.0 40.0 74-75 33.08074025844918 37.0 33.0 37.0 22.0 40.0 76-77 30.501113612990583 33.0 30.0 35.0 22.0 37.0 78-79 32.68575421328545 37.0 33.0 37.0 22.0 37.0 80-81 32.95778694335934 37.0 33.0 37.0 22.0 37.0 82-83 32.82040231692149 37.0 33.0 37.0 22.0 37.0 84-85 32.6159200741685 37.0 33.0 37.0 22.0 37.0 86-87 32.39130156867211 37.0 33.0 37.0 22.0 37.0 88-89 32.23267947156492 37.0 33.0 37.0 22.0 37.0 90-91 32.065579115798 33.0 33.0 37.0 22.0 37.0 92-93 31.848705936391998 33.0 33.0 37.0 22.0 37.0 94-95 31.697072456431947 33.0 33.0 37.0 15.0 37.0 96-97 31.521780795271464 33.0 33.0 37.0 15.0 37.0 98-99 31.412440574140852 33.0 33.0 37.0 15.0 37.0 100-101 29.8114910870472 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16819137411171425 2306 2 0.1559981171165532 2306 3 0.1011171942062461 2306 4 0.14240968399791143 2306 5 0.17581823478448655 2306 6 0.1858129370047905 2306 7 0.2216800008687585 2306 8 0.24452264846645022 2306 9 0.2348374258255035 2306 10-11 0.19834081297992157 2306 12-13 0.2407557977775241 2306 14-15 0.4382444665219367 2306 16-17 0.43505879184936447 2306 18-19 0.5324840877609702 2306 20-21 0.49550666711348157 2306 22-23 0.48300499438916944 2306 24-25 0.5388779160755988 2306 26-27 0.5288663980732267 2306 28-29 0.45933057382099207 2306 30-31 0.5381382224300211 2306 32-33 0.6176570501784155 2306 34-35 0.5169908398757741 2306 36-37 0.5694821342952565 2306 38-39 0.5387562733128135 2306 40-41 0.5513240954379413 2306 42-43 0.494748929377522 2306 44-45 0.6724309639717205 2306 46-47 0.6748030917169956 2306 48-49 0.6057182674347743 2306 50-51 0.6197727335503131 2306 52-53 0.5566488681489759 2306 54-55 0.5634771840581507 2306 56-57 0.5994989728074458 2306 58-59 0.5621328032799369 2306 60-61 0.5824703660965938 2306 62-63 0.5335364873565993 2306 64-65 0.5046349391057845 2306 66-67 0.6553388910449058 2306 68-69 0.578859240899547 2306 70-71 0.3474449546140761 2306 72-73 0.10676008913608825 2306 74-75 0.25704032604711813 2306 76-77 -0.21100426670893313 2306 78-79 0.2633017188655131 2306 80-81 0.3468424680840556 2306 82-83 0.416965650352644 2306 84-85 0.4472871900819442 2306 86-87 0.4251392948305508 2306 88-89 0.5293369786000781 2306 90-91 0.5480101974294982 2306 92-93 0.43587338658407404 2306 94-95 0.38957920965379955 2306 96-97 0.347707484494979 2306 98-99 0.2547978889480085 2306 100-101 0.24286943031147246 2307 1 0.11621527036186308 2307 2 0.14113768087197087 2307 3 0.11282106728135233 2307 4 0.08289111782544722 2307 5 0.0806816204376517 2307 6 0.15658030588282656 2307 7 0.10158641295655002 2307 8 0.19790636599767453 2307 9 0.20833667455362104 2307 10-11 0.1839143179935192 2307 12-13 0.18408634585012607 2307 14-15 0.41253034458030413 2307 16-17 0.4210080969524199 2307 18-19 0.487771526405794 2307 20-21 0.43155085624017175 2307 22-23 0.3936628320025548 2307 24-25 0.436319914760837 2307 26-27 0.3906952730196167 2307 28-29 0.46730136364529784 2307 30-31 0.4292460181922735 2307 32-33 0.36285611949949015 2307 34-35 0.40404008002880687 2307 36-37 0.41349919464099827 2307 38-39 0.45806673809286025 2307 40-41 0.4316232148438175 2307 42-43 0.37984648441498337 2307 44-45 0.3804337035336971 2307 46-47 0.5373282161870279 2307 48-49 0.393510519551171 2307 50-51 0.45368370317035556 2307 52-53 0.3912262780429643 2307 54-55 0.3982553796031496 2307 56-57 0.4120551277871911 2307 58-59 0.456268864251534 2307 60-61 0.4113141243942664 2307 62-63 0.3903890888899255 2307 64-65 0.44194569304868025 2307 66-67 0.5062911652542823 2307 68-69 0.43892881201141165 2307 70-71 0.20242003303078349 2307 72-73 0.2537892559767485 2307 74-75 0.20654303028447174 2307 76-77 0.18945220876619828 2307 78-79 0.39656199983935636 2307 80-81 0.458487694415048 2307 82-83 0.5231112999224621 2307 84-85 0.5228273743950282 2307 86-87 0.43233950898440554 2307 88-89 0.2721912615326687 2307 90-91 0.2849846386899131 2307 92-93 0.37854054753274724 2307 94-95 0.29369795499692586 2307 96-97 0.3263338585109743 2307 98-99 0.25750626282586 2307 100-101 0.24149531582347095 2201 1 0.10711197716882026 2201 2 0.1459649756287078 2201 3 0.10389990570364915 2201 4 0.12030478911344744 2201 5 0.11432743272552415 2201 6 0.14341817347846586 2201 7 0.08067684276803533 2201 8 0.16241933278839582 2201 9 0.14161774541818062 2201 10-11 0.1618401096712958 2201 12-13 0.18957178309153022 2201 14-15 0.2763907278756008 2201 16-17 0.3171960947957686 2201 18-19 0.33638638169872337 2201 20-21 0.32903281343475754 2201 22-23 0.3008185363025859 2201 24-25 0.3403526327001103 2201 26-27 0.31090835565249364 2201 28-29 0.30828375659291396 2201 30-31 0.23974121056318864 2201 32-33 0.38321277946747756 2201 34-35 0.4436994335362954 2201 36-37 0.39418744532869 2201 38-39 0.3394897616330823 2201 40-41 0.3157047203854617 2201 42-43 0.2937208847315489 2201 44-45 0.2571924960016787 2201 46-47 0.41584529304334694 2201 48-49 0.27797395156353133 2201 50-51 0.345350509309867 2201 52-53 0.2696007638364577 2201 54-55 0.41642326227380977 2201 56-57 0.3322784915614605 2201 58-59 0.2725259788611538 2201 60-61 0.26178466593545835 2201 62-63 0.30718318392831634 2201 64-65 0.15966978307800872 2201 66-67 0.2576969021311797 2201 68-69 0.3034263869770726 2201 70-71 0.12877433431365404 2201 72-73 0.18509858922082145 2201 74-75 0.16597236118540337 2201 76-77 0.007086697452756141 2201 78-79 0.3791203423280862 2201 80-81 0.48468715094913506 2201 82-83 0.4307248138850923 2201 84-85 0.4124586041988607 2201 86-87 0.48625055678812856 2201 88-89 0.43033574862923984 2201 90-91 0.33542382872725085 2201 92-93 0.30307595351230887 2201 94-95 0.25354125127431715 2201 96-97 0.3893032195141295 2201 98-99 0.46107638917630567 2201 100-101 0.2252407117251174 1101 1 0.09708343860335589 1101 2 0.07904124430040582 1101 3 0.06357161621300378 1101 4 0.11343419667082344 1101 5 0.08915030767746401 1101 6 0.1154407805419666 1101 7 0.11529528000870215 1101 8 0.16877548508988127 1101 9 0.09675630528197843 1101 10-11 0.13236459288631153 1101 12-13 0.13445615061647231 1101 14-15 0.23534721904169942 1101 16-17 0.2826851091209974 1101 18-19 0.30229170913529657 1101 20-21 0.2972343078836417 1101 22-23 0.24969070623732392 1101 24-25 0.24516338027010676 1101 26-27 0.23147474442970406 1101 28-29 0.3085594337599247 1101 30-31 0.30287103498217505 1101 32-33 0.2352262210605076 1101 34-35 0.27788244236662507 1101 36-37 0.3124783456236244 1101 38-39 0.34572389963731354 1101 40-41 0.226902171825202 1101 42-43 0.23366588093865204 1101 44-45 0.3280707356609014 1101 46-47 0.3484046807394563 1101 48-49 0.2981165950256326 1101 50-51 0.32471078579881407 1101 52-53 0.2525547988100527 1101 54-55 0.3078715382493584 1101 56-57 0.3267150071029974 1101 58-59 0.3525042801171736 1101 60-61 0.23547378699920074 1101 62-63 0.2357275086591244 1101 64-65 0.10545405061101576 1101 66-67 0.18850952996645987 1101 68-69 0.27864994272984944 1101 70-71 -2.7381704221340897E-4 1101 72-73 -0.0395409763216108 1101 74-75 0.046344706366561184 1101 76-77 0.11288910943599362 1101 78-79 0.3445917771602822 1101 80-81 0.3176747816777592 1101 82-83 0.26002308220903814 1101 84-85 0.396452160395917 1101 86-87 0.2879962744949225 1101 88-89 0.47785594310614954 1101 90-91 0.3973574797164403 1101 92-93 0.28759236935809085 1101 94-95 0.29897990761963555 1101 96-97 0.2688586256333245 1101 98-99 0.17668637564475986 1101 100-101 0.19505433618875045 2304 1 0.10378573941341074 2304 2 0.11921523175792714 2304 3 0.09443385581683117 2304 4 0.08962354614097023 2304 5 0.08570683197259399 2304 6 0.09001992258117042 2304 7 0.11396999632273008 2304 8 0.1336046272593805 2304 9 0.06347666985094946 2304 10-11 0.1368243504692046 2304 12-13 0.12613488671620843 2304 14-15 0.1846542190349254 2304 16-17 0.18892455868189018 2304 18-19 0.2701160685259225 2304 20-21 0.25119733815476764 2304 22-23 0.2752339610181451 2304 24-25 0.2112250378310634 2304 26-27 0.1758501714635301 2304 28-29 0.11733864990952725 2304 30-31 0.17249632403618875 2304 32-33 0.2419695640233286 2304 34-35 0.22389872163880398 2304 36-37 0.23308223595065414 2304 38-39 0.22426859962308043 2304 40-41 0.2760980322745823 2304 42-43 0.28183216967007496 2304 44-45 0.34833062760148437 2304 46-47 0.2991775240199246 2304 48-49 0.3937806674807973 2304 50-51 0.2760690074410377 2304 52-53 0.32824267831967546 2304 54-55 0.26199941465280574 2304 56-57 0.3970436153336294 2304 58-59 0.3923063084073277 2304 60-61 0.28553968897021775 2304 62-63 0.34104771823208324 2304 64-65 0.4541899001887941 2304 66-67 0.4383342083132433 2304 68-69 0.4384645937621059 2304 70-71 0.18526894783862957 2304 72-73 0.1714112328927726 2304 74-75 0.31085713340925025 2304 76-77 0.019406681470833576 2304 78-79 0.31549325566799524 2304 80-81 0.33878123642272584 2304 82-83 0.23389231226867224 2304 84-85 0.39219868197574925 2304 86-87 0.2788257116467534 2304 88-89 0.33986753497360667 2304 90-91 0.2981374890870754 2304 92-93 0.41275298098258517 2304 94-95 0.37360801359855245 2304 96-97 0.08177322303717105 2304 98-99 0.14748338558712248 2304 100-101 0.24310456031799532 2202 1 0.04594062528843068 2202 2 0.051441152261752165 2202 3 0.016815978845308166 2202 4 0.06101818194463249 2202 5 0.053397124673935537 2202 6 0.03903335609036418 2202 7 0.04284168283121659 2202 8 0.056703081346974216 2202 9 0.059795114915353054 2202 10-11 0.04560090213619361 2202 12-13 0.07768747443914492 2202 14-15 0.08665301747704746 2202 16-17 0.06017590525538452 2202 18-19 0.1153401061286985 2202 20-21 0.13544760746947304 2202 22-23 0.05634049915034467 2202 24-25 0.019275590731837156 2202 26-27 -0.025535533220242712 2202 28-29 0.037781707114731944 2202 30-31 0.11720797316287701 2202 32-33 0.06875705401034082 2202 34-35 0.0599253170406584 2202 36-37 0.1881590481853621 2202 38-39 0.1502242064328314 2202 40-41 0.06519792270723457 2202 42-43 0.14096377938933813 2202 44-45 0.11805194443525835 2202 46-47 0.20856152994945631 2202 48-49 0.11935451495200766 2202 50-51 0.17159872873484971 2202 52-53 0.1330042542480001 2202 54-55 0.06398060405700079 2202 56-57 0.13456513749109433 2202 58-59 0.10697198988860634 2202 60-61 0.08335881537778533 2202 62-63 0.1321264962339086 2202 64-65 0.12034705567314319 2202 66-67 0.08230650803057671 2202 68-69 0.07587919568275936 2202 70-71 -0.13354579127138777 2202 72-73 -0.06688220963960845 2202 74-75 -0.0854891638589308 2202 76-77 -0.012728316549583951 2202 78-79 0.1430386248363007 2202 80-81 0.00637128420881794 2202 82-83 -0.08989254902274268 2202 84-85 0.06259535720524667 2202 86-87 -0.011052659390642816 2202 88-89 0.060673476470171295 2202 90-91 -0.10506620489776708 2202 92-93 -0.009006035305194615 2202 94-95 0.13717594995742033 2202 96-97 0.07333400529278222 2202 98-99 -0.1897962137094389 2202 100-101 -0.032469056969706145 1102 1 0.08222746110153878 1102 2 0.06241618317211106 1102 3 0.09273460702722502 1102 4 0.09062544669015438 1102 5 0.06380542701973013 1102 6 0.036371283143452615 1102 7 0.008029398644204377 1102 8 0.08011222031780107 1102 9 0.07689197973249406 1102 10-11 0.005250549966447693 1102 12-13 0.05860088529150431 1102 14-15 -0.024313388631867383 1102 16-17 0.06945821758201731 1102 18-19 0.09495047734633033 1102 20-21 0.041649997407738226 1102 22-23 0.020725741825636135 1102 24-25 0.03657835097701678 1102 26-27 0.00358472931331022 1102 28-29 0.032760104591893935 1102 30-31 0.022515621953239418 1102 32-33 -0.043189337928417615 1102 34-35 0.025244565899555482 1102 36-37 -0.022737510538142658 1102 38-39 0.019000322022925786 1102 40-41 -0.010504525887398586 1102 42-43 -0.03572822528465025 1102 44-45 -0.052920350464262356 1102 46-47 0.010016072807964349 1102 48-49 0.020585140985247108 1102 50-51 0.006785313869166032 1102 52-53 0.00896864983272394 1102 54-55 -0.012191905864419539 1102 56-57 -0.10811921715765749 1102 58-59 -0.04372104281540601 1102 60-61 -0.0018606096045772347 1102 62-63 -0.12400174356567106 1102 64-65 -0.144140932120159 1102 66-67 -0.08767592207187391 1102 68-69 -0.13779802218397208 1102 70-71 -0.23679496815275058 1102 72-73 -0.11788621756748086 1102 74-75 -0.28655690270226586 1102 76-77 -0.06681512179964244 1102 78-79 0.049033035501885536 1102 80-81 -0.07446087599715412 1102 82-83 -0.10654377980313967 1102 84-85 -0.02838902399322052 1102 86-87 -0.02133604682675383 1102 88-89 0.025282674560436647 1102 90-91 -0.11893156692332241 1102 92-93 -0.14353483944077894 1102 94-95 -0.27217842866326336 1102 96-97 -0.3648284836512623 1102 98-99 -0.29921673510280655 1102 100-101 -0.25963471181402653 2305 1 0.04481571924659988 2305 2 0.016672094953747774 2305 3 0.034865450519625085 2305 4 0.04198376949636895 2305 5 0.008524216593102096 2305 6 0.034339335717639585 2305 7 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-0.24561880021154536 1213 92-93 -0.15103067955490346 1213 94-95 -0.2627521133241473 1213 96-97 -0.2545616608753498 1213 98-99 -0.2122287173536641 1213 100-101 0.0660646755697627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 15.0 10 19.0 11 44.0 12 74.0 13 229.0 14 626.0 15 1502.0 16 2863.0 17 4702.0 18 7240.0 19 9957.0 20 13470.0 21 17453.0 22 22428.0 23 28443.0 24 36170.0 25 45422.0 26 58623.0 27 81302.0 28 122310.0 29 164925.0 30 143780.0 31 142834.0 32 185588.0 33 259080.0 34 381057.0 35 606831.0 36 1047716.0 37 1662856.0 38 752186.0 39 4331.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.19304275686333 27.137955321995598 19.210151161743376 16.4588507593977 2 29.498244424914127 27.587261445868865 28.107688366168563 14.806805763048448 3 33.61226350907232 21.690961928339732 27.67501004896382 17.02176451362412 4 29.204745599271575 22.297316974276377 27.91917613545798 20.57876129099407 5 33.01165096417703 20.51974227046577 29.253235962563508 17.215370802793693 6 30.450453741083212 21.2841791835779 30.773261089912424 17.492105985426463 7 30.803153849851512 25.068206318967633 24.97120560515954 19.157434226021312 8 28.465643398153816 30.54455993980787 22.765154380168024 18.224642281870292 9 30.968330731428455 25.284381799633742 24.61364684607262 19.133640622865187 10-11 26.10091933589486 29.90453613586716 27.793538718704998 16.20100580953298 12-13 24.091117956486137 29.55064720840388 27.147553592032914 19.21068124307707 14-15 23.27102257876828 29.820621042332114 27.68881929800773 19.219537080891875 16-17 24.77469732073002 27.866582151420697 27.644255826202286 19.714464701646996 18-19 22.022470051675707 28.249485146744163 29.89120930600383 19.836835495576302 20-21 23.697351914971552 28.045766418490714 30.096220228490957 18.160661438046784 22-23 21.61162000292726 32.49249383255051 27.559206179880448 18.336679984641787 24-25 21.198058745217374 28.340326401371723 30.098477261085776 20.363137592325124 26-27 21.341302783334694 28.14366305363416 27.843934293818435 22.671099869212714 28-29 23.084189190132143 30.699276620492512 27.703390883976116 18.513143305399232 30-31 20.884319147860246 28.405057559629533 30.053792846670447 20.65683044583978 32-33 20.95420932388241 30.67237018480295 27.648035327238706 20.72538516407593 34-35 22.710210249014914 30.590370205734942 28.13658346642959 18.562836078820553 36-37 20.83598631942191 30.246832590899253 29.996108714437963 18.92107237524088 38-39 20.689268080411622 28.61915581425422 29.89167101090544 20.799905094428716 40-41 22.592852603470927 28.27647308990801 30.104067152193903 19.02660715442716 42-43 20.649379526756427 30.357188548001375 29.80155694015711 19.19187498508509 44-45 20.335646493986395 28.286265066966738 30.27777483438688 21.100313604659988 46-47 22.58905084934117 28.095819174775578 27.925042093397597 21.39008788248566 48-49 20.334872025183074 30.558943936060533 29.870405106914255 19.235778931842134 50-51 22.363775501105756 28.11810839861442 30.077794573840748 19.440321526439067 52-53 20.477082674784555 28.30799552096367 27.746220565400364 23.468701238851413 54-55 22.19105915926939 28.270079495968332 30.262209171074012 19.276652173688262 56-57 20.407104695708878 28.076717148209568 29.964204592158616 21.551973563922942 58-59 20.205070949313253 28.5876516625148 29.929548703639625 21.277728684532317 60-61 22.158105672621467 28.192118377710845 30.157914038155248 19.49186191151244 62-63 20.214409453893076 28.378883884002892 29.818294035731235 21.588412626372797 64-65 22.04002672193013 28.391680292128523 30.16616946438796 19.40212352155339 66-67 20.275291874393364 32.04290877580958 27.84286279559693 19.838936554200124 68-69 20.14365282707971 32.48834205040302 27.788863350991537 19.579141771525734 70-71 20.05110705231475 32.161489460678574 28.07614833592701 19.711255151079666 72-73 20.081439385779344 32.285705668701716 27.754044610274065 19.87881033524487 74-75 19.92920817971019 31.987206351529053 28.20411925904458 19.879466209716178 76-77 20.251781763745974 31.48887778031795 27.971598978444252 20.28774147749183 78-79 20.1270595022456 31.565576674413926 28.103727781775472 20.203636041565 80-81 20.069687425798907 31.09340661802234 28.360419926919782 20.476486029258968 82-83 20.227580068829003 31.152476130523716 27.97437921528529 20.645564585361996 84-85 20.076854877318482 30.95354995907328 28.45690365855854 20.512691505049695 86-87 20.360156977362877 30.481026444800502 28.307969629903766 20.850846947932855 88-89 20.286551112431532 30.92059210660097 28.20710909800902 20.58574768295848 90-91 20.3078955653368 30.43476762437902 28.413561624691614 20.843775185592563 92-93 20.40055691262147 30.52406470382284 28.094504525022764 20.980873858532924 94-95 20.12695991623903 30.48520179758627 28.468397899081815 20.919440387092884 96-97 20.354076558831263 30.275362335076323 28.199818982189743 21.170742123902667 98-99 20.257895741359782 30.56815905265321 28.170960836123328 21.002984369863682 100-101 20.38298363303152 30.188766386722033 28.24410372694554 21.184146253300913 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25.0 1 21.0 2 22.5 3 47.5 4 51.5 5 34.0 6 32.5 7 37.5 8 44.0 9 49.0 10 64.5 11 80.0 12 88.0 13 117.5 14 162.0 15 240.5 16 378.5 17 570.0 18 900.5 19 1499.0 20 2473.0 21 3936.0 22 6204.5 23 9529.0 24 13947.0 25 19713.0 26 27124.0 27 36177.5 28 46689.0 29 58807.0 30 72604.0 31 88555.5 32 107520.5 33 129320.5 34 154008.5 35 181883.0 36 214364.0 37 250096.0 38 282682.5 39 307710.5 40 324990.0 41 330077.5 42 321809.5 43 307210.0 44 288707.5 45 270380.0 46 254949.0 47 237445.5 48 216171.5 49 195035.0 50 175092.5 51 155041.0 52 134889.0 53 114043.0 54 94322.0 55 77548.0 56 63410.5 57 51441.5 58 41557.0 59 33198.0 60 25727.5 61 19675.5 62 14642.5 63 10694.0 64 7895.0 65 5759.0 66 4143.5 67 2921.0 68 2038.5 69 1456.5 70 1077.0 71 769.5 72 541.5 73 378.0 74 290.5 75 224.5 76 168.5 77 133.0 78 103.0 79 85.5 80 65.5 81 42.0 82 36.0 83 29.0 84 16.0 85 15.0 86 14.0 87 6.5 88 5.0 89 4.5 90 1.5 91 2.0 92 1.5 93 1.0 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14760303999892488 2 0.0 3 0.0 4 0.015402955265441678 5 2.0675107738847893E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.5319975720531815E-4 24-25 0.0 26-27 0.0 28-29 7.753165402067959E-5 30-31 9.734529893707548E-4 32-33 0.0010509846433914346 34-35 1.89521820939439E-4 36-37 0.001490330682841952 38-39 0.007193214567474161 40-41 0.015592477086381118 42-43 0.00560812297416249 44-45 0.002024437632762189 46-47 4.0488752655243787E-4 48-49 0.005883791077347129 50-51 0.002437939787539147 52-53 0.010346168497648465 54-55 0.021898384946729726 56-57 0.01898664060684198 58-59 0.0183491581182275 60-61 0.023552393565837555 62-63 0.007219058452147722 64-65 0.010475387921016264 66-67 0.027308371471728255 68-69 0.038696909984543636 70-71 0.036905067313843486 72-73 0.027670185857158094 74-75 0.03686199417272088 76-77 0.030228730439840517 78-79 0.012697962002942412 80-81 0.010251407587178745 82-83 0.004781118664608575 84-85 0.007158756054576081 86-87 0.02297521347479472 88-89 0.020795712533991168 90-91 0.015713081881524395 92-93 0.029884145310859717 94-95 0.031977499969418066 96-97 0.0325546800604609 98-99 0.023233652321530317 100-101 0.022854608679651438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5804081.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.25369289131048 #Duplication Level Percentage of deduplicated Percentage of total 1 96.86860306496033 84.52143342640305 2 2.0663188866206896 3.6058790709743245 3 0.3767985389980094 0.9863119201088035 4 0.17243962819646902 0.6018397744378589 5 0.1490943542848937 0.6504516500301178 6 0.05756208353195838 0.3013502615208874 7 0.043449283385288084 0.26537773001932113 8 0.04496260801451827 0.3138522873032922 9 0.03845702763480306 0.3019965910883772 >10 0.15903419928694426 2.4752435815613967 >50 0.01344182615290747 0.8214971386414818 >100 0.009570309577426061 1.401680358967927 >500 1.8077039407606398E-4 0.08673130991379525 >1k 6.773374887869553E-5 0.09678861861618433 >5k 0.0 0.0 >10k+ 1.968521278075438E-5 3.5695662804132082 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 207822 3.5806185337523724 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0016195501062097515 0.0 1.7229256449039908E-5 2 0.0018263011835982304 0.0 1.7229256449039908E-5 3 0.0021191985432319088 0.0 3.4458512898079816E-5 4 0.0023776373899675072 0.0 3.4458512898079816E-5 5 0.002791139544744465 0.0 3.4458512898079816E-5 6 0.003394163520460862 0.0 5.1687769347119725E-5 7 0.004031646009075338 0.0 5.1687769347119725E-5 8 0.004789733292833095 0.0 5.1687769347119725E-5 9 0.0055995083459379705 0.0 5.1687769347119725E-5 10-11 0.007727321517394399 0.0 6.030239757163968E-5 12-13 0.00946747641874743 0.0 6.891702579615963E-5 14-15 0.0114402262821625 0.0 9.47609104697195E-5 16-17 0.013344059119781409 0.0 1.0337553869423945E-4 18-19 0.015635550227503715 0.0 1.119901669187594E-4 20-21 0.018133792412614504 0.0 1.2060479514327936E-4 22-23 0.021596872958871527 0.0 1.292194233677993E-4 24-25 0.02465506597857611 1.7229256449039908E-5 1.7229256449039908E-4 26-27 0.02848857553848749 1.7229256449039908E-5 1.9813644916395894E-4 28-29 0.03274420188140034 1.7229256449039908E-5 2.1536570561299885E-4 30-31 0.0485262007887209 1.7229256449039908E-5 2.670534749601186E-4 32-33 0.053531299787166996 1.7229256449039908E-5 3.015119878581984E-4 34-35 0.058381335477571725 1.7229256449039908E-5 3.2735587253175826E-4 36-37 0.06381716588724382 1.7229256449039908E-5 3.2735587253175826E-4 38-39 0.06966649845169287 1.7229256449039908E-5 3.3597050075627824E-4 40-41 0.07555028952904 1.7229256449039908E-5 3.531997572053181E-4 42-43 0.08283826500698388 1.7229256449039908E-5 3.876582701033979E-4 44-45 0.09012624048492776 1.7229256449039908E-5 4.0488752655243787E-4 46-47 0.09793970828456736 1.7229256449039908E-5 4.479606676750376E-4 48-49 0.10652849262441375 2.5843884673559862E-5 4.479606676750376E-4 50-51 0.1158753642480179 3.4458512898079816E-5 4.479606676750376E-4 52-53 0.12565296728284805 3.4458512898079816E-5 4.479606676750376E-4 54-55 0.13586991635712872 3.4458512898079816E-5 4.479606676750376E-4 56-57 0.14736183040863832 3.4458512898079816E-5 4.651899241240775E-4 58-59 0.15994780224466199 4.307314112259977E-5 4.910338087976374E-4 60-61 0.17398964625062951 5.1687769347119725E-5 5.168776934711972E-4 62-63 0.18847945092427207 5.1687769347119725E-5 5.341069499202372E-4 64-65 0.20445097165253207 5.1687769347119725E-5 5.341069499202372E-4 66-67 0.22177498901204168 5.1687769347119725E-5 5.341069499202372E-4 68-69 0.24026198118186154 5.1687769347119725E-5 5.599508345937971E-4 70-71 0.26185885414073307 5.1687769347119725E-5 5.857947192673569E-4 72-73 0.28591951077181726 5.1687769347119725E-5 6.202532321654367E-4 74-75 0.3131331213330758 5.1687769347119725E-5 6.633263732880364E-4 76-77 0.34346522731161055 6.891702579615963E-5 6.891702579615963E-4 78-79 0.3763731071292768 6.891702579615963E-5 7.408580273087161E-4 80-81 0.41394150081640835 6.891702579615963E-5 7.408580273087161E-4 82-83 0.456118720603658 6.891702579615963E-5 7.49472655533236E-4 84-85 0.502525722849147 6.891702579615963E-5 7.839311684313159E-4 86-87 0.5540153557471028 6.891702579615963E-5 7.925457966558358E-4 88-89 0.61175920873606 6.891702579615963E-5 8.270043095539156E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28455 0.0 77.90033 3 CGGAAGA 28580 0.0 77.426674 4 GATCGGA 28770 0.0 77.34363 1 ATCGGAA 28870 0.0 76.87924 2 GAAGAGC 30900 0.0 71.7105 6 GAGCACA 32170 0.0 68.67286 9 AGAGCAC 32390 0.0 68.32371 8 AAGAGCA 35255 0.0 62.757908 7 GGAAGAG 37530 0.0 59.269985 5 GCCGTCT 24585 0.0 44.787384 50-51 TCTCGTA 24445 0.0 44.772484 42-43 ATGCCGT 24605 0.0 44.61411 48-49 CGTCTTC 24730 0.0 44.56435 52-53 TATGCCG 24460 0.0 44.54845 48-49 TGCCGTC 24535 0.0 44.5108 50-51 CGTATGC 24570 0.0 44.420403 46-47 CTCGTAT 24495 0.0 44.380764 44-45 TATCTCG 24635 0.0 44.23839 40-41 TCGTATG 24970 0.0 44.183193 44-45 ATCTCGT 24660 0.0 44.02584 42-43 >>END_MODULE