##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l02n01_35005-1.3410000003578e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2624215 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.68295585537008 33.0 33.0 33.0 33.0 33.0 2 32.71392930838365 33.0 33.0 33.0 33.0 33.0 3 32.7390278616653 33.0 33.0 33.0 33.0 33.0 4 36.675103983476966 37.0 37.0 37.0 37.0 37.0 5 36.671822240174684 37.0 37.0 37.0 37.0 37.0 6 36.5419110095781 37.0 37.0 37.0 37.0 37.0 7 36.50357726024735 37.0 37.0 37.0 37.0 37.0 8 36.41938560674335 37.0 37.0 37.0 37.0 37.0 9 36.432685965136244 37.0 37.0 37.0 37.0 37.0 10-11 36.39024489228207 37.0 37.0 37.0 37.0 37.0 12-13 36.36486835110691 37.0 37.0 37.0 37.0 37.0 14-15 38.1990421897596 40.0 37.0 40.0 37.0 40.0 16-17 38.174870961411315 40.0 37.0 40.0 37.0 40.0 18-19 38.161421606080296 40.0 37.0 40.0 37.0 40.0 20-21 38.09716677177747 40.0 37.0 40.0 37.0 40.0 22-23 38.009821603793895 40.0 37.0 40.0 35.0 40.0 24-25 37.98488843330291 40.0 37.0 40.0 35.0 40.0 26-27 37.88786265607048 40.0 37.0 40.0 33.0 40.0 28-29 37.774146173236566 40.0 37.0 40.0 33.0 40.0 30-31 37.73876378269311 40.0 37.0 40.0 33.0 40.0 32-33 37.62424858481489 40.0 37.0 40.0 33.0 40.0 34-35 37.55975139232113 40.0 37.0 40.0 33.0 40.0 36-37 37.46050514153757 40.0 37.0 40.0 33.0 40.0 38-39 37.35470092961133 40.0 37.0 40.0 33.0 40.0 40-41 37.274478844149584 40.0 37.0 40.0 33.0 40.0 42-43 37.14400458803871 40.0 37.0 40.0 33.0 40.0 44-45 37.00169174400726 37.0 37.0 40.0 33.0 40.0 46-47 36.92177374948318 37.0 37.0 40.0 33.0 40.0 48-49 36.735628178331424 37.0 37.0 40.0 33.0 40.0 50-51 36.58096611748656 37.0 37.0 40.0 33.0 40.0 52-53 36.44752811793241 37.0 37.0 40.0 33.0 40.0 54-55 36.269358074700435 37.0 37.0 40.0 33.0 40.0 56-57 36.06386138330892 37.0 37.0 40.0 33.0 40.0 58-59 35.824235819092564 37.0 37.0 40.0 27.0 40.0 60-61 35.551456530810164 37.0 35.0 40.0 27.0 40.0 62-63 35.27887177689328 37.0 33.0 40.0 27.0 40.0 64-65 35.0109427009601 37.0 33.0 37.0 27.0 40.0 66-67 34.66793688779311 37.0 33.0 37.0 27.0 40.0 68-69 34.36099023898575 37.0 33.0 37.0 27.0 40.0 70-71 34.04311803720351 37.0 33.0 37.0 27.0 40.0 72-73 33.74965827876146 37.0 33.0 37.0 27.0 37.0 74-75 33.35087635731066 37.0 33.0 37.0 27.0 37.0 76-77 30.65675144757575 33.0 30.0 35.0 24.5 37.0 78-79 33.11330359745676 35.0 33.0 37.0 27.0 37.0 80-81 33.52621412498595 37.0 33.0 37.0 27.0 37.0 82-83 33.48141577576533 37.0 33.0 37.0 27.0 37.0 84-85 33.338663752779404 37.0 33.0 37.0 27.0 37.0 86-87 33.16794508071938 37.0 33.0 37.0 27.0 37.0 88-89 33.05420306644082 37.0 33.0 37.0 27.0 37.0 90-91 32.93192230819503 35.0 33.0 37.0 27.0 37.0 92-93 32.736413556053904 33.0 33.0 37.0 27.0 37.0 94-95 32.61374830187313 33.0 33.0 37.0 27.0 37.0 96-97 32.43640860219151 33.0 33.0 37.0 27.0 37.0 98-99 32.33916028221773 33.0 33.0 37.0 24.5 37.0 100-101 30.715563320840708 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1978183524856192 2306 2 0.17584469361027288 2306 3 0.14529375700622893 2306 4 0.17617944954961473 2306 5 0.15267743753677365 2306 6 0.1296789041906834 2306 7 0.23096089530493913 2306 8 0.3034112907012556 2306 9 0.15031692100133398 2306 10-11 0.19634215720796533 2306 12-13 0.25661161338728533 2306 14-15 0.5323765848878281 2306 16-17 0.47863646000472215 2306 18-19 0.5756702600642072 2306 20-21 0.5788444187300001 2306 22-23 0.6115807344194835 2306 24-25 0.5348071137391841 2306 26-27 0.6240121871144666 2306 28-29 0.7404568283099309 2306 30-31 0.626553895916679 2306 32-33 0.7162897142270666 2306 34-35 0.6885612392758702 2306 36-37 0.6390203730333894 2306 38-39 0.6427723991421246 2306 40-41 0.5985658366828588 2306 42-43 0.7063225873509609 2306 44-45 0.6803685846230536 2306 46-47 0.6085164635616565 2306 48-49 0.653213103003722 2306 50-51 0.652241644164647 2306 52-53 0.6670956755286426 2306 54-55 0.664352818164474 2306 56-57 0.7541923538095929 2306 58-59 0.7019794631612157 2306 60-61 0.6423805066298414 2306 62-63 0.6095745674350965 2306 64-65 0.696581380116811 2306 66-67 0.5694085856741893 2306 68-69 0.6924583117780188 2306 70-71 0.6878513440939571 2306 72-73 0.6130000799931423 2306 74-75 0.60187836483734 2306 76-77 0.22173466299823374 2306 78-79 0.724748267923097 2306 80-81 0.7983541107173977 2306 82-83 0.726475312991063 2306 84-85 0.8383001144619797 2306 86-87 0.9331358788666577 2306 88-89 0.7930906544690401 2306 90-91 0.8402534790438523 2306 92-93 0.7925457553936326 2306 94-95 0.7460512810100184 2306 96-97 0.6351298363088489 2306 98-99 0.6643733272280343 2306 100-101 0.6810478704145844 2307 1 0.12241602708328969 2307 2 0.06906483683041387 2307 3 0.09102795274042563 2307 4 0.12324189661205764 2307 5 0.1198176546769929 2307 6 0.17006544457722583 2307 7 0.2402394045834484 2307 8 0.169912407202375 2307 9 0.1706834413678564 2307 10-11 0.19706403292983765 2307 12-13 0.2528097345854121 2307 14-15 0.49115266866390783 2307 16-17 0.45179230816057725 2307 18-19 0.4722495316434774 2307 20-21 0.4148920047775846 2307 22-23 0.512789635628387 2307 24-25 0.4702906742227384 2307 26-27 0.3940129571152369 2307 28-29 0.4470672849203865 2307 30-31 0.3703650147278026 2307 32-33 0.44928232221966624 2307 34-35 0.4569642576788908 2307 36-37 0.3964316304446456 2307 38-39 0.4814379878077162 2307 40-41 0.49709898521600593 2307 42-43 0.3906511466795237 2307 44-45 0.382955787210264 2307 46-47 0.6324538624990623 2307 48-49 0.4692887940794179 2307 50-51 0.4352169271399333 2307 52-53 0.37591025934322175 2307 54-55 0.4334969623086167 2307 56-57 0.4255752751925215 2307 58-59 0.6557890419707917 2307 60-61 0.6063540956034288 2307 62-63 0.4008369836975163 2307 64-65 0.43444424297968 2307 66-67 0.37448289074848873 2307 68-69 0.30867067799038495 2307 70-71 0.3277701090127181 2307 72-73 0.3370510540441103 2307 74-75 0.4847901227490965 2307 76-77 0.22213891340248537 2307 78-79 0.5877266309014573 2307 80-81 0.5505486129118964 2307 82-83 0.6970901586059028 2307 84-85 0.6565414327032997 2307 86-87 0.5772607729915507 2307 88-89 0.5310690174474004 2307 90-91 0.4274271912175607 2307 92-93 0.42367688652475977 2307 94-95 0.373486408445153 2307 96-97 0.6426277188067289 2307 98-99 0.38824142609612977 2307 100-101 0.19488863425534575 2201 1 0.10457990002969098 2201 2 0.15438371307583765 2201 3 0.10647066381443437 2201 4 0.0892057000698614 2201 5 0.10149111882765283 2201 6 0.14684539303264188 2201 7 0.09014668103896639 2201 8 0.21375894216748748 2201 9 0.12470467335356261 2201 10-11 0.12530912872174582 2201 12-13 0.16471720134165224 2201 14-15 0.33579423774138917 2201 16-17 0.30620841413693256 2201 18-19 0.39228802909435956 2201 20-21 0.34995981382523667 2201 22-23 0.3183338980857542 2201 24-25 0.28936322733273556 2201 26-27 0.3979082350143557 2201 28-29 0.4987960420020059 2201 30-31 0.28355460854628944 2201 32-33 0.39656217528445126 2201 34-35 0.40532115994842854 2201 36-37 0.3609256491873438 2201 38-39 0.40874119115508023 2201 40-41 0.4267814281005613 2201 42-43 0.23941782021815783 2201 44-45 0.29498891971866925 2201 46-47 0.48966813389959896 2201 48-49 0.3641650673762342 2201 50-51 0.44912473797525365 2201 52-53 0.3890347616659966 2201 54-55 0.3314017081490306 2201 56-57 0.5012390330454295 2201 58-59 0.6006062787947499 2201 60-61 0.5605602220371111 2201 62-63 0.48195735034356346 2201 64-65 0.46702843542944095 2201 66-67 0.6378981302799076 2201 68-69 0.4879104073981395 2201 70-71 0.517006753242164 2201 72-73 0.5834751065171275 2201 74-75 0.4169655771281029 2201 76-77 0.14076012703089802 2201 78-79 0.6786426789706539 2201 80-81 0.5942932697146404 2201 82-83 0.5736056563407956 2201 84-85 0.6978746526250958 2201 86-87 0.6780391798189243 2201 88-89 0.6591131681751818 2201 90-91 0.4813021175120724 2201 92-93 0.5858889065140289 2201 94-95 0.4942824325056918 2201 96-97 0.7231392192654269 2201 98-99 0.7386902907072681 2201 100-101 0.6257317433269698 1101 1 0.11654160915675504 1101 2 0.06752979783609447 1101 3 0.055483334133917594 1101 4 0.13479579578810785 1101 5 0.08776279851790036 1101 6 0.1403801054456295 1101 7 0.11059160881187324 1101 8 0.1687199630131886 1101 9 0.1211126979860353 1101 10-11 0.1428101513143929 1101 12-13 0.10828282051452476 1101 14-15 0.08682861450272839 1101 16-17 0.18870721282792147 1101 18-19 0.13789411992650003 1101 20-21 0.22102992492202134 1101 22-23 0.18104712066111261 1101 24-25 0.2744668839364053 1101 26-27 0.22761377524373927 1101 28-29 0.21400348589568097 1101 30-31 0.20524724456117127 1101 32-33 0.29461544035409304 1101 34-35 0.28132328399232165 1101 36-37 0.2327423478628603 1101 38-39 0.2587541592606897 1101 40-41 0.1639361341378489 1101 42-43 0.25266974974046974 1101 44-45 0.20924170551572274 1101 46-47 0.31961438272146836 1101 48-49 0.3380245610236514 1101 50-51 0.18399455539638154 1101 52-53 0.239946002857792 1101 54-55 0.3217468920894859 1101 56-57 0.270763180641012 1101 58-59 0.24680921523852106 1101 60-61 0.1413324196697019 1101 62-63 0.2447098234986953 1101 64-65 0.3002986967820078 1101 66-67 0.22142557563905285 1101 68-69 0.15601313445336018 1101 70-71 0.13397638508870813 1101 72-73 0.15332125630779814 1101 74-75 0.10797158713577204 1101 76-77 -0.0201834461837187 1101 78-79 0.3015119611362991 1101 80-81 0.20341390258500525 1101 82-83 0.42263445581144055 1101 84-85 0.3556581855659786 1101 86-87 0.3046559205821353 1101 88-89 0.3853219441758924 1101 90-91 0.20323778543206572 1101 92-93 0.35710438102717035 1101 94-95 0.20211168332449603 1101 96-97 0.2016703584682631 1101 98-99 0.21353763901449696 1101 100-101 0.24228072154971514 2304 1 0.1650944331012525 2304 2 0.15102272755696333 2304 3 0.11756661827162418 2304 4 0.09522927578227325 2304 5 0.08958593041541718 2304 6 0.10657424815692451 2304 7 0.14660519201266453 2304 8 0.17389353385141249 2304 9 0.04318140575014695 2304 10-11 0.08210322323954955 2304 12-13 0.16427250332430532 2304 14-15 0.22500475769324169 2304 16-17 0.19623544532758785 2304 18-19 0.3446445479284179 2304 20-21 0.4059173118010335 2304 22-23 0.3264694535059647 2304 24-25 0.34285820443952275 2304 26-27 0.24833486297632135 2304 28-29 0.27945120802259993 2304 30-31 0.25167753060187437 2304 32-33 0.33120645717551866 2304 34-35 0.2955803924703986 2304 36-37 0.3660568059933311 2304 38-39 0.30233677838933914 2304 40-41 0.3211368757184445 2304 42-43 0.3708323540062466 2304 44-45 0.4365784397135073 2304 46-47 0.4847765676669127 2304 48-49 0.45264855309216045 2304 50-51 0.5310966221334326 2304 52-53 0.48543559624729227 2304 54-55 0.46064794724878055 2304 56-57 0.6854151056946662 2304 58-59 0.6333374859371474 2304 60-61 0.4992357832612413 2304 62-63 0.5730887597272769 2304 64-65 0.575463994074056 2304 66-67 0.6175596895622277 2304 68-69 0.430024541181929 2304 70-71 0.3666324321567629 2304 72-73 0.29039548989787534 2304 74-75 0.4133175307541137 2304 76-77 0.1355840749819741 2304 78-79 0.43977177305853843 2304 80-81 0.5548563254752068 2304 82-83 0.5965367133696233 2304 84-85 0.6920916751098858 2304 86-87 0.5703606826026544 2304 88-89 0.7185883931664065 2304 90-91 0.5952269872822811 2304 92-93 0.7520493354662392 2304 94-95 0.5873266479383759 2304 96-97 0.48342280493763923 2304 98-99 0.4696467586675084 2304 100-101 0.5254398010938282 2202 1 0.03446022159095463 2202 2 0.012734393652372944 2202 3 0.07007923784598802 2202 4 0.08380057637742055 2202 5 0.05381177405732984 2202 6 0.11942148119839402 2202 7 0.07631786300010646 2202 8 0.07137838257231977 2202 9 0.1188441281506698 2202 10-11 0.006299991226335067 2202 12-13 0.059614556108392946 2202 14-15 0.015186514618427793 2202 16-17 0.10423368481907147 2202 18-19 0.13905172005318178 2202 20-21 0.14215350427291185 2202 22-23 0.07927438760373917 2202 24-25 0.11110790063495557 2202 26-27 0.08687899643221897 2202 28-29 0.12705572847877278 2202 30-31 0.20721407673343606 2202 32-33 0.029022082857132148 2202 34-35 -0.09329393370664718 2202 36-37 0.06562378711070949 2202 38-39 -0.032813215378773464 2202 40-41 -0.009503859800197745 2202 42-43 0.0972799048740356 2202 44-45 0.1543899562435911 2202 46-47 0.17301728604786604 2202 48-49 0.08830060338350165 2202 50-51 0.08024002957430554 2202 52-53 0.07442589397577137 2202 54-55 -0.02691700071495262 2202 56-57 0.06175466961826004 2202 58-59 0.013298848227989879 2202 60-61 -0.03765076520727462 2202 62-63 -0.022633146140044857 2202 64-65 0.03676786096090723 2202 66-67 0.03030750450630393 2202 68-69 0.07599708581783204 2202 70-71 -0.13499087744346383 2202 72-73 -0.12181365617759354 2202 74-75 -0.13835651031403273 2202 76-77 -0.027910300738586358 2202 78-79 0.14451462975626583 2202 80-81 -0.0047003730260257726 2202 82-83 0.07067418104170287 2202 84-85 0.2305561572399455 2202 86-87 0.3245249473956022 2202 88-89 0.24309032894679206 2202 90-91 0.08653525544045237 2202 92-93 0.13351793843261817 2202 94-95 0.22861544144475232 2202 96-97 0.16471818087631362 2202 98-99 0.0031329355596696473 2202 100-101 0.10821856125959428 1102 1 0.06163253442728944 1102 2 0.07133922277014193 1102 3 0.02500774927002425 1102 4 0.066258219708061 1102 5 0.037477944448831124 1102 6 -0.01705474333977719 1102 7 0.04366434346655268 1102 8 0.05387844495326988 1102 9 0.0448237171017567 1102 10-11 -0.02566068381877784 1102 12-13 0.03138093526816732 1102 14-15 0.021360565804080522 1102 16-17 0.08012302931997795 1102 18-19 0.1566173656129024 1102 20-21 0.008649108494850566 1102 22-23 0.007416932247714669 1102 24-25 0.06938419004932683 1102 26-27 0.1270727100953053 1102 28-29 0.012769241857405689 1102 30-31 -0.010832318063158652 1102 32-33 -0.05557273203777413 1102 34-35 -0.003240369258804776 1102 36-37 0.06770190470296455 1102 38-39 0.01714062271353356 1102 40-41 0.08693543521182079 1102 42-43 0.06123826766504692 1102 44-45 0.05553693409419225 1102 46-47 -0.04814566132755971 1102 48-49 0.0010017819677017314 1102 50-51 -0.02428799611001864 1102 52-53 0.09372947297917023 1102 54-55 0.009181611100835596 1102 56-57 0.07040815588197091 1102 58-59 0.01663415002705193 1102 60-61 0.021573298870443125 1102 62-63 -0.07513420661630477 1102 64-65 -0.1298238099337965 1102 66-67 -0.03371115987584261 1102 68-69 -0.035496397451595385 1102 70-71 -0.0010481514788480695 1102 72-73 0.06373849846063706 1102 74-75 0.0845951070162343 1102 76-77 0.17236084848497413 1102 78-79 0.27227831405871683 1102 80-81 0.17920575611087486 1102 82-83 0.0927886459378584 1102 84-85 0.17365515997639136 1102 86-87 0.17862129686203332 1102 88-89 0.12529013099122466 1102 90-91 -0.1309351551925957 1102 92-93 -0.005650648181259044 1102 94-95 -0.005555182947041715 1102 96-97 -0.0404719146913024 1102 98-99 -0.23912059728182555 1102 100-101 -0.11079473836029408 2305 1 0.011816993199758485 2305 2 0.01595428603289406 2305 3 0.017902784395516846 2305 4 0.04417323658734773 2305 5 0.02265765736279235 2305 6 -0.022804757930600772 2305 7 0.0531637398670739 2305 8 0.09424035890118176 2305 9 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7 1.0 8 4.0 9 14.0 10 12.0 11 19.0 12 39.0 13 108.0 14 275.0 15 643.0 16 1322.0 17 1999.0 18 2858.0 19 3943.0 20 5057.0 21 6374.0 22 8057.0 23 10337.0 24 12782.0 25 15990.0 26 20350.0 27 26151.0 28 34425.0 29 46141.0 30 52723.0 31 63364.0 32 84027.0 33 119349.0 34 179814.0 35 307423.0 36 585435.0 37 841555.0 38 193197.0 39 427.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.42699833646081 26.071502035822387 21.807857598908832 14.693642028807966 2 32.533500494433575 22.703970520708097 31.186888269444385 13.575640715413941 3 37.69721611986823 18.887743572839877 26.284584151832075 17.13045615545982 4 31.793282209185197 18.665649824358404 31.577146049883964 17.963921916572435 5 31.245813032016446 17.2195179204135 33.8873277865296 17.64734126104045 6 27.097894036883414 17.86545690806584 37.189369011304336 17.847280043746416 7 33.80626206313126 18.85188523044034 27.66743578555873 19.67441692086967 8 30.999022564843198 26.707186720600255 23.4310450934851 18.862745621071443 9 29.08267805800973 25.51932673199414 25.66588484556334 19.732110364432792 10-11 24.085621795470264 28.25265841404001 30.53734164311994 17.124378147369786 12-13 23.78707537301631 27.10067582115033 29.95836850258077 19.153880303252592 14-15 22.731331083771718 27.81043092886825 30.744508357737455 18.713729629622573 16-17 22.52435490232317 27.936621046674908 31.299912545275443 18.239111505726473 18-19 21.598535180996983 27.862903763601686 31.65958200833392 18.87897904706741 20-21 22.140563940073505 28.060753406256726 31.227300354582226 18.571382299087535 22-23 21.40212536425262 28.71373812070609 30.909561700306643 18.97457481473465 24-25 21.702166171597984 27.431308029258272 31.393388117970517 19.473137681173228 26-27 21.516815504827157 27.69123337836267 30.981703099784124 19.810248017026044 28-29 21.431000728790064 28.687310955191318 30.48847987729652 19.39320843872209 30-31 20.564346107128397 28.982361207040274 30.71895586729226 19.734336818539074 32-33 20.323086300327663 29.613113072393183 30.93237705113253 19.131423576146624 34-35 21.125508677090888 28.936186104079365 31.00282789975951 18.935477319070245 36-37 20.47508609365908 29.1421843877147 31.795797310928343 18.586932207697878 38-39 21.09413466624594 29.19650628728047 30.430944334435456 19.278414712038135 40-41 21.015623421907854 28.55193407162161 30.8803357856131 19.552106720857438 42-43 20.89128804803992 29.096679106006228 30.806886636871955 19.205146209081896 44-45 20.522379599297224 28.485980966513342 31.405119610451248 19.586519823738186 46-47 21.15860767551577 28.387263706693254 30.696347060239088 19.757781557551887 48-49 20.735608600189362 29.901639006904762 30.098623944550017 19.26412844835586 50-51 21.482084004741345 29.084261395930376 29.64215643963882 19.791498159689453 52-53 20.850997582265478 28.852292579414808 29.48841514910379 20.808294689215927 54-55 21.825886571587784 28.905100002058205 29.676926689042656 19.59208673731136 56-57 21.24783895281828 29.123788746394442 29.509651915137546 20.118720385649734 58-59 21.016712460233155 29.285509833134572 30.023657150828342 19.674120555803928 60-61 21.108929209182943 29.487635896132154 29.93109094630552 19.472343948379386 62-63 20.636683652061972 29.31345009315862 29.711389284909906 20.3384769698695 64-65 21.116462904904214 28.967390051253105 29.738196988991927 20.17795005485075 66-67 21.2094899852392 29.182552713405073 29.421450903896606 20.186506397459123 68-69 21.306423108123766 29.709524492073854 29.20689718269483 19.777155217107552 70-71 20.55742102525503 30.759995341673214 28.97351558242713 19.709068050644625 72-73 20.375919243419734 30.519605761640317 28.97195299846883 20.132521996471112 74-75 20.87970043472493 29.76087460373854 29.01076062377157 20.348664337764955 76-77 20.824013466350134 29.25201301503224 29.31304003769114 20.610933480926484 78-79 20.64066243542278 29.31401369758338 29.52675099046512 20.518572876528715 80-81 20.073274633341008 29.759497462617627 29.492211021187792 20.675016882853576 82-83 19.593970570049756 29.525888063144045 29.58411765120591 21.296023715600292 84-85 19.7611686615327 29.325359585598854 29.92337161100636 20.99010014186208 86-87 20.014723981565865 29.29839827626885 30.56754602721211 20.11933171495317 88-89 20.193233805739386 29.890713777427802 29.91794671643936 19.998105700393456 90-91 20.461078296185008 29.604742118891416 29.43051048743695 20.50366909748663 92-93 19.890905764231423 30.076007654130866 29.570808334159228 20.462278247478483 94-95 19.55399439086147 30.394345500443603 29.65968748540776 20.39197262328717 96-97 19.83967580655736 30.928896092509945 29.229785177245166 20.00164292368753 98-99 20.402057397729955 30.237377532559677 28.79422992519264 20.566335144517726 100-101 20.42168955971966 30.0528946252543 28.26901838590874 21.256397429117296 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 393.0 1 244.5 2 111.0 3 170.0 4 205.0 5 168.0 6 136.0 7 133.5 8 154.0 9 161.5 10 147.5 11 150.5 12 167.0 13 169.0 14 159.5 15 176.0 16 187.5 17 188.5 18 212.0 19 271.5 20 365.5 21 477.5 22 679.5 23 1039.5 24 1555.0 25 2429.5 26 4181.0 27 6846.0 28 9729.0 29 12643.5 30 16163.5 31 19446.0 32 24925.0 33 36286.5 34 53393.5 35 77878.5 36 113168.5 37 152544.5 38 185535.0 39 202620.0 40 202643.5 41 188635.5 42 169947.5 43 160836.0 44 148279.0 45 132753.5 46 124027.5 47 110579.0 48 92555.5 49 76846.0 50 63139.5 51 51961.0 52 42306.5 53 33866.0 54 27799.0 55 21835.0 56 15430.0 57 10104.5 58 6824.5 59 4931.0 60 3645.5 61 2725.0 62 1949.0 63 1364.5 64 995.5 65 730.0 66 524.0 67 399.5 68 316.5 69 255.0 70 184.5 71 122.5 72 81.0 73 55.5 74 35.5 75 26.0 76 24.0 77 19.5 78 14.5 79 7.0 80 5.5 81 4.5 82 3.0 83 3.5 84 2.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14853965852645457 2 0.0 3 0.0 4 0.015471293320097628 5 3.8106633793343915E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.286398027600635E-4 24-25 0.0 26-27 0.0 28-29 9.526658448335979E-5 30-31 0.0010479324293169575 32-33 9.33612527936926E-4 34-35 2.286398027600635E-4 36-37 0.0014480520841470689 38-39 0.007125940519355312 40-41 0.015166440249750876 42-43 0.005620728484518227 44-45 0.0019243850065638677 46-47 5.144395562101428E-4 48-49 0.0059636881886583224 50-51 0.0024959845134640266 52-53 0.0103268977579962 54-55 0.02168267462841269 56-57 0.018557930657358487 58-59 0.018443610755978456 60-61 0.023321259881526477 62-63 0.007468900223495408 64-65 0.010536484243859593 66-67 0.027150976577757537 68-69 0.03806852715955057 70-71 0.036410888589540114 72-73 0.02766541613396768 74-75 0.03608698220229669 76-77 0.02998992079536166 78-79 0.012251282764560067 80-81 0.009564765082129322 82-83 0.004553742738304598 84-85 0.007144993836251983 86-87 0.023016406811179724 88-89 0.02111107512151253 90-91 0.015585613221477662 92-93 0.03029477386570841 94-95 0.03115217312605865 96-97 0.03212389228778892 98-99 0.023225993297043116 100-101 0.022959246860489706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2624215.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.84848585422761 #Duplication Level Percentage of deduplicated Percentage of total 1 87.52667783416985 41.00492328381644 2 5.445615779615207 5.102377076377234 3 1.9691813811046277 2.7675949823126564 4 1.0797351347237556 2.023358247416736 5 0.6979501922985285 1.634895485542653 6 0.4349700269269292 1.2226612292099548 7 0.29123733483488845 0.9550819712864654 8 0.2552682223213945 0.9567143761966144 9 0.2540330007641745 1.071095529852668 >10 1.697698796404166 15.843380998755002 >50 0.18847266050748543 6.139408022679558 >100 0.1432350971903284 14.464354820763598 >500 0.01354009590562326 4.093577742462243 >1k 0.002140547671989023 1.4775267172888376 >5k 1.6259704210831133E-4 0.3908881754967408 >10k+ 8.129851918247374E-5 0.8521613405425779 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 22374 0.8525978244922768 TruSeq Adapter, Index 2 (97% over 37bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5208 0.1984593487957351 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5055 0.19262903382535349 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.953862393134708E-4 0.0 0.0 2 5.715995069001587E-4 0.0 0.0 3 5.715995069001587E-4 0.0 0.0 4 5.715995069001587E-4 0.0 0.0 5 6.859194082801905E-4 0.0 0.0 6 7.621326758668783E-4 0.0 0.0 7 8.383459434535661E-4 0.0 0.0 8 0.0010669857462136296 0.0 0.0 9 0.0012194122813870053 0.0 0.0 10-11 0.0020387049079438994 0.0 0.0 12-13 0.003162850604847545 0.0 0.0 14-15 0.004534689421407926 0.0 0.0 16-17 0.0062494879421084015 0.0 0.0 18-19 0.00817387294867227 0.0 0.0 20-21 0.010326897757996202 0.0 0.0 22-23 0.012822882271460227 0.0 0.0 24-25 0.015909519608721084 0.0 0.0 26-27 0.019377223283915383 0.0 0.0 28-29 0.02250196725496958 0.0 0.0 30-31 0.03296223823124249 0.0 0.0 32-33 0.03799231389196388 0.0 0.0 34-35 0.0427365897992352 0.0 0.0 36-37 0.04679494629822632 0.0 0.0 38-39 0.051424902304117615 0.0 0.0 40-41 0.05704563078863584 0.0 0.0 42-43 0.06295215902660414 0.0 0.0 44-45 0.0686300474618124 0.0 0.0 46-47 0.075622614762891 0.0 0.0 48-49 0.08185304938810273 0.0 0.0 50-51 0.088864670006078 0.0 0.0 52-53 0.09650505008164346 0.0 0.0 54-55 0.10420259010789892 0.0 0.0 56-57 0.11382451514071827 0.0 0.0 58-59 0.12382750651147106 0.0 0.0 60-61 0.13423061753705395 0.0 0.0 62-63 0.1455292344567804 0.0 0.0 64-65 0.15854264989720734 0.0 0.0 66-67 0.17321370390764476 0.0 0.0 68-69 0.18885647707981243 0.0 0.0 70-71 0.20749062100475762 0.0 0.0 72-73 0.2291732956331703 0.0 0.0 74-75 0.2512751432333098 0.0 0.0 76-77 0.27768304045209713 0.0 0.0 78-79 0.30700609515607524 0.0 0.0 80-81 0.3394920004649009 0.0 3.8106633793343915E-5 82-83 0.37832266030031836 0.0 3.8106633793343915E-5 84-85 0.42168800955714375 0.0 3.8106633793343915E-5 86-87 0.46951183496779036 0.0 3.8106633793343915E-5 88-89 0.5242139077781356 0.0 3.8106633793343915E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 4440 0.0 53.594894 3 CGGAAGA 4485 0.0 52.845352 4 CGGCGAG 45 1.5050835E-5 52.845245 1 GAAGAGC 4725 0.0 50.051083 6 GATCGGA 4880 0.0 49.12009 1 GCGTACC 40 5.3909764E-4 47.497276 2 ATCGGAA 5040 0.0 47.403034 2 TATGCCG 2795 0.0 42.743122 48-49 CGGCGCG 45 9.566997E-4 42.276196 1 CGTATGC 2825 0.0 42.198704 46-47 TGCCGTC 2815 0.0 42.18954 50-51 GCCGTCT 2815 0.0 41.936405 50-51 ATGCCGT 2850 0.0 41.668247 48-49 TCGTATG 2880 0.0 41.14389 44-45 CTCGTAT 2960 0.0 40.031895 44-45 CTTGACG 60 8.2314706E-5 39.633934 1 CCGTCTT 2995 0.0 39.416393 52-53 TCTCGTA 3005 0.0 39.353394 42-43 CGTCTTC 2990 0.0 39.323425 52-53 GTATGCC 3065 0.0 38.739445 46-47 >>END_MODULE