FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5804081
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2078223.5806185337523724TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG284550.077.900333
CGGAAGA285800.077.4266744
GATCGGA287700.077.343631
ATCGGAA288700.076.879242
GAAGAGC309000.071.71056
GAGCACA321700.068.672869
AGAGCAC323900.068.323718
AAGAGCA352550.062.7579087
GGAAGAG375300.059.2699855
GCCGTCT245850.044.78738450-51
TCTCGTA244450.044.77248442-43
ATGCCGT246050.044.6141148-49
CGTCTTC247300.044.5643552-53
TATGCCG244600.044.5484548-49
TGCCGTC245350.044.510850-51
CGTATGC245700.044.42040346-47
CTCGTAT244950.044.38076444-45
TATCTCG246350.044.2383940-41
TCGTATG249700.044.18319344-45
ATCTCGT246600.044.0258442-43
Produced by FastQC (version 0.11.2)