FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l02n01_35008-1.341000000357b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l02n01_35008-1.341000000357b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404685
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT347551.445303646839399TruSeq Adapter, Index 2 (97% over 37bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC51410.21379099549421235No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG40320.16767268893846804No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA39150.16280718680409284No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC38010.15806644113470164No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA32240.13407161436944964No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA32130.13361417399784173No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG29980.12467329400732322No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC27820.1156908285284767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGTC156.6247425E-494.97868
TCGGAAG53200.069.905143
CGGAAGA53250.069.750314
GATCGGA55300.067.3513641
ATCGGAA55700.066.767572
GAAGAGC56500.065.560456
GGAAGAG64750.057.5005575
ACGGCGC451.5037876E-552.8527071
TTGTGTT266550.048.4151041
AACGCGG405.351796E-447.5674361
GCCGTCT42850.043.45767650-51
TGTACCG554.9672708E-543.178382
CGTCTTC42750.043.17085652-53
TCGTATG43200.042.76502644-45
TCTCGTA43000.042.74392742-43
CGTATGC42950.042.74017746-47
ATGCCGT43300.042.729148-49
TATGCCG43050.042.70138548-49
TGCCGTC43300.042.6769150-51
CTCGTAT42900.042.67661344-45