##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_35008-1.341000000357b4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2404685 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6634473953969 33.0 33.0 33.0 33.0 33.0 2 32.6991655871767 33.0 33.0 33.0 33.0 33.0 3 32.731047933513125 33.0 33.0 33.0 33.0 33.0 4 36.66081544984063 37.0 37.0 37.0 37.0 37.0 5 36.666532206921076 37.0 37.0 37.0 37.0 37.0 6 36.521535668912975 37.0 37.0 37.0 37.0 37.0 7 36.46917662812385 37.0 37.0 37.0 37.0 37.0 8 36.39006481098356 37.0 37.0 37.0 37.0 37.0 9 36.41234090951622 37.0 37.0 37.0 37.0 37.0 10-11 36.36626294088415 37.0 37.0 37.0 37.0 37.0 12-13 36.3354245982322 37.0 37.0 37.0 37.0 37.0 14-15 38.14343687426836 40.0 37.0 40.0 37.0 40.0 16-17 38.10915712452982 40.0 37.0 40.0 37.0 40.0 18-19 38.106779058379786 40.0 37.0 40.0 37.0 40.0 20-21 38.05901770918021 40.0 37.0 40.0 37.0 40.0 22-23 37.96898575073243 40.0 37.0 40.0 33.0 40.0 24-25 37.94252324108979 40.0 37.0 40.0 33.0 40.0 26-27 37.83282903997821 40.0 37.0 40.0 33.0 40.0 28-29 37.716467853377885 40.0 37.0 40.0 33.0 40.0 30-31 37.68800404210947 40.0 37.0 40.0 33.0 40.0 32-33 37.5755300590306 40.0 37.0 40.0 33.0 40.0 34-35 37.50141349074826 40.0 37.0 40.0 33.0 40.0 36-37 37.40820980710571 40.0 37.0 40.0 33.0 40.0 38-39 37.29366715390997 40.0 37.0 40.0 33.0 40.0 40-41 37.21214920041503 40.0 37.0 40.0 33.0 40.0 42-43 37.08265115805189 38.5 37.0 40.0 33.0 40.0 44-45 36.9459403206657 37.0 37.0 40.0 33.0 40.0 46-47 36.883306545347935 37.0 37.0 40.0 33.0 40.0 48-49 36.67998532032262 37.0 37.0 40.0 33.0 40.0 50-51 36.51664646305025 37.0 37.0 40.0 33.0 40.0 52-53 36.38634831589168 37.0 37.0 40.0 33.0 40.0 54-55 36.214178156390545 37.0 37.0 40.0 33.0 40.0 56-57 36.02064137298648 37.0 37.0 40.0 33.0 40.0 58-59 35.76655112831826 37.0 37.0 40.0 27.0 40.0 60-61 35.490343225827914 37.0 33.0 40.0 27.0 40.0 62-63 35.23874020921659 37.0 33.0 40.0 27.0 40.0 64-65 34.97247269392872 37.0 33.0 37.0 27.0 40.0 66-67 34.59614086668316 37.0 33.0 37.0 27.0 40.0 68-69 34.297654370530864 37.0 33.0 37.0 27.0 40.0 70-71 33.950746355551765 37.0 33.0 37.0 27.0 38.5 72-73 33.60280369362307 37.0 33.0 37.0 27.0 37.0 74-75 33.15141837704314 37.0 33.0 37.0 27.0 37.0 76-77 30.519759136851604 33.0 30.0 35.0 24.5 37.0 78-79 32.92714783849028 35.0 33.0 37.0 27.0 37.0 80-81 33.33411299193034 37.0 33.0 37.0 27.0 37.0 82-83 33.29280155197043 37.0 33.0 37.0 27.0 37.0 84-85 33.14520072275579 37.0 33.0 37.0 27.0 37.0 86-87 32.97271763245497 37.0 33.0 37.0 27.0 37.0 88-89 32.873693643866034 35.0 33.0 37.0 27.0 37.0 90-91 32.74184435799283 33.0 33.0 37.0 27.0 37.0 92-93 32.52053553791869 33.0 33.0 37.0 27.0 37.0 94-95 32.39343281968324 33.0 33.0 37.0 24.5 37.0 96-97 32.22504153350647 33.0 33.0 37.0 22.0 37.0 98-99 32.12630095002048 33.0 33.0 37.0 22.0 37.0 100-101 30.541662421481398 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1524337872316721 2306 2 0.10438920404823193 2306 3 0.12917990763195775 2306 4 0.06964702743066908 2306 5 0.10296628528912777 2306 6 0.07560181189698767 2306 7 0.21152369848699237 2306 8 0.2956528890391823 2306 9 0.19523718030328752 2306 10-11 0.1803417354437329 2306 12-13 0.21949101890227496 2306 14-15 0.47851401937408866 2306 16-17 0.40629215035856703 2306 18-19 0.4573710030649707 2306 20-21 0.347156926897199 2306 22-23 0.4212298047430778 2306 24-25 0.3773176115966095 2306 26-27 0.4730853726471551 2306 28-29 0.46130872762801545 2306 30-31 0.40264752794282543 2306 32-33 0.39098903169325183 2306 34-35 0.46638408630849426 2306 36-37 0.5000220184722934 2306 38-39 0.5084372014088601 2306 40-41 0.37828375086496635 2306 42-43 0.4736956214509931 2306 44-45 0.6552010501283547 2306 46-47 0.5616387127733304 2306 48-49 0.5717416544892373 2306 50-51 0.5220061569990762 2306 52-53 0.36872774813172526 2306 54-55 0.6825651866216518 2306 56-57 0.5257655496705596 2306 58-59 0.43968756919935714 2306 60-61 0.5087574060247064 2306 62-63 0.47799433182187556 2306 64-65 0.44411896471626733 2306 66-67 0.43610003758887217 2306 68-69 0.4069800933117804 2306 70-71 0.35135198637645715 2306 72-73 0.25110563928394924 2306 74-75 0.22757828125513413 2306 76-77 -0.22492729015921142 2306 78-79 0.42430504667908764 2306 80-81 0.3824663920722031 2306 82-83 0.24040932120658454 2306 84-85 0.39294239745385795 2306 86-87 0.38654925474676105 2306 88-89 0.43590902039378676 2306 90-91 0.239871744078755 2306 92-93 0.31365459129115436 2306 94-95 0.30515588964127005 2306 96-97 0.18086017963828738 2306 98-99 0.336330740364005 2306 100-101 0.35827815855438416 2307 1 0.09781584654650288 2307 2 0.17988557360656898 2307 3 0.08235051569322849 2307 4 0.12192703253671766 2307 5 0.10582213727227696 2307 6 0.14704624723558624 2307 7 0.14948310176199442 2307 8 0.19912667622367763 2307 9 0.10032686666833968 2307 10-11 0.16339672933079896 2307 12-13 0.22047475541321404 2307 14-15 0.38753743315608347 2307 16-17 0.3685869221752611 2307 18-19 0.4658960594036188 2307 20-21 0.4061451680622383 2307 22-23 0.43512870075856824 2307 24-25 0.48536091428249506 2307 26-27 0.41153298829712526 2307 28-29 0.4849351600096199 2307 30-31 0.3933012694470932 2307 32-33 0.32621429334005825 2307 34-35 0.35392463230815707 2307 36-37 0.3773212245673747 2307 38-39 0.3449553651941315 2307 40-41 0.3856986381587362 2307 42-43 0.30292609211819155 2307 44-45 0.32681143197593343 2307 46-47 0.4290895861174846 2307 48-49 0.40458017455225814 2307 50-51 0.6103628478544678 2307 52-53 0.4250581641739757 2307 54-55 0.34106695195495007 2307 56-57 0.4234168901753108 2307 58-59 0.37199421958111145 2307 60-61 0.35219584693790296 2307 62-63 0.32082711519908 2307 64-65 0.2869892208685769 2307 66-67 0.14260138127728084 2307 68-69 0.2047475994193917 2307 70-71 0.22037077546962536 2307 72-73 0.47004657335531874 2307 74-75 0.24665755993772365 2307 76-77 0.2509790863970913 2307 78-79 0.39401181155083265 2307 80-81 0.5197489700041515 2307 82-83 0.3996011339840706 2307 84-85 0.30880413570925924 2307 86-87 0.42198281018499273 2307 88-89 0.2892235178183711 2307 90-91 0.2545371563722014 2307 92-93 0.13315143245806382 2307 94-95 0.19545281720883168 2307 96-97 0.3198217206894043 2307 98-99 0.2305833306315037 2307 100-101 0.3083750850861122 2201 1 0.12891482021977652 2201 2 0.1341152506146841 2201 3 0.07901724034128677 2201 4 0.12077675655464759 2201 5 0.0971392369383608 2201 6 0.11821640335328709 2201 7 0.18517728770606823 2201 8 0.16259783880480683 2201 9 0.09438723427410167 2201 10-11 0.17856424037485397 2201 12-13 0.18469261543977922 2201 14-15 0.3214145197146365 2201 16-17 0.19451243142489005 2201 18-19 0.3000536169132033 2201 20-21 0.3834551326505675 2201 22-23 0.38926944179441136 2201 24-25 0.35136807068243314 2201 26-27 0.2643922096668945 2201 28-29 0.253117095514483 2201 30-31 0.3445992857540787 2201 32-33 0.3087041470642262 2201 34-35 0.3347481289114711 2201 36-37 0.2562764204762118 2201 38-39 0.1707829607077187 2201 40-41 0.17597316695238874 2201 42-43 0.33198346620084607 2201 44-45 0.27802458035985467 2201 46-47 0.35368703160703063 2201 48-49 0.3786100289834238 2201 50-51 0.333588631318662 2201 52-53 0.21010609140471814 2201 54-55 0.3583638763414214 2201 56-57 0.42904412444612916 2201 58-59 0.4456037463351308 2201 60-61 0.3509441502808315 2201 62-63 0.28950103215812817 2201 64-65 0.3331981917867921 2201 66-67 0.3675353245748454 2201 68-69 0.27410475225627806 2201 70-71 0.4087589077637048 2201 72-73 0.29537912936393695 2201 74-75 0.17901151276294058 2201 76-77 -0.2125627760736677 2201 78-79 0.26363139564470117 2201 80-81 0.22635340580500696 2201 82-83 0.33266545236698875 2201 84-85 0.42710006075839857 2201 86-87 0.45731199392109545 2201 88-89 0.4301693588973663 2201 90-91 0.3005752692238133 2201 92-93 0.19567580568391207 2201 94-95 0.10624119953363476 2201 96-97 0.20242034563266031 2201 98-99 0.2593212927127908 2201 100-101 0.03148079095998213 1101 1 0.09278841179392572 1101 2 0.10836073306153082 1101 3 0.0986490249772487 1101 4 0.04617639024971254 1101 5 0.06689104665914414 1101 6 0.018654764865516427 1101 7 0.05163484492563697 1101 8 0.09364778834443399 1101 9 0.09812244398575132 1101 10-11 0.1688485420074386 1101 12-13 0.13156308594641786 1101 14-15 0.3282570397430362 1101 16-17 0.23521635362869375 1101 18-19 0.3064662893194736 1101 20-21 0.30485816982512404 1101 22-23 0.3269041585310575 1101 24-25 0.3050011137128763 1101 26-27 0.2682291707480431 1101 28-29 0.39147845200559317 1101 30-31 0.3391960803782794 1101 32-33 0.3476167779393293 1101 34-35 0.42185560742947104 1101 36-37 0.30792276801735596 1101 38-39 0.2554844865629491 1101 40-41 0.33838989695409083 1101 42-43 0.18890598564781413 1101 44-45 0.20139100365385332 1101 46-47 0.3006530238074774 1101 48-49 0.3404488527375946 1101 50-51 0.2616828593164868 1101 52-53 0.3076882504441798 1101 54-55 0.30380463627879095 1101 56-57 0.2257337267020958 1101 58-59 0.2895174051067215 1101 60-61 0.1111867753830964 1101 62-63 0.28647756289327475 1101 64-65 0.17770237189917282 1101 66-67 0.1910496507523618 1101 68-69 0.26210821387940086 1101 70-71 0.13933815339150613 1101 72-73 0.10971117315440893 1101 74-75 0.26651561396609935 1101 76-77 0.11435840399303387 1101 78-79 0.3859482224140365 1101 80-81 0.4433023806080385 1101 82-83 0.34668845232961587 1101 84-85 0.38413217058343463 1101 86-87 0.23737718747711511 1101 88-89 0.3854893461888267 1101 90-91 0.2896149335688847 1101 92-93 0.30369293106235773 1101 94-95 0.20456754524740717 1101 96-97 0.22587403630019764 1101 98-99 0.23606020285179596 1101 100-101 0.11797049330607834 2304 1 0.08437622051870619 2304 2 0.059367188031210105 2304 3 0.053368713447731864 2304 4 0.02626882476510417 2304 5 0.048672423177364976 2304 6 0.10366484567458656 2304 7 0.07219481760989765 2304 8 0.12304879317346717 2304 9 0.07863355726478005 2304 10-11 0.08327548053076583 2304 12-13 0.11584027567700161 2304 14-15 0.11689539884747546 2304 16-17 0.16660384692170993 2304 18-19 0.2074467292980131 2304 20-21 0.2915092274971016 2304 22-23 0.3049844960755692 2304 24-25 0.2487640195873908 2304 26-27 0.19061913546520515 2304 28-29 0.30841746311075013 2304 30-31 0.18958345985570446 2304 32-33 0.17772364264511253 2304 34-35 0.18216506387874887 2304 36-37 0.2395754922747102 2304 38-39 0.2108017068611474 2304 40-41 0.21080872205205736 2304 42-43 0.19621253979964592 2304 44-45 0.22770984175930664 2304 46-47 0.2043254056424999 2304 48-49 0.2616224133769194 2304 50-51 0.3337027201362872 2304 52-53 0.33096252161794126 2304 54-55 0.32481783888679416 2304 56-57 0.3549327088655758 2304 58-59 0.2499471808900111 2304 60-61 0.16195157547415562 2304 62-63 0.2613009454925006 2304 64-65 0.33958167046998256 2304 66-67 0.2310832669987235 2304 68-69 0.17968134039355732 2304 70-71 0.08760490460690562 2304 72-73 0.1051941170758397 2304 74-75 0.09871993733244011 2304 76-77 -0.013261386911477047 2304 78-79 0.1896183685857622 2304 80-81 0.3835136304657709 2304 82-83 0.2521930618608579 2304 84-85 0.4001659412937997 2304 86-87 0.16874029171594174 2304 88-89 0.39420233579909336 2304 90-91 0.11422714760722386 2304 92-93 0.17809539923464968 2304 94-95 0.06713725729397169 2304 96-97 -0.11588876388842095 2304 98-99 -0.162343127067075 2304 100-101 -0.03568664537008459 2202 1 0.06838869604182918 2202 2 0.04890477762450729 2202 3 0.07333192593219451 2202 4 -0.03340655265859738 2202 5 0.04123527123346804 2202 6 0.07268820188095049 2202 7 0.12223431598059165 2202 8 0.06346354469703641 2202 9 0.049322319419374594 2202 10-11 0.0681731760244162 2202 12-13 0.1397417971095294 2202 14-15 0.11175793240711585 2202 16-17 0.07546240456044728 2202 18-19 0.1417153620022944 2202 20-21 0.11897831815890925 2202 22-23 0.06904344739382395 2202 24-25 0.023752095550960917 2202 26-27 0.025986153580433324 2202 28-29 0.04795167577966453 2202 30-31 0.1590548989937517 2202 32-33 0.08156176174016139 2202 34-35 0.0391836402233281 2202 36-37 0.23829878196730903 2202 38-39 0.12763888880144947 2202 40-41 0.16697286666080657 2202 42-43 0.20937788705926152 2202 44-45 0.22363587360766957 2202 46-47 0.34551181932086905 2202 48-49 0.24257865588945293 2202 50-51 0.2739977655128314 2202 52-53 0.24450708584099345 2202 54-55 0.1529907271678823 2202 56-57 0.2614334746122893 2202 58-59 0.2425209748389392 2202 60-61 0.0861972637239532 2202 62-63 0.08523125229422845 2202 64-65 0.13667031915991856 2202 66-67 0.20166967341087627 2202 68-69 0.17833767382687427 2202 70-71 -0.20621790886949043 2202 72-73 0.1496344419313118 2202 74-75 0.14121736621208214 2202 76-77 0.07642075833446782 2202 78-79 0.35812235382231705 2202 80-81 0.2149977677710524 2202 82-83 0.39612596307208037 2202 84-85 0.4614281238396174 2202 86-87 0.3441534642362498 2202 88-89 0.2973550556904314 2202 90-91 0.24579573131116206 2202 92-93 0.2165642737732867 2202 94-95 0.1336623182649319 2202 96-97 0.17502314077145797 2202 98-99 0.0938990594285869 2202 100-101 0.07624815064504986 1102 1 0.04487326303060257 1102 2 0.009304560958568686 1102 3 0.010059786339965626 1102 4 0.055209894996131936 1102 5 0.06579551642251147 1102 6 -0.05223088113654484 1102 7 0.010287157946343939 1102 8 0.031094331748548143 1102 9 -0.04073843335358163 1102 10-11 -0.07258476351966436 1102 12-13 0.03964259944299897 1102 14-15 -0.023554016928869714 1102 16-17 -0.11443004088889808 1102 18-19 0.018127350743085913 1102 20-21 0.012556403601593047 1102 22-23 -0.021067922606818 1102 24-25 0.008827374056622261 1102 26-27 -0.02970973650234754 1102 28-29 -5.222853874400357E-4 1102 30-31 -0.10350724827375046 1102 32-33 -0.1147911359247189 1102 34-35 -0.19215433241755164 1102 36-37 -0.0799299135238769 1102 38-39 -0.10425970492693892 1102 40-41 -0.16508522175035267 1102 42-43 -0.23530928032405285 1102 44-45 0.049530054159120596 1102 46-47 -0.02727811353769738 1102 48-49 -0.08637786693689264 1102 50-51 -0.19398270390044559 1102 52-53 -0.15325851646570499 1102 54-55 -0.11845121446863516 1102 56-57 -0.09679543464090301 1102 58-59 -0.20472226171129648 1102 60-61 -0.15106292455600823 1102 62-63 -0.22808726961819303 1102 64-65 -0.25268526101636013 1102 66-67 -0.3015517803885075 1102 68-69 -0.38316195531622554 1102 70-71 -0.3167524227606009 1102 72-73 -0.39682219080097525 1102 74-75 -0.45032721748238913 1102 76-77 -0.2498713018592973 1102 78-79 -0.03898441787543305 1102 80-81 -0.15249227918801722 1102 82-83 -0.16378376079985202 1102 84-85 -0.04794473475923411 1102 86-87 -0.0055104454384178325 1102 88-89 -0.10708546664709218 1102 90-91 -0.2570487148899758 1102 92-93 -0.283938268582844 1102 94-95 -0.08670512650885343 1102 96-97 -0.28889494321119 1102 98-99 -0.32832837688344796 1102 100-101 -0.5131182789609028 2305 1 0.006172331445824852 2305 2 0.062026816426715925 2305 3 0.06378502808819064 2305 4 0.007160560654206449 2305 5 0.03767568772759233 2305 6 0.028361370922468154 2305 7 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0.1280257929122257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 24.0 10 27.0 11 18.0 12 34.0 13 98.0 14 244.0 15 495.0 16 918.0 17 1537.0 18 2402.0 19 3047.0 20 4345.0 21 5723.0 22 7392.0 23 9407.0 24 12024.0 25 15256.0 26 19925.0 27 26351.0 28 36285.0 29 48934.0 30 51515.0 31 58898.0 32 79151.0 33 111950.0 34 170124.0 35 289548.0 36 544140.0 37 748527.0 38 156288.0 39 51.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.64498685167338 25.498174560799246 24.719728653856247 15.137109933671129 2 28.68637680195119 23.457167986659375 34.00141806515198 13.855037146237448 3 36.85135475124601 19.427118312793567 26.896703726267678 16.824823209692745 4 27.81611671822737 19.27653365697758 34.744826530620735 18.162523094174322 5 32.2601357434639 18.108748637554307 32.74073963179347 16.89037598718833 6 25.802922212264807 19.393849922131174 38.01508305661656 16.788144808987457 7 31.472396592485087 20.911429147684622 29.673782636811058 17.94239162301923 8 27.477611412721416 28.19612548005248 26.630140746085246 17.696122361140855 9 30.424192773689697 24.486408822777204 25.95762022884494 19.13177817468816 10-11 24.254632103581134 28.774891513857327 29.06087491708893 17.90960146547261 12-13 23.69141904241096 26.902026668773665 28.974522650575857 20.43203163823952 14-15 22.941695066089736 27.461996061854254 29.773567016054077 19.82274185600193 16-17 22.663301014477987 27.618024814060888 30.650833685077256 19.067840486383872 18-19 20.917895691119625 27.906524139336337 31.484456384100206 19.691123785443832 20-21 21.813314425797973 28.068354067164726 31.030280473326027 19.088051033711277 22-23 21.136119084575792 29.485804229465284 30.30830537566888 19.069771310290047 24-25 21.524669551313373 27.385977789190687 31.195603582173963 19.893749077321978 26-27 21.379473818816187 27.575046211873904 30.26165589255973 20.783824076750175 28-29 21.42046077146503 28.791157426265272 30.004060823779582 19.78432097849011 30-31 20.149031680824265 28.70538889858073 30.5527629414014 20.592816479193598 32-33 19.953473652605215 29.49083425134291 30.424417765679042 20.131274330372833 34-35 21.028056718506498 28.922841165101836 30.639907763290996 19.40919435310067 36-37 20.168940014110216 29.349647067825728 31.305032914483476 19.176380003580576 38-39 20.75838072207783 29.71412042837332 29.752860428078037 19.77463842147082 40-41 20.633579769369145 28.53646109534059 31.156959998336298 19.672999136953965 42-43 20.645583341668267 29.43841406573444 30.762223187526423 19.15377940507087 44-45 20.1088980466678 28.856713982935865 31.252940935514317 19.781447034882017 46-47 21.12871479822828 28.78898710968186 30.2852196535662 19.79707843852366 48-49 20.70069607980745 30.134339763883904 30.08389347223738 19.08107068407126 50-51 21.385449099878944 29.328031319696535 29.697361871163896 19.589157709260625 52-53 20.23240362870734 28.92144212523719 29.41168152634452 21.43447271971095 54-55 21.45496065088265 29.04486880854533 29.841501256176894 19.65866928439512 56-57 20.701241177709836 28.978539090931587 29.445782260565174 20.874437470793403 58-59 20.550306650223256 28.77805761155975 30.279031946111086 20.392603792105916 60-61 20.816828481118225 29.131634368249788 30.406128553370067 19.64540859726192 62-63 20.19532926541191 29.21494412153193 29.990987746329388 20.598738866726777 64-65 21.185992526669153 28.973516027693208 29.829149816597916 20.011341629039727 66-67 20.88076031534909 30.172264795897675 28.900261127016297 20.046713761736935 68-69 21.034659353524074 30.32636230092744 28.721571537985675 19.917406807562813 70-71 20.542379438879088 31.17484690651313 28.799880187705927 19.482893466901853 72-73 20.192673474451674 31.526934581161488 28.635875711091284 19.644516233295555 74-75 20.936732062796594 30.58802435017458 28.52319760575199 19.952045981276843 76-77 21.31145017781381 29.629846097143876 29.09634226444402 19.9623614605983 78-79 21.18126137381905 29.46132885925859 29.655392128625767 19.702017638296592 80-81 20.372262322913066 30.102059383218542 29.417677802276504 20.108000491591888 82-83 19.534940395874777 29.954293349735412 29.685992131244898 20.824774123144916 84-85 19.679631059813044 29.491821407197893 30.108475368567973 20.720072164421094 86-87 19.661464561865586 29.675790142719222 30.751477377520235 19.911267917894953 88-89 19.943934376724883 30.126319230895614 30.256906087689313 19.672840304690187 90-91 20.349832279316807 29.56914604904972 29.721727159104848 20.35929451252862 92-93 19.667278034559686 30.443604327802458 29.597894919878083 20.291222717759773 94-95 19.329999136811256 30.921292786342065 29.544059545463526 20.20464853138315 96-97 19.865847920763414 31.362978136222935 29.003939115722808 19.767234827290846 98-99 20.29729081056888 30.41418806469177 28.704030694524345 20.584490430215006 100-101 20.262739747679994 30.272327575090785 28.32678893053089 21.138143746698333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 145.0 1 101.0 2 92.5 3 187.5 4 203.0 5 110.5 6 72.5 7 78.0 8 71.5 9 77.5 10 82.5 11 76.5 12 73.5 13 78.0 14 91.0 15 111.0 16 107.5 17 126.5 18 180.0 19 237.5 20 326.0 21 469.0 22 708.5 23 1076.0 24 1656.0 25 2429.0 26 3565.5 27 5171.0 28 7037.0 29 8840.0 30 10912.5 31 13643.0 32 19360.5 33 29934.5 34 45975.0 35 69643.5 36 103545.0 37 145699.5 38 186409.0 39 203787.0 40 200614.5 41 181296.0 42 155376.5 43 145684.0 44 137425.0 45 127462.5 46 124477.0 47 110614.5 48 82656.0 49 61872.0 50 49455.5 51 40291.0 52 33483.5 53 26382.5 54 19408.0 55 13654.5 56 9385.5 57 6285.5 58 4480.5 59 3279.5 60 2443.0 61 1822.0 62 1300.0 63 891.0 64 619.0 65 426.5 66 305.0 67 229.0 68 174.0 69 125.0 70 90.0 71 73.5 72 50.0 73 32.5 74 22.5 75 20.0 76 15.0 77 8.5 78 10.5 79 10.0 80 5.0 81 2.0 82 2.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1504978822590069 2 0.0 3 0.0 4 0.016592609842869234 5 8.317097665598612E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.1189116245994796E-4 24-25 0.0 26-27 0.0 28-29 2.079274416399653E-5 30-31 0.0011851864173478023 32-33 0.0010812226965278198 34-35 1.0396372081998266E-4 36-37 0.0018505542305956913 38-39 0.008046791991466658 40-41 0.017050050214477155 42-43 0.006092274040050984 44-45 0.0021000671605636498 46-47 4.990258599359168E-4 48-49 0.006133859528378976 50-51 0.002661471252991556 52-53 0.010292408361178283 54-55 0.023495800905316083 56-57 0.019815485188288694 58-59 0.01996103439743667 60-61 0.02470178006682788 62-63 0.007526973387366744 64-65 0.011165703616066136 66-67 0.02838209578385527 68-69 0.04058743660812123 70-71 0.03846657670339358 72-73 0.030003929828646997 74-75 0.038424991215065596 76-77 0.03185448405924268 78-79 0.01307863607915382 80-81 0.010458750314490256 82-83 0.004636781948571226 84-85 0.007319045945726779 86-87 0.02470178006682788 88-89 0.02237299272046027 90-91 0.016218340447917295 92-93 0.03268619382580255 94-95 0.0335802818248544 96-97 0.03518132312548213 98-99 0.025283976903419787 100-101 0.02474336555515587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2404685.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.334104857728505 #Duplication Level Percentage of deduplicated Percentage of total 1 87.51108391765347 34.4217015103046 2 5.464900043123635 4.299139026664602 3 1.8254334928952656 2.154053772610559 4 1.0577958646932573 1.6642981383966475 5 0.6218663587134452 1.2230278280564228 6 0.4034237436949901 0.952098710195768 7 0.4423546349674492 1.2179736517278303 8 0.3393818257423138 1.067942425644441 9 0.23032337195663657 0.8153607297355154 >10 1.6198170464634898 12.809532787612774 >50 0.23627815493525647 6.5553702037644355 >100 0.20530143762494785 17.5676872109921 >500 0.034350755146609895 9.054121856695469 >1k 0.0074769779254565336 4.531324113807359 >5k 1.0618723188256056E-4 0.21472824506695146 >10k+ 1.0618723183321569E-4 1.4516397887247463 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 34755 1.445303646839399 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5141 0.21379099549421235 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4032 0.16767268893846804 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3915 0.16280718680409284 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 3801 0.15806644113470164 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3224 0.13407161436944964 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3213 0.13361417399784173 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2998 0.12467329400732322 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 2782 0.1156908285284767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.732952548878544E-4 0.0 4.158548832799306E-5 2 8.732952548878544E-4 0.0 4.158548832799306E-5 3 8.732952548878544E-4 0.0 4.158548832799306E-5 4 9.564662315438404E-4 0.0 4.158548832799306E-5 5 0.0012059791615117988 0.0 4.158548832799306E-5 6 0.001372321114823771 0.0 4.158548832799306E-5 7 0.0016218340447917295 0.0 4.158548832799306E-5 8 0.0018713469747596878 0.0 4.158548832799306E-5 9 0.00203768892807166 0.0 4.158548832799306E-5 10-11 0.0034931810195514175 0.0 8.317097665598612E-5 12-13 0.005260564273491123 0.0 8.317097665598612E-5 14-15 0.0072566677132347894 0.0 8.317097665598612E-5 16-17 0.00894087999051851 0.0 8.317097665598612E-5 18-19 0.01126966733688612 0.0 8.317097665598612E-5 20-21 0.01359845468325373 0.0 8.317097665598612E-5 22-23 0.01653023161037724 0.0 8.317097665598612E-5 24-25 0.019420423049172762 0.0 8.317097665598612E-5 26-27 0.022289821743804283 0.0 8.317097665598612E-5 28-29 0.025741417275027705 0.0 8.317097665598612E-5 30-31 0.03794675809929367 0.0 8.317097665598612E-5 32-33 0.04206372144376498 0.0 8.317097665598612E-5 34-35 0.046222270276564295 0.0 8.317097665598612E-5 36-37 0.05046399008601958 0.0 8.317097665598612E-5 38-39 0.055350284964558766 0.0 8.317097665598612E-5 40-41 0.060735605703033865 0.0 8.317097665598612E-5 42-43 0.06668233053393688 0.0 8.317097665598612E-5 44-45 0.07331521592225176 0.0 8.317097665598612E-5 46-47 0.08042633442633859 0.0 8.317097665598612E-5 48-49 0.08714239079130946 0.0 8.317097665598612E-5 50-51 0.09435747301621626 0.0 8.317097665598612E-5 52-53 0.10153096975279506 0.0 8.317097665598612E-5 54-55 0.10922428509347379 0.0 8.317097665598612E-5 56-57 0.11718790610828445 0.0 8.317097665598612E-5 58-59 0.12662781195873887 0.0 8.317097665598612E-5 60-61 0.13710735501739313 0.0 8.317097665598612E-5 62-63 0.14781561826185136 0.0 8.317097665598612E-5 64-65 0.15958431145867338 0.0 8.317097665598612E-5 66-67 0.17226788539871127 0.0 8.317097665598612E-5 68-69 0.185907925570293 0.0 8.317097665598612E-5 70-71 0.20108662881001044 0.0 1.247564649839792E-4 72-73 0.2175960676762237 0.0 1.247564649839792E-4 74-75 0.23487483807650483 0.0 1.455492091479757E-4 76-77 0.2541912974048576 0.0 1.6634195331197225E-4 78-79 0.2778534402634857 0.0 1.6634195331197225E-4 80-81 0.30272156228362546 0.0 1.6634195331197225E-4 82-83 0.329918471650133 0.0 1.6634195331197225E-4 84-85 0.36046301282704385 0.0 1.6634195331197225E-4 86-87 0.3952492738134101 0.0 1.6634195331197225E-4 88-89 0.4335495085634917 0.0 1.6634195331197225E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCGTC 15 6.6247425E-4 94.9786 8 TCGGAAG 5320 0.0 69.90514 3 CGGAAGA 5325 0.0 69.75031 4 GATCGGA 5530 0.0 67.351364 1 ATCGGAA 5570 0.0 66.76757 2 GAAGAGC 5650 0.0 65.56045 6 GGAAGAG 6475 0.0 57.500557 5 ACGGCGC 45 1.5037876E-5 52.852707 1 TTGTGTT 26655 0.0 48.415104 1 AACGCGG 40 5.351796E-4 47.567436 1 GCCGTCT 4285 0.0 43.457676 50-51 TGTACCG 55 4.9672708E-5 43.17838 2 CGTCTTC 4275 0.0 43.170856 52-53 TCGTATG 4320 0.0 42.765026 44-45 TCTCGTA 4300 0.0 42.743927 42-43 CGTATGC 4295 0.0 42.740177 46-47 ATGCCGT 4330 0.0 42.7291 48-49 TATGCCG 4305 0.0 42.701385 48-49 TGCCGTC 4330 0.0 42.67691 50-51 CTCGTAT 4290 0.0 42.676613 44-45 >>END_MODULE